(Circular) (Six-base) MAPSORT of: pFN21AB4203.seq Check: 4807 from: 1 to: 5659 pFN21AB4203 5659 bp With 182 enzymes: SgfI * October 20, 2009 10:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2746 2746 Size: 5659 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2670 3469 278 Size: 53 83 186 936 1134 799 2468 Fragments arranged by size: 2468 1134 936 799 186 83 53 AccI GT'mk_AC Cuts at: 1937 2271 3002 1937 Size: 334 731 4594 Fragments arranged by size: 4594 731 334 Acc65I G'GTAC_C Cuts at: 2980 2980 Size: 5659 AclI AA'CG_TT Cuts at: 1563 3786 4159 1563 Size: 2223 373 3063 Fragments arranged by size: 3063 2223 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2771 3722 5106 5374 1632 Size: 1139 951 1384 268 1917 Fragments arranged by size: 1917 1384 1139 951 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5462 AflIII A'CryG_T Cuts at: 1203 2322 4558 5535 1203 Size: 1119 2236 977 1327 Fragments arranged by size: 2236 1327 1119 977 AgeI A'CCGG_T Cuts at: 4490 4490 Size: 5659 AhdI GACnn_n'nnGTC Cuts at: 4388 4388 Size: 5659 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5659 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5659 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2036 2068 2036 Size: 32 5627 AlwNI CAG_nnn'CTG Cuts at: 2749 2833 3402 4974 2749 Size: 84 569 1572 3434 Fragments arranged by size: 3434 1572 569 84 ApaI G_GGCC'C Cuts at: 2446 2446 Size: 5659 ApaLI G'TGCA_C Cuts at: 3716 4872 3716 Size: 1156 4503 ApoI r'AATT_y Cuts at: 1493 2968 3194 3346 5455 1493 Size: 1475 226 152 2109 1697 Fragments arranged by size: 2109 1697 1475 226 152 AseI AT'TA_AT Cuts at: 160 1032 4213 160 Size: 872 3181 1606 Fragments arranged by size: 3181 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 AvaI C'yCGr_G Cuts at: 1870 1943 2804 2984 1870 Size: 73 861 180 4545 Fragments arranged by size: 4545 861 180 73 BamHI G'GATC_C Cuts at: 2199 2989 3356 2199 Size: 790 367 4502 Fragments arranged by size: 4502 790 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2177 2980 4435 618 Size: 325 217 627 390 803 1455 1842 Fragments arranged by size: 1842 1455 803 627 390 325 217 BanII G_rGCy'C Cuts at: 1391 2343 2446 2978 1391 Size: 952 103 532 4072 Fragments arranged by size: 4072 952 532 103 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2918 2958 928 Size: 971 1019 40 3629 Fragments arranged by size: 3629 1019 971 40 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3850 3884 1903 Size: 34 1913 34 3678 Fragments arranged by size: 3678 1913 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3551 4761 3551 Size: 1210 4449 BclI T'GATC_A Cuts at: 1548 1548 Size: 5659 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4270 136 Size: 107 122 71 631 605 2598 1525 Fragments arranged by size: 2598 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5654 5654 Size: 5659 BlpI GC'TnA_GC Cuts at: 3076 3076 Size: 5659 Bme1580I G_kGCm'C Cuts at: 1790 2305 2446 3720 4876 1790 Size: 515 141 1274 1156 2573 Fragments arranged by size: 2573 1274 1156 515 141 BmgBI CAC'GTC Cuts at: 1318 2323 1318 Size: 1005 4654 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2135 3390 4348 Size: 416 53 83 399 24 718 1255 958 Cuts at: 4348 442 Size: 1753 Fragments arranged by size: 1753 1255 958 718 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5659 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2468 2692 2888 4319 1239 Size: 1229 224 196 1431 2579 Fragments arranged by size: 2579 1431 1229 224 196 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3784 4649 4947 3784 Size: 865 298 4496 Fragments arranged by size: 4496 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4322 882 Size: 617 2823 2219 Fragments arranged by size: 2823 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3450 493 Size: 711 2246 2702 Fragments arranged by size: 2702 2246 711 BsaBI GATnn'nnATC Cuts at: 2702 5653 2702 Size: 2951 2708 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2667 3466 3848 4483 Size: 53 83 186 936 1134 799 382 635 Cuts at: 4483 275 Size: 1451 Fragments arranged by size: 1451 1134 936 799 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2370 4092 4490 4764 4911 5322 2370 Size: 1722 398 274 147 411 2707 Fragments arranged by size: 2707 1722 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2036 2066 2756 2783 2786 Size: 30 165 30 633 30 690 27 3 Cuts at: 2786 2813 1178 Size: 27 4024 Fragments arranged by size: 4024 690 633 165 30 30 30 27 27 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2780 1186 Size: 1594 4065 BseYI C'CCAG_C Cuts at: 2778 2785 4862 5358 2778 Size: 7 2077 496 3079 Fragments arranged by size: 3079 2077 496 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2441 1163 Size: 573 705 4381 Fragments