Restriction map for pFN21AB6539
 (Circular) (Six-base) MAPSORT of: pFN21AB6539.seq  Check: 3626  from: 1  to: 5809



pFN21AB6539  5809 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:41  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3619     278 
   Size:         53      83     186     936    2083    2468

  Fragments arranged by size: 

                2468    2083     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3152    1937 
   Size:       1215    4594
 
Acc65I G'GTAC_C

Cuts at:   3130    3130 
   Size:       5809
 
AclI AA'CG_TT

Cuts at:   1563    3936    4309    1563 
   Size:       2373     373    3063

  Fragments arranged by size: 

                3063    2373     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3872    5256    5524    1632 
   Size:       2240    1384     268    1917

  Fragments arranged by size: 

                2240    1917    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5612
 
AflIII A'CryG_T

Cuts at:   1203    4708    5685    1203 
   Size:       3505     977    1327

  Fragments arranged by size: 

                3505    1327     977
 
AgeI A'CCGG_T

Cuts at:   4640    4640 
   Size:       5809
 
AhdI GACnn_n'nnGTC

Cuts at:   4538    4538 
   Size:       5809
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5809
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5809
 
AlwNI CAG_nnn'CTG

Cuts at:   2781    3552    5124    2781 
   Size:        771    1572    3466

  Fragments arranged by size: 

                3466    1572     771
 
ApaI G_GGCC'C

Cuts at:   2479    2620    2806    2479 
   Size:        141     186    5482

  Fragments arranged by size: 

                5482     186     141
 
ApaLI G'TGCA_C

Cuts at:   3866    5022    3866 
   Size:       1156    4653
 
ApoI r'AATT_y

Cuts at:   1493    3118    3344    3496    5605    1493 
   Size:       1625     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1625     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4363     160 
   Size:        872    3331    1606

  Fragments arranged by size: 

                3331    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5809
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3134    1870 
   Size:         73    1191    4545

  Fragments arranged by size: 

                4545    1191      73
 
BamHI G'GATC_C

Cuts at:   3139    3506    3139 
   Size:        367    5442
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2216    2414    3130    4585     618 
   Size:        325     217     627     429     198     716    1455    1842

  Fragments arranged by size: 

                1842    1455     716     627     429     325     217     198
            
 
BanII G_rGCy'C

Cuts at:   1391    2341    2479    2516    2620    2806    3068    3128    1391 
   Size:        950     138      37     104     186     262      60    4072

  Fragments arranged by size: 

                4072     950     262     186     138     104      60      37
            
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4838
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4000    4034    1903 
   Size:         34    2063      34    3678

  Fragments arranged by size: 

                3678    2063      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2161    3701    4911    2161 
   Size:       1540    1210    3059

  Fragments arranged by size: 

                3059    1540    1210
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5809
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4420     136 
   Size:        107     122      71     631     605    2748    1525

  Fragments arranged by size: 

                2748    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5804    5804 
   Size:       5809
 
BlpI GC'TnA_GC

Cuts at:   3226    3226 
   Size:       5809
 
Bme1580I G_kGCm'C

Cuts at:   1790    2479    2620    2806    3870    5026    1790 
   Size:        689     141     186    1064    1156    2573

  Fragments arranged by size: 

                2573    1156    1064     689     186     141
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5809
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2791    3540    4498 
   Size:        416      53      83     399      24    1374     749     958

Cuts at:   4498     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1374     958     749     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5809
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2419    4469    1239 
   Size:       1180    2050    2579

  Fragments arranged by size: 

                2579    2050    1180
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2872    3934    4799    5097    2872 
   Size:       1062     865     298    3584

  Fragments arranged by size: 

                3584    1062     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4472     882 
   Size:        617    2973    2219

  Fragments arranged by size: 

                2973    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2898    3600     493 
   Size:        711    1694     702    2702

  Fragments arranged by size: 

                2702    1694     711     702
 
BsaBI GATnn'nnATC

Cuts at:   2288    5803    2288 
   Size:       3515    2294
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2196    3616    3998    4633 
   Size:         53      83     186     936     663    1420     382     635

Cuts at:   4633     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1420     936     663     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   2382    4242    4640    4914    5061    5472    2382 
   Size:       1860     398     274     147     411    2719

  Fragments arranged by size: 

                2719    1860     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5584

  Fragments arranged by size: 

                5584     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2443    2744    2747    2768    1186 
   Size:       1257     301       3      21    4227

  Fragments arranged by size: 

