Restriction map for pFN21AB6405
 (Linear) (Six-base) MAPSORT of: KIBB6405.seq  Check: 63  from: 1  to: 915



KIBB6405.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AatII G_ACGT'C

Cuts at:      0     737     915 
   Size:        737     178
 
AccI GT'mk_AC

Cuts at:      0     449     818     915 
   Size:        449     369      97

  Fragments arranged by size: 

                 449     369      97
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     611     915 
   Size:        611     304
 
AleI CACnn'nnGTG

Cuts at:      0     529     915 
   Size:        529     386
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     357     915 
   Size:        357     558
 
AlwNI CAG_nnn'CTG

Cuts at:      0      73     132     915 
   Size:         73      59     783

  Fragments arranged by size: 

                 783      73      59
 
BanII G_rGCy'C

Cuts at:      0     212     899     915 
   Size:        212     687      16

  Fragments arranged by size: 

                 687     212      16
 
BbvCI CC'TCA_GC

Cuts at:      0      63     879     915 
   Size:         63     816      36

  Fragments arranged by size: 

                 816      63      36
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     808     842     915 
   Size:        808      34      73

  Fragments arranged by size: 

                 808      73      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     427     915 
   Size:        427     488
 
BglII A'GATC_T

Cuts at:      0     336     570     915 
   Size:        336     234     345

  Fragments arranged by size: 

                 345     336     234
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     375     915 
   Size:        112     263     540

  Fragments arranged by size: 

                 540     263     112
 
Bpu10I CC'TnA_GC

Cuts at:      0      63     879     915 
   Size:         63     816      36

  Fragments arranged by size: 

                 816      63      36
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     754     915 
   Size:        754     161
 
BsaHI Gr'CG_yC

Cuts at:      0     734     915 
   Size:        734     181
 
BsaWI w'CCGG_w

Cuts at:      0      80     915 
   Size:         80     835
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     373     403     796     826     915 
   Size:        373      30     393      30      89

  Fragments arranged by size: 

                 393     373      89      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     171     817     915 
   Size:        171     646      98

  Fragments arranged by size: 

                 646     171      98
 
BsiHKAI G_wGCw'C

Cuts at:      0     212     899     915 
   Size:        212     687      16

  Fragments arranged by size: 

                 687     212      16
 
Bsp1286I G_dGCh'C

Cuts at:      0     212     899     915 
   Size:        212     687      16

  Fragments arranged by size: 

                 687     212      16
 
BspEI T'CCGG_A

Cuts at:      0      80     915 
   Size:         80     835
 
BspHI T'CATG_A

Cuts at:      0     275     915 
   Size:        275     640
 
BsrBI CCG'CTC

Cuts at:      0     285     915 
   Size:        285     630
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      73     915 
   Size:         73     842
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     642     915 
   Size:        642     273
 
BstYI r'GATC_y

Cuts at:      0      48     336     570     915 
   Size:         48     288     234     345

  Fragments arranged by size: 

                 345     288     234      48
 
BstZ17I GTA'TAC

Cuts at:      0     450     915 
   Size:        450     465
 
BtgI C'CryG_G

Cuts at:      0     422     811     915 
   Size:        422     389     104

  Fragments arranged by size: 

                 422     389     104
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     905     915 
   Size:        905      10
 
BtsI GCAGTG_nn'

Cuts at:      0      74     197     824     915 
   Size:         74     123     627      91

  Fragments arranged by size: 

                 627     123      91      74
 
EaeI y'GGCC_r

Cuts at:      0     428     491     915 
   Size:        428      63     424

  Fragments arranged by size: 

                 428     424      63
 
EarI CTCTTCn'nnn_

Cuts at:      0      97     217     915 
   Size:         97     120     698

  Fragments arranged by size: 

                 698     120      97
 
EcoICRI GAG'CTC

Cuts at:      0     210     897     915 
   Size:        210     687      18

  Fragments arranged by size: 

                 687     210      18
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     375     611     915 
   Size:        112     263     236     304

  Fragments arranged by size: 

                 304     263     236     112
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     799     915 
   Size:        799     116
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     589     915 
   Size:        589     326
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      46      78     126     158     779     811     915 
   Size:         46      32      48      32     621      32     104

  Fragments arranged by size: 

                 621     104      48      46      32      32      32
 
HincII GTy'rAC

Cuts at:      0     819     915 
   Size:        819      96
 
MscI TGG'CCA

Cuts at:      0     493     915 
   Size:        493     422
 
MslI CAynn'nnrTG

Cuts at:      0     529     915 
   Size:        529     386
 
PfoI T'CCnGG_A

Cuts at:      0     410     915 
   Size:        410     505
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     284     316     915 
   Size:        284      32     599

  Fragments arranged by size: 

                 599     284      32
 
SacI G_AGCT'C

Cuts at:      0     212     899     915 
   Size:        212     687      16

  Fragments arranged by size: 

                 687     212      16
 
SalI G'TCGA_C

Cuts at:      0     817     915 
   Size:        817      98
 
SapI GCTCTTCn'nnn_

Cuts at:      0     217     915 
   Size:        217     698
 
SmlI C'TyrA_G

Cuts at:      0     769     915 
   Size:        769     146
 
SspI AAT'ATT

Cuts at:      0     374     915 
   Size:        374     541
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     373     405     796     828     915 
   Size:        373      32     391      32      87

  Fragments arranged by size: 

                 391     373      87      32      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     296     915 
   Size:        296     619
 
ZraI GAC'GTC

Cuts at:      0     735     915 
   Size:        735     180
 

 Enzymes that do cut:

    AatII     AccI     AcuI     AleI     AlfI    AlwNI    BanII    BbvCI
     BcgI     BglI    BglII     BpmI   Bpu10I    BpuEI    BsaHI    BsaWI
    BsaXI    BseRI  BsiHKAI Bsp1286I    BspEI    BspHI    BsrBI   BstAPI
    BstXI    BstYI  BstZ17I     BtgI    BtgZI     BtsI     EaeI     EarI
  EcoICRI  Eco57MI    EcoNI     FalI    Hin4I   HincII     MscI     MslI
     PfoI     PpiI     SacI     SalI     SapI     SmlI     SspI     TstI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BciVI     BclI
    BfrBI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BsaI
    BsaAI    BsaBI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII    SacII    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     StyI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI
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