Restriction map for
pFN21AB6924
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvrII BamHI BbrPI BbsI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BseAI BsgI BsiHKAI BsiWI BsmAI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstF5I BstPI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FokI FseI HincII HindII HindIII
HpaI KpnI Ksp632I MamI MboII
MfeI MflI MluI MroI MseI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp509I Tth111I Van91I XbaI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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ApaI 298 GGGCCC GGGCC^C
AvaI 219 CCCGGG C^[CT]CG[GA]G
AviII 629 TGCGCA TGC^GCA
BalI 356 TGGCCA TGG^CCA
BanII 298 GGGCCC G[GA]GC[CT]^C
BbeI 422 GGCGCC GGCGC^C
BbvCI 55 CCTCAGC CCTCAGC(-5/-2)
BcgI 501 GCAGATGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 237 GGATAC GTATCC(6/5)
BfaI 148 CTAG C^TAG
BsmI 76 GCATTC GAATGC(1/-1)
BsoBI 219 CCCGGG C^[CT]CG[GA]G
BspHI 539 TCATGA T^CATGA
BstAPI 270 GCAGGACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DdeI 56 CTCAG C^T[ACGT]AG
DraII 493 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 356 TGGCCA [CT]^GGCC[GA]
EcoNI 519 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 493 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 10 CCAAGG C^C[AT][AT]GG
FspI 629 TGCGCA TGC^GCA
HaeII 422 GGCGCC [GA]GCGC^[CT]
HphI 264 GGTGA GGTGA(8/7)
KasI 422 GGCGCC G^GCGCC
KspI 157 CCGCGG CCGC^GG
MaeI 148 CTAG C^TAG
MaeII 548 ACGT A^CGT
MluNI 356 TGGCCA TGG^CCA
MscI 356 TGGCCA TGG^CCA
NarI 422 GGCGCC GG^CGCC
PpuMI 493 AGGACCT [GA]G^G[AT]CC[CT]
PstI 61 CTGCAG CTGCA^G
PvuII 58 CAGCTG CAG^CTG
RcaI 539 TCATGA T^CATGA
SacII 157 CCGCGG CCGC^GG
SfoI 422 GGCGCC GGC^GCC
SmaI 219 CCCGGG CCC^GGG
SmlI 168 CTCAAG C^T[CT][GA]AG
SphI 350 GCATGC GCATG^C
StyI 10 CCAAGG C^C[AT][AT]GG
XcmI 182 CCACACTCATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 219 CCCGGG C^CCGGG
XmaI 219 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 255 GGCGTC G[GA]^CG[CT]C
422 GGCGCC
AfaI 621 GTAC GT^AC
675 GTAC
BanI 422 GGCGCC G^G[CT][GA]CC
597 GGTGCC
BpmI 390 CTGGAG CTGGAG(16/14)
520 CTGGAG
BsaHI 255 GGCGTC G[GA]^CG[CT]C
422 GGCGCC
BsaWI 225 ACCGGA [AT]^CCGG[AT]
488 ACCGGA
BsiEI 115 CGGTCG CG[GA][CT]^CG
486 CGACCG
BsmFI 140 GGGAC GGGAC(10/14)
222 GGGAC
BsrI 389 ACTGG ACTGG(1/-1)
557 ACTGG
BstNI 86 CCAGG CC^[AT]GG
519 CCTGG
DpnII 378 GATC ^GATC
609 GATC
DsaI 157 CCGCGG C^C[GA][CT]GG
660 CCACGG
EcoRII 86 CCAGG ^CC[AT]GG
519 CCTGG
FauI 109 CCCGC CCCGC(4/6)
590 CCCGC
HgaI 256 GCGTC GACGC(5/10)
327 GCGTC
Hin1I 255 GGCGTC G[GA]^CG[CT]C
422 GGCGCC
HinfI 17 GAGTC G^A[ACGT]TC
571 GAGTC
MboI 378 GATC ^GATC
609 GATC
MslI 192 CATGGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
534 CATCATCATG
MvaI 86 CCAGG CC^[AT]GG
519 CCTGG
NdeII 378 GATC ^GATC
609 GATC
NspI 275 ACATGC [GA]CATG^[CT]
350 GCATGC
PleI 17 GAGTC GAGTC(4/5)
571 GAGTC
RsaI 621 GTAC GT^AC
675 GTAC
Sau3AI 378 GATC ^GATC
609 GATC
SfaNI 504 GATGC GCATC(5/9)
452 GCATC
SfcI 61 CTGCAG C^T[GA][CT]AG
345 CTACAG
Tsp45I 19 GTCAC ^GT[GC]AC
209 GTCAC
TspRI 388 CACTG CA[GC]TG[ACGT][ACGT]^
467 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AccII AciI AluI AvaII BbvI
BcnI BmyI BsaJI BseRI BsiYI
BslI Bsp1286I BssKI BstUI Cac8I
CfoI Cfr13I Fnu4HI HaeIII HapII
HhaI HinP1I HpaII ItaI MaeIII
MnlI MspA1I MspI MvnI MwoI
NciI NlaIII NlaIV Sau96I ScrFI
TaqI TseI TthHB8I