                4227    1257     301      21       3
 
BseYI C'CCAG_C

Cuts at:   2472    2613    5012    5508    2472 
   Size:        141    2399     496    2773

  Fragments arranged by size: 

                2773    2399     496     141
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5236
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3259    4020    5048    1540 
   Size:        275     186    1258     761    1028    2301

  Fragments arranged by size: 

                2301    1258    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2375    2516    3870    3955    5026    2375 
   Size:        141    1354      85    1071    3158

  Fragments arranged by size: 

                3158    1354    1071     141      85
 
BsmI GAATG_Cn'

Cuts at:   3320    3413    3320 
   Size:         93    5716
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2341    2375    2479    2516    2620    2806    3068 
   Size:        399     551      34     104      37     104     186     262

Cuts at:   3068    3128    3870    3955    5026    1391 
   Size:         60     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     551     399     262     186     104
                 104      85      60      37      34
 
BspEI T'CCGG_A

Cuts at:   5472    5472 
   Size:       5809
 
BspHI T'CATG_A

Cuts at:   2308    3698    5428    2308 
   Size:       1390    1730    2689

  Fragments arranged by size: 

                2689    1730    1390
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2240    3097    3164     844 
   Size:       1396     857      67    3489

  Fragments arranged by size: 

                3489    1396     857      67
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3696    1394 
   Size:        429    1873    3507

  Fragments arranged by size: 

                3507    1873     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2278    2608    4304    4478      65 
   Size:       1183    1030     330    1696     174    1396

  Fragments arranged by size: 

                1696    1396    1183    1030     330     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4453    4640    1700 
   Size:        110    2643     187    2869

  Fragments arranged by size: 

                2869    2643     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5809
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5809
 
BssSI C'ACGA_G

Cuts at:   2432    3869    4881    2432 
   Size:       1437    1012    3360

  Fragments arranged by size: 

                3360    1437    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5809
 
BstBI TT'CG_AA

Cuts at:   4613    4613 
   Size:       5809
 
BstEII G'GTnAC_C

Cuts at:   1184    2998    1184 
   Size:       1814    3995
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2971    2971 
   Size:       5809
 
BstYI r'GATC_y

Cuts at:   1972    2283    3139    3506    3891    3908    5360    5446    5458 
   Size:        311     856     367     385      17    1452      86      12

Cuts at:   5458    5804    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     856     385     367     346     311      86
                  17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2300    2300 
   Size:       5809
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    5257
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2818     487 
   Size:        683      56    1592    3478

  Fragments arranged by size: 

                3478    1592     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3035    3325    4119    4139    3035 
   Size:        290     794      20    4705

  Fragments arranged by size: 

                4705     794     290      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5809
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5774
 
DraI TTT'AAA

Cuts at:   3113    3113 
   Size:       5809
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2249    2387    4816     809 
   Size:        507     933     138    2429    1802

  Fragments arranged by size: 

                2429    1802     933     507     138
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2740    3256    4145 
   Size:         54    1425      50     275      18     910     516     889

Cuts at:   4145    5774       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     910     889     516     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3256    1537 
   Size:        275    1444    4090

  Fragments arranged by size: 

                4090    1444     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2067    2420    3739    1353 
   Size:        714     353    1319    3423

  Fragments arranged by size: 

                3423    1319     714     353
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4396    4770    4916     387 
   Size:       1064     138    2807     374     146    1280

  Fragments arranged by size: 

                2807    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2514    2514 
   Size:       5809
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2419    3872    4469    5256    5524    1239 
   Size:        393     787    1453     597     787     268    1524

  Fragments arranged by size: 

                1524    1453     787     787     597     393     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:   3008    3008 
   Size:       5809
 
EcoO109I rG'GnC_Cy

Cuts at:   2315    2476    2617    2803    2315 
   Size:        161     141     186    5321

  Fragments arranged by size: 

                5321     186     161     141
 
EcoRI G'AATT_C

Cuts at:   3118    3496    3118 
   Size:        378    5431
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2114    2114 
   Size:       5809
 
FspI TGC'GCA

Cuts at:   2270    4315    2270 
   Size:       2045    3764
 
HaeII r_GCGC'y

Cuts at:   1678    4956    1678 
   Size:       3278    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2268    2300    2639    2671    2870 
   Size:         32     135      32     696      32     339      32     199

Cuts at:   2870    2902    4450    4482    4524    4556    1373 
   Size:         32    1548      32      42      32    2626

  Fragments arranged by size: 

                2626    1548     696     339     199     135      42      32
                  32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3052    3153    3399     669 
   Size:        988     281    1114     101     246    3079

