Restriction map for pFN21AB6896
 (Circular) (Six-base) MAPSORT of: pFN21AB6896.seq  Check: 6223  from: 1  to: 5311



pFN21AB6896  5311 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:41  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2045    2045 
   Size:       5311
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3121     278 
   Size:         53      83     186     936    1585    2468

  Fragments arranged by size: 

                2468    1585     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2654    1937 
   Size:        717    4594
 
Acc65I G'GTAC_C

Cuts at:   2632    2632 
   Size:       5311
 
AclI AA'CG_TT

Cuts at:   1563    3438    3811    1563 
   Size:       1875     373    3063

  Fragments arranged by size: 

                3063    1875     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2273    2540    3374    4758    5026    1632 
   Size:        641     267     834    1384     268    1917

  Fragments arranged by size: 

                1917    1384     834     641     268     267
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5114
 
AflIII A'CryG_T

Cuts at:   1203    2066    2480    4210    5187    1203 
   Size:        863     414    1730     977    1327

  Fragments arranged by size: 

                1730    1327     977     863     414
 
AgeI A'CCGG_T

Cuts at:   4142    4142 
   Size:       5311
 
AhdI GACnn_n'nnGTC

Cuts at:   4040    4040 
   Size:       5311
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5311
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5311
 
AlwNI CAG_nnn'CTG

Cuts at:   3054    4626    3054 
   Size:       1572    3739
 
ApaLI G'TGCA_C

Cuts at:   3368    4524    3368 
   Size:       1156    4155
 
ApoI r'AATT_y

Cuts at:   1493    2620    2846    2998    5107    1493 
   Size:       1127     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1127     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3865     160 
   Size:        872    2833    1606

  Fragments arranged by size: 

                2833    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5311
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2636    1870 
   Size:         73     693    4545

  Fragments arranged by size: 

                4545     693      73
 
BamHI G'GATC_C

Cuts at:   2641    3008    2641 
   Size:        367    4944
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2632    4087     618 
   Size:        325     217     627     845    1455    1842

  Fragments arranged by size: 

                1842    1455     845     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2601    2630    1391 
   Size:       1210      29    4072

  Fragments arranged by size: 

                4072    1210      29
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2261    2483     928 
   Size:        971     362     222    3756

  Fragments arranged by size: 

                3756     971     362     222
 
BbvCI CC'TCA_GC

Cuts at:   2557    2557 
   Size:       5311
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3502    3536    1903 
   Size:         34    1565      34    3678

  Fragments arranged by size: 

                3678    1565      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3203    4413    3203 
   Size:       1210    4101
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5311
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2342    3922     136 
   Size:        107     122      71     631     605     670    1580    1525

  Fragments arranged by size: 

                1580    1525     670     631     605     122     107      71
            
 
BglII A'GATC_T

Cuts at:   2352    5306    2352 
   Size:       2954    2357
 
BlpI GC'TnA_GC

Cuts at:   2213    2728    2213 
   Size:        515    4796
 
Bme1580I G_kGCm'C

Cuts at:   1790    2347    3372    4528    1790 
   Size:        557    1025    1156    2573

  Fragments arranged by size: 

                2573    1156    1025     557
 
BmgBI CAC'GTC

Cuts at:   1318    2069    1318 
   Size:        751    4560
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2121    3042    4000 
   Size:        416      53      83     399      24     704     921     958

Cuts at:   4000     442 
   Size:       1753

  Fragments arranged by size: 

                1753     958     921     704     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5311
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2518    2518 
   Size:       5311
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2063    2300    2323    3971    1239 
   Size:        824     237      23    1648    2579

  Fragments arranged by size: 

                2579    1648     824     237      23
 
Bpu10I CC'TnA_GC

Cuts at:   2469    2557    2469 
   Size:         88    5223
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3436    4301    4599    3436 
   Size:        865     298    4148

  Fragments arranged by size: 

                4148     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2402    3974     882 
   Size:        617     903    1572    2219

  Fragments arranged by size: 

                2219    1572     903     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2483    3102     493 
   Size:        711    1279     619    2702

  Fragments arranged by size: 

                2702    1279     711     619
 
BsaBI GATnn'nnATC

Cuts at:   5305    5305 
   Size:       5311
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3118    3500    4135     275 
   Size:         53      83     186     936    1585     382     635    1451

  Fragments arranged by size: 

                1585    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3744    4142    4416    4563    4974    3744 
   Size:        398     274     147     411    4081

  Fragments arranged by size: 

                4081     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5086

  Fragments arranged by size: 

                5086     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2285    2315    2375    1186 
   Size:       1099      30      60    4122

  Fragments arranged by size: 

                4122    1099      60      30
 
BseYI C'CCAG_C

Cuts at:   2098    2182    4514    5010    2098 
   Size:         84    2332     496    2399

  Fragments arranged by size: 

                2399    2332     496      84
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4738
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2761    3522    4550    1540 
   Size:        275     186     760     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     760     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3372    3457    4528    3372 
   Size:         85    1071    4155

  Fragments arranged by size: 

                4155    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2822    2915    2822 
   Size:         93    5218
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2347    2601    2630    3372    3457    4528    1391 
   Size:        399     557     254      29     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     557     399     254      85      29
            
 
BspEI T'CCGG_A

Cuts at:   4974    4974 
   Size:       5311
 
BspHI T'CATG_A

Cuts at:   3200    4930    3200 
   Size:       1730    3581
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2045    2214    2442    2666     844 
   Size:       1201     169     228     224    3489

  Fragments arranged by size: 

                3489    1201     228     224     169
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3198    1394 
   Size:        429    1375    3507

  Fragments arranged by size: 

                3507    1375     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3806    3980      65 
   Size:       1183    2558     174    1396

  Fragments arranged by size: 

                2558    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3955    4142    1700 
   Size:        110    2145     187    2869

  Fragments arranged by size: 

                2869    2145     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5311
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5311
 
BssSI C'ACGA_G

Cuts at:   3371    4383    3371 
   Size:       1012    4299
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5311
 
BstBI TT'CG_AA

Cuts at:   4115    4115 
   Size:       5311
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5311
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2041    2041 
   Size:       5311
 
BstYI r'GATC_y

Cuts at:   1972    2076    2313    2352    2641    3008    3393    3410    4862 
   Size:        104     237      39     289     367     385      17    1452

Cuts at:   4862    4948    4960    5306    1972 
   Size:         86      12     346    1977

  Fragments arranged by size: 

                1977    1452     385     367     346     289     237     104
                  86      39      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    4759
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4572

  Fragments arranged by size: 

                4572     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2380    2827    3621    3641    2380 
   Size:        447     794      20    4050

  Fragments arranged by size: 

                4050     794     447      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5311
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2440    2475     529 
   Size:         35    1876      35    3365

  Fragments arranged by size: 

                3365    1876      35      35
 
DraI TTT'AAA

Cuts at:   2615    2615 
   Size:       5311
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4318     809 
   Size:        507    3002    1802

  Fragments arranged by size: 

                3002    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2019    2758    3647 
   Size:         54    1425      50     275      18     189     739     889

Cuts at:   3647    5276       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     739     275     189      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2758    1537 
   Size:        275     946    4090

  Fragments arranged by size: 

                4090     946     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3241    1353 
   Size:       1888    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3898    4272    4418     387 
   Size:       1064     138    2309     374     146    1280

  Fragments arranged by size: 

                2309    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2063    2273    2300    2323    2540    3374    3971 
   Size:        393     431     210      27      23     217     834     597

Cuts at:   3971    4758    5026    1239 
   Size:        787     268    1524

  Fragments arranged by size: 

                1524     834     787     597     431     393     268     217
                 210      27      23
 
EcoO109I rG'GnC_Cy

Cuts at:   2380    2539    2380 
   Size:        159    5152
 
EcoRI G'AATT_C

Cuts at:   2620    2998    2620 
   Size:        378    4933
 
FspI TGC'GCA

Cuts at:   3817    3817 
   Size:       5311
 
HaeII r_GCGC'y

Cuts at:   1678    4458    1678 
   Size:       2780    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2585    2617    3952    3984    4026 
   Size:         32     135      32    1013      32    1335      32      42

Cuts at:   4026    4058    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1335    1013     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2395    2655    2901     669 
   Size:        988     281     457     260     246    3079

  Fragments arranged by size: 

                3079     988     457     281     260     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5311
 
HpaI GTT'AAC

Cuts at:   2901    2901 
   Size:       5311
 
KpnI G_GTAC'C

Cuts at:   2636    2636 
   Size:       5311
 
MfeI C'AATT_G

Cuts at:   2910    2910 
   Size:       5311
 
MluI A'CGCG_T

Cuts at:   5187    5187 
   Size:       5311
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4425    4609    1305 
   Size:       3120     184    2007

  Fragments arranged by size: 

                3120    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3489

  Fragments arranged by size: 

                3489    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3269    3628    3787     518 
   Size:        648    2103     359     159    2042

  Fragments arranged by size: 

                2103    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2583    2727    3404    4552    4797    1606 
   Size:        977     144     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     977     677     245     144
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4759
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5311
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5311
 
NotI GC'GGCC_GC

Cuts at:   2758    2758 
   Size:       5311
 
NspI r_CATG'y

Cuts at:   1132    1229    2457    2669    4214    1132 
   Size:         97    1228     212    1545    2229

  Fragments arranged by size: 

                2229    1545    1228     212      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5311
 
PciI A'CATG_T

Cuts at:   4210    4210 
   Size:       5311
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5311
 
PmeI GTTT'AAAC

Cuts at:   2615    2615 
   Size:       5311
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3380    3412    4893    4925    3380 
   Size:         32    1481      32    3766

  Fragments arranged by size: 

                3766    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2539    2539 
   Size:       5311
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5311
 
PsiI TTA'TAA

Cuts at:   2881    2881 
   Size:       5311
 
PstI C_TGCA'G

Cuts at:    830    2439    2588    2663     830 
   Size:       1609     149      75    3478

  Fragments arranged by size: 

                3478    1609     149      75
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5311
 
PvuII CAG'CTG

Cuts at:   2583    2583 
   Size:       5311
 
SalI G'TCGA_C

Cuts at:   1936    2653    1936 
   Size:        717    4594
 
SbfI CC_TGCA'GG

Cuts at:   2663    2663 
   Size:       5311
 
ScaI AGT'ACT

Cuts at:   2119    3559    5271    2119 
   Size:       1440    1712    2159

  Fragments arranged by size: 

                2159    1712    1440
 
SfcI C'TryA_G

Cuts at:    826    1045    2435    2584    2659    3794    4475    4666     826 
   Size:        219    1390     149      75    1135     681     191    1471

  Fragments arranged by size: 

                1471    1390    1135     681     219     191     149      75
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5311
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5311
 
SmaI CCC'GGG

Cuts at:   1872    2638    1872 
   Size:        766    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3415    4316    4578     820 
   Size:        197     926    1472     901     262    1553

  Fragments arranged by size: 

                1553    1472     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3102     493 
   Size:       2609    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5311
 
SphI G_CATG'C

Cuts at:   1132    2669    1132 
   Size:       1537    3774
 
SspI AAT'ATT

Cuts at:      5      52    3235       5 
   Size:         47    3183    2081

  Fragments arranged by size: 

                3183    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5311
 
StyI C'CwwG_G

Cuts at:    513    1065    2750     513 
   Size:        552    1685    3074

  Fragments arranged by size: 

                3074    1685     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3338    3355    3508    3693    4138     325 
   Size:       1255    1758      17     153     185     445    1498

  Fragments arranged by size: 

                1758    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2117    3557    5269 
   Size:        274      80      33      51    1443     140    1440    1712

Cuts at:   5269      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1440     274     140     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5271    5303    5271 
   Size:         32    5279
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5311
 
XbaI T'CTAG_A

Cuts at:   2647    2647 
   Size:       5311
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2038    2587    2038 
   Size:        549    4762
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5311
 
XmaI C'CCGG_G

Cuts at:   1870    2636    1870 
   Size:        766    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3440    3440 
   Size:       5311
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3119     276 
   Size:         53      83     186     936    1585    2468

  Fragments arranged by size: 

                2468    1585     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI  Eco57MI EcoO109I    EcoRI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI
     PasI     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI
     PstI     PvuI    PvuII     SalI     SbfI     ScaI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BfrBI
    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII  EcoICRI    EcoNI    EcoRV
     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI
     NsiI     PacI     PfoI     PmlI   PspOMI    PspXI     PsrI    RsrII
     SacI    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI
     SwaI
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