Restriction map for pFN21AB6460
 (Circular) (Six-base) MAPSORT of: pFN21AB6460.seq  Check: 7896  from: 1  to: 5758



pFN21AB6460  5758 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:39  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3568     278 
   Size:         53      83     186     936    2032    2468

  Fragments arranged by size: 

                2468    2032     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3101    1937 
   Size:       1164    4594
 
Acc65I G'GTAC_C

Cuts at:   3079    3079 
   Size:       5758
 
AclI AA'CG_TT

Cuts at:   1563    3885    4258    1563 
   Size:       2322     373    3063

  Fragments arranged by size: 

                3063    2322     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2528    2773    3821    5205    5473    1632 
   Size:        896     245    1048    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1048     896     268     245
 
AfeI AGC'GCT

Cuts at:   2846    2846 
   Size:       5758
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5561
 
AflIII A'CryG_T

Cuts at:   1203    4657    5634    1203 
   Size:       3454     977    1327

  Fragments arranged by size: 

                3454    1327     977
 
AgeI A'CCGG_T

Cuts at:   4589    4589 
   Size:       5758
 
AhdI GACnn_n'nnGTC

Cuts at:   4487    4487 
   Size:       5758
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5758
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5758
 
AlwNI CAG_nnn'CTG

Cuts at:   3501    5073    3501 
   Size:       1572    4186
 
ApaI G_GGCC'C

Cuts at:   2677    2677 
   Size:       5758
 
ApaLI G'TGCA_C

Cuts at:   3815    4971    3815 
   Size:       1156    4602
 
ApoI r'AATT_y

Cuts at:   1493    2057    3067    3293    3445    5554    1493 
   Size:        564    1010     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1010     564     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4312     160 
   Size:        872    3280    1606

  Fragments arranged by size: 

                3280    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5758
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2324    3083    1870 
   Size:         73     381     759    4545

  Fragments arranged by size: 

                4545     759     381      73
 
BamHI G'GATC_C

Cuts at:   3088    3455    3088 
   Size:        367    5391
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2606    3079    4534     618 
   Size:        325     217     627     819     473    1455    1842

  Fragments arranged by size: 

                1842    1455     819     627     473     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2165    2430    2677    3077    1391 
   Size:        774     265     247     400    4072

  Fragments arranged by size: 

                4072     774     400     265     247
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4787
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3949    3983    1903 
   Size:         34    2012      34    3678

  Fragments arranged by size: 

                3678    2012      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2968    3650    4860    2968 
   Size:        682    1210    3866

  Fragments arranged by size: 

                3866    1210     682
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5758
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4369     136 
   Size:        107     122      71     631     605    2697    1525

  Fragments arranged by size: 

                2697    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2742    2833    5753    2742 
   Size:         91    2920    2747

  Fragments arranged by size: 

                2920    2747      91
 
BlpI GC'TnA_GC

Cuts at:   3175    3175 
   Size:       5758
 
Bme1580I G_kGCm'C

Cuts at:   1790    2379    2677    3819    4975    1790 
   Size:        589     298    1142    1156    2573

  Fragments arranged by size: 

                2573    1156    1142     589     298
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5758
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3489    4447     442 
   Size:        416      53      83     399      24    2072     958    1753

  Fragments arranged by size: 

                2072    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5758
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2410    2410 
   Size:       5758
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4418    1239 
   Size:       3179    2579
 
Bpu10I CC'TnA_GC

Cuts at:   2639    2639 
   Size:       5758
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2843    3883    4748    5046    2843 
   Size:       1040     865     298    3555

  Fragments arranged by size: 

                3555    1040     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2192    3022    4421     882 
   Size:        617     693     830    1399    2219

  Fragments arranged by size: 

                2219    1399     830     693     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2374    3549     493 
   Size:        711    1170    1175    2702

  Fragments arranged by size: 

                2702    1175    1170     711
 
BsaBI GATnn'nnATC

Cuts at:   2918    5752    2918 
   Size:       2834    2924
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3565    3947    4582     275 
   Size:         53      83     186     936    2032     382     635    1451

  Fragments arranged by size: 

                2032    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4191    4589    4863    5010    5421    4191 
   Size:        398     274     147     411    4528

  Fragments arranged by size: 

                4528     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5533

  Fragments arranged by size: 

                5533     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5758
 
BseYI C'CCAG_C

Cuts at:   4961    5457    4961 
   Size:        496    5262
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2462    1163 
   Size:        573     726    4459

  Fragments arranged by size: 

                4459     726     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3208    3969    4997    1540 
   Size:        275     186    1207     761    1028    2301

  Fragments arranged by size: 

                2301    1207    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3819    3904    4975    3819 
   Size:         85    1071    4602

  Fragments arranged by size: 

                4602    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3269    3362    3269 
   Size:         93    5665
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2764    2764 
   Size:       5758
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2165    2379    2430    2677    3077    3819    3904 
   Size:        399     375     214      51     247     400     742      85

Cuts at:   3904    4975    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     742     400     399     375     247     214
                  85      51
 
BspEI T'CCGG_A

Cuts at:   5421    5421 
   Size:       5758
 
BspHI T'CATG_A

Cuts at:   3647    5377    3647 
   Size:       1730    4028
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2817    2818    3113     844 
   Size:       1973       1     295    3489

  Fragments arranged by size: 

                3489    1973     295       1
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2108    3645    1394 
   Size:        429     285    1537    3507

  Fragments arranged by size: 

                3507    1537     429     285
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4253    4427      65 
   Size:       1183    3005     174    1396

  Fragments arranged by size: 

                3005    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4402    4589    1700 
   Size:        110    2592     187    2869

  Fragments arranged by size: 

                2869    2592     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5758
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5758
 
BssSI C'ACGA_G

Cuts at:   3818    4830    3818 
   Size:       1012    4746
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5758
 
BstBI TT'CG_AA

Cuts at:   4562    4562 
   Size:       5758
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5758
 
BstYI r'GATC_y

Cuts at:   1972    2223    2488    2742    2833    3088    3455    3840    3857 
   Size:        251     265     254      91     255     367     385      17

Cuts at:   3857    5309    5395    5407    5753    1972 
   Size:       1452      86      12     346    1977

  Fragments arranged by size: 

                1977    1452     385     367     346     265     255     254
                 251      91      86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2661     513 
   Size:        552     939     657    3610

  Fragments arranged by size: 

                3610     939     657     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5019

  Fragments arranged by size: 

                5019     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3274    4068    4088    3274 
   Size:        794      20    4944

  Fragments arranged by size: 

                4944     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5758
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5723
 
DraI TTT'AAA

Cuts at:   2962    3062    2962 
   Size:        100    5658
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4765     809 
   Size:        507    3449    1802

  Fragments arranged by size: 

                3449    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2700    3205    4094 
   Size:         54    1425      50     275      18     870     505     889

Cuts at:   4094    5723       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     870     505     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3205    1537 
   Size:        275    1393    4090

  Fragments arranged by size: 

                4090    1393     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3688    1353 
   Size:       2335    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2422    4345    4719    4865     387 
   Size:       1064     138     833    1923     374     146    1280

  Fragments arranged by size: 

                1923    1280    1064     833     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2528    2773    3821    4418    5205    5473    1239 
   Size:        393     896     245    1048     597     787     268    1524

  Fragments arranged by size: 

                1524    1048     896     787     597     393     268     245
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2825    2825 
   Size:       5758
 
EcoO109I rG'GnC_Cy

Cuts at:   2503    2674    2806    2966    2503 
   Size:        171     132     160    5295

  Fragments arranged by size: 

                5295     171     160     132
 
EcoRI G'AATT_C

Cuts at:   2057    3067    3445    2057 
   Size:       1010     378    4370

  Fragments arranged by size: 

                4370    1010     378
 
EcoRV GAT'ATC

Cuts at:   2878    2878 
   Size:       5758
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2102    2791    2102 
   Size:        689    5069
 
FspI TGC'GCA

Cuts at:   4264    4264 
   Size:       5758
 
HaeII r_GCGC'y

Cuts at:   1678    2848    4905    1678 
   Size:       1170    2057    2531

  Fragments arranged by size: 

                2531    2057    1170
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4399    4431    4473    4505    1373 
   Size:         32     135      32    2827      32      42      32    2626

  Fragments arranged by size: 

                2827    2626     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2302    2872    3102    3348     669 
   Size:        988     281     364     570     230     246    3079

  Fragments arranged by size: 

                3079     988     570     364     281     246     230
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5758
 
HpaI GTT'AAC

Cuts at:   3348    3348 
   Size:       5758
 
KpnI G_GTAC'C

Cuts at:   3083    3083 
   Size:       5758
 
MfeI C'AATT_G

Cuts at:   3357    3357 
   Size:       5758
 
MluI A'CGCG_T

Cuts at:   5634    5634 
   Size:       5758
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4872    5056    1305 
   Size:       3567     184    2007

  Fragments arranged by size: 

                3567    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3936

  Fragments arranged by size: 

                3936    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2363    2549    3716    4075    4234     518 
   Size:        648    1197     186    1167     359     159    2042

  Fragments arranged by size: 

                2042    1197    1167     648     359     186     159
 
MspA1I CmG'CkG

Cuts at:   1606    2705    3174    3851    4999    5244    1606 
   Size:       1099     469     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1099     677     469     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4267

  Fragments arranged by size: 

                4267     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5758
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5758
 
NotI GC'GGCC_GC

Cuts at:   3205    3205 
   Size:       5758
 
NspI r_CATG'y

Cuts at:   1132    1229    3116    4661    1132 
   Size:         97    1887    1545    2229

  Fragments arranged by size: 

                2229    1887    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    2677    2969    1348 
   Size:       1329     292    4137

  Fragments arranged by size: 

                4137    1329     292
 
PciI A'CATG_T

Cuts at:   4657    4657 
   Size:       5758
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5758
 
PmeI GTTT'AAAC

Cuts at:   3062    3062 
   Size:       5758
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3827    3859    5340    5372    3827 
   Size:         32    1481      32    4213

  Fragments arranged by size: 

                4213    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2503    2806    2503 
   Size:        303    5455
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5758
 
PsiI TTA'TAA

Cuts at:   3328    3328 
   Size:       5758
 
PspOMI G'GGCC_C

Cuts at:   2673    2673 
   Size:       5758
 
PspXI vC'TCGA_Gb

Cuts at:   2324    2324 
   Size:       5758
 
PstI C_TGCA'G

Cuts at:    830    2829    3110     830 
   Size:       1999     281    3478

  Fragments arranged by size: 

                3478    1999     281
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5758
 
SalI G'TCGA_C

Cuts at:   1936    3100    1936 
   Size:       1164    4594
 
SbfI CC_TGCA'GG

Cuts at:   2829    3110    2829 
   Size:        281    5477
 
ScaI AGT'ACT

Cuts at:   4006    5718    4006 
   Size:       1712    4046
 
SfcI C'TryA_G

Cuts at:    826    1045    2497    2825    3106    4241    4922    5113     826 
   Size:        219    1452     328     281    1135     681     191    1471

  Fragments arranged by size: 

                1471    1452    1135     681     328     281     219     191
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5758
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5758
 
SmaI CCC'GGG

Cuts at:   1872    3085    1872 
   Size:       1213    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2324    2858    3862    4763    5025     820 
   Size:        197     926     381     534    1004     901     262    1553

  Fragments arranged by size: 

                1553    1004     926     901     534     381     262     197
            
 
SnaBI TAC'GTA

Cuts at:    493    3549     493 
   Size:       3056    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5758
 
SphI G_CATG'C

Cuts at:   1132    3116    1132 
   Size:       1984    3774
 
SspI AAT'ATT

Cuts at:      5      52    3682       5 
   Size:         47    3630    2081

  Fragments arranged by size: 

                3630    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5758
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    3197     513 
   Size:        552     939    1193    3074

  Fragments arranged by size: 

                3074    1193     939     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3785    3802    3955    4140    4585     325 
   Size:       1255    2205      17     153     185     445    1498

  Fragments arranged by size: 

                2205    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4004    5716      96 
   Size:        274      80      33      51    1443    2027    1712     138

  Fragments arranged by size: 

                2027    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5718    5750    5718 
   Size:         32    5726
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5758
 
XbaI T'CTAG_A

Cuts at:   3094    3094 
   Size:       5758
 
XhoI C'TCGA_G

Cuts at:   1943    2324    1943 
   Size:        381    5377
 
XmaI C'CCGG_G

Cuts at:   1870    3083    1870 
   Size:       1213    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2057    2082    3887    2057 
   Size:         25    1805    3928

  Fragments arranged by size: 

                3928    1805      25
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3566     276 
   Size:         53      83     186     936    2032    2468

  Fragments arranged by size: 

                2468    2032     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PstI     PvuI     SalI
     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AloI     AscI    AvrII     BaeI     BbeI    BbvCI    BfrBI
    BsiWI    BstXI  BstZ17I   Bsu36I   DraIII  EcoICRI     FseI    FspAI
     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PfoI
     PmlI     PsrI    PvuII    RsrII     SacI    SacII    SanDI     SapI
    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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