Restriction map for
pFN21AE0990
(Linear) (Six-base) MAPSORT of: KIEE0990.seq Check: 2404 from: 1 to: 1875
KIEE0990.orf
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1127 1875
Size: 1127 748
AatII G_ACGT'C
Cuts at: 0 671 1132 1875
Size: 671 461 743
Fragments arranged by size:
743 671 461
AccI GT'mk_AC
Cuts at: 0 671 1875
Size: 671 1204
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 491 516 1179 1291 1875
Size: 491 25 663 112 584
Fragments arranged by size:
663 584 491 112 25
AlwNI CAG_nnn'CTG
Cuts at: 0 355 944 1651 1875
Size: 355 589 707 224
Fragments arranged by size:
707 589 355 224
ApoI r'AATT_y
Cuts at: 0 1292 1875
Size: 1292 583
BanI G'GyrC_C
Cuts at: 0 1635 1875
Size: 1635 240
BanII G_rGCy'C
Cuts at: 0 248 444 1015 1865 1875
Size: 248 196 571 850 10
Fragments arranged by size:
850 571 248 196 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 696 1117 1333 1875
Size: 696 421 216 542
Fragments arranged by size:
696 542 421 216
BbvCI CC'TCA_GC
Cuts at: 0 368 1875
Size: 368 1507
BclI T'GATC_A
Cuts at: 0 104 1875
Size: 104 1771
BglII A'GATC_T
Cuts at: 0 1358 1875
Size: 1358 517
BlpI GC'TnA_GC
Cuts at: 0 67 1875
Size: 67 1808
Bme1580I G_kGCm'C
Cuts at: 0 683 1875
Size: 683 1192
BmgBI CAC'GTC
Cuts at: 0 606 1875
Size: 606 1269
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 111 1737 1875
Size: 111 1626 138
Fragments arranged by size:
1626 138 111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 416 445 596 612 1006 1305 1396
Size: 262 154 29 151 16 394 299 91
Cuts at: 1396 1768 1875
Size: 372 107
Fragments arranged by size:
394 372 299 262 154 151 107 91
29 16
Bpu10I CC'TnA_GC
Cuts at: 0 368 548 948 1875
Size: 368 180 400 927
Fragments arranged by size:
927 400 368 180
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 513 1875
Size: 513 1362
BsaAI yAC'GTr
Cuts at: 0 102 1875
Size: 102 1773
BsaHI Gr'CG_yC
Cuts at: 0 668 1129 1875
Size: 668 461 746
Fragments arranged by size:
746 668 461
BsaWI w'CCGG_w
Cuts at: 0 1124 1398 1875
Size: 1124 274 477
Fragments arranged by size:
1124 477 274
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 166 196 1875
Size: 166 30 1679
Fragments arranged by size:
1679 166 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1273 1608 1611 1713 1716 1875
Size: 1273 335 3 102 3 159
Fragments arranged by size:
1273 335 159 102 3 3
BseYI C'CCAG_C
Cuts at: 0 119 816 929 1875
Size: 119 697 113 946
Fragments arranged by size:
946 697 119 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 1875
Size: 172 1703
BsiHKAI G_wGCw'C
Cuts at: 0 248 1015 1865 1875
Size: 248 767 850 10
Fragments arranged by size:
850 767 248 10
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1513 1875
Size: 1513 362
Bsp1286I G_dGCh'C
Cuts at: 0 248 444 683 1015 1865 1875
Size: 248 196 239 332 850 10
Fragments arranged by size:
850 332 248 239 196 10
BspEI T'CCGG_A
Cuts at: 0 1124 1398 1875
Size: 1124 274 477
Fragments arranged by size:
1124 477 274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 983 1127 1875
Size: 983 144 748
Fragments arranged by size:
983 748 144
BsrBI CCG'CTC
Cuts at: 0 147 1503 1875
Size: 147 1356 372
Fragments arranged by size:
1356 372 147
BsrDI GCAATG_nn'
Cuts at: 0 1247 1875
Size: 1247 628
BsrFI r'CCGG_y
Cuts at: 0 282 450 1875
Size: 282 168 1425
Fragments arranged by size:
1425 282 168
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1651 1875
Size: 1651 224
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1632 1875
Size: 1632 243
BstYI r'GATC_y
Cuts at: 0 1358 1875
Size: 1358 517
BtgI C'CryG_G
Cuts at: 0 140 1424 1875
Size: 140 1284 451
Fragments arranged by size:
1284 451 140
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1166 1201 1875
Size: 1166 35 674
Fragments arranged by size:
1166 674 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 896 1875
Size: 896 979
EaeI y'GGCC_r
Cuts at: 0 404 1214 1421 1875
Size: 404 810 207 454
Fragments arranged by size:
810 454 404 207
EarI CTCTTCn'nnn_
Cuts at: 0 377 1493 1566 1875
Size: 377 1116 73 309
Fragments arranged by size:
1116 377 309 73
EcoICRI GAG'CTC
Cuts at: 0 246 1013 1863 1875
Size: 246 767 850 12
Fragments arranged by size:
850 767 246 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 416 445 491 516 596 612 1006
Size: 262 154 29 46 25 80 16 394
Cuts at: 1006 1179 1291 1305 1396 1768 1875
Size: 173 112 14 91 372 107
Fragments arranged by size:
394 372 262 173 154 112 107 91
80 46 29 25 16 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1378 1468 1875
Size: 1378 90 407
Fragments arranged by size:
1378 407 90
EcoO109I rG'GnC_Cy
Cuts at: 0 425 656 1221 1416 1875
Size: 425 231 565 195 459
Fragments arranged by size:
565 459 425 231 195
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1098 1130 1175 1207 1603 1635 1711 1743
Size: 1098 32 45 32 396 32 76 32
Cuts at: 1743 1875
Size: 132
Fragments arranged by size:
1098 396 132 76 45 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1083 1596 1875
Size: 1083 513 279
Fragments arranged by size:
1083 513 279
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1592 1875
Size: 1592 283
MscI TGG'CCA
Cuts at: 0 406 1423 1875
Size: 406 1017 452
Fragments arranged by size:
1017 452 406
MslI CAynn'nnrTG
Cuts at: 0 800 1105 1162 1630 1875
Size: 800 305 57 468 245
Fragments arranged by size:
800 468 305 245 57
MspA1I CmG'CkG
Cuts at: 0 21 123 1173 1199 1875
Size: 21 102 1050 26 676
Fragments arranged by size:
1050 676 102 26 21
NaeI GCC'GGC
Cuts at: 0 452 1875
Size: 452 1423
NcoI C'CATG_G
Cuts at: 0 140 1875
Size: 140 1735
NgoMIV G'CCGG_C
Cuts at: 0 450 1875
Size: 450 1425
NspI r_CATG'y
Cuts at: 0 198 1875
Size: 198 1677
PasI CC'CwG_GG
Cuts at: 0 421 1640 1875
Size: 421 1219 235
Fragments arranged by size:
1219 421 235
PfoI T'CCnGG_A
Cuts at: 0 738 1321 1875
Size: 738 583 554
Fragments arranged by size:
738 583 554
PpuMI rG'GwC_Cy
Cuts at: 0 425 656 1221 1875
Size: 425 231 565 654
Fragments arranged by size:
654 565 425 231
PstI C_TGCA'G
Cuts at: 0 896 1469 1875
Size: 896 573 406
Fragments arranged by size:
896 573 406
PvuII CAG'CTG
Cuts at: 0 21 123 1173 1199 1875
Size: 21 102 1050 26 676
Fragments arranged by size:
1050 676 102 26 21
SacI G_AGCT'C
Cuts at: 0 248 1015 1865 1875
Size: 248 767 850 10
Fragments arranged by size:
850 767 248 10
SapI GCTCTTCn'nnn_
Cuts at: 0 1493 1566 1875
Size: 1493 73 309
Fragments arranged by size:
1493 309 73
SbfI CC_TGCA'GG
Cuts at: 0 1469 1875
Size: 1469 406
SfcI C'TryA_G
Cuts at: 0 672 880 892 1465 1875
Size: 672 208 12 573 410
Fragments arranged by size:
672 573 410 208 12
SmlI C'TyrA_G
Cuts at: 0 492 1875
Size: 492 1383
SphI G_CATG'C
Cuts at: 0 198 1875
Size: 198 1677
SspI AAT'ATT
Cuts at: 0 1824 1875
Size: 1824 51
StuI AGG'CCT
Cuts at: 0 1735 1875
Size: 1735 140
StyI C'CwwG_G
Cuts at: 0 140 1531 1875
Size: 140 1391 344
Fragments arranged by size:
1391 344 140
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 21 1073 1875
Size: 21 1052 802
Fragments arranged by size:
1052 802 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 31 63 166 198 217 249 528 560
Size: 31 32 103 32 19 32 279 32
Cuts at: 560 1875
Size: 1315
Fragments arranged by size:
1315 279 103 32 32 32 32 31
19
Tth111I GACn'n_nGTC
Cuts at: 0 607 1875
Size: 607 1268
XmnI GAAnn'nnTTC
Cuts at: 0 697 1875
Size: 697 1178
ZraI GAC'GTC
Cuts at: 0 669 1130 1875
Size: 669 461 745
Fragments arranged by size:
745 669 461
Enzymes that do cut:
AarI AatII AccI AcuI AlwNI ApoI BanI BanII
BbsI BbvCI BclI BglII BlpI Bme1580I BmgBI BplI
BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BstAPI BstXI BstYI BtgI CspCI DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I Hin4I HincII
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PasI PfoI PpuMI PstI PvuII SacI SapI SbfI
SfcI SmlI SphI SspI StuI StyI TaqII TstI
Tth111I XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BcgI BciVI BfrBI BglI
BmrI BmtI BsaI BsaBI BsiEI BsiWI BsmI BspHI
BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
BtsI ClaI DraI DraIII EagI EciI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI NarI NdeI NheI NotI NruI
NsiI PacI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SwaI TatI XbaI XcmI XhoI
XmaI