(Circular) (Six-base) MAPSORT of: pFN21AB8500.seq Check: 8236 from: 1 to: 7291 pFN21AB8500 7291 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2083 2083 Size: 7291 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4353 5101 278 Size: 53 83 186 936 2817 748 2468 Fragments arranged by size: 2817 2468 936 748 186 83 53 AccI GT'mk_AC Cuts at: 1937 2931 4634 1937 Size: 994 1703 4594 Fragments arranged by size: 4594 1703 994 Acc65I G'GTAC_C Cuts at: 4612 4612 Size: 7291 AclI AA'CG_TT Cuts at: 1563 4590 5418 5791 1563 Size: 3027 828 373 3063 Fragments arranged by size: 3063 3027 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3363 5354 6738 7006 1632 Size: 1731 1991 1384 268 1917 Fragments arranged by size: 1991 1917 1731 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2177 820 Size: 197 1160 5934 Fragments arranged by size: 5934 1160 197 AflIII A'CryG_T Cuts at: 1203 2937 3246 3598 3801 6190 7167 1203 Size: 1734 309 352 203 2389 977 1327 Fragments arranged by size: 2389 1734 1327 977 352 309 203 AgeI A'CCGG_T Cuts at: 6122 6122 Size: 7291 AhdI GACnn_n'nnGTC Cuts at: 6020 6020 Size: 7291 AleI CACnn'nnGTG Cuts at: 1166 3503 1166 Size: 2337 4954 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2926 4440 1184 Size: 1742 1514 4035 Fragments arranged by size: 4035 1742 1514 AlwNI CAG_nnn'CTG Cuts at: 2036 3133 3546 4289 4437 5034 6606 2036 Size: 1097 413 743 148 597 1572 2721 Fragments arranged by size: 2721 1572 1097 743 597 413 148 ApaLI G'TGCA_C Cuts at: 2349 5348 6504 2349 Size: 2999 1156 3136 Fragments arranged by size: 3136 2999 1156 ApoI r'AATT_y Cuts at: 1493 2519 3030 3686 3753 4600 4826 4978 7087 Size: 1026 511 656 67 847 226 152 2109 Cuts at: 7087 1493 Size: 1697 Fragments arranged by size: 2109 1697 1026 847 656 511 226 152 67 AseI AT'TA_AT Cuts at: 160 1032 3837 5845 160 Size: 872 2805 2008 1606 Fragments arranged by size: 2805 2008 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7291 AvaI C'yCGr_G Cuts at: 1870 1943 2023 3484 4407 4616 1870 Size: 73 80 1461 923 209 4545 Fragments arranged by size: 4545 1461 923 209 80 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2939 2972 2939 Size: 33 7258 BamHI G'GATC_C Cuts at: 4621 4988 4621 Size: 367 6924 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2153 3383 4612 6067 618 Size: 325 217 627 366 1230 1229 1455 1842 Fragments arranged by size: 1842 1455 1230 1229 627 366 325 217 BanII G_rGCy'C Cuts at: 1391 2024 2342 3341 3590 4610 1391 Size: 633 318 999 249 1020 4072 Fragments arranged by size: 4072 1020 999 633 318 249 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3360 928 Size: 971 1461 4859 Fragments arranged by size: 4859 1461 971 BbvCI CC'TCA_GC Cuts at: 3702 3702 Size: 7291 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4259 4293 5482 5516 1903 Size: 34 2322 34 1189 34 3678 Fragments arranged by size: 3678 2322 1189 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2967 5183 6393 2967 Size: 2216 1210 3865 Fragments arranged by size: 3865 2216 1210 BclI T'GATC_A Cuts at: 1548 2416 4494 1548 Size: 868 2078 4345 Fragments arranged by size: 4345 2078 868 BfrBI ATG'CAT Cuts at: 2573 3166 2573 Size: 593 6698 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5902 136 Size: 107 122 71 631 605 4230 1525 Fragments arranged by size: 4230 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3410 7286 3410 Size: 3876 3415 BlpI GC'TnA_GC Cuts at: 4373 4708 4373 Size: 335 6956 Bme1580I G_kGCm'C Cuts at: 1790 2353 3492 3855 5352 6508 1790 Size: 563 1139 363 1497 1156 2573 Fragments arranged by size: 2573 1497 1156 1139 563 363 BmgBI CAC'GTC Cuts at: 1318 3247 1318 Size: 1929 5362 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3441 5022 5980 Size: 416 53 83 399 24 2024 1581 958 Cuts at: 5980 442 Size: 1753 Fragments arranged by size: 2024 1753 1581 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7291 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3499 3499 Size: 7291 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3000 3249 3306 3987 4142 4266 4414 5951 Size: 1761 249 57 681 155 124 148 1537 Cuts at: 5951 1239 Size: 2579 Fragments arranged by size: 2579 1761 1537 681 249 155 148 124 57 Bpu10I CC'TnA_GC Cuts at: 2040 3702 2040 Size: 1662 5629 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5416 6281 6579 5416 Size: 865 298 6128 Fragments arranged by size: 6128 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5954 882 Size: 617 4455 2219 Fragments arranged by size: 4455 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5082 493 Size: 711 3878 2702 Fragments arranged by size: 3878 2702 711 BsaBI GATnn'nnATC Cuts at: 7285 7285 Size: 7291 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3402 4350 5098 5480 Size: 53 83 186 936 1869 948 748 382 Cuts at: 5480 6115 275 Size: 635 1451 Fragments arranged by size: 1869 1451 948 936 748 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3677 5724 6122 6396 6543 6954 3677 Size: 2047 398 274 147 411 4014 Fragments arranged by size: 4014 2047 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7066 Fragments arranged by size: 7066 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7291 BseYI C'CCAG_C Cuts at: 2666 2899 6494 6990 2666 Size: 233 3595 496 2967 Fragments arranged by size: 3595 2967 496 233 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2501 1163 Size: 573 765 5953 Fragments arranged by size: 5953 765 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4741 5502 6530 1540 Size: 275 186 2740 761 1028 2301 Fragments arranged by size: 2740 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2024 2342 2353 4158 5352 5437 6508 2024 Size: 318 11 1805 1194 85 1071 2807 Fragments arranged by size: 2807 1805 1194 1071 318 85 11 BsmI GAATG_Cn' Cuts at: 2999 4802 4895 2999 Size: 1803 93 5395 Fragments arranged by size: 5395 1803 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2342 2353 3341 3492 3590 3855 Size: 399 234 318 11 988 151 98 265 Cuts at: 3855 4158 4610 5352 5437 6508 1391 Size: 303 452 742 85 1071 2174 Fragments arranged by size: 2174 1071 988 742 452 399 318 303 265 234 151 98 85 11 BspEI T'CCGG_A Cuts at: 6954 6954 Size: 7291 BspHI T'CATG_A Cuts at: 3904 5180 6910 3904 Size: 1276 1730 4285 Fragments arranged by size: 4285 1730 1276 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2044 2083 2384 4646 844 Size: 1200 39 301 2262 3489 Fragments arranged by size: 3489 2262 1200 301 39 BsrBI CCG'CTC Cuts at: 1394 1823 3467 5178 1394 Size: 429 1644 1711 3507 Fragments arranged by size: 3507 1711 1644 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5786 5960 65 Size: 1183 4538 174 1396 Fragments arranged by size: 4538 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2883 4398 5935 6122 1700 Size: 110 1073 1515 1537 187 2869 Fragments arranged by size: 2869 1537 1515 1073 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7291 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7291 BssSI C'ACGA_G Cuts at: 2918 5351 6363 2918 Size: 2433 1012 3846 Fragments arranged by size: 3846 2433 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3034 3133 3546 4153 794 Size: 2240 99 413 607 3932 Fragments arranged by size: 3932 2240 607 413 99 BstBI TT'CG_AA Cuts at: 2244 6095 2244 Size: 3851 3440 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7291 BstYI r'GATC_y Cuts at: 1972 2194 3410 4621 4988 5373 5390 6842 6928 Size: 222 1216 1211 367 385 17 1452 86 Cuts at: 6928 6940 7286 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1216 1211 385 367 346 222 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3189 3504 4110 4537 513 Size: 552 939 1185 315 