Restriction map for
pFN21AB6469
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# NO CUT ENZYMES ( 150 enzymes) #
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AccI AccII AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspHI AspI AvaI AviII BalI
BamHI BanII BbeI BcgI BciVI
BclI BfrI BglI BglII BlpI
BpmI Bpu1102I BsaBI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI HinP1I HincII HindII HpaI
HphI KasI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
SspBI SspI SwaI Tth111I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 694 GACGTC GACGT^C
Acc65I 281 GGTACC G^GTACC
AciI 567 GCGG CCGC(-3/-1)
AcyI 694 GACGTC G[GA]^CG[CT]C
Asp718I 281 GGTACC G^GTACC
AvrII 390 CCTAGG C^CTAGG
BanI 281 GGTACC G^G[CT][GA]CC
BbrPI 469 CACGTG CAC^GTG
BbvCI 499 GCTGAGG CCTCAGC(-5/-2)
BcnI 846 CCGGG CC^[GC]GG
BfaI 391 CTAG C^TAG
BlnI 390 CCTAGG C^CTAGG
BmyI 552 GGGCAC G[AGT]GC[ACT]^C
BsaAI 469 CACGTG [CT]AC^GT[GA]
BsaHI 694 GACGTC G[GA]^CG[CT]C
BseRI 952 GAGGAG GAGGAG(10/8)
Bsp1286I 552 GGGCAC G[AGT]GC[ACT]^C
BspHI 332 TCATGA T^CATGA
BspLU11I 230 ACATGT A^CATGT
BspMI 704 ACCTGC ACCTGC(4/8)
BsrDI 315 CATTGC GCAATG(2/0)
BsrI 476 CCAGT ACTGG(1/-1)
BstF5I 849 GGATG GGATG(2/0)
EarI 880 GAAGAG CTCTTC(1/4)
FokI 849 GGATG GGATG(9/13)
Hin1I 694 GACGTC G[GA]^CG[CT]C
HindIII 610 AAGCTT A^AGCTT
KpnI 281 GGTACC GGTAC^C
Ksp632I 880 GAAGAG CTCTTC(1/4)
MaeI 391 CTAG C^TAG
MseI 245 TTAA T^TAA
NciI 846 CCGGG CC^[GC]GG
NcoI 585 CCATGG C^CATGG
NlaIV 281 GGTACC GG[ACGT]^[ACGT]CC
NspI 230 ACATGT [GA]CATG^[CT]
PmaCI 469 CACGTG CAC^GTG
PmlI 469 CACGTG CAC^GTG
PpuMI 386 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 685 CAGCTG CAG^CTG
RcaI 332 TCATGA T^CATGA
SbfI 723 CCTGCAGG CCTGCA^GG
ScaI 138 AGTACT AGT^ACT
SexAI 578 ACCAGGT A^CC[AT]GGT
SfaNI 850 GATGC GCATC(5/9)
Sse8387I 723 CCTGCAGG CCTGCA^GG
StuI 740 AGGCCT AGG^CCT
Tru9I 245 TTAA T^TAA
Tsp45I 472 GTGAC ^GT[GC]AC
XcmI 758 CCAATGACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AflIII 230 ACATGT A^C[GA][CT]GT
468 ACACGT
BbsI 753 GAAGAC GAAGAC(2/6)
697 GTCTTC
BpuAI 753 GAAGAC GAAGAC(2/6)
697 GTCTTC
BsgI 814 CTGCAC GTGCAG(16/14)
16 GTGCAG
BsmFI 597 GGGAC GGGAC(10/14)
561 GTCCC
DdeI 49 CTCAG C^T[ACGT]AG
500 CTGAG
DraII 291 AGGCCCT [GA]G^G[ACGT]CC[CT]
386 AGGACCT
DsaI 585 CCATGG C^C[GA][CT]GG
803 CCGTGG
EcoO109I 291 AGGCCCT [GA]G^G[ACGT]CC[CT]
386 AGGACCT
EcoRV 532 GATATC GAT^ATC
619 GATATC
EcoT14I 390 CCTAGG C^C[AT][AT]GG
585 CCATGG
HapII 4 CCGG C^CGG
846 CCGG
HinfI 74 GAATC G^A[ACGT]TC
193 GATTC
HpaII 4 CCGG C^CGG
846 CCGG
MaeII 470 ACGT A^CGT
695 ACGT
MaeIII 472 GTGAC ^GT[ACGT]AC
525 GTTAC
MspA1I 565 CAGCGG C[AC]G^C[GT]G
685 CAGCTG
MspI 4 CCGG C^CGG
846 CCGG
MwoI 9 GCTGGCAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
490 GCAGAGGAAGC
PflMI 33 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
765 CCAGATGGTGG
PstI 688 CTGCAG CTGCA^G
724 CTGCAG
StyI 390 CCTAGG C^C[AT][AT]GG
585 CCATGG
TaqI 151 TCGA T^CGA
454 TCGA
TfiI 74 GAATC G^A[AT]TC
193 GATTC
Tsp509I 352 AATT ^AATT
483 AATT
TthHB8I 151 TCGA T^CGA
454 TCGA
Van91I 33 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
765 CCAGATGGTGG
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AfaI AluI AlwI AvaII BbvI
BsaJI BsiYI BslI BssKI BstNI
BstXI Cac8I Cfr13I DpnII EcoRII
Fnu4HI HaeIII ItaI MboI MboII
MnlI MslI MvaI NdeII NlaIII
RsaI Sau3AI Sau96I ScrFI SfcI
TseI TspRI