(Circular) (Six-base) MAPSORT of: pFN21AE0990.seq Check: 1400 from: 1 to: 6580 pFN21AE0990 6580 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3132 3132 Size: 6580 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2676 3137 4390 278 Size: 53 83 186 936 1140 461 1253 2468 Fragments arranged by size: 2468 1253 1140 936 461 186 83 53 AccI GT'mk_AC Cuts at: 1937 2676 3923 1937 Size: 739 1247 4594 Fragments arranged by size: 4594 1247 739 Acc65I G'GTAC_C Cuts at: 3901 3901 Size: 6580 AclI AA'CG_TT Cuts at: 1563 4707 5080 1563 Size: 3144 373 3063 Fragments arranged by size: 3144 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2496 2521 3184 3296 4643 6027 6295 1632 Size: 864 25 663 112 1347 1384 268 1917 Fragments arranged by size: 1917 1384 1347 864 663 268 112 25 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6383 AflIII A'CryG_T Cuts at: 1203 5479 6456 1203 Size: 4276 977 1327 Fragments arranged by size: 4276 1327 977 AgeI A'CCGG_T Cuts at: 5411 5411 Size: 6580 AhdI GACnn_n'nnGTC Cuts at: 5309 5309 Size: 6580 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6580 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6580 AlwNI CAG_nnn'CTG Cuts at: 2360 2949 3656 4323 5895 2360 Size: 589 707 667 1572 3045 Fragments arranged by size: 3045 1572 707 667 589 ApaLI G'TGCA_C Cuts at: 4637 5793 4637 Size: 1156 5424 ApoI r'AATT_y Cuts at: 1493 3297 3889 4115 4267 6376 1493 Size: 1804 592 226 152 2109 1697 Fragments arranged by size: 2109 1804 1697 592 226 152 AseI AT'TA_AT Cuts at: 160 1032 5134 160 Size: 872 4102 1606 Fragments arranged by size: 4102 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6580 AvaI C'yCGr_G Cuts at: 1870 1943 3905 1870 Size: 73 1962 4545 Fragments arranged by size: 4545 1962 73 BamHI G'GATC_C Cuts at: 3910 4277 3910 Size: 367 6213 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3640 3901 5356 618 Size: 325 217 627 1853 261 1455 1842 Fragments arranged by size: 1853 1842 1455 627 325 261 217 BanII G_rGCy'C Cuts at: 1391 2253 2449 3020 3870 3899 1391 Size: 862 196 571 850 29 4072 Fragments arranged by size: 4072 862 850 571 196 29 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2701 3122 3338 928 Size: 971 802 421 216 4170 Fragments arranged by size: 4170 971 802 421 216 BbvCI CC'TCA_GC Cuts at: 2373 2373 Size: 6580 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4771 4805 1903 Size: 34 2834 34 3678 Fragments arranged by size: 3678 2834 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4472 5682 4472 Size: 1210 5370 BclI T'GATC_A Cuts at: 1548 2109 1548 Size: 561 6019 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5191 136 Size: 107 122 71 631 605 3519 1525 Fragments arranged by size: 3519 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3363 6575 3363 Size: 3212 3368 BlpI GC'TnA_GC Cuts at: 2072 3997 2072 Size: 1925 4655 Bme1580I G_kGCm'C Cuts at: 1790 2688 4641 5797 1790 Size: 898 1953 1156 2573 Fragments arranged by size: 2573 1953 1156 898 BmgBI CAC'GTC Cuts at: 1318 2611 1318 Size: 1293 5287 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4311 5269 442 Size: 416 53 83 399 24 2894 958 1753 Fragments arranged by size: 2894 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6580 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2116 3742 2116 Size: 1626 4954 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2267 2421 2450 2601 2617 3011 3310 3401 Size: 1028 154 29 151 16 394 299 91 Cuts at: 3401 3773 5240 1239 Size: 372 1467 2579 Fragments arranged by size: 2579 1467 1028 394 372 299 154 151 91 29 16 Bpu10I CC'TnA_GC Cuts at: 2373 2553 2953 2373 Size: 180 400 6000 Fragments arranged by size: 6000 400 180 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2518 4705 5570 5868 2518 Size: 2187 865 298 3230 Fragments arranged by size: 3230 2187 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5243 882 Size: 617 3744 2219 Fragments arranged by size: 3744 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2107 4371 493 Size: 711 903 2264 2702 Fragments arranged by size: 2702 2264 903 711 BsaBI GATnn'nnATC Cuts at: 6574 6574 Size: 6580 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2673 3134 4387 4769 Size: 53 83 186 936 1140 461 1253 382 Cuts at: 4769 5404 275 Size: 635 1451 Fragments arranged by size: 1451 1253 1140 936 635 461 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3129 3403 5013 5411 5685 5832 6243 3129 Size: 274 1610 398 274 147 411 3466 Fragments arranged by size: 3466 1610 411 398 274 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2171 2201 1178 Size: 30 165 30 768 30 5557 Fragments arranged by size: 5557 768 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3278 3613 3616 3718 3721 1186 Size: 2092 335 3 102 3 4045 Fragments arranged by size: 4045 2092 335 102 3 3 BseYI C'CCAG_C Cuts at: 2124 2821 2934 5783 6279 2124 Size: 697 113 2849 496 2425 Fragments arranged by size: 2849 2425 697 496 113 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2177 1163 Size: 573 441 5566 Fragments arranged by size: 5566 573 441 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4030 4791 5819 1540 Size: 275 186 2029 761 1028 2301 Fragments arranged by size: 2301 2029 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2253 3020 3870 4641 4726 5797 2253 Size: 767 850 771 85 1071 3036 Fragments arranged by size: 3036 1071 850 771 767 85 BsmI GAATG_Cn' Cuts at: 4091 4184 4091 Size: 93 6487 BsmBI CGTCTCn'nnnn_ Cuts at: 3518 3518 Size: 6580 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2253 2449 2688 3020 3870 3899 4641 Size: 399 463 196 239 332 850 29 742 Cuts at: 4641 4726 5797 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 850 742 463 399 332 239 196 85 29 BspEI T'CCGG_A Cuts at: 3129 3403 6243 3129 Size: 274 2840 3466 Fragments arranged by size: 3466 2840 274 BspHI T'CATG_A Cuts at: 4469 6199 4469 Size: 1730 4850 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2988 3132 3935 844 Size: 2144 144 803 3489 Fragments arranged by size: 3489 2144 803 144 BsrBI CCG'CTC Cuts at: 1394 1823 2152 3508 4467 1394 Size: 429 329 1356 959 3507 Fragments arranged by size: 3507 1356 959 429 329 BsrDI GCAATG_nn' Cuts at: 65 1248 3252 5075 5249 65 Size: 1183 2004 1823 174 1396 Fragments arranged by size: 2004 1823 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2287 2455 5224 5411 1700 Size: 110 477 168 2769 187 2869 Fragments arranged by size: 2869 2769 477 187 168 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6580 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6580 BssSI C'ACGA_G Cuts at: 4640 5652 4640 Size: 1012 5568 BstAPI GCAn_nnn'nTGC Cuts at: 794 3656 794 Size: 2862 3718 BstBI TT'CG_AA Cuts at: 5384 5384 Size: 6580 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6580 BstXI CCAn_nnnn'nTGG Cuts at: 3637 3637 Size: 6580 BstYI r'GATC_y Cuts at: 1972 3363 3910 4277 4662 4679 6131 6217 6229 Size: 1391 547 367 385 17 1452 86 12 Cuts at: 6229 6575 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1391 547 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2145 3429 513 Size: 552 939 141 1284 3664 Fragments arranged by size: 3664 1284 939 552 141 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5841 Fragments arranged by size: 5841 683 56 BtsI GCAGTG_nn' Cuts at: 4096 4890 4910 4096 Size: 794 20 5766 Fragments arranged by size: 5766 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6580 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3171 3206 529 Size: 35 2607 35 3903 Fragments arranged by size: 3903 2607 35 35 DraI TTT'AAA Cuts at: 3884 3884 Size: 6580 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2901 5587 809 Size: 507 1585 2686 1802 Fragments arranged by size: 2686 1802 1585 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2409 3219 3426 Size: 54 1425 50 275 18 579 810 207 Cuts at: 3426 4027 4916 6545 8 Size: 601 889 1629 43 Fragments arranged by size: 1629 1425 889 810 601 579 275 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4027 1537 Size: 275 2215 4090 Fragments arranged by size: 4090 2215 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2382 3498 3571 4510 1353 Size: 1029 1116 73 939 3423 Fragments arranged by size: 3423 1116 1029 939 73 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5167 5541 5687 387 Size: 1064 138 3578 374 146 1280 Fragments arranged by size: 3578 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2251 3018 3868 2251 Size: 767 850 4963 Fragments arranged by size: 4963 850 767 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2267 2421 2450 2496 2521 2601 2617 Size: 393 635 154 29 46 25 80 16 Cuts at: 2617 3011 3184 3296 3310 3401 3773 4643 5240 Size: 394 173 112 14 91 372 870 597 Cuts at: 5240 6027 6295 1239 Size: 787 268 1524 Fragments arranged by size: 1524 870 787 635 597 394 393 372 268 173 154 112 91 80 46 29 25 16 14 EcoNI CCTnn'n_nnAGG Cuts at: 3383 3473 3383 Size: 90 6490 EcoO109I rG'GnC_Cy Cuts at: 2430 2661 3226 3421 2430 Size: 231 565 195 5589 Fragments arranged by size: 5589 565 231 195 EcoRI G'AATT_C Cuts at: 3889 4267 3889 Size: 378 6202 FspI TGC'GCA Cuts at: 5086 5086 Size: 6580 HaeII r_GCGC'y Cuts at: 1678 5727 1678 Size: 4049 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3103 3135 3180 3212 3608 Size: 32 135 32 1531 32 45 32 396 Cuts at: 3608 3640 3716 3748 5221 5253 5295 5327 1373 Size: 32 76 32 1473 32 42 32 2626 Fragments arranged by size: 2626 1531 1473 396 135 76 45 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3088 3601 3924 4170 669 Size: 988 281 1150 513 323 246 3079 Fragments arranged by size: 3079 1150 988 513 323 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6580 HpaI GTT'AAC Cuts at: 4170 4170 Size: 6580 KpnI G_GTAC'C Cuts at: 3905 3905 Size: 6580 MfeI C'AATT_G Cuts at: 4179 4179 Size: 6580 MluI A'CGCG_T Cuts at: 6456 6456 Size: 6580 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3597 5694 5878 1305 Size: 2292 2097 184 2007 Fragments arranged by size: 2292 2097 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2411 3428 10 Size: 54 1425 343 579 1017 3162 Fragments arranged by size: 3162 1425 1017 579 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2805 3110 3167 3635 4538 4897 5056 Size: 648 1639 305 57 468 903 359 159 Cuts at: 5056 518 Size: 2042 Fragments arranged by size: 2042 1639 903 648 468 359 305 159 57 MspA1I CmG'CkG Cuts at: 1606 2026 2128 3178 3204 3996 4673 5821 6066 Size: 420 102 1050 26 792 677 1148 245 Cuts at: 6066 1606 Size: 2120 Fragments arranged by size: 2120 1148 1050 792 677 420 245 102 26 NaeI GCC'GGC Cuts at: 2457 2457 Size: 6580 NcoI C'CATG_G Cuts at: 513 1065 2004 2145 513 Size: 552 939 141 4948 Fragments arranged by size: 4948 939 552 141 NdeI CA'TA_TG Cuts at: 387 387 Size: 6580 NgoMIV G'CCGG_C Cuts at: 2455 2455 Size: 6580 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6580 NotI GC'GGCC_GC Cuts at: 4027 4027 Size: 6580 NspI r_CATG'y Cuts at: 1132 1229 2203 3938 5483 1132 Size: 97 974 1735 1545 2229 Fragments arranged by size: 2229 1735 1545 974 97 PasI CC'CwG_GG Cuts at: 1348 2426 3645 1348 Size: 1078 1219 4283 Fragments arranged by size: 4283 1219 1078 PciI A'CATG_T Cuts at: 5479 5479 Size: 6580 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6580 PfoI T'CCnGG_A Cuts at: 2743 3326 2743 Size: 583 5997 PmeI GTTT'AAAC Cuts at: 3884 3884 Size: 6580 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4649 4681 6162 6194 4649 Size: 32 1481 32 5035 Fragments arranged by size: 5035 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2430 2661 3226 2430 Size: 231 565 5784 Fragments arranged by size: 5784 565 231 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6580 PsiI TTA'TAA Cuts at: 4150 4150 Size: 6580 PstI C_TGCA'G Cuts at: 830 2901 3474 3932 830 Size: 2071 573 458 3478 Fragments arranged by size: 3478 2071 573 458 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6580 PvuII CAG'CTG Cuts at: 2026 2128 3178 3204 2026 Size: 102 1050 26 5402 Fragments arranged by size: 5402 1050 102 26 SacI G_AGCT'C Cuts at: 2253 3020 3870 2253 Size: 767 850 4963 Fragments arranged by size: 4963 850 767 SalI G'TCGA_C Cuts at: 1936 3922 1936 Size: 1986 4594 SapI GCTCTTCn'nnn_ Cuts at: 3498 3571 3498 Size: 73 6507 SbfI CC_TGCA'GG Cuts at: 3474 3932 3474 Size: 458 6122 ScaI AGT'ACT Cuts at: 4828 6540 4828 Size: 1712 4868 SfcI C'TryA_G Cuts at: 826 1045 2677 2885 2897 3470 3928 5063 5744 Size: 219 1632 208 12 573 458 1135 681 Cuts at: 5744 5935 826 Size: 191 1471 Fragments arranged by size: 1632 1471 1135 681 573 458 219 208 191 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6580 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6580 SmaI CCC'GGG Cuts at: 1872 3907 1872 Size: 2035 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2497 4684 5585 5847 820 Size: 197 926 554 2187 901 262 1553 Fragments arranged by size: 2187 1553 926 901 554 262 197 SnaBI TAC'GTA Cuts at: 493 4371 493 Size: 3878 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6580 SphI G_CATG'C Cuts at: 1132 2203 3938 1132 Size: 1071 1735 3774 Fragments arranged by size: 3774 1735 1071 SspI AAT'ATT Cuts at: 5 52 3829 4504 5 Size: 47 3777 675 2081 Fragments arranged by size: 3777 2081 675 47 StuI AGG'CCT Cuts at: 1517 3740 1517 Size: 2223 4357 StyI C'CwwG_G Cuts at: 513 1065 2004 2145 3536 4019 513 Size: 552 939 141 1391 483 3074 Fragments arranged by size: 3074 1391 939 552 483 141 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2026 3078 4607 4624 4777 4962 5407 Size: 1255 446 1052 1529 17 153 185 445 Cuts at: 5407 325 Size: 1498 Fragments arranged by size: 1529 1498 1255 1052 446 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4826 6538 96 Size: 274 80 33 51 1443 2849 1712 138 Fragments arranged by size: 2849 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2036 2068 2171 2203 2222 2254 2533 2565 6540 Size: 32 103 32 19 32 279 32 3975 Cuts at: 6540 6572 2036 Size: 32 2044 Fragments arranged by size: 3975 2044 279 103 32 32 32 32 32 19 Tth111I GACn'n_nGTC Cuts at: 1316 2612 1316 Size: 1296 5284 XbaI T'CTAG_A Cuts at: 3916 3916 Size: 6580 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6580 XmaI C'CCGG_G Cuts at: 1870 3905 1870 Size: 2035 4545 XmnI GAAnn'nnTTC Cuts at: 2702 4709 2702 Size: 2007 4573 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2674 3135 4388 276 Size: 53 83 186 936 1140 461 1253 2468 Fragments arranged by size: 2468 1253 1140 936 461 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI