Restriction map for
pFN21AB6405
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# NO CUT ENZYMES ( 147 enzymes) #
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Acc65I AclI AcsI AfaI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbsI BciVI BclI
BcnI BfrI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspLU11I BspMI BsrDI
BsrFI BsrGI BssHII BssSI BstBI
BstEII BstPI Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HgaI HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MspA1I MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsaI RsrII SacII SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 733 GACGTC GACGT^C
AccII 777 CGCG CG^CG
AccIII 80 TCCGGA T^CCGGA
AcyI 733 GACGTC G[GA]^CG[CT]C
AlwI 49 GATCC GGATC(4/5)
Asp700I 292 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 399 GGTCC G^G[AT]CC
BalI 491 TGGCCA TGG^CCA
BcgI 819 CGACTACACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 535 CTAG C^TAG
BglI 421 GCCGTGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 733 GACGTC G[GA]^CG[CT]C
BsaWI 80 TCCGGA [AT]^CCGG[AT]
BseAI 80 TCCGGA T^CCGGA
BspEI 80 TCCGGA T^CCGGA
BspHI 275 TCATGA T^CATGA
BsrBI 283 GAGCGG CCGCTC(-3/-3)
Bst1107I 448 GTATAC GTA^TAC
BstAPI 67 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 777 CGCG CG^CG
BstXI 635 CCAACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 448 GTATAC GTA^TAC
Cac8I 866 GCGTGC GC[ACGT]^[ACGT]GC
CfoI 778 GCGC GCG^C
Cfr13I 399 GGTCC G^G[ACGT]CC
EcoNI 795 CCTCAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HhaI 778 GCGC GCG^C
Hin1I 733 GACGTC G[GA]^CG[CT]C
HinP1I 778 GCGC G^CGC
HincII 817 GTCGAC GT[CT]^[GA]AC
HindII 817 GTCGAC GT[CT]^[GA]AC
HphI 146 TCACC GGTGA(8/7)
MaeI 535 CTAG C^TAG
MluNI 491 TGGCCA TGG^CCA
MroI 80 TCCGGA T^CCGGA
MscI 491 TGGCCA TGG^CCA
MslI 525 CACTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 777 CGCG CG^CG
NlaIV 631 GGAACC GG[ACGT]^[ACGT]CC
RcaI 275 TCATGA T^CATGA
SalI 817 GTCGAC G^TCGAC
SapI 210 GCTCTTC GCTCTTC(1/4)
Sau96I 399 GGTCC G^G[ACGT]CC
SmlI 769 CTCAAG C^T[CT][GA]AG
SspI 372 AATATT AAT^ATT
TaqI 818 TCGA T^CGA
TfiI 141 GAATC G^A[AT]TC
TthHB8I 818 TCGA T^CGA
XmnI 292 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccI 448 GTATAC GT^[AC][GT]AC
817 GTCGAC
AlwNI 68 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
127 CAGGGTCTG
AspHI 208 GAGCTC G[AT]GC[AT]^C
895 GAGCTC
BanII 208 GAGCTC G[GA]GC[CT]^C
895 GAGCTC
BbvCI 62 CCTCAGC CCTCAGC(-5/-2)
878 CCTCAGC
BglII 336 AGATCT A^GATCT
570 AGATCT
BmyI 208 GAGCTC G[AGT]GC[ACT]^C
895 GAGCTC
BpmI 390 CTCCAG CTGGAG(16/14)
91 CTGGAG
BseRI 180 CTCCTC GAGGAG(10/8)
802 GAGGAG
BsiHKAI 208 GAGCTC G[AT]GC[AT]^C
895 GAGCTC
BsiYI 197 CCTCTGGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
795 CCTCAAAGAGG
BslI 197 CCTCTGGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
795 CCTCAAAGAGG
BsmAI 118 GAGAC GTCTC(1/5)
33 GTCTC
BsmFI 517 GTCCC GGGAC(10/14)
736 GTCCC
Bsp1286I 208 GAGCTC G[AGT]GC[ACT]^C
895 GAGCTC
BsrI 784 ACTGG ACTGG(1/-1)
149 CCAGT
BstF5I 78 CATCC GGATG(2/0)
808 CATCC
DsaI 422 CCGTGG C^C[GA][CT]GG
811 CCGTGG
EaeI 428 CGGCCA [CT]^GGCC[GA]
491 TGGCCA
EarI 211 CTCTTC CTCTTC(1/4)
102 GAAGAG
FauI 203 GCGGG CCCGC(4/6)
285 GCGGG
FokI 78 CATCC GGATG(9/13)
808 CATCC
HapII 81 CCGG C^CGG
708 CCGG
HpaII 81 CCGG C^CGG
708 CCGG
Ksp632I 211 CTCTTC CTCTTC(1/4)
102 GAAGAG
MspI 81 CCGG C^CGG
708 CCGG
SacI 208 GAGCTC GAGCT^C
895 GAGCTC
SfaNI 538 GATGC GCATC(5/9)
807 GCATC
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI BbvI BsaJI BssKI
BstNI BstYI DdeI DpnII EcoRII
Fnu4HI HaeIII HinfI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MseI MvaI MwoI NdeII NlaIII
PleI Sau3AI ScrFI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII