(Circular) (Six-base) MAPSORT of: pFN21AB9948.seq Check: 9238 from: 1 to: 6724 pFN21AB9948 6724 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2414 2414 Size: 6724 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2520 4534 278 Size: 53 83 186 936 984 2014 2468 Fragments arranged by size: 2468 2014 984 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4067 1937 Size: 2130 4594 Acc65I G'GTAC_C Cuts at: 4045 4045 Size: 6724 AclI AA'CG_TT Cuts at: 1563 4851 5224 1563 Size: 3288 373 3063 Fragments arranged by size: 3288 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2786 3143 3789 4787 6171 6439 1632 Size: 1154 357 646 998 1384 268 1917 Fragments arranged by size: 1917 1384 1154 998 646 357 268 AfeI AGC'GCT Cuts at: 2429 2429 Size: 6724 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6527 AflIII A'CryG_T Cuts at: 1203 2325 5623 6600 1203 Size: 1122 3298 977 1327 Fragments arranged by size: 3298 1327 1122 977 AgeI A'CCGG_T Cuts at: 5555 5555 Size: 6724 AhdI GACnn_n'nnGTC Cuts at: 5453 5453 Size: 6724 AleI CACnn'nnGTG Cuts at: 1166 3167 1166 Size: 2001 4723 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3089 1184 Size: 1905 4819 AlwNI CAG_nnn'CTG Cuts at: 2160 4467 6039 2160 Size: 2307 1572 2845 Fragments arranged by size: 2845 2307 1572 ApaLI G'TGCA_C Cuts at: 2225 2420 3160 4781 5937 2225 Size: 195 740 1621 1156 3012 Fragments arranged by size: 3012 1621 1156 740 195 ApoI r'AATT_y Cuts at: 1493 2117 2243 2915 2984 3968 3998 4033 4259 Size: 624 126 672 69 984 30 35 226 Cuts at: 4259 4411 6520 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 984 672 624 226 152 126 69 35 30 AseI AT'TA_AT Cuts at: 160 1032 5278 160 Size: 872 4246 1606 Fragments arranged by size: 4246 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6724 AvaI C'yCGr_G Cuts at: 1870 1943 3533 4049 1870 Size: 73 1590 516 4545 Fragments arranged by size: 4545 1590 516 73 BamHI G'GATC_C Cuts at: 4054 4421 4054 Size: 367 6357 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2075 2235 3619 4045 5500 Size: 325 217 627 288 160 1384 426 1455 Cuts at: 5500 618 Size: 1842 Fragments arranged by size: 1842 1455 1384 627 426 325 288 217 160 BanII G_rGCy'C Cuts at: 1391 3105 3327 4043 1391 Size: 1714 222 716 4072 Fragments arranged by size: 4072 1714 716 222 BbeI G_GCGC'C Cuts at: 2079 2079 Size: 6724 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2642 2725 928 Size: 971 743 83 4927 Fragments arranged by size: 4927 971 743 83 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3524 3558 4915 4949 1903 Size: 34 1587 34 1357 34 3678 Fragments arranged by size: 3678 1587 1357 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4616 5826 4616 Size: 1210 5514 BclI T'GATC_A Cuts at: 1548 1548 Size: 6724 BfrBI ATG'CAT Cuts at: 3797 3797 Size: 6724 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2457 5335 136 Size: 107 122 71 631 605 785 2878 1525 Fragments arranged by size: 2878 1525 785 631 605 122 107 71 BglII A'GATC_T Cuts at: 6719 6719 Size: 6724 BlpI GC'TnA_GC Cuts at: 4141 4141 Size: 6724 Bme1580I G_kGCm'C Cuts at: 1790 2229 2424 3164 4785 5941 1790 Size: 439 195 740 1621 1156 2573 Fragments arranged by size: 2573 1621 1156 740 439 195 BmgBI CAC'GTC Cuts at: 1318 3154 1318 Size: 1836 4888 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4455 5413 442 Size: 416 53 83 399 24 3038 958 1753 Fragments arranged by size: 3038 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6724 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2317 3501 5384 1239 Size: 1078 1184 1883 2579 Fragments arranged by size: 2579 1883 1184 1078 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4849 5714 6012 4849 Size: 865 298 5561 Fragments arranged by size: 5561 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3000 5387 882 Size: 617 1501 2387 2219 Fragments arranged by size: 2387 2219 1501 617 BsaAI yAC'GTr Cuts at: 493 1204 2328 2590 3271 4515 493 Size: 711 1124 262 681 1244 2702 Fragments arranged by size: 2702 1244 1124 711 681 262 BsaBI GATnn'nnATC Cuts at: 6718 6718 Size: 6724 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2076 2517 4531 4913 Size: 53 83 186 936 543 441 2014 382 Cuts at: 4913 5548 275 Size: 635 1451 Fragments arranged by size: 2014 1451 936 635 543 441 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3009 3804 5157 5555 5829 5976 6387 3009 Size: 795 1353 398 274 147 411 3346 Fragments arranged by size: 3346 1353 795 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2315 2345 2963 2993 1178 Size: 30 165 30 912 30 618 30 4909 Fragments arranged by size: 4909 912 618 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2060 1186 Size: 874 5850 BseYI C'CCAG_C Cuts at: 2447 3046 3216 3611 5927 6423 2447 Size: 599 170 395 2316 496 2748 Fragments arranged by size: 2748 2316 599 496 395 170 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2146 1163 Size: 573 410 5741 Fragments arranged by size: 5741 573 410 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2485 3313 3895 4174 4935 5963 Size: 275 186 484 828 582 279 761 1028 Cuts at: 5963 1540 Size: 2301 Fragments arranged by size: 2301 1028 828 761 582 484 279 275 186 BsiHKAI G_wGCw'C Cuts at: 2054 2229 2424 3105 3164 4785 4870 5941 2054 Size: 175 195 681 59 1621 85 1071 2837 Fragments arranged by size: 2837 1621 1071 681 195 175 85 59 BsmI GAATG_Cn' Cuts at: 4235 4328 4235 Size: 93 6631 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2229 2424 3105 3164 3327 4043 Size: 399 264 175 195 681 59 163 716 Cuts at: 4043 4785 4870 5941 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 716 681 399 264 195 175 163 85 59 BspEI T'CCGG_A Cuts at: 6387 6387 Size: 6724 BspHI T'CATG_A Cuts at: 4613 6343 4613 Size: 1730 4994 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2414 4079 844 Size: 1570 1665 3489 Fragments arranged by size: 3489 1665 1570 BsrBI CCG'CTC Cuts at: 1394 1823 4611 1394 Size: 429 2788 3507 Fragments arranged by size: 3507 2788 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5219 5393 65 Size: 1183 3971 174 1396 Fragments arranged by size: 3971 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3312 5368 5555 1700 Size: 110 1502 2056 187 2869 Fragments arranged by size: 2869 2056 1502 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6724 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6724 BssSI C'ACGA_G Cuts at: 4784 5796 4784 Size: 1012 5712 BstAPI GCAn_nnn'nTGC Cuts at: 794 2160 3353 794 Size: 1366 1193 4165 Fragments arranged by size: 4165 1366 1193 BstBI TT'CG_AA Cuts at: 3972 5528 3972 Size: 1556 5168 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6724 BstYI r'GATC_y Cuts at: 1972 2319 2581 2774 3117 3840 4054 4421 4806 Size: 347 262 193 343 723 214 367 385 Cuts at: 4806 4823 6275 6361 6373 6719 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 723 385 367 347 346 343 262 214 193 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2081 2081 Size: 6724 BtgI C'CryG_G Cuts at: 513 1065 2004 2479 3240 3567 513 Size: 552 939 475 761 327 3670 Fragments arranged by size: 3670 939 761 552 475 327 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3511 487 Size: 683 56 2285 3700 Fragments arranged by size: 3700 2285 683 56 BtsI GCAGTG_nn' Cuts at: 2158 2363 4240 5034 5054 2158 Size: 205 1877 794 20 3828 Fragments arranged by size: 3828 1877 794 205 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6724 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6689 DraI TTT'AAA Cuts at: 4005 4028 4005 Size: 23 6701 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2516 3941 5731 809 Size: 507 1200 1425 1790 1802 Fragments arranged by size: 1802 1790 1425 1200 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2482 3310 3711 Size: 54 1425 50 275 18 652 828 401 Cuts at: 3711 4171 5060 6689 8 Size: 460 889 1629 43 Fragments arranged by size: 1629 1425 889 828 652 460 401 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2482 3310 4171 1537 Size: 275 670 828 861 4090 Fragments arranged by size: 4090 861 828 670 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3656 4654 1353 Size: 2303 998 3423 Fragments arranged by size: 3423 2303 998 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5311 5685 5831 387 Size: 1064 138 3722 374 146 1280 Fragments arranged by size: 3722 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3103 3103 Size: 6724 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2317 2786 3143 3501 3789 4787 5384 Size: 393 685 469 357 358 288 998 597 Cuts at: 5384 6171 6439 1239 Size: 787 268 1524 Fragments arranged by size: 1524 998 787 685 597 469 393 358 357 288 268 EcoNI CCTnn'n_nnAGG Cuts at: 3125 3125 Size: 6724 EcoO109I rG'GnC_Cy Cuts at: 2950 2950 Size: 6724 EcoRI G'AATT_C Cuts at: 3968 4033 4411 3968 Size: 65 378 6281 Fragments arranged by size: 6281 378 65 EcoRV GAT'ATC Cuts at: 3784 3784 Size: 6724 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3223 3223 Size: 6724 FspI TGC'GCA Cuts at: 5230 5230 Size: 6724 HaeII r_GCGC'y Cuts at: 1678 2079 2271 2431 2479 5871 1678 Size: 401 192 160 48 3392 2531 Fragments arranged by size: 3392 2531 401 192 160 48 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2596 2628 5365 5397 5439 Size: 32 135 32 1024 32 2737 32 42 Cuts at: 5439 5471 1373 Size: 32 2626 Fragments arranged by size: 2737 2626 1024 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3991 4068 4314 669 Size: 988 281 2053 77 246 3079 Fragments arranged by size: 3079 2053 988 281 246 77 HindIII A'AGCT_T Cuts at: 748 3864 748 Size: 3116 3608 HpaI GTT'AAC Cuts at: 3991 4314 3991 Size: 323 6401 KasI G'GCGC_C Cuts at: 2075 2075 Size: 6724 KpnI G_GTAC'C Cuts at: 4049 4049 Size: 6724 MfeI C'AATT_G Cuts at: 4323 4323 Size: 6724 MluI A'CGCG_T Cuts at: 6600 6600 Size: 6724 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5838 6022 1305 Size: 4533 184 2007 Fragments arranged by size: 4533 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3713 10 Size: 54 1425 343 1881 3021 Fragments arranged by size: 3021 1881 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2408 3167 4682 5041 5200 518 Size: 648 1242 759 1515 359 159 2042 Fragments arranged by size: 2042 1515 1242 759 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2481 2695 4140 4817 5965 6210 1606 Size: 875 214 1445 677 1148 245 2120 Fragments arranged by size: 2120 1445 1148 875 677 245 214 NarI GG'CG_CC Cuts at: 2076 2076 Size: 6724 NcoI C'CATG_G Cuts at: 513 1065 2004 3240 513 Size: 552 939 1236 3997 Fragments arranged by size: 3997 1236 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6724 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6724 NotI GC'GGCC_GC Cuts at: 2482 4171 2482 Size: 1689 5035 NsiI A_TGCA'T Cuts at: 3799 3799 Size: 6724 NspI r_CATG'y Cuts at: 1132 1229 3466 3797 4082 5627 1132 Size: 97 2237 331 285 1545 2229 Fragments arranged by size: 2237 2229 1545 331 285 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6724 PciI A'CATG_T Cuts at: 5623 5623 Size: 6724 PflMI CCAn_nnn'nTGG Cuts at: 1108 3241 1108 Size: 2133 4591 PfoI T'CCnGG_A Cuts at: 2952 2952 Size: 6724 PmeI GTTT'AAAC Cuts at: 4028 4028 Size: 6724 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4793 4825 6306 6338 4793 Size: 32 1481 32 5179 Fragments arranged by size: 5179 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2950 2950 Size: 6724 PshAI GACnn'nnGTC Cuts at: 1613 2277 1613 Size: 664 6060 PsiI TTA'TAA Cuts at: 3855 4294 3855 Size: 439 6285 PstI C_TGCA'G Cuts at: 830 2156 3349 4076 830 Size: 1326 1193 727 3478 Fragments arranged by size: 3478 1326 1193 727 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6724 PvuII CAG'CTG Cuts at: 2695 2695 Size: 6724 SacI G_AGCT'C Cuts at: 3105 3105 Size: 6724 SacII CC_GC'GG Cuts at: 2482 2482 Size: 6724 SalI G'TCGA_C Cuts at: 1936 4066 1936 Size: 2130 4594 SbfI CC_TGCA'GG Cuts at: 4076 4076 Size: 6724 ScaI AGT'ACT Cuts at: 2858 4972 6684 2858 Size: 2114 1712 2898 Fragments arranged by size: 2898 2114 1712 SfcI C'TryA_G Cuts at: 826 1045 2152 3345 4072 5207 5888 6079 826 Size: 219 1107 1193 727 1135 681 191 1471 Fragments arranged by size: 1471 1193 1135 1107 727 681 219 191 SfoI GGC'GCC Cuts at: 2077 2077 Size: 6724 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6724 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6724 SmaI CCC'GGG Cuts at: 1872 4051 1872 Size: 2179 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4828 5729 5991 820 Size: 197 926 2885 901 262 1553 Fragments arranged by size: 2885 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2590 4515 493 Size: 2097 1925 2702 Fragments arranged by size: 2702 2097 1925 SpeI A'CTAG_T Cuts at: 152 152 Size: 6724 SphI G_CATG'C Cuts at: 1132 3797 4082 1132 Size: 2665 285 3774 Fragments arranged by size: 3774 2665 285 SspI AAT'ATT Cuts at: 5 52 3955 4648 5 Size: 47 3903 693 2081 Fragments arranged by size: 3903 2081 693 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6724 StyI C'CwwG_G Cuts at: 513 1065 2004 2946 3240 3699 4163 513 Size: 552 939 942 294 459 464 3074 Fragments arranged by size: 3074 942 939 552 464 459 294 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3038 3350 3756 3909 4751 4768 4921 Size: 1255 1458 312 406 153 842 17 153 Cuts at: 4921 5106 5551 325 Size: 185 445 1498 Fragments arranged by size: 1498 1458 1255 842 445 406 312 185 153 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2856 3252 4970 Size: 274 80 33 51 1443 879 396 1718 Cuts at: 4970 6682 96 Size: 1712 138 Fragments arranged by size: 1718 1712 1443 879 396 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2315 2347 2383 2415 3895 3927 6684 6716 2315 Size: 32 36 32 1480 32 2757 32 2323 Fragments arranged by size: 2757 2323 1480 36 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6724 XbaI T'CTAG_A Cuts at: 4060 4060 Size: 6724 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3706 3706 Size: 6724 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6724 XmaI C'CCGG_G Cuts at: 1870 4049 1870 Size: 2179 4545 XmnI GAAnn'nnTTC Cuts at: 2765 4853 2765 Size: 2088 4636 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2518 4532 276 Size: 53 83 186 936 984 2014 2468 Fragments arranged by size: 2468 2014 984 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI