Restriction map for
pFN21AB6911
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# NO CUT ENZYMES ( 150 enzymes) #
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Acc65I AccI AccII AciI AclI
AcsI AflII AflIII AgeI AhdI
AluI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BlnI BlpI BpmI Bpu1102I
BsaAI BsaI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsoBI
BspDI BspLU11I BsrBI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraII DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HgaI
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MfeI MluI
MspA1I MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TfiI Tsp509I Tth111I Van91I
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 175 GACGTC GACGT^C
AccIII 477 TCCGGA T^CCGGA
AcyI 175 GACGTC G[GA]^CG[CT]C
BanI 418 GGCACC G^G[CT][GA]CC
BbsI 342 GAAGAC GAAGAC(2/6)
BfaI 370 CTAG C^TAG
BglI 28 GCCGTCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 122 AGATCT A^GATCT
BpuAI 342 GAAGAC GAAGAC(2/6)
BsaBI 475 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 175 GACGTC G[GA]^CG[CT]C
BsaWI 477 TCCGGA [AT]^CCGG[AT]
BseAI 477 TCCGGA T^CCGGA
BsiYI 38 CCATGAAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 38 CCATGAAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 453 GTCCC GGGAC(10/14)
BsmI 516 GAATGC GAATGC(1/-1)
Bsp1407I 146 TGTACA T^GTACA
BspEI 477 TCCGGA T^CCGGA
BspHI 260 TCATGA T^CATGA
BspMI 421 ACCTGC ACCTGC(4/8)
BsrDI 324 CATTGC GCAATG(2/0)
BsrFI 140 ACCGGC [GA]^CCGG[CT]
BsrGI 146 TGTACA T^GTACA
BsrI 319 CCAGT ACTGG(1/-1)
BstF5I 531 GGATG GGATG(2/0)
Cac8I 165 GCTCGC GC[ACGT]^[ACGT]GC
Cfr10I 140 ACCGGC [GA]^CCGG[CT]
DdeI 314 CTGAG C^T[ACGT]AG
DrdI 403 GACTTTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FokI 531 GGATG GGATG(9/13)
Hin1I 175 GACGTC G[GA]^CG[CT]C
MaeI 370 CTAG C^TAG
MaeII 176 ACGT A^CGT
MamI 475 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 477 TCCGGA T^CCGGA
MseI 350 TTAA T^TAA
PstI 68 CTGCAG CTGCA^G
RcaI 260 TCATGA T^CATGA
SfaNI 532 GATGC GCATC(5/9)
SfcI 68 CTGCAG C^T[GA][CT]AG
SfiI 27 GGCCGTCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 199 CTCAAG C^T[CT][GA]AG
SspBI 146 TGTACA T^GTACA
TaqI 569 TCGA T^CGA
Tru9I 350 TTAA T^TAA
TthHB8I 569 TCGA T^CGA
XbaI 369 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 24 enzymes) #
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AfaI 147 GTAC GT^AC
438 GTAC
AvaII 336 GGACC G^G[AT]CC
525 GGACC
BamHI 86 GGATCC G^GATCC
474 GGATCC
BanII 163 GGGCTC G[GA]GC[CT]^C
463 GAGCCC
BbvI 67 GCTGC GCAGC(8/12)
383 GCTGC
BmyI 163 GGGCTC G[AGT]GC[ACT]^C
463 GAGCCC
Bsp1286I 163 GGGCTC G[AGT]GC[ACT]^C
463 GAGCCC
BstEII 102 GGTGACC G^GT[ACGT]ACC
136 GGTGACC
BstNI 90 CCAGG CC^[AT]GG
590 CCAGG
BstPI 102 GGTGACC G^GT[ACGT]ACC
136 GGTGACC
EcoO65I 102 GGTGACC G^GT[ACGT]ACC
136 GGTGACC
EcoRII 90 CCAGG ^CC[AT]GG
590 CCAGG
EcoT14I 131 CCATGG C^C[AT][AT]GG
389 CCATGG
Fnu4HI 67 GCTGC GC^[ACGT]GC
383 GCTGC
HaeII 77 GGCGCT [GA]GCGC^[CT]
194 AGCGCC
HinfI 451 GAGTC G^A[ACGT]TC
551 GAGTC
ItaI 67 GCTGC GC^[ACGT]GC
383 GCTGC
MslI 33 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
261 CATGAGCATG
MvaI 90 CCAGG CC^[AT]GG
590 CCAGG
NcoI 131 CCATGG C^CATGG
389 CCATGG
PleI 451 GAGTC GAGTC(4/5)
551 GAGTC
RsaI 147 GTAC GT^AC
438 GTAC
StyI 131 CCATGG C^C[AT][AT]GG
389 CCATGG
TseI 67 GCTGC G^C[AT]GC
383 GCTGC
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AlwI BalI BcnI BsaJI BssKI
BstYI CfoI Cfr13I DpnII DsaI
EaeI HaeIII HapII HhaI HinP1I
HpaII HphI MaeIII MboI MboII
MflI MluNI MnlI MscI MspI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI Tsp45I TspRI
XhoII