  Fragments arranged by size: 

                3079    1114     988     281     246     101
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5809
 
HpaI GTT'AAC

Cuts at:   3399    3399 
   Size:       5809
 
KpnI G_GTAC'C

Cuts at:   3134    3134 
   Size:       5809
 
MfeI C'AATT_G

Cuts at:   3408    3408 
   Size:       5809
 
MluI A'CGCG_T

Cuts at:   5685    5685 
   Size:       5809
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4923    5107    1305 
   Size:       3618     184    2007

  Fragments arranged by size: 

                3618    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3987

  Fragments arranged by size: 

                3987    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2436    3767    4126    4285     518 
   Size:        648    1270    1331     359     159    2042

  Fragments arranged by size: 

                2042    1331    1270     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2613    2841    3225    3902    5050    5295    1606 
   Size:       1007     228     384     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1007     677     384     245     228
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5257
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5809
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5809
 
NotI GC'GGCC_GC

Cuts at:   3256    3256 
   Size:       5809
 
NspI r_CATG'y

Cuts at:   1132    1229    2441    2945    3167    4712    1132 
   Size:         97    1212     504     222    1545    2229

  Fragments arranged by size: 

                2229    1545    1212     504     222      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5809
 
PciI A'CATG_T

Cuts at:   4708    4708 
   Size:       5809
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5809
 
PmeI GTTT'AAAC

Cuts at:   3113    3113 
   Size:       5809
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3878    3910    5391    5423    3878 
   Size:         32    1481      32    4264

  Fragments arranged by size: 

                4264    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2315    2315 
   Size:       5809
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5809
 
PsiI TTA'TAA

Cuts at:   3379    3379 
   Size:       5809
 
PspOMI G'GGCC_C

Cuts at:   2475    2616    2802    2475 
   Size:        141     186    5482

  Fragments arranged by size: 

                5482     186     141
 
PstI C_TGCA'G

Cuts at:    830    3161     830 
   Size:       2331    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5809
 
PvuII CAG'CTG

Cuts at:   2613    2841    2613 
   Size:        228    5581
 
SacI G_AGCT'C

Cuts at:   2516    2516 
   Size:       5809
 
SalI G'TCGA_C

Cuts at:   1936    3151    1936 
   Size:       1215    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2420    2420 
   Size:       5809
 
SbfI CC_TGCA'GG

Cuts at:   3161    3161 
   Size:       5809
 
ScaI AGT'ACT

Cuts at:   4057    5769    4057 
   Size:       1712    4097
 
SfcI C'TryA_G

Cuts at:    826    1045    2107    3157    4292    4973    5164     826 
   Size:        219    1062    1050    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1062    1050     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5809
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5809
 
SmaI CCC'GGG

Cuts at:   1872    3136    1872 
   Size:       1264    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2887    3913    4814    5076     820 
   Size:        197     926     944    1026     901     262    1553

  Fragments arranged by size: 

                1553    1026     944     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3600     493 
   Size:       3107    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5809
 
SphI G_CATG'C

Cuts at:   1132    2441    3167    1132 
   Size:       1309     726    3774

  Fragments arranged by size: 

                3774    1309     726
 
SspI AAT'ATT

Cuts at:      5      52    3733       5 
   Size:         47    3681    2081

  Fragments arranged by size: 

                3681    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5809
 
StyI C'CwwG_G

Cuts at:    513    1065    3248     513 
   Size:        552    2183    3074

  Fragments arranged by size: 

                3074    2183     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2667    3022    3836    3853    4006    4191    4636 
   Size:       1255    1087     355     814      17     153     185     445

Cuts at:   4636     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1255    1087     814     445     355     185     153
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4055    5767      96 
   Size:        274      80      33      51    1443    2078    1712     138

  Fragments arranged by size: 

                2078    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2729    2761    5769    5801    2729 
   Size:         32    3008      32    2737

  Fragments arranged by size: 

                3008    2737      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5809
 
XbaI T'CTAG_A

Cuts at:   3145    3145 
   Size:       5809
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5809
 
XmaI C'CCGG_G

Cuts at:   1870    3134    1870 
   Size:       1264    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3938    3938 
   Size:       5809
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3617     276 
   Size:         53      83     186     936    2083    2468

  Fragments arranged by size: 

                2468    2083     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI
     NspI     PasI     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI
     PsiI   PspOMI     PstI     PvuI    PvuII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI  BstZ17I   DraIII    EcoRV
     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     NsiI
     PacI     PfoI     PmlI    PspXI     PsrI    RsrII    SacII    SanDI
    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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