Restriction map for
pFN21AB6569
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AlwI Aor51HI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsiYI BslI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MaeII MaeIII MamI MfeI MluI
MroI MseI MslI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfoI SfuI SgrAI SmlI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TaqI Tru9I Tsp45I Tsp509I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 148 CGCG CG^CG
AfaI 712 GTAC GT^AC
Alw44I 840 GTGCAC G^TGCAC
ApaLI 840 GTGCAC G^TGCAC
Asp700I 786 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 768 TGGCCA TGG^CCA
BbsI 69 GAAGAC GAAGAC(2/6)
BglII 1058 AGATCT A^GATCT
BpuAI 69 GAAGAC GAAGAC(2/6)
BseRI 13 CTCCTC GAGGAG(10/8)
BsgI 20 CTGCAC GTGCAG(16/14)
BspMI 687 GCAGGT ACCTGC(4/8)
BstAPI 547 GCAGGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 148 CGCG CG^CG
BstYI 1058 AGATCT [GA]^GATC[CT]
DpnII 1059 GATC ^GATC
DsaI 563 CCATGG C^C[GA][CT]GG
EaeI 768 TGGCCA [CT]^GGCC[GA]
EcoT14I 563 CCATGG C^C[AT][AT]GG
FauI 1037 GCGGG CCCGC(4/6)
HgaI 1104 GCGTC GACGC(5/10)
MboI 1059 GATC ^GATC
MflI 1058 AGATCT [GA]^GATC[CT]
MluNI 768 TGGCCA TGG^CCA
MscI 768 TGGCCA TGG^CCA
MvnI 148 CGCG CG^CG
NcoI 563 CCATGG C^CATGG
NdeII 1059 GATC ^GATC
NruI 147 TCGCGA TCG^CGA
NspI 452 GCATGC [GA]CATG^[CT]
PstI 212 CTGCAG CTGCA^G
RsaI 712 GTAC GT^AC
SacI 754 GAGCTC GAGCT^C
Sau3AI 1059 GATC ^GATC
SfaNI 228 GATGC GCATC(5/9)
SfcI 212 CTGCAG C^T[GA][CT]AG
SfiI 478 GGCCTCGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 600 CCCGGG CCC^GGG
SphI 452 GCATGC GCATG^C
StyI 563 CCATGG C^C[AT][AT]GG
TfiI 949 GATTC G^A[AT]TC
XhoII 1058 AGATCT [GA]^GATC[CT]
XmaCI 600 CCCGGG C^CCGGG
XmaI 600 CCCGGG C^CCGGG
XmnI 786 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AocI 391 CCTCAGG CC^T[ACGT]AGG
490 CCTAAGG
AspHI 754 GAGCTC G[AT]GC[AT]^C
840 GTGCAC
AvaI 176 CTCGGG C^[CT]CG[GA]G
600 CCCGGG
BanI 442 GGTGCC G^G[CT][GA]CC
586 GGTGCC
BglI 173 GCCCTCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
479 GCCTCGCAGGC
BsiHKAI 754 GAGCTC G[AT]GC[AT]^C
840 GTGCAC
BsmAI 299 GAGAC GTCTC(1/5)
632 GAGAC
BsmI 888 GAATGC GAATGC(1/-1)
623 GCATTC
BsoBI 176 CTCGGG C^[CT]CG[GA]G
600 CCCGGG
BstF5I 159 GGATG GGATG(2/0)
227 GGATG
Bsu36I 391 CCTCAGG CC^T[ACGT]AGG
490 CCTAAGG
Eco81I 391 CCTCAGG CC^T[ACGT]AGG
490 CCTAAGG
FokI 159 GGATG GGATG(9/13)
227 GGATG
HapII 332 CCGG C^CGG
601 CCGG
HpaII 332 CCGG C^CGG
601 CCGG
MspI 332 CCGG C^CGG
601 CCGG
PleI 524 GAGTC GAGTC(4/5)
988 GAGTC
SapI 6 GCTCTTC GCTCTTC(1/4)
756 GCTCTTC
StuI 477 AGGCCT AGG^CCT
695 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI AlwNI ApaI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BsmFI Bsp1286I BsrI BssKI
BstNI Cac8I Cfr13I DdeI DraII
EarI EcoO109I EcoRII Fnu4HI HaeIII
HinfI HphI ItaI Ksp632I MboII
MnlI MspA1I MvaI MwoI NciI
NlaIII NlaIV PpuMI PvuII Sau96I
ScrFI TseI TspRI