606 427 3267 Fragments arranged by size: 3267 1185 939 606 552 427 315 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2373 4546 487 Size: 683 56 1147 2173 3232 Fragments arranged by size: 3232 2173 1147 683 56 BtsI GCAGTG_nn' Cuts at: 2112 3131 3432 3544 4807 5601 5621 2112 Size: 1019 301 112 1263 794 20 3782 Fragments arranged by size: 3782 1263 1019 794 301 112 20 ClaI AT'CG_AT Cuts at: 1554 4269 1554 Size: 2715 4576 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2146 2181 2203 2238 529 Size: 35 1582 35 22 35 5582 Fragments arranged by size: 5582 1582 35 35 35 22 DraI TTT'AAA Cuts at: 3069 3742 4595 3069 Size: 673 853 5765 Fragments arranged by size: 5765 853 673 DraIII CAC_nnn'GTG Cuts at: 2348 4153 2348 Size: 1805 5486 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3401 6298 809 Size: 507 2085 2897 1802 Fragments arranged by size: 2897 2085 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3564 4738 5627 Size: 54 1425 50 275 18 1734 1174 889 Cuts at: 5627 7256 8 Size: 1629 43 Fragments arranged by size: 1734 1629 1425 1174 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4738 1537 Size: 275 2926 4090 Fragments arranged by size: 4090 2926 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2757 3285 5221 1353 Size: 1404 528 1936 3423 Fragments arranged by size: 3423 1936 1404 528 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5878 6252 6398 387 Size: 1064 138 4289 374 146 1280 Fragments arranged by size: 4289 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2022 2340 2022 Size: 318 6973 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3000 3249 3306 3363 3987 4142 4266 Size: 393 1368 249 57 57 624 155 124 Cuts at: 4266 4414 5354 5951 6738 7006 1239 Size: 148 940 597 787 268 1524 Fragments arranged by size: 1524 1368 940 787 624 597 393 268 249 155 148 124 57 57 EcoNI CCTnn'n_nnAGG Cuts at: 2300 2300 Size: 7291 EcoRI G'AATT_C Cuts at: 4600 4978 4600 Size: 378 6913 EcoRV GAT'ATC Cuts at: 3004 4087 3004 Size: 1083 6208 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3773 4144 4270 3773 Size: 371 126 6794 Fragments arranged by size: 6794 371 126 FspI TGC'GCA Cuts at: 5797 5797 Size: 7291 HaeII r_GCGC'y Cuts at: 1678 3623 6438 1678 Size: 1945 2815 2531 Fragments arranged by size: 2815 2531 1945 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2051 2083 2401 2433 3421 Size: 32 135 32 479 32 318 32 988 Cuts at: 3421 3453 3572 3604 4213 4245 5932 5964 6006 Size: 32 119 32 609 32 1687 32 42 Cuts at: 6006 6038 1373 Size: 32 2626 Fragments arranged by size: 2626 1687 988 609 479 318 135 119 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3106 4635 4881 669 Size: 988 281 1168 1529 246 3079 Fragments arranged by size: 3079 1529 1168 988 281 246 HindIII A'AGCT_T Cuts at: 748 2174 3050 748 Size: 1426 876 4989 Fragments arranged by size: 4989 1426 876 HpaI GTT'AAC Cuts at: 4881 4881 Size: 7291 KpnI G_GTAC'C Cuts at: 4616 4616 Size: 7291 MfeI C'AATT_G Cuts at: 4890 4890 Size: 7291 MluI A'CGCG_T Cuts at: 7167 7167 Size: 7291 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2377 2824 6405 6589 1305 Size: 1072 447 3581 184 2007 Fragments arranged by size: 3581 2007 1072 447 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5469 Fragments arranged by size: 5469 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3503 4502 5249 5608 5767 518 Size: 648 2337 999 747 359 159 2042 Fragments arranged by size: 2337 2042 999 747 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2737 3173 4707 5384 6532 6777 1606 Size: 1131 436 1534 677 1148 245 2120 Fragments arranged by size: 2120 1534 1148 1131 677 436 245 NaeI GCC'GGC Cuts at: 4400 4400 Size: 7291 NcoI C'CATG_G Cuts at: 513 1065 2004 3189 4537 513 Size: 552 939 1185 1348 3267 Fragments arranged by size: 3267 1348 1185 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7291 NgoMIV G'CCGG_C Cuts at: 4398 4398 Size: 7291 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7291 NotI GC'GGCC_GC Cuts at: 4738 4738 Size: 7291 NsiI A_TGCA'T Cuts at: 2575 3168 2575 Size: 593 6698 NspI r_CATG'y Cuts at: 1132 1229 2573 2941 3170 3602 3805 4109 4649 Size: 97 1344 368 229 432 203 304 540 Cuts at: 4649 6194 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 1344 540 432 368 304 229 203 97 PasI CC'CwG_GG Cuts at: 1348 3575 4385 1348 Size: 2227 810 4254 Fragments arranged by size: 4254 2227 810 PciI A'CATG_T Cuts at: 2937 3598 3801 6190 2937 Size: 661 203 2389 4038 Fragments arranged by size: 4038 2389 661 203 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7291 PfoI T'CCnGG_A Cuts at: 3964 3964 Size: 7291 PmeI GTTT'AAAC Cuts at: 4595 4595 Size: 7291 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5360 5392 6873 6905 5360 Size: 32 1481 32 5746 Fragments arranged by size: 5746 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2471 3774 1613 Size: 858 1303 5130 Fragments arranged by size: 5130 1303 858 PsiI TTA'TAA Cuts at: 4064 4861 4064 Size: 797 6494 PspXI vC'TCGA_Gb Cuts at: 2023 2023 Size: 7291 PstI C_TGCA'G Cuts at: 830 2915 3551 4143 4643 830 Size: 2085 636 592 500 3478 Fragments arranged by size: 3478 2085 636 592 500 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7291 PvuII CAG'CTG Cuts at: 2737 2737 Size: 7291 SacI G_AGCT'C Cuts at: 2024 2342 2024 Size: 318 6973 SalI G'TCGA_C Cuts at: 1936 4633 1936 Size: 2697 4594 SbfI CC_TGCA'GG Cuts at: 4643 4643 Size: 7291 ScaI AGT'ACT Cuts at: 5539 7251 5539 Size: 1712 5579 SfcI C'TryA_G Cuts at: 826 1045 2911 3547 4139 4639 5774 6455 6646 Size: 219 1866 636 592 500 1135 681 191 Cuts at: 6646 826 Size: 1471 Fragments arranged by size: 1866 1471 1135 681 636 592 500 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7291 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7291 SmaI CCC'GGG Cuts at: 1872 4618 1872 Size: 2746 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2023 2177 5395 6296 6558 820 Size: 197 926 80 154 3218 901 262 1553 Fragments arranged by size: 3218 1553 926 901 262 197 154 80 SnaBI TAC'GTA Cuts at: 493 5082 493 Size: 4589 2702 SpeI A'CTAG_T Cuts at: 152 4319 152 Size: 4167 3124 SphI G_CATG'C Cuts at: 1132 3170 4109 4649 1132 Size: 2038 939 540 3774 Fragments arranged by size: 3774 2038 939 540 SspI AAT'ATT Cuts at: 5 52 2164 2533 4239 5215 5 Size: 47 2112 369 1706 976 2081 Fragments arranged by size: 2112 2081 1706 976 369 47 StuI AGG'CCT Cuts at: 1517 3272 1517 Size: 1755 5536 StyI C'CwwG_G Cuts at: 513 1065 2004 3189 4537 4730 513 Size: 552 939 1185 1348 193 3074 Fragments arranged by size: 3074 1348 1185 939 552 193 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4514 4562 5318 5335 5488 5673 6118 Size: 1255 2934 48 756 17 153 185 445 Cuts at: 6118 325 Size: 1498 Fragments arranged by size: 2934 1498 1255 756 445 185 153 48 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2027 5537 7249 Size: 274 80 33 51 1443 50 3510 1712 Cuts at: 7249 96 Size: 138 Fragments arranged by size: 3510 1712 1443 274 138 80 51 50 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7251 7283 7251 Size: 32 7259 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7291 XbaI T'CTAG_A Cuts at: 4627 4627 Size: 7291 XhoI C'TCGA_G Cuts at: 1943 2023 1943 Size: 80 7211 XmaI C'CCGG_G Cuts at: 1870 4616 1870 Size: 2746 4545 XmnI GAAnn'nnTTC Cuts at: 2851 3753 4215 5420 2851 Size: 902 462 1205 4722 Fragments arranged by size: 4722 1205 902 462 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4351 5099 276 Size: 53 83 186 936 2817 748 2468 Fragments arranged by size: 2817 2468 936 748 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoO109I FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI