Restriction map for
pFN21AB6368
(Linear) (Six-base) MAPSORT of: KIBB6368.seq Check: 9844 from: 1 to: 894
KIBB6368.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 189 894
Size: 189 705
AccI GT'mk_AC
Cuts at: 0 479 894
Size: 479 415
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 894
Size: 239 655
AleI CACnn'nnGTG
Cuts at: 0 388 894
Size: 388 506
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 76 550 894
Size: 76 474 344
Fragments arranged by size:
474 344 76
ApoI r'AATT_y
Cuts at: 0 658 894
Size: 658 236
BanII G_rGCy'C
Cuts at: 0 524 704 894
Size: 524 180 190
Fragments arranged by size:
524 190 180
BbsI GAAGACnn'nnnn_
Cuts at: 0 191 283 383 894
Size: 191 92 100 511
Fragments arranged by size:
511 191 100 92
Bme1580I G_kGCm'C
Cuts at: 0 566 852 894
Size: 566 286 42
Fragments arranged by size:
566 286 42
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 531 894
Size: 531 363
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 453 894
Size: 287 166 441
Fragments arranged by size:
441 287 166
Bpu10I CC'TnA_GC
Cuts at: 0 97 894
Size: 97 797
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 838 894
Size: 838 56
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 329 359 894
Size: 329 30 535
Fragments arranged by size:
535 329 30
BseYI C'CCAG_C
Cuts at: 0 122 135 333 765 794 894
Size: 122 13 198 432 29 100
Fragments arranged by size:
432 198 122 100 29 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 894
Size: 22 872
BsiEI CG_ry'CG
Cuts at: 0 781 894
Size: 781 113
BsiHKAI G_wGCw'C
Cuts at: 0 103 524 704 893 894
Size: 103 421 180 189 1
Fragments arranged by size:
421 189 180 103 1
Bsp1286I G_dGCh'C
Cuts at: 0 103 524 566 704 852 893 894
Size: 103 421 42 138 148 41 1
Fragments arranged by size:
421 148 138 103 42 41 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 189 894
Size: 189 705
BsrBI CCG'CTC
Cuts at: 0 863 894
Size: 863 31
BsrFI r'CCGG_y
Cuts at: 0 41 780 894
Size: 41 739 114
Fragments arranged by size:
739 114 41
BssHII G'CGCG_C
Cuts at: 0 798 894
Size: 798 96
BssSI C'ACGA_G
Cuts at: 0 574 894
Size: 574 320
BstXI CCAn_nnnn'nTGG
Cuts at: 0 88 194 390 894
Size: 88 106 196 504
Fragments arranged by size:
504 196 106 88
BstYI r'GATC_y
Cuts at: 0 261 894
Size: 261 633
BstZ17I GTA'TAC
Cuts at: 0 480 894
Size: 480 414
BtgI C'CryG_G
Cuts at: 0 73 894
Size: 73 821
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 471 894
Size: 471 423
BtsI GCAGTG_nn'
Cuts at: 0 58 468 894
Size: 58 410 426
Fragments arranged by size:
426 410 58
DraIII CAC_nnn'GTG
Cuts at: 0 693 894
Size: 693 201
EaeI y'GGCC_r
Cuts at: 0 90 287 695 752 778 782 884 894
Size: 90 197 408 57 26 4 102 10
Fragments arranged by size:
408 197 102 90 57 26 10 4
EagI C'GGCC_G
Cuts at: 0 778 894
Size: 778 116
EarI CTCTTCn'nnn_
Cuts at: 0 617 709 894
Size: 617 92 185
Fragments arranged by size:
617 185 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 663 894
Size: 663 231
EcoICRI GAG'CTC
Cuts at: 0 522 702 894
Size: 522 180 192
Fragments arranged by size:
522 192 180
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 287 453 894
Size: 239 48 166 441
Fragments arranged by size:
441 239 166 48
EcoO109I rG'GnC_Cy
Cuts at: 0 318 356 894
Size: 318 38 538
Fragments arranged by size:
538 318 38
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 300 609 894
Size: 300 309 285
Fragments arranged by size:
309 300 285
FseI GG_CCGG'CC
Cuts at: 0 784 894
Size: 784 110
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 181 213 894
Size: 181 32 681
Fragments arranged by size:
681 181 32
MscI TGG'CCA
Cuts at: 0 289 894
Size: 289 605
MslI CAynn'nnrTG
Cuts at: 0 388 574 894
Size: 388 186 320
Fragments arranged by size:
388 320 186
MspA1I CmG'CkG
Cuts at: 0 333 534 602 894
Size: 333 201 68 292
Fragments arranged by size:
333 292 201 68
NaeI GCC'GGC
Cuts at: 0 782 894
Size: 782 112
NgoMIV G'CCGG_C
Cuts at: 0 780 894
Size: 780 114
PflMI CCAn_nnn'nTGG
Cuts at: 0 882 894
Size: 882 12
PfoI T'CCnGG_A
Cuts at: 0 264 894
Size: 264 630
PpuMI rG'GwC_Cy
Cuts at: 0 318 356 894
Size: 318 38 538
Fragments arranged by size:
538 318 38
PstI C_TGCA'G
Cuts at: 0 455 686 894
Size: 455 231 208
Fragments arranged by size:
455 231 208
PvuII CAG'CTG
Cuts at: 0 333 534 894
Size: 333 201 360
Fragments arranged by size:
360 333 201
SacI G_AGCT'C
Cuts at: 0 524 704 894
Size: 524 180 190
Fragments arranged by size:
524 190 180
SapI GCTCTTCn'nnn_
Cuts at: 0 709 894
Size: 709 185
SbfI CC_TGCA'GG
Cuts at: 0 455 894
Size: 455 439
SfcI C'TryA_G
Cuts at: 0 451 682 894
Size: 451 231 212
Fragments arranged by size:
451 231 212
SgrAI Cr'CCGG_yG
Cuts at: 0 41 894
Size: 41 853
SmlI C'TyrA_G
Cuts at: 0 853 894
Size: 853 41
StyI C'CwwG_G
Cuts at: 0 755 894
Size: 755 139
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 181 509 894
Size: 181 328 385
Fragments arranged by size:
385 328 181
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 573 894
Size: 573 321
Enzymes that do cut:
AarI AccI AcuI AleI AlfI ApoI BanII BbsI
Bme1580I BplI BpmI Bpu10I BpuEI BsaXI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI
BstXI BstYI BstZ17I BtgI BtgZI BtsI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I FalI FseI
Hin4I MscI MslI MspA1I NaeI NgoMIV PflMI PfoI
PpuMI PstI PvuII SacI SapI SbfI SfcI SgrAI
SmlI StyI TaqII XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrGI BstAPI BstBI BstEII
Bsu36I ClaI CspCI DraI DrdI EcoNI EcoRI EcoRV
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NarI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI ScaI SexAI SfiI SfoI SgfI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI