(Circular) (Six-base) MAPSORT of: pFN21AB6469.seq Check: 3197 from: 1 to: 5677 pFN21AB6469 5677 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2703 3487 278 Size: 53 83 186 936 1167 784 2468 Fragments arranged by size: 2468 1167 936 784 186 83 53 AccI GT'mk_AC Cuts at: 1937 3020 1937 Size: 1083 4594 Acc65I G'GTAC_C Cuts at: 2286 2998 2286 Size: 712 4965 AclI AA'CG_TT Cuts at: 1563 3804 4177 1563 Size: 2241 373 3063 Fragments arranged by size: 3063 2241 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2777 3740 5124 5392 1632 Size: 1145 963 1384 268 1917 Fragments arranged by size: 1917 1384 1145 963 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5480 AflIII A'CryG_T Cuts at: 1203 2235 2473 4576 5553 1203 Size: 1032 238 2103 977 1327 Fragments arranged by size: 2103 1327 1032 977 238 AgeI A'CCGG_T Cuts at: 4508 4508 Size: 5677 AhdI GACnn_n'nnGTC Cuts at: 4406 4406 Size: 5677 AleI CACnn'nnGTG Cuts at: 1166 2552 1166 Size: 1386 4291 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5677 AlwNI CAG_nnn'CTG Cuts at: 3420 4992 3420 Size: 1572 4105 ApaLI G'TGCA_C Cuts at: 3734 4890 3734 Size: 1156 4521 ApoI r'AATT_y Cuts at: 1493 2986 3212 3364 5473 1493 Size: 1493 226 152 2109 1697 Fragments arranged by size: 2109 1697 1493 226 152 AseI AT'TA_AT Cuts at: 160 1032 4231 160 Size: 872 3199 1606 Fragments arranged by size: 3199 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 AvaI C'yCGr_G Cuts at: 1870 1943 3002 1870 Size: 73 1059 4545 Fragments arranged by size: 4545 1059 73 AvrII C'CTAG_G Cuts at: 2395 2395 Size: 5677 BamHI G'GATC_C Cuts at: 3007 3374 3007 Size: 367 5310 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2286 2998 4453 618 Size: 325 217 627 499 712 1455 1842 Fragments arranged by size: 1842 1455 712 627 499 325 217 BanII G_rGCy'C Cuts at: 1391 2996 1391 Size: 1605 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2695 2765 928 Size: 971 796 70 3840 Fragments arranged by size: 3840 971 796 70 BbvCI CC'TCA_GC Cuts at: 2505 2505 Size: 5677 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3868 3902 1903 Size: 34 50 34 1847 34 3678 Fragments arranged by size: 3678 1847 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3569 4779 3569 Size: 1210 4467 BclI T'GATC_A Cuts at: 1548 1548 Size: 5677 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4288 136 Size: 107 122 71 631 605 2616 1525 Fragments arranged by size: 2616 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5672 5672 Size: 5677 BlpI GC'TnA_GC Cuts at: 3094 3094 Size: 5677 Bme1580I G_kGCm'C Cuts at: 1790 2561 3738 4894 1790 Size: 771 1177 1156 2573 Fragments arranged by size: 2573 1177 1156 771 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5677 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3408 4366 442 Size: 416 53 83 399 24 1991 958 1753 Fragments arranged by size: 1991 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5677 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4337 1239 Size: 3098 2579 Bpu10I CC'TnA_GC Cuts at: 2505 2505 Size: 5677 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3802 4667 4965 3802 Size: 865 298 4514 Fragments arranged by size: 4514 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4340 882 Size: 617 2841 2219 Fragments arranged by size: 2841 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2476 3468 493 Size: 711 1272 992 2702 Fragments arranged by size: 2702 1272 992 711 BsaBI GATnn'nnATC Cuts at: 5671 5671 Size: 5677 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2700 3484 3866 4501 Size: 53 83 186 936 1167 784 382 635 Cuts at: 4501 275 Size: 1451 Fragments arranged by size: 1451 1167 936 784 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4110 4508 4782 4929 5340 4110 Size: 398 274 147 411 4447 Fragments arranged by size: 4447 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5452 Fragments arranged by size: 5452 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2972 1186 Size: 1786 3891 BseYI C'CCAG_C Cuts at: 2568 4880 5376 2568 Size: 2312 496 2869 Fragments arranged by size: 2869 2312 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2042 2804 1163 Size: 573 306 762 4036 Fragments arranged by size: 4036 762 573 306 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3127 3888 4916 1540 Size: 275 186 1126 761 1028 2301 Fragments arranged by size: 2301 1126 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3738 3823 4894 3738 Size: 85 1071 4521 Fragments arranged by size: 4521 1071 85 BsmI GAATG_Cn' Cuts at: 3188 3281 3188 Size: 93 5584 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2561 2996 3738 3823 4894 1391 Size: 399 771 435 742 85 1071 2174 Fragments arranged by size: 2174 1071 771 742 435 399 85 BspEI T'CCGG_A Cuts at: 5340 5340 Size: 5677 BspHI T'CATG_A Cuts at: 2337 3566 5296 2337 Size: 1229 1730 2718 Fragments arranged by size: 2718 1730 1229 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2718 3032 844 Size: 1874 314 3489 Fragments arranged by size: 3489 1874 314 BsrBI CCG'CTC Cuts at: 1394 1823 3564 1394 Size: 429 1741 3507 Fragments arranged by size: 3507 1741 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2319 4172 4346 65 Size: 1183 1071 1853 174 1396 Fragments arranged by size: 1853 1396 1183 1071 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4321 4508 1700 Size: 110 2511 187 2869 Fragments arranged by size: 2869 2511 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5677 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5677 BssSI C'ACGA_G Cuts at: 3737 4749 3737 Size: 1012 4665 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5677 BstBI TT'CG_AA Cuts at: 4481 4481 Size: 5677 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5677 BstXI CCAn_nnnn'nTGG Cuts at: 2444 2591 2620 2444 Size: 147 29 5501 Fragments arranged by size: 5501 147 29 BstYI r'GATC_y Cuts at: 1972 3007 3374 3759 3776 5228 5314 5326 5672 Size: 1035 367 385 17 1452 86 12 346 Cuts at: 5672 1972 Size: 1977 Fragments arranged by size: 1977 1452 1035 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2590 2808 513 Size: 552 1525 218 3382 Fragments arranged by size: 3382 1525 552 218 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4938 Fragments arranged by size: 4938 683 56 BtsI GCAGTG_nn' Cuts at: 2025 3193 3987 4007 2025 Size: 1168 794 20 3695 Fragments arranged by size: 3695 1168 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5677 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5642 DraI TTT'AAA Cuts at: 2981 2981 Size: 5677 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4684 809 Size: 507 3368 1802 Fragments arranged by size: 3368 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3124 4013 5642 Size: 54 1425 50 275 18 1294 889 1629 Cuts at: 5642 8 Size: 43 Fragments arranged by size: 1629 1425 1294 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3124 1537 Size: 275 1312 4090 Fragments arranged by size: 4090 1312 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2880 3607 1353 Size: 1527 727 3423 Fragments arranged by size: 3423 1527 727 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4264 4638 4784 387 Size: 1064 138 2675 374 146 1280 Fragments arranged by size: 2675 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2777 3740 4337 5124 5392 1239 Size: 393 1145 963 597 787 268 1524 Fragments arranged by size: 1524 1145 963 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2297 2392 2297 Size: 95 5582 EcoRI G'AATT_C Cuts at: 2986 3364 2986 Size: 378 5299 EcoRV GAT'ATC Cuts at: 2539 2626 2539 Size: 87 5590 FspI TGC'GCA Cuts at: 4183 4183 Size: 5677 HaeII r_GCGC'y Cuts at: 1678 4824 1678 Size: 3146 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4318 4350 4392 4424 1373 Size: 32 135 32 2746 32 42 32 2626 Fragments arranged by size: 2746 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3021 3267 669 Size: 988 281 1083 246 3079 Fragments arranged by size: 3079 1083 988 281 246 HindIII A'AGCT_T Cuts at: 748 2615 748 Size: 1867 3810 HpaI GTT'AAC Cuts at: 3267 3267 Size: 5677 KpnI G_GTAC'C Cuts at: 2290 3002 2290 Size: 712 4965 MfeI C'AATT_G Cuts at: 3276 3276 Size: 5677 MluI A'CGCG_T Cuts at: 5553 5553 Size: 5677 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2071 2477 2494 4791 4975 1305 Size: 766 406 17 2297 184 2007 Fragments arranged by size: 2297 2007 766 406 184 17 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3855 Fragments arranged by size: 3855 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2313 2378 2423 2442 2552 3635 3994 Size: 648 1147 65 45 19 110 1083 359 Cuts at: 3994 4153 518 Size: 159 2042 Fragments arranged by size: 2042 1147 1083 648 359 159 110 65 45 19 MspA1I CmG'CkG Cuts at: 1606 2572 2692 3093 3770 4918 5163 1606 Size: 966 120 401 677 1148 245 2120 Fragments arranged by size: 2120 1148 966 677 401 245 120 NcoI C'CATG_G Cuts at: 513 1065 2590 513 Size: 552 1525 3600 Fragments arranged by size: 3600 1525 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5677 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5677 NotI GC'GGCC_GC Cuts at: 3124 3124 Size: 5677 NspI r_CATG'y Cuts at: 1132 1229 2239 3035 4580 1132 Size: 97 1010 796 1545 2229 Fragments arranged by size: 2229 1545 1010 796 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5677 PciI A'CATG_T Cuts at: 2235 4576 2235 Size: 2341 3336 PflMI CCAn_nnn'nTGG Cuts at: 1108 2044 2776 1108 Size: 936 732 4009 Fragments arranged by size: 4009 936 732 PmeI GTTT'AAAC Cuts at: 2981 2981 Size: 5677 PmlI CAC'GTG Cuts at: 2476 2476 Size: 5677 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3746 3778 5259 5291 3746 Size: 32 1481 32 4132 Fragments arranged by size: 4132 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2392 2392 Size: 5677 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5677 PsiI TTA'TAA Cuts at: 3247 3247 Size: 5677 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2514 2546 2514 Size: 32 5645 PstI C_TGCA'G Cuts at: 830 2697 2733 3029 830 Size: 1867 36 296 3478 Fragments arranged by size: 3478 1867 296 36 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5677 PvuII CAG'CTG Cuts at: 2692 2692 Size: 5677 SalI G'TCGA_C Cuts at: 1936 3019 1936 Size: 1083 4594 SbfI CC_TGCA'GG Cuts at: 2733 3029 2733 Size: 296 5381 ScaI AGT'ACT Cuts at: 2145 3925 5637 2145 Size: 1780 1712 2185 Fragments arranged by size: 2185 1780 1712 SexAI A'CCwGG_T Cuts at: 2583 2583 Size: 5677 SfcI C'TryA_G Cuts at: 826 1045 2406 2693 2729 3025 4160 4841 5032 Size: 219 1361 287 36 296 1135 681 191 Cuts at: 5032 826 Size: 1471 Fragments arranged by size: 1471 1361 1135 681 296 287 219 191 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5677 SmaI CCC'GGG Cuts at: 1872 3004 1872 Size: 1132 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3781 4682 4944 820 Size: 197 926 1838 901 262 1553 Fragments arranged by size: 1838 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3468 493 Size: 2975 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5677 SphI G_CATG'C Cuts at: 1132 3035 1132 Size: 1903 3774 SspI AAT'ATT Cuts at: 5 52 3601 5 Size: 47 3549 2081 Fragments arranged by size: 3549 2081 47 StuI AGG'CCT Cuts at: 1517 2747 1517 Size: 1230 4447 StyI C'CwwG_G Cuts at: 513 1065 2395 2590 3116 513 Size: 552 1330 195 526 3074 Fragments arranged by size: 3074 1330 552 526 195 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3704 3721 3874 4059 4504 325 Size: 1255 2124 17 153 185 445 1498 Fragments arranged by size: 2124 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2143 2784 3923 Size: 274 80 33 51 1443 166 641 1139 Cuts at: 3923 5635 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1139 641 274 166 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5637 5669 5637 Size: 32 5645 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5677 XbaI T'CTAG_A Cuts at: 3013 3013 Size: 5677 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2770 2011 Size: 759 4918 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5677 XmaI C'CCGG_G Cuts at: 1870 3002 1870 Size: 1132 4545 XmnI GAAnn'nnTTC Cuts at: 3806 3806 Size: 5677 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2701 3485 276 Size: 53 83 186 936 1167 784 2468 Fragments arranged by size: 2468 1167 936 784 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI