(Circular) (Six-base) MAPSORT of: pFN21AB6701.seq Check: 9134 from: 1 to: 4891 pFN21AB6701 4891 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2024 2057 2024 Size: 33 4858 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2701 278 Size: 53 83 186 936 1165 2468 Fragments arranged by size: 2468 1165 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2234 1937 Size: 297 4594 Acc65I G'GTAC_C Cuts at: 2212 2212 Size: 4891 AclI AA'CG_TT Cuts at: 1563 3018 3391 1563 Size: 1455 373 3063 Fragments arranged by size: 3063 1455 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2954 4338 4606 1632 Size: 1322 1384 268 1917 Fragments arranged by size: 1917 1384 1322 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4694 AflIII A'CryG_T Cuts at: 1203 3790 4767 1203 Size: 2587 977 1327 Fragments arranged by size: 2587 1327 977 AgeI A'CCGG_T Cuts at: 3722 3722 Size: 4891 AhdI GACnn_n'nnGTC Cuts at: 3620 3620 Size: 4891 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4891 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4891 AlwNI CAG_nnn'CTG Cuts at: 2061 2184 2634 4206 2061 Size: 123 450 1572 2746 Fragments arranged by size: 2746 1572 450 123 ApaLI G'TGCA_C Cuts at: 2948 4104 2948 Size: 1156 3735 ApoI r'AATT_y Cuts at: 1493 2200 2426 2578 4687 1493 Size: 707 226 152 2109 1697 Fragments arranged by size: 2109 1697 707 226 152 AseI AT'TA_AT Cuts at: 160 1032 3445 160 Size: 872 2413 1606 Fragments arranged by size: 2413 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4891 AvaI C'yCGr_G Cuts at: 1870 1943 2216 1870 Size: 73 273 4545 Fragments arranged by size: 4545 273 73 BamHI G'GATC_C Cuts at: 2221 2588 2221 Size: 367 4524 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2212 3667 618 Size: 325 217 627 425 1455 1842 Fragments arranged by size: 1842 1455 627 425 325 217 BanII G_rGCy'C Cuts at: 1391 2210 1391 Size: 819 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 3920 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3082 3116 1903 Size: 34 1145 34 3678 Fragments arranged by size: 3678 1145 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2783 3993 2783 Size: 1210 3681 BclI T'GATC_A Cuts at: 1548 1548 Size: 4891 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2124 3502 136 Size: 107 122 71 631 605 452 1378 1525 Fragments arranged by size: 1525 1378 631 605 452 122 107 71 BglII A'GATC_T Cuts at: 4886 4886 Size: 4891 BlpI GC'TnA_GC Cuts at: 2308 2308 Size: 4891 Bme1580I G_kGCm'C Cuts at: 1790 2143 2952 4108 1790 Size: 353 809 1156 2573 Fragments arranged by size: 2573 1156 809 353 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4891 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2622 3580 442 Size: 416 53 83 399 24 1205 958 1753 Fragments arranged by size: 1753 1205 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4891 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3551 1239 Size: 2312 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3016 3881 4179 3016 Size: 865 298 3728 Fragments arranged by size: 3728 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3554 882 Size: 617 2055 2219 Fragments arranged by size: 2219 2055 617 BsaAI yAC'GTr Cuts at: 493 1204 2682 493 Size: 711 1478 2702 Fragments arranged by size: 2702 1478 711 BsaBI GATnn'nnATC Cuts at: 4885 4885 Size: 4891 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2698 3080 3715 275 Size: 53 83 186 936 1165 382 635 1451 Fragments arranged by size: 1451 1165 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3324 3722 3996 4143 4554 3324 Size: 398 274 147 411 3661 Fragments arranged by size: 3661 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4666 Fragments arranged by size: 4666 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4891 BseYI C'CCAG_C Cuts at: 4094 4590 4094 Size: 496 4395 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2029 2197 1163 Size: 573 293 168 3857 Fragments arranged by size: 3857 573 293 168 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2341 3102 4130 1540 Size: 275 186 340 761 1028 2301 Fragments arranged by size: 2301 1028 761 340 275 186 BsiHKAI G_wGCw'C Cuts at: 2952 3037 4108 2952 Size: 85 1071 3735 Fragments arranged by size: 3735 1071 85 BsmI GAATG_Cn' Cuts at: 2402 2495 2402 Size: 93 4798 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2143 2210 2952 3037 4108 1391 Size: 399 353 67 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 353 85 67 BspEI T'CCGG_A Cuts at: 4554 4554 Size: 4891 BspHI T'CATG_A Cuts at: 2780 4510 2780 Size: 1730 3161 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2024 2057 2246 844 Size: 1180 33 189 3489 Fragments arranged by size: 3489 1180 189 33 BsrBI CCG'CTC Cuts at: 1394 1823 2778 1394 Size: 429 955 3507 Fragments arranged by size: 3507 955 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3386 3560 65 Size: 1183 2138 174 1396 Fragments arranged by size: 2138 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3535 3722 1700 Size: 110 1725 187 2869 Fragments arranged by size: 2869 1725 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4891 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4891 BssSI C'ACGA_G Cuts at: 2951 3963 2951 Size: 1012 3879 BstAPI GCAn_nnn'nTGC Cuts at: 794 2076 2091 2184 794 Size: 1282 15 93 3501 Fragments arranged by size: 3501 1282 93 15 BstBI TT'CG_AA Cuts at: 3695 3695 Size: 4891 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4891 BstYI r'GATC_y Cuts at: 1972 2221 2588 2973 2990 4442 4528 4540 4886 Size: 249 367 385 17 1452 86 12 346 Cuts at: 4886 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 346 249 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3400 Fragments arranged by size: 3400 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4152 Fragments arranged by size: 4152 683 56 BtsI GCAGTG_nn' Cuts at: 2407 3201 3221 2407 Size: 794 20 4077 Fragments arranged by size: 4077 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4891 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4856 DraI TTT'AAA Cuts at: 2195 2195 Size: 4891 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3898 809 Size: 507 2582 1802 Fragments arranged by size: 2582 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2338 3227 4856 Size: 54 1425 50 275 18 508 889 1629 Cuts at: 4856 8 Size: 43 Fragments arranged by size: 1629 1425 889 508 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2338 1537 Size: 275 526 4090 Fragments arranged by size: 4090 526 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2093 2821 1353 Size: 740 728 3423 Fragments arranged by size: 3423 740 728 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3478 3852 3998 387 Size: 1064 138 1889 374 146 1280 Fragments arranged by size: 1889 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2954 3551 4338 4606 1239 Size: 393 1322 597 787 268 1524 Fragments arranged by size: 1524 1322 787 597 393 268 EcoRI G'AATT_C Cuts at: 2200 2578 2200 Size: 378 4513 FspI TGC'GCA Cuts at: 3397 3397 Size: 4891 HaeII r_GCGC'y Cuts at: 1678 4038 1678 Size: 2360 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3532 3564 3606 3638 1373 Size: 32 135 32 1960 32 42 32 2626 Fragments arranged by size: 2626 1960 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2235 2481 669 Size: 988 281 297 246 3079 Fragments arranged by size: 3079 988 297 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4891 HpaI GTT'AAC Cuts at: 2481 2481 Size: 4891 KpnI G_GTAC'C Cuts at: 2216 2216 Size: 4891 MfeI C'AATT_G Cuts at: 2490 2490 Size: 4891 MluI A'CGCG_T Cuts at: 4767 4767 Size: 4891 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4005 4189 1305 Size: 2700 184 2007 Fragments arranged by size: 2700 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3069 Fragments arranged by size: 3069 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2849 3208 3367 518 Size: 648 1683 359 159 2042 Fragments arranged by size: 2042 1683 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2030 2181 2307 2984 4132 4377 1606 Size: 424 151 126 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 424 245 151 126 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3400 Fragments arranged by size: 3400 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 4891 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4891 NotI GC'GGCC_GC Cuts at: 2338 2338 Size: 4891 NspI r_CATG'y Cuts at: 1132 1229 2249 3794 1132 Size: 97 1020 1545 2229 Fragments arranged by size: 2229 1545 1020 97 PasI CC'CwG_GG Cuts at: 1348 2143 1348 Size: 795 4096 PciI A'CATG_T Cuts at: 3790 3790 Size: 4891 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4891 PmeI GTTT'AAAC Cuts at: 2195 2195 Size: 4891 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2960 2992 4473 4505 2960 Size: 32 1481 32 3346 Fragments arranged by size: 3346 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4891 PsiI TTA'TAA Cuts at: 2461 2461 Size: 4891 PstI C_TGCA'G Cuts at: 830 2035 2243 830 Size: 1205 208 3478 Fragments arranged by size: 3478 1205 208 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4891 PvuII CAG'CTG Cuts at: 2181 2181 Size: 4891 SalI G'TCGA_C Cuts at: 1936 2233 1936 Size: 297 4594 SapI GCTCTTCn'nnn_ Cuts at: 2093 2093 Size: 4891 SbfI CC_TGCA'GG Cuts at: 2243 2243 Size: 4891 ScaI AGT'ACT Cuts at: 3139 4851 3139 Size: 1712 3179 SfcI C'TryA_G Cuts at: 826 1045 2031 2239 3374 4055 4246 826 Size: 219 986 208 1135 681 191 1471 Fragments arranged by size: 1471 1135 986 681 219 208 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4891 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4891 SmaI CCC'GGG Cuts at: 1872 2218 1872 Size: 346 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2995 3896 4158 820 Size: 197 926 1052 901 262 1553 Fragments arranged by size: 1553 1052 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2682 493 Size: 2189 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4891 SphI G_CATG'C Cuts at: 1132 2249 1132 Size: 1117 3774 SspI AAT'ATT Cuts at: 5 52 2815 5 Size: 47 2763 2081 Fragments arranged by size: 2763 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4891 StyI C'CwwG_G Cuts at: 513 1065 2004 2330 513 Size: 552 939 326 3074 Fragments arranged by size: 3074 939 552 326 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2918 2935 3088 3273 3718 325 Size: 1255 1338 17 153 185 445 1498 Fragments arranged by size: 1498 1338 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3137 4849 96 Size: 274 80 33 51 1443 1160 1712 138 Fragments arranged by size: 1712 1443 1160 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4851 4883 4851 Size: 32 4859 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4891 XbaI T'CTAG_A Cuts at: 2227 2227 Size: 4891 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4891 XmaI C'CCGG_G Cuts at: 1870 2216 1870 Size: 346 4545 XmnI GAAnn'nnTTC Cuts at: 3020 3020 Size: 4891 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2699 276 Size: 53 83 186 936 1165 2468 Fragments arranged by size: 2468 1165 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI