Restriction map for
pFN21AB6438
(Linear) (Six-base) MAPSORT of: KIBB6438.seq Check: 1083 from: 1 to: 1020
KIBB6438.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 271 647 1020
Size: 271 376 373
Fragments arranged by size:
376 373 271
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 868 870 1020
Size: 523 345 2 150
Fragments arranged by size:
523 345 150 2
AflIII A'CryG_T
Cuts at: 0 185 1020
Size: 185 835
AleI CACnn'nnGTG
Cuts at: 0 352 1020
Size: 352 668
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 253 1020
Size: 253 767
AlwNI CAG_nnn'CTG
Cuts at: 0 795 957 1020
Size: 795 162 63
Fragments arranged by size:
795 162 63
ApaI G_GGCC'C
Cuts at: 0 176 311 574 821 1020
Size: 176 135 263 247 199
Fragments arranged by size:
263 247 199 176 135
AvaI C'yCGr_G
Cuts at: 0 311 1020
Size: 311 709
BanI G'GyrC_C
Cuts at: 0 39 109 596 1020
Size: 39 70 487 424
Fragments arranged by size:
487 424 70 39
BanII G_rGCy'C
Cuts at: 0 20 176 311 574 821 1020
Size: 20 156 135 263 247 199
Fragments arranged by size:
263 247 199 156 135 20
BbeI G_GCGC'C
Cuts at: 0 600 1020
Size: 600 420
BciVI GTATCCnnnnn_n'
Cuts at: 0 928 1020
Size: 928 92
BglI GCCn_nnn'nGGC
Cuts at: 0 171 798 1020
Size: 171 627 222
Fragments arranged by size:
627 222 171
BlpI GC'TnA_GC
Cuts at: 0 743 1020
Size: 743 277
Bme1580I G_kGCm'C
Cuts at: 0 112 176 311 530 574 586 666 821
Size: 112 64 135 219 44 12 80 155
Cuts at: 821 1020
Size: 199
Fragments arranged by size:
219 199 155 135 112 80 64 44
12
BmrI ACTGGGnnnn_n'
Cuts at: 0 815 885 1020
Size: 815 70 135
Fragments arranged by size:
815 135 70
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 516 1020
Size: 97 419 504
Fragments arranged by size:
504 419 97
Bpu10I CC'TnA_GC
Cuts at: 0 14 835 1020
Size: 14 821 185
Fragments arranged by size:
821 185 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 1020
Size: 377 643
BsaAI yAC'GTr
Cuts at: 0 188 667 916 1020
Size: 188 479 249 104
Fragments arranged by size:
479 249 188 104
BsaHI Gr'CG_yC
Cuts at: 0 221 597 881 1020
Size: 221 376 284 139
Fragments arranged by size:
376 284 221 139
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 946 949 952 1020
Size: 946 3 3 68
Fragments arranged by size:
946 68 3 3
BseYI C'CCAG_C
Cuts at: 0 46 55 323 1020
Size: 46 9 268 697
Fragments arranged by size:
697 268 46 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 985 1020
Size: 985 35
BsiHKAI G_wGCw'C
Cuts at: 0 20 944 1020
Size: 20 924 76
Fragments arranged by size:
924 76 20
Bsp1286I G_dGCh'C
Cuts at: 0 20 112 176 311 530 574 586 666
Size: 20 92 64 135 219 44 12 80
Cuts at: 666 821 944 1020
Size: 155 123 76
Fragments arranged by size:
219 155 135 123 92 80 76 64
44 20 12
BspHI T'CATG_A
Cuts at: 0 452 1020
Size: 452 568
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 271 623 647 1020
Size: 271 352 24 373
Fragments arranged by size:
373 352 271 24
BsrBI CCG'CTC
Cuts at: 0 342 1020
Size: 342 678
BsrDI GCAATG_nn'
Cuts at: 0 249 1020
Size: 249 771
BssHII G'CGCG_C
Cuts at: 0 51 245 281 738 1020
Size: 51 194 36 457 282
Fragments arranged by size:
457 282 194 51 36
BstAPI GCAn_nnn'nTGC
Cuts at: 0 116 795 1020
Size: 116 679 225
Fragments arranged by size:
679 225 116
BstYI r'GATC_y
Cuts at: 0 953 986 1020
Size: 953 33 34
Fragments arranged by size:
953 34 33
BtgI C'CryG_G
Cuts at: 0 147 167 332 1020
Size: 147 20 165 688
Fragments arranged by size:
688 165 147 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 84 1020
Size: 84 936
BtsI GCAGTG_nn'
Cuts at: 0 247 1020
Size: 247 773
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 533 568 1020
Size: 533 35 452
Fragments arranged by size:
533 452 35
DraIII CAC_nnn'GTG
Cuts at: 0 116 191 1020
Size: 116 75 829
Fragments arranged by size:
829 116 75
EaeI y'GGCC_r
Cuts at: 0 144 150 753 1020
Size: 144 6 603 267
Fragments arranged by size:
603 267 144 6
EarI CTCTTCn'nnn_
Cuts at: 0 454 1020
Size: 454 566
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 937 1020
Size: 937 83
EcoICRI GAG'CTC
Cuts at: 0 18 1020
Size: 18 1002
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 516 523 868 870 1020
Size: 97 419 7 345 2 150
Fragments arranged by size:
419 345 150 97 7 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 619 1020
Size: 619 401
EcoO109I rG'GnC_Cy
Cuts at: 0 172 308 315 570 681 817 831 996
Size: 172 136 7 255 111 136 14 165
Cuts at: 996 1020
Size: 24
Fragments arranged by size:
255 172 165 136 136 111 24 14
7
FspI TGC'GCA
Cuts at: 0 97 229 1020
Size: 97 132 791
Fragments arranged by size:
791 132 97
HaeII r_GCGC'y
Cuts at: 0 600 1020
Size: 600 420
KasI G'GCGC_C
Cuts at: 0 596 1020
Size: 596 424
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 758 1020
Size: 758 262
MscI TGG'CCA
Cuts at: 0 152 1020
Size: 152 868
MslI CAynn'nnrTG
Cuts at: 0 352 641 1020
Size: 352 289 379
Fragments arranged by size:
379 352 289
MspA1I CmG'CkG
Cuts at: 0 46 135 169 275 334 501 927 1020
Size: 46 89 34 106 59 167 426 93
Fragments arranged by size:
426 167 106 93 89 59 46 34
NarI GG'CG_CC
Cuts at: 0 597 1020
Size: 597 423
NspI r_CATG'y
Cuts at: 0 646 894 1020
Size: 646 248 126
Fragments arranged by size:
646 248 126
PmlI CAC'GTG
Cuts at: 0 188 667 1020
Size: 188 479 353
Fragments arranged by size:
479 353 188
PpuMI rG'GwC_Cy
Cuts at: 0 315 1020
Size: 315 705
PspOMI G'GGCC_C
Cuts at: 0 172 307 570 817 1020
Size: 172 135 263 247 203
Fragments arranged by size:
263 247 203 172 135
PstI C_TGCA'G
Cuts at: 0 242 926 962 1020
Size: 242 684 36 58
Fragments arranged by size:
684 242 58 36
PvuII CAG'CTG
Cuts at: 0 135 501 1020
Size: 135 366 519
Fragments arranged by size:
519 366 135
SacI G_AGCT'C
Cuts at: 0 20 1020
Size: 20 1000
SacII CC_GC'GG
Cuts at: 0 170 335 1020
Size: 170 165 685
Fragments arranged by size:
685 170 165
ScaI AGT'ACT
Cuts at: 0 967 1020
Size: 967 53
SfcI C'TryA_G
Cuts at: 0 238 922 958 1020
Size: 238 684 36 62
Fragments arranged by size:
684 238 62 36
SfoI GGC'GCC
Cuts at: 0 598 1020
Size: 598 422
SmaI CCC'GGG
Cuts at: 0 313 1020
Size: 313 707
SmlI C'TyrA_G
Cuts at: 0 392 1020
Size: 392 628
SnaBI TAC'GTA
Cuts at: 0 916 1020
Size: 916 104
SphI G_CATG'C
Cuts at: 0 894 1020
Size: 894 126
StuI AGG'CCT
Cuts at: 0 236 1020
Size: 236 784
StyI C'CwwG_G
Cuts at: 0 620 1014 1020
Size: 620 394 6
Fragments arranged by size:
620 394 6
TatI w'GTAC_w
Cuts at: 0 422 761 965 1020
Size: 422 339 204 55
Fragments arranged by size:
422 339 204 55
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 132 1020
Size: 132 888
XmaI C'CCGG_G
Cuts at: 0 311 1020
Size: 311 709
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AlwNI ApaI AvaI
BanI BanII BbeI BciVI BglI BlpI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaAI BsaHI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BssHII BstAPI
BstYI BtgI BtgZI BtsI CspCI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII KasI
MmeI MscI MslI MspA1I NarI NspI PmlI PpuMI
PspOMI PstI PvuII SacI SacII ScaI SfcI SfoI
SmaI SmlI SnaBI SphI StuI StyI TatI XcmI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BcgI BclI BfrBI BglII BmgBI
BmtI BplI BsaI BsaBI BsaWI BsaXI BsiEI BsiWI
BsmI BsmBI BspEI BsrFI BsrGI BssSI BstBI BstEII
BstXI BstZ17I Bsu36I ClaI DraI DrdI EagI EcoRI
EcoRV FalI FseI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI
SpeI SrfI SspI SwaI TaqII TstI Tth111I XbaI
XhoI XmnI ZraI