(Circular) (Six-base) MAPSORT of: pFN21AB6405.seq Check: 9489 from: 1 to: 5620 pFN21AB6405 5620 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2742 3430 278 Size: 53 83 186 936 1206 688 2468 Fragments arranged by size: 2468 1206 936 688 186 83 53 AccI GT'mk_AC Cuts at: 1937 2454 2823 2963 1937 Size: 517 369 140 4594 Fragments arranged by size: 4594 517 369 140 Acc65I G'GTAC_C Cuts at: 2941 2941 Size: 5620 AclI AA'CG_TT Cuts at: 1563 3747 4120 1563 Size: 2184 373 3063 Fragments arranged by size: 3063 2184 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2616 3683 5067 5335 1632 Size: 984 1067 1384 268 1917 Fragments arranged by size: 1917 1384 1067 984 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5423 AflIII A'CryG_T Cuts at: 1203 4519 5496 1203 Size: 3316 977 1327 Fragments arranged by size: 3316 1327 977 AgeI A'CCGG_T Cuts at: 4451 4451 Size: 5620 AhdI GACnn_n'nnGTC Cuts at: 4349 4349 Size: 5620 AleI CACnn'nnGTG Cuts at: 1166 2534 1166 Size: 1368 4252 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2362 1184 Size: 1178 4442 AlwNI CAG_nnn'CTG Cuts at: 2078 2137 3363 4935 2078 Size: 59 1226 1572 2763 Fragments arranged by size: 2763 1572 1226 59 ApaLI G'TGCA_C Cuts at: 3677 4833 3677 Size: 1156 4464 ApoI r'AATT_y Cuts at: 1493 2929 3155 3307 5416 1493 Size: 1436 226 152 2109 1697 Fragments arranged by size: 2109 1697 1436 226 152 AseI AT'TA_AT Cuts at: 160 1032 4174 160 Size: 872 3142 1606 Fragments arranged by size: 3142 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5620 AvaI C'yCGr_G Cuts at: 1870 1943 2945 1870 Size: 73 1002 4545 Fragments arranged by size: 4545 1002 73 BamHI G'GATC_C Cuts at: 2950 3317 2950 Size: 367 5253 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2941 4396 618 Size: 325 217 627 1154 1455 1842 Fragments arranged by size: 1842 1455 1154 627 325 217 BanII G_rGCy'C Cuts at: 1391 2217 2904 2939 1391 Size: 826 687 35 4072 Fragments arranged by size: 4072 826 687 35 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4649 BbvCI CC'TCA_GC Cuts at: 2068 2884 2068 Size: 816 4804 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2813 2847 3811 3845 1903 Size: 34 876 34 964 34 3678 Fragments arranged by size: 3678 964 876 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3512 4722 3512 Size: 1210 4410 BclI T'GATC_A Cuts at: 1548 1548 Size: 5620 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2432 4231 136 Size: 107 122 71 631 605 760 1799 1525 Fragments arranged by size: 1799 1525 760 631 605 122 107 71 BglII A'GATC_T Cuts at: 2341 2575 5615 2341 Size: 234 3040 2346 Fragments arranged by size: 3040 2346 234 BlpI GC'TnA_GC Cuts at: 3037 3037 Size: 5620 Bme1580I G_kGCm'C Cuts at: 1790 3681 4837 1790 Size: 1891 1156 2573 Fragments arranged by size: 2573 1891 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5620 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3351 4309 442 Size: 416 53 83 399 24 1934 958 1753 Fragments arranged by size: 1934 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5620 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2117 2380 4280 1239 Size: 878 263 1900 2579 Fragments arranged by size: 2579 1900 878 263 Bpu10I CC'TnA_GC Cuts at: 2068 2884 2068 Size: 816 4804 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2759 3745 4610 4908 2759 Size: 986 865 298 3471 Fragments arranged by size: 3471 986 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4283 882 Size: 617 2784 2219 Fragments arranged by size: 2784 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3411 493 Size: 711 2207 2702 Fragments arranged by size: 2702 2207 711 BsaBI GATnn'nnATC Cuts at: 5614 5614 Size: 5620 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2739 3427 3809 4444 Size: 53 83 186 936 1206 688 382 635 Cuts at: 4444 275 Size: 1451 Fragments arranged by size: 1451 1206 936 688 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2085 4053 4451 4725 4872 5283 2085 Size: 1968 398 274 147 411 2422 Fragments arranged by size: 2422 1968 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2378 2408 2801 2831 1178 Size: 30 165 30 975 30 393 30 3967 Fragments arranged by size: 3967 975 393 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2176 2822 1186 Size: 990 646 3984 Fragments arranged by size: 3984 990 646 BseYI C'CCAG_C Cuts at: 4823 5319 4823 Size: 496 5124 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5047 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3070 3831 4859 1540 Size: 275 186 1069 761 1028 2301 Fragments arranged by size: 2301 1069 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2217 2904 3681 3766 4837 2217 Size: 687 777 85 1071 3000 Fragments arranged by size: 3000 1071 777 687 85 BsmI GAATG_Cn' Cuts at: 3131 3224 3131 Size: 93 5527 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2217 2904 2939 3681 3766 4837 1391 Size: 399 427 687 35 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 687 427 399 85 35 BspEI T'CCGG_A Cuts at: 2085 5283 2085 Size: 3198 2422 BspHI T'CATG_A Cuts at: 2280 3509 5239 2280 Size: 1229 1730 2661 Fragments arranged by size: 2661 1730 1229 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2975 844 Size: 2131 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2290 3507 1394 Size: 429 467 1217 3507 Fragments arranged by size: 3507 1217 467 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4115 4289 65 Size: 1183 2867 174 1396 Fragments arranged by size: 2867 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4264 4451 1700 Size: 110 2454 187 2869 Fragments arranged by size: 2869 2454 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5620 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5620 BssSI C'ACGA_G Cuts at: 3680 4692 3680 Size: 1012 4608 BstAPI GCAn_nnn'nTGC Cuts at: 794 2078 794 Size: 1284 4336 BstBI TT'CG_AA Cuts at: 4424 4424 Size: 5620 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5620 BstXI CCAn_nnnn'nTGG Cuts at: 2647 2647 Size: 5620 BstYI r'GATC_y Cuts at: 1972 2053 2341 2575 2950 3317 3702 3719 5171 Size: 81 288 234 375 367 385 17 1452 Cuts at: 5171 5257 5269 5615 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 375 367 346 288 234 86 81 17 12 BstZ17I GTA'TAC Cuts at: 2455 2455 Size: 5620 BtgI C'CryG_G Cuts at: 513 1065 2004 2427 2816 513 Size: 552 939 423 389 3317 Fragments arranged by size: 3317 939 552 423 389 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2910 487 Size: 683 56 1684 3197 Fragments arranged by size: 3197 1684 683 56 BtsI GCAGTG_nn' Cuts at: 2079 2202 2829 3136 3930 3950 2079 Size: 123 627 307 794 20 3749 Fragments arranged by size: 3749 794 627 307 123 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5620 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5585 DraI TTT'AAA Cuts at: 2924 2924 Size: 5620 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4627 809 Size: 507 3311 1802 Fragments arranged by size: 3311 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2433 2496 3067 Size: 54 1425 50 275 18 603 63 571 Cuts at: 3067 3956 5585 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 603 571 275 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3067 1537 Size: 275 1255 4090 Fragments arranged by size: 4090 1255 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2102 2222 3550 1353 Size: 749 120 1328 3423 Fragments arranged by size: 3423 1328 749 120 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4207 4581 4727 387 Size: 1064 138 2618 374 146 1280 Fragments arranged by size: 2618 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2215 2902 2215 Size: 687 4933 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2117 2380 2616 3683 4280 5067 5335 Size: 393 485 263 236 1067 597 787 268 Cuts at: 5335 1239 Size: 1524 Fragments arranged by size: 1524 1067 787 597 485 393 268 263 236 EcoNI CCTnn'n_nnAGG Cuts at: 2804 2804 Size: 5620 EcoRI G'AATT_C Cuts at: 2929 3307 2929 Size: 378 5242 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2594 2594 Size: 5620 FspI TGC'GCA Cuts at: 4126 4126 Size: 5620 HaeII r_GCGC'y Cuts at: 1678 4767 1678 Size: 3089 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2051 2083 2131 2163 2784 Size: 32 135 32 479 32 48 32 621 Cuts at: 2784 2816 4261 4293 4335 4367 1373 Size: 32 1445 32 42 32 2626 Fragments arranged by size: 2626 1445 621 479 135 48 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2824 2964 3210 669 Size: 988 281 886 140 246 3079 Fragments arranged by size: 3079 988 886 281 246 140 HindIII A'AGCT_T Cuts at: 748 748 Size: 5620 HpaI GTT'AAC Cuts at: 3210 3210 Size: 5620 KpnI G_GTAC'C Cuts at: 2945 2945 Size: 5620 MfeI C'AATT_G Cuts at: 3219 3219 Size: 5620 MluI A'CGCG_T Cuts at: 5496 5496 Size: 5620 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4734 4918 1305 Size: 3429 184 2007 Fragments arranged by size: 3429 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2498 10 Size: 54 1425 343 666 3132 Fragments arranged by size: 3132 1425 666 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2534 3578 3937 4096 518 Size: 648 1368 1044 359 159 2042 Fragments arranged by size: 2042 1368 1044 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3036 3713 4861 5106 1606 Size: 1430 677 1148 245 2120 Fragments arranged by size: 2120 1430 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4129 Fragments arranged by size: 4129 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5620 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5620 NotI GC'GGCC_GC Cuts at: 3067 3067 Size: 5620 NspI r_CATG'y Cuts at: 1132 1229 2978 4523 1132 Size: 97 1749 1545 2229 Fragments arranged by size: 2229 1749 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5620 PciI A'CATG_T Cuts at: 4519 4519 Size: 5620 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5620 PfoI T'CCnGG_A Cuts at: 2415 2415 Size: 5620 PmeI GTTT'AAAC Cuts at: 2924 2924 Size: 5620 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2289 2321 3689 3721 5202 5234 2289 Size: 32 1368 32 1481 32 2675 Fragments arranged by size: 2675 1481 1368 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5620 PsiI TTA'TAA Cuts at: 3190 3190 Size: 5620 PstI C_TGCA'G Cuts at: 830 2972 830 Size: 2142 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5620 SacI G_AGCT'C Cuts at: 2217 2904 2217 Size: 687 4933 SalI G'TCGA_C Cuts at: 1936 2822 2962 1936 Size: 886 140 4594 Fragments arranged by size: 4594 886 140 SapI GCTCTTCn'nnn_ Cuts at: 2222 2222 Size: 5620 SbfI CC_TGCA'GG Cuts at: 2972 2972 Size: 5620 ScaI AGT'ACT Cuts at: 3868 5580 3868 Size: 1712 3908 SfcI C'TryA_G Cuts at: 826 1045 2968 4103 4784 4975 826 Size: 219 1923 1135 681 191 1471 Fragments arranged by size: 1923 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5620 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5620 SmaI CCC'GGG Cuts at: 1872 2947 1872 Size: 1075 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2774 3724 4625 4887 820 Size: 197 926 831 950 901 262 1553 Fragments arranged by size: 1553 950 926 901 831 262 197 SnaBI TAC'GTA Cuts at: 493 3411 493 Size: 2918 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5620 SphI G_CATG'C Cuts at: 1132 2978 1132 Size: 1846 3774 SspI AAT'ATT Cuts at: 5 52 2379 3544 5 Size: 47 2327 1165 2081 Fragments arranged by size: 2327 2081 1165 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5620 StyI C'CwwG_G Cuts at: 513 1065 2004 3059 513 Size: 552 939 1055 3074 Fragments arranged by size: 3074 1055 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3647 3664 3817 4002 4447 325 Size: 1255 2067 17 153 185 445 1498 Fragments arranged by size: 2067 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3866 5578 96 Size: 274 80 33 51 1443 1889 1712 138 Fragments arranged by size: 1889 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2378 2410 2801 2833 5580 5612 2378 Size: 32 391 32 2747 32 2386 Fragments arranged by size: 2747 2386 391 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5620 XbaI T'CTAG_A Cuts at: 2956 2956 Size: 5620 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5620 XmaI C'CCGG_G Cuts at: 1870 2945 1870 Size: 1075 4545 XmnI GAAnn'nnTTC Cuts at: 2301 3749 2301 Size: 1448 4172 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2740 3428 276 Size: 53 83 186 936 1206 688 2468 Fragments arranged by size: 2468 1206 936 688 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI Bsu36I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI