Restriction map for
pFN21AB9948
##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI ApaI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BbvCI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraIII Eam1105I EcoO65I FbaI FseI
FspI HgaI KpnI MaeI MamI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NruI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI SexAI
SfiI SgrAI SmaI SmlI SpeI
Sse8387I SspBI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AatII 511 GACGTC GACGT^C
AccII 475 CGCG CG^CG
AflIII 320 ACACGT A^C[GA][CT]GT
AlwNI 150 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 75 CCTCAGG CC^T[ACGT]AGG
Aor51HI 422 AGCGCT AGC^GCT
Asp700I 756 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1528 CCCGAG C^[CT]CG[GA]G
BalI 1706 TGGCCA TGG^CCA
BbeI 70 GGCGCC GGCGC^C
BcgI 1530 CGAGATAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 446 GCCTGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 1001 GAGACC GGTCTC(1/5)
BseRI 40 GAGGAG GAGGAG(10/8)
BsgI 156 CTGCAC GTGCAG(16/14)
BsoBI 1528 CCCGAG C^[CT]CG[GA]G
BspMI 400 ACCTGC ACCTGC(4/8)
BsrFI 1307 GCCGGT [GA]^CCGG[CT]
BstBI 1966 TTCGAA TT^CGAA
BstUI 475 CGCG CG^CG
Bsu36I 75 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1307 GCCGGT [GA]^CCGG[CT]
DraI 1998 TTTAAA TTT^AAA
DraII 944 AGGTCCC [GA]G^G[ACGT]CC[CT]
EarI 1645 CTCTTC CTCTTC(1/4)
Eco47III 422 AGCGCT AGC^GCT
Eco81I 75 CCTCAGG CC^T[ACGT]AGG
EcoNI 1116 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 944 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1963 GAATTC G^AATTC
EcoRV 1777 GATATC GAT^ATC
EcoT22I 1790 ATGCAT ATGCA^T
HincII 1984 GTTAAC GT[CT]^[GA]AC
HindII 1984 GTTAAC GT[CT]^[GA]AC
HindIII 1859 AAGCTT A^AGCTT
HpaI 1984 GTTAAC GTT^AAC
KasI 70 GGCGCC G^GCGCC
Ksp632I 1645 CTCTTC CTCTTC(1/4)
KspI 474 CCGCGG CCGC^GG
MluNI 1706 TGGCCA TGG^CCA
MscI 1706 TGGCCA TGG^CCA
MvnI 475 CGCG CG^CG
NarI 70 GGCGCC GG^CGCC
NcoI 1235 CCATGG C^CATGG
NotI 476 GCGGCCGC GC^GGCCGC
NsiI 1790 ATGCAT ATGCA^T
NspV 1966 TTCGAA TT^CGAA
PflMI 1230 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 944 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 268 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 688 CAGCTG CAG^CTG
SacI 1096 GAGCTC GAGCT^C
SacII 474 CCGCGG CCGC^GG
ScaI 851 AGTACT AGT^ACT
SfoI 70 GGCGCC GGC^GCC
SfuI 1966 TTCGAA TT^CGAA
SnaBI 583 TACGTA TAC^GTA
SphI 1788 GCATGC GCATG^C
SspI 1948 AATATT AAT^ATT
Van91I 1230 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1694 CCAAGGAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 756 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 70 GGCGCC G[GA]^CG[CT]C
511 GACGTC
BanII 1096 GAGCTC G[GA]GC[CT]^C
1318 GAGCCC
BbsI 630 GAAGAC GAAGAC(2/6)
713 GAAGAC
BcnI 9 CCCGG CC^[GC]GG
948 CCCGG
BpmI 327 CTCCAG CTGGAG(16/14)
1511 CTCCAG
BpuAI 630 GAAGAC GAAGAC(2/6)
713 GAAGAC
BsaHI 70 GGCGCC G[GA]^CG[CT]C
511 GACGTC
BsaWI 1004 ACCGGA [AT]^CCGG[AT]
1799 TCCGGT
BstAPI 149 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1342 GCAGAAATTGC
DrdI 505 GACAGGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1930 GACCAGGAAGTC
EagI 477 CGGCCG C^GGCCG
1305 CGGCCG
EclXI 477 CGGCCG C^GGCCG
1305 CGGCCG
Eco52I 477 CGGCCG C^GGCCG
1305 CGGCCG
Hin1I 70 GGCGCC G[GA]^CG[CT]C
511 GACGTC
MslI 399 CACCTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1158 CACTATGGTG
MspA1I 474 CCGCGG C[AC]G^C[GT]G
688 CAGCTG
NciI 9 CCCGG CC^[GC]GG
948 CCCGG
NspI 1457 GCATGT [GA]CATG^[CT]
1788 GCATGC
PleI 371 GACTC GAGTC(4/5)
1583 GAGTC
PstI 147 CTGCAG CTGCA^G
1340 CTGCAG
SfcI 147 CTGCAG C^T[GA][CT]AG
1340 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI ApaLI ApoI AspHI AvaII
BanI BbvI BmyI BsaAI BsaJI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII