Restriction map for
pFN21AB6881
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaII
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HaeII HgaI Hin1I HindIII HpaI
KasI KpnI KspI MaeI MaeII
MfeI MluI MroI MseI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NlaIII NlaIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Tsp509I
Tth111I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 274 GTCGAC GT^[AC][GT]AC
AlwI 384 GGATC GGATC(4/5)
AlwNI 382 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 343 GAGCTC G[AT]GC[AT]^C
AvaI 308 CCCGGG C^[CT]CG[GA]G
AviII 53 TGCGCA TGC^GCA
BanII 343 GAGCTC G[GA]GC[CT]^C
BbvI 406 GCTGC GCAGC(8/12)
BcgI 435 GCATTCCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 343 GAGCTC G[AGT]GC[ACT]^C
BpmI 231 CTGGAG CTGGAG(16/14)
BsaBI 51 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 343 GAGCTC G[AT]GC[AT]^C
BsmFI 488 GGGAC GGGAC(10/14)
BsoBI 308 CCCGGG C^[CT]CG[GA]G
Bsp1286I 343 GAGCTC G[AGT]GC[ACT]^C
BsrDI 281 GCAATG GCAATG(2/0)
BstYI 384 GGATCT [GA]^GATC[CT]
CfoI 54 GCGC GCG^C
DdeI 410 CTGAG C^T[ACGT]AG
EarI 313 GAAGAG CTCTTC(1/4)
EcoT14I 255 CCAAGG C^C[AT][AT]GG
FauI 485 GCGGG CCCGC(4/6)
FspI 53 TGCGCA TGC^GCA
HhaI 54 GCGC GCG^C
HinP1I 54 GCGC G^CGC
HincII 274 GTCGAC GT[CT]^[GA]AC
HindII 274 GTCGAC GT[CT]^[GA]AC
HinfI 219 GATTC G^A[ACGT]TC
Ksp632I 313 GAAGAG CTCTTC(1/4)
MamI 51 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 384 GGATCT [GA]^GATC[CT]
MslI 57 CATCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 483 CAGCGG C[AC]G^C[GT]G
SacI 343 GAGCTC GAGCT^C
SalI 274 GTCGAC G^TCGAC
SapI 313 GAAGAGC GCTCTTC(1/4)
SmaI 308 CCCGGG CCC^GGG
SmlI 25 CTCAAG C^T[CT][GA]AG
StuI 504 AGGCCT AGG^CCT
StyI 255 CCAAGG C^C[AT][AT]GG
TfiI 219 GATTC G^A[AT]TC
TseI 406 GCTGC G^C[AT]GC
XhoII 384 GGATCT [GA]^GATC[CT]
XmaCI 308 CCCGGG C^CCGGG
XmaI 308 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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BalI 207 TGGCCA TGG^CCA
518 TGGCCA
BsaJI 255 CCAAGG C^C[ACGT][ACGT]GG
308 CCCGGG
BsmAI 160 GAGAC GTCTC(1/5)
234 GAGAC
BsmI 326 GAATGC GAATGC(1/-1)
435 GCATTC
EaeI 207 TGGCCA [CT]^GGCC[GA]
518 TGGCCA
Fnu4HI 79 GCCGC GC^[ACGT]GC
406 GCTGC
HapII 309 CCGG C^CGG
348 CCGG
HpaII 309 CCGG C^CGG
348 CCGG
HphI 128 TCACC GGTGA(8/7)
447 TCACC
ItaI 79 GCCGC GC^[ACGT]GC
406 GCTGC
MboII 42 GAAGA GAAGA(8/7)
313 GAAGA
MluNI 207 TGGCCA TGG^CCA
518 TGGCCA
MscI 207 TGGCCA TGG^CCA
518 TGGCCA
MspI 309 CCGG C^CGG
348 CCGG
TaqI 222 TCGA T^CGA
275 TCGA
Tsp45I 127 GTCAC ^GT[GC]AC
446 GTCAC
TspRI 164 CAGTG CA[GC]TG[ACGT][ACGT]^
191 CAGTG
TthHB8I 222 TCGA T^CGA
275 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AfaI AluI BcnI BsiYI
BslI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DpnII EcoRII FokI
HaeIII MaeIII MboI MnlI MvaI
MwoI NciI NdeII RsaI Sau3AI
Sau96I ScrFI SfaNI