Restriction map for
pFN21AE0990
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AvaI AviII AvrII BamHI
BbeI BbrPI BcgI BciVI BfaI
BfrI BglI BlnI BsaBI BsaI
BsiEI BsiWI BsmI BsoBI Bsp1407I
BspDI BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HaeII HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MseI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SspBI SwaI Tru9I Tsp45I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 670 GTCTAC GT^[AC][GT]AC
AcsI 1292 AAATTC [GA]^AATT[CT]
AlwI 742 GGATC GGATC(4/5)
ApoI 1292 AAATTC [GA]^AATT[CT]
Asp700I 693 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 604 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 1635 GGCACC G^G[CT][GA]CC
BbvCI 367 GCTGAGG CCTCAGC(-5/-2)
BclI 104 TGATCA T^GATCA
BglII 1358 AGATCT A^GATCT
BlpI 66 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 66 GCTCAGC GC^T[ACGT]AGC
BsaAI 100 TACGTG [CT]AC^GT[GA]
BsgI 151 GTGCAG GTGCAG(16/14)
BsmBI 1519 GAGACG CGTCTC(1/5)
BsrDI 1240 GCAATG GCAATG(2/0)
BstAPI 1645 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1625 CCATGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1358 AGATCT [GA]^GATC[CT]
CelII 66 GCTCAGC GC^T[ACGT]AGC
DrdI 890 GACTGCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 104 TGATCA T^GATCA
HgaI 61 GACGC GACGC(5/10)
MflI 1358 AGATCT [GA]^GATC[CT]
NaeI 450 GCCGGC GCC^GGC
NcoI 140 CCATGG C^CATGG
NgoMIV 450 GCCGGC G^CCGGC
NspI 194 GCATGC [GA]CATG^[CT]
PflFI 604 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SbfI 1464 CCTGCAGG CCTGCA^GG
SmlI 492 CTTGAG C^T[CT][GA]AG
SphI 194 GCATGC GCATG^C
Sse8387I 1464 CCTGCAGG CCTGCA^GG
SspI 1822 AATATT AAT^ATT
StuI 1733 AGGCCT AGG^CCT
TspRI 1287 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 604 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1358 AGATCT [GA]^GATC[CT]
XmnI 693 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AatII 667 GACGTC GACGT^C
1128 GACGTC
AccIII 1124 TCCGGA T^CCGGA
1398 TCCGGA
AcyI 667 GACGTC G[GA]^CG[CT]C
1128 GACGTC
BalI 404 TGGCCA TGG^CCA
1421 TGGCCA
BsaHI 667 GACGTC G[GA]^CG[CT]C
1128 GACGTC
BsaWI 1124 TCCGGA [AT]^CCGG[AT]
1398 TCCGGA
BseAI 1124 TCCGGA T^CCGGA
1398 TCCGGA
BspEI 1124 TCCGGA T^CCGGA
1398 TCCGGA
BspMI 974 ACCTGC ACCTGC(4/8)
1118 ACCTGC
BsrBI 145 GAGCGG CCGCTC(-3/-3)
1501 GAGCGG
BsrFI 282 ACCGGC [GA]^CCGG[CT]
450 GCCGGC
BsrI 80 ACTGG ACTGG(1/-1)
275 ACTGG
Cfr10I 282 ACCGGC [GA]^CCGG[CT]
450 GCCGGC
DsaI 140 CCATGG C^C[GA][CT]GG
1424 CCACGG
EcoNI 1374 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1464 CCTGCAGGAGG
EcoT14I 140 CCATGG C^C[AT][AT]GG
1531 CCTTGG
Hin1I 667 GACGTC G[GA]^CG[CT]C
1128 GACGTC
HincII 1081 GTCAAC GT[CT]^[GA]AC
1594 GTTGAC
HindII 1081 GTCAAC GT[CT]^[GA]AC
1594 GTTGAC
MaeIII 1454 GTAAC ^GT[ACGT]AC
1765 GTTAC
MluNI 404 TGGCCA TGG^CCA
1421 TGGCCA
MroI 1124 TCCGGA T^CCGGA
1398 TCCGGA
MscI 404 TGGCCA TGG^CCA
1421 TGGCCA
PstI 892 CTGCAG CTGCA^G
1465 CTGCAG
SapI 1498 GAAGAGC GCTCTTC(1/4)
1559 GCTCTTC
StyI 140 CCATGG C^C[AT][AT]GG
1531 CCTTGG
TaqI 749 TCGA T^CGA
968 TCGA
TfiI 520 GATTC G^A[AT]TC
755 GATTC
TthHB8I 749 TCGA T^CGA
968 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaII BanII BbsI BbvI
BcnI BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI TseI Tsp509I