(Circular) (Six-base) MAPSORT of: pFN21AB6911.seq Check: 3293 from: 1 to: 5320 pFN21AB6911 5320 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2435 2435 Size: 5320 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2184 3130 278 Size: 53 83 186 936 648 946 2468 Fragments arranged by size: 2468 946 936 648 186 83 53 AccI GT'mk_AC Cuts at: 1937 2663 1937 Size: 726 4594 Acc65I G'GTAC_C Cuts at: 2641 2641 Size: 5320 AclI AA'CG_TT Cuts at: 1563 3447 3820 1563 Size: 1884 373 3063 Fragments arranged by size: 3063 1884 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2624 3383 4767 5035 1632 Size: 992 759 1384 268 1917 Fragments arranged by size: 1917 1384 992 759 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5123 AflIII A'CryG_T Cuts at: 1203 4219 5196 1203 Size: 3016 977 1327 Fragments arranged by size: 3016 1327 977 AgeI A'CCGG_T Cuts at: 4151 4151 Size: 5320 AhdI GACnn_n'nnGTC Cuts at: 4049 4049 Size: 5320 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5320 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5320 AlwNI CAG_nnn'CTG Cuts at: 3063 4635 3063 Size: 1572 3748 ApaLI G'TGCA_C Cuts at: 3377 4533 3377 Size: 1156 4164 ApoI r'AATT_y Cuts at: 1493 2629 2855 3007 5116 1493 Size: 1136 226 152 2109 1697 Fragments arranged by size: 2109 1697 1136 226 152 AseI AT'TA_AT Cuts at: 160 1032 3874 160 Size: 872 2842 1606 Fragments arranged by size: 2842 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5320 AvaI C'yCGr_G Cuts at: 1870 1943 2645 1870 Size: 73 702 4545 Fragments arranged by size: 4545 702 73 BamHI G'GATC_C Cuts at: 2091 2479 2650 3017 2091 Size: 388 171 367 4394 Fragments arranged by size: 4394 388 367 171 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2423 2641 4096 618 Size: 325 217 627 636 218 1455 1842 Fragments arranged by size: 1842 1455 636 627 325 218 217 BanII G_rGCy'C Cuts at: 1391 2172 2472 2639 1391 Size: 781 300 167 4072 Fragments arranged by size: 4072 781 300 167 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2354 928 Size: 971 455 3894 Fragments arranged by size: 3894 971 455 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3511 3545 1903 Size: 34 1574 34 3678 Fragments arranged by size: 3678 1574 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3212 4422 3212 Size: 1210 4110 BclI T'GATC_A Cuts at: 1548 1548 Size: 5320 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2039 3931 136 Size: 107 122 71 631 605 367 1892 1525 Fragments arranged by size: 1892 1525 631 605 367 122 107 71 BglII A'GATC_T Cuts at: 2127 5315 2127 Size: 3188 2132 BlpI GC'TnA_GC Cuts at: 2737 2737 Size: 5320 Bme1580I G_kGCm'C Cuts at: 1790 3381 4537 1790 Size: 1591 1156 2573 Fragments arranged by size: 2573 1591 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5320 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3051 4009 442 Size: 416 53 83 399 24 1634 958 1753 Fragments arranged by size: 1753 1634 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5320 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3980 1239 Size: 2741 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2189 3445 4310 4608 2189 Size: 1256 865 298 2901 Fragments arranged by size: 2901 1256 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3983 882 Size: 617 2484 2219 Fragments arranged by size: 2484 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3111 493 Size: 711 1907 2702 Fragments arranged by size: 2702 1907 711 BsaBI GATnn'nnATC Cuts at: 2484 5314 2484 Size: 2830 2490 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2181 3127 3509 4144 Size: 53 83 186 936 648 946 382 635 Cuts at: 4144 275 Size: 1451 Fragments arranged by size: 1451 946 936 648 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2482 3753 4151 4425 4572 4983 2482 Size: 1271 398 274 147 411 2819 Fragments arranged by size: 2819 1271 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2019 2049 2547 2577 1178 Size: 30 165 30 616 30 498 30 3921 Fragments arranged by size: 3921 616 498 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5320 BseYI C'CCAG_C Cuts at: 2196 4523 5019 2196 Size: 2327 496 2497 Fragments arranged by size: 2497 2327 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4747 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2770 3531 4559 1540 Size: 275 186 769 761 1028 2301 Fragments arranged by size: 2301 1028 769 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3381 3466 4537 3381 Size: 85 1071 4164 Fragments arranged by size: 4164 1071 85 BsmI GAATG_Cn' Cuts at: 2527 2831 2924 2527 Size: 304 93 4923 Fragments arranged by size: 4923 304 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2172 2472 2639 3381 3466 4537 1391 Size: 399 382 300 167 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 382 300 167 85 BspEI T'CCGG_A Cuts at: 2482 4983 2482 Size: 2501 2819 BspHI T'CATG_A Cuts at: 2265 3209 4939 2265 Size: 944 1730 2646 Fragments arranged by size: 2646 1730 944 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2435 2675 844 Size: 1591 240 3489 Fragments arranged by size: 3489 1591 240 BsrBI CCG'CTC Cuts at: 1394 1823 3207 1394 Size: 429 1384 3507 Fragments arranged by size: 3507 1384 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2328 3815 3989 65 Size: 1183 1080 1487 174 1396 Fragments arranged by size: 1487 1396 1183 1080 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2145 3964 4151 1700 Size: 110 335 1819 187 2869 Fragments arranged by size: 2869 1819 335 187 110 BsrGI T'GTAC_A Cuts at: 96 2151 96 Size: 2055 3265 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5320 BssSI C'ACGA_G Cuts at: 3380 4392 3380 Size: 1012 4308 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5320 BstBI TT'CG_AA Cuts at: 4124 4124 Size: 5320 BstEII G'GTnAC_C Cuts at: 1184 2107 2141 1184 Size: 923 34 4363 Fragments arranged by size: 4363 923 34 BstYI r'GATC_y Cuts at: 1972 2091 2127 2479 2650 3017 3402 3419 4871 Size: 119 36 352 171 367 385 17 1452 Cuts at: 4871 4957 4969 5315 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 352 346 171 119 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2112 2136 2394 513 Size: 552 1047 24 258 3439 Fragments arranged by size: 3439 1047 552 258 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2053 487 Size: 683 56 827 3754 Fragments arranged by size: 3754 827 683 56 BtsI GCAGTG_nn' Cuts at: 2547 2836 3630 3650 2547 Size: 289 794 20 4217 Fragments arranged by size: 4217 794 289 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5320 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5285 DraI TTT'AAA Cuts at: 2624 2624 Size: 5320 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2414 4327 809 Size: 507 1098 1913 1802 Fragments arranged by size: 1913 1802 1098 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2064 2163 Size: 54 1425 50 275 18 210 24 99 Cuts at: 2163 2277 2767 3656 5285 8 Size: 114 490 889 1629 43 Fragments arranged by size: 1629 1425 889 490 275 210 114 99 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 2767 1537 Size: 275 955 4090 Fragments arranged by size: 4090 955 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3250 1353 Size: 1897 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3907 4281 4427 387 Size: 1064 138 2318 374 146 1280 Fragments arranged by size: 2318 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2624 3383 3980 4767 5035 1239 Size: 393 992 759 597 787 268 1524 Fragments arranged by size: 1524 992 787 759 597 393 268 EcoRI G'AATT_C Cuts at: 2629 3007 2629 Size: 378 4942 FspI TGC'GCA Cuts at: 3826 3826 Size: 5320 HaeII r_GCGC'y Cuts at: 1678 2086 2203 4467 1678 Size: 408 117 2264 2531 Fragments arranged by size: 2531 2264 408 117 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2019 2051 2528 2547 2560 Size: 32 135 32 447 32 477 19 13 Cuts at: 2560 2579 3961 3993 4035 4067 1373 Size: 19 1382 32 42 32 2626 Fragments arranged by size: 2626 1382 477 447 135 42 32 32 32 32 32 19 19 13 HincII GTy'rAC Cuts at: 669 1657 1938 2664 2910 669 Size: 988 281 726 246 3079 Fragments arranged by size: 3079 988 726 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5320 HpaI GTT'AAC Cuts at: 2910 2910 Size: 5320 KpnI G_GTAC'C Cuts at: 2645 2645 Size: 5320 MfeI C'AATT_G Cuts at: 2919 2919 Size: 5320 MluI A'CGCG_T Cuts at: 5196 5196 Size: 5320 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2319 4434 4618 1305 Size: 1014 2115 184 2007 Fragments arranged by size: 2115 2007 1014 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2042 2066 2279 10 Size: 54 1425 343 210 24 213 3051 Fragments arranged by size: 3051 1425 343 213 210 54 24 MslI CAynn'nnrTG Cuts at: 518 1166 2042 2270 3278 3637 3796 518 Size: 648 876 228 1008 359 159 2042 Fragments arranged by size: 2042 1008 876 648 359 228 159 MspA1I CmG'CkG Cuts at: 1606 2736 3413 4561 4806 1606 Size: 1130 677 1148 245 2120 Fragments arranged by size: 2120 1148 1130 677 245 NcoI C'CATG_G Cuts at: 513 1065 2136 2394 513 Size: 552 1071 258 3439 Fragments arranged by size: 3439 1071 552 258 NdeI CA'TA_TG Cuts at: 387 387 Size: 5320 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5320 NotI GC'GGCC_GC Cuts at: 2767 2767 Size: 5320 NspI r_CATG'y Cuts at: 1132 1229 2678 4223 1132 Size: 97 1449 1545 2229 Fragments arranged by size: 2229 1545 1449 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5320 PciI A'CATG_T Cuts at: 4219 4219 Size: 5320 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5320 PmeI GTTT'AAAC Cuts at: 2624 2624 Size: 5320 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3389 3421 4902 4934 3389 Size: 32 1481 32 3775 Fragments arranged by size: 3775 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5320 PsiI TTA'TAA Cuts at: 2890 2890 Size: 5320 PstI C_TGCA'G Cuts at: 830 2077 2672 830 Size: 1247 595 3478 Fragments arranged by size: 3478 1247 595 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5320 SalI G'TCGA_C Cuts at: 1936 2662 1936 Size: 726 4594 SbfI CC_TGCA'GG Cuts at: 2672 2672 Size: 5320 ScaI AGT'ACT Cuts at: 3568 5280 3568 Size: 1712 3608 SfcI C'TryA_G Cuts at: 826 1045 2073 2668 3803 4484 4675 826 Size: 219 1028 595 1135 681 191 1471 Fragments arranged by size: 1471 1135 1028 681 595 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2039 2039 Size: 5320 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5320 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5320 SmaI CCC'GGG Cuts at: 1872 2647 1872 Size: 775 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2204 3424 4325 4587 820 Size: 197 926 261 1220 901 262 1553 Fragments arranged by size: 1553 1220 926 901 262 261 197 SnaBI TAC'GTA Cuts at: 493 3111 493 Size: 2618 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5320 SphI G_CATG'C Cuts at: 1132 2678 1132 Size: 1546 3774 SspI AAT'ATT Cuts at: 5 52 3244 5 Size: 47 3192 2081 Fragments arranged by size: 3192 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5320 StyI C'CwwG_G Cuts at: 513 1065 2136 2394 2759 513 Size: 552 1071 258 365 3074 Fragments arranged by size: 3074 1071 552 365 258 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2129 2147 3347 3364 3517 3702 4147 Size: 1255 549 18 1200 17 153 185 445 Cuts at: 4147 325 Size: 1498 Fragments arranged by size: 1498 1255 1200 549 445 185 153 18 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2151 3566 5278 Size: 274 80 33 51 1443 174 1415 1712 Cuts at: 5278 96 Size: 138 Fragments arranged by size: 1712 1443 1415 274 174 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2044 2076 5280 5312 2044 Size: 32 3204 32 2052 Fragments arranged by size: 3204 2052 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5320 XbaI T'CTAG_A Cuts at: 2374 2656 2374 Size: 282 5038 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5320 XmaI C'CCGG_G Cuts at: 1870 2645 1870 Size: 775 4545 XmnI GAAnn'nnTTC Cuts at: 3449 3449 Size: 5320 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2182 3128 276 Size: 53 83 186 936 648 946 2468 Fragments arranged by size: 2468 946 936 648 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI XcmI