(Circular) (Six-base) MAPSORT of: pFN21AB6539.seq Check: 3626 from: 1 to: 5809 pFN21AB6539 5809 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3619 278 Size: 53 83 186 936 2083 2468 Fragments arranged by size: 2468 2083 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3152 1937 Size: 1215 4594 Acc65I G'GTAC_C Cuts at: 3130 3130 Size: 5809 AclI AA'CG_TT Cuts at: 1563 3936 4309 1563 Size: 2373 373 3063 Fragments arranged by size: 3063 2373 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3872 5256 5524 1632 Size: 2240 1384 268 1917 Fragments arranged by size: 2240 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5612 AflIII A'CryG_T Cuts at: 1203 4708 5685 1203 Size: 3505 977 1327 Fragments arranged by size: 3505 1327 977 AgeI A'CCGG_T Cuts at: 4640 4640 Size: 5809 AhdI GACnn_n'nnGTC Cuts at: 4538 4538 Size: 5809 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5809 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5809 AlwNI CAG_nnn'CTG Cuts at: 2781 3552 5124 2781 Size: 771 1572 3466 Fragments arranged by size: 3466 1572 771 ApaI G_GGCC'C Cuts at: 2479 2620 2806 2479 Size: 141 186 5482 Fragments arranged by size: 5482 186 141 ApaLI G'TGCA_C Cuts at: 3866 5022 3866 Size: 1156 4653 ApoI r'AATT_y Cuts at: 1493 3118 3344 3496 5605 1493 Size: 1625 226 152 2109 1697 Fragments arranged by size: 2109 1697 1625 226 152 AseI AT'TA_AT Cuts at: 160 1032 4363 160 Size: 872 3331 1606 Fragments arranged by size: 3331 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5809 AvaI C'yCGr_G Cuts at: 1870 1943 3134 1870 Size: 73 1191 4545 Fragments arranged by size: 4545 1191 73 BamHI G'GATC_C Cuts at: 3139 3506 3139 Size: 367 5442 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2216 2414 3130 4585 618 Size: 325 217 627 429 198 716 1455 1842 Fragments arranged by size: 1842 1455 716 627 429 325 217 198 BanII G_rGCy'C Cuts at: 1391 2341 2479 2516 2620 2806 3068 3128 1391 Size: 950 138 37 104 186 262 60 4072 Fragments arranged by size: 4072 950 262 186 138 104 60 37 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4838 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4000 4034 1903 Size: 34 2063 34 3678 Fragments arranged by size: 3678 2063 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2161 3701 4911 2161 Size: 1540 1210 3059 Fragments arranged by size: 3059 1540 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5809 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4420 136 Size: 107 122 71 631 605 2748 1525 Fragments arranged by size: 2748 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5804 5804 Size: 5809 BlpI GC'TnA_GC Cuts at: 3226 3226 Size: 5809 Bme1580I G_kGCm'C Cuts at: 1790 2479 2620 2806 3870 5026 1790 Size: 689 141 186 1064 1156 2573 Fragments arranged by size: 2573 1156 1064 689 186 141 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5809 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2791 3540 4498 Size: 416 53 83 399 24 1374 749 958 Cuts at: 4498 442 Size: 1753 Fragments arranged by size: 1753 1374 958 749 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5809 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2419 4469 1239 Size: 1180 2050 2579 Fragments arranged by size: 2579 2050 1180 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2872 3934 4799 5097 2872 Size: 1062 865 298 3584 Fragments arranged by size: 3584 1062 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4472 882 Size: 617 2973 2219 Fragments arranged by size: 2973 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2898 3600 493 Size: 711 1694 702 2702 Fragments arranged by size: 2702 1694 711 702 BsaBI GATnn'nnATC Cuts at: 2288 5803 2288 Size: 3515 2294 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2196 3616 3998 4633 Size: 53 83 186 936 663 1420 382 635 Cuts at: 4633 275 Size: 1451 Fragments arranged by size: 1451 1420 936 663 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2382 4242 4640 4914 5061 5472 2382 Size: 1860 398 274 147 411 2719 Fragments arranged by size: 2719 1860 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5584 Fragments arranged by size: 5584 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2443 2744 2747 2768 1186 Size: 1257 301 3 21 4227 Fragments arranged by size: 4227 1257 301 21 3 BseYI C'CCAG_C Cuts at: 2472 2613 5012 5508 2472 Size: 141 2399 496 2773 Fragments arranged by size: 2773 2399 496 141 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5236 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3259 4020 5048 1540 Size: 275 186 1258 761 1028 2301 Fragments arranged by size: 2301 1258 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2375 2516 3870 3955 5026 2375 Size: 141 1354 85 1071 3158 Fragments arranged by size: 3158 1354 1071 141 85 BsmI GAATG_Cn' Cuts at: 3320 3413 3320 Size: 93 5716 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2341 2375 2479 2516 2620 2806 3068 Size: 399 551 34 104 37 104 186 262 Cuts at: 3068 3128 3870 3955 5026 1391 Size: 60 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 551 399 262 186 104 104 85 60 37 34 BspEI T'CCGG_A Cuts at: 5472 5472 Size: 5809 BspHI T'CATG_A Cuts at: 2308 3698 5428 2308 Size: 1390 1730 2689 Fragments arranged by size: 2689 1730 1390 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2240 3097 3164 844 Size: 1396 857 67 3489 Fragments arranged by size: 3489 1396 857 67 BsrBI CCG'CTC Cuts at: 1394 1823 3696 1394 Size: 429 1873 3507 Fragments arranged by size: 3507 1873 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2278 2608 4304 4478 65 Size: 1183 1030 330 1696 174 1396 Fragments arranged by size: 1696 1396 1183 1030 330 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4453 4640 1700 Size: 110 2643 187 2869 Fragments arranged by size: 2869 2643 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5809 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5809 BssSI C'ACGA_G Cuts at: 2432 3869 4881 2432 Size: 1437 1012 3360 Fragments arranged by size: 3360 1437 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5809 BstBI TT'CG_AA Cuts at: 4613 4613 Size: 5809 BstEII G'GTnAC_C Cuts at: 1184 2998 1184 Size: 1814 3995 BstXI CCAn_nnnn'nTGG Cuts at: 2971 2971 Size: 5809 BstYI r'GATC_y Cuts at: 1972 2283 3139 3506 3891 3908 5360 5446 5458 Size: 311 856 367 385 17 1452 86 12 Cuts at: 5458 5804 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 856 385 367 346 311 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2300 2300 Size: 5809 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5257 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2818 487 Size: 683 56 1592 3478 Fragments arranged by size: 3478 1592 683 56 BtsI GCAGTG_nn' Cuts at: 3035 3325 4119 4139 3035 Size: 290 794 20 4705 Fragments arranged by size: 4705 794 290 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5809 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5774 DraI TTT'AAA Cuts at: 3113 3113 Size: 5809 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2249 2387 4816 809 Size: 507 933 138 2429 1802 Fragments arranged by size: 2429 1802 933 507 138 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2740 3256 4145 Size: 54 1425 50 275 18 910 516 889 Cuts at: 4145 5774 8 Size: 1629 43 Fragments arranged by size: 1629 1425 910 889 516 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3256 1537 Size: 275 1444 4090 Fragments arranged by size: 4090 1444 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2067 2420 3739 1353 Size: 714 353 1319 3423 Fragments arranged by size: 3423 1319 714 353 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4396 4770 4916 387 Size: 1064 138 2807 374 146 1280 Fragments arranged by size: 2807 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2514 2514 Size: 5809 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2419 3872 4469 5256 5524 1239 Size: 393 787 1453 597 787 268 1524 Fragments arranged by size: 1524 1453 787 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3008 3008 Size: 5809 EcoO109I rG'GnC_Cy Cuts at: 2315 2476 2617 2803 2315 Size: 161 141 186 5321 Fragments arranged by size: 5321 186 161 141 EcoRI G'AATT_C Cuts at: 3118 3496 3118 Size: 378 5431 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2114 2114 Size: 5809 FspI TGC'GCA Cuts at: 2270 4315 2270 Size: 2045 3764 HaeII r_GCGC'y Cuts at: 1678 4956 1678 Size: 3278 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2268 2300 2639 2671 2870 Size: 32 135 32 696 32 339 32 199 Cuts at: 2870 2902 4450 4482 4524 4556 1373 Size: 32 1548 32 42 32 2626 Fragments arranged by size: 2626 1548 696 339 199 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3052 3153 3399 669 Size: 988 281 1114 101 246 3079 Fragments arranged by size: 3079 1114 988 281 246 101 HindIII A'AGCT_T Cuts at: 748 748 Size: 5809 HpaI GTT'AAC Cuts at: 3399 3399 Size: 5809 KpnI G_GTAC'C Cuts at: 3134 3134 Size: 5809 MfeI C'AATT_G Cuts at: 3408 3408 Size: 5809 MluI A'CGCG_T Cuts at: 5685 5685 Size: 5809 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4923 5107 1305 Size: 3618 184 2007 Fragments arranged by size: 3618 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3987 Fragments arranged by size: 3987 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2436 3767 4126 4285 518 Size: 648 1270 1331 359 159 2042 Fragments arranged by size: 2042 1331 1270 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2613 2841 3225 3902 5050 5295 1606 Size: 1007 228 384 677 1148 245 2120 Fragments arranged by size: 2120 1148 1007 677 384 245 228 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5257 NdeI CA'TA_TG Cuts at: 387 387 Size: 5809 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5809 NotI GC'GGCC_GC Cuts at: 3256 3256 Size: 5809 NspI r_CATG'y Cuts at: 1132 1229 2441 2945 3167 4712 1132 Size: 97 1212 504 222 1545 2229 Fragments arranged by size: 2229 1545 1212 504 222 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5809 PciI A'CATG_T Cuts at: 4708 4708 Size: 5809 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5809 PmeI GTTT'AAAC Cuts at: 3113 3113 Size: 5809 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3878 3910 5391 5423 3878 Size: 32 1481 32 4264 Fragments arranged by size: 4264 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2315 2315 Size: 5809 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5809 PsiI TTA'TAA Cuts at: 3379 3379 Size: 5809 PspOMI G'GGCC_C Cuts at: 2475 2616 2802 2475 Size: 141 186 5482 Fragments arranged by size: 5482 186 141 PstI C_TGCA'G Cuts at: 830 3161 830 Size: 2331 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5809 PvuII CAG'CTG Cuts at: 2613 2841 2613 Size: 228 5581 SacI G_AGCT'C Cuts at: 2516 2516 Size: 5809 SalI G'TCGA_C Cuts at: 1936 3151 1936 Size: 1215 4594 SapI GCTCTTCn'nnn_ Cuts at: 2420 2420 Size: 5809 SbfI CC_TGCA'GG Cuts at: 3161 3161 Size: 5809 ScaI AGT'ACT Cuts at: 4057 5769 4057 Size: 1712 4097 SfcI C'TryA_G Cuts at: 826 1045 2107 3157 4292 4973 5164 826 Size: 219 1062 1050 1135 681 191 1471 Fragments arranged by size: 1471 1135 1062 1050 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5809 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5809 SmaI CCC'GGG Cuts at: 1872 3136 1872 Size: 1264 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2887 3913 4814 5076 820 Size: 197 926 944 1026 901 262 1553 Fragments arranged by size: 1553 1026 944 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3600 493 Size: 3107 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5809 SphI G_CATG'C Cuts at: 1132 2441 3167 1132 Size: 1309 726 3774 Fragments arranged by size: 3774 1309 726 SspI AAT'ATT Cuts at: 5 52 3733 5 Size: 47 3681 2081 Fragments arranged by size: 3681 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5809 StyI C'CwwG_G Cuts at: 513 1065 3248 513 Size: 552 2183 3074 Fragments arranged by size: 3074 2183 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2667 3022 3836 3853 4006 4191 4636 Size: 1255 1087 355 814 17 153 185 445 Cuts at: 4636 325 Size: 1498 Fragments arranged by size: 1498 1255 1087 814 445 355 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4055 5767 96 Size: 274 80 33 51 1443 2078 1712 138 Fragments arranged by size: 2078 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2729 2761 5769 5801 2729 Size: 32 3008 32 2737 Fragments arranged by size: 3008 2737 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5809 XbaI T'CTAG_A Cuts at: 3145 3145 Size: 5809 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5809 XmaI C'CCGG_G Cuts at: 1870 3134 1870 Size: 1264 4545 XmnI GAAnn'nnTTC Cuts at: 3938 3938 Size: 5809 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3617 276 Size: 53 83 186 936 2083 2468 Fragments arranged by size: 2468 2083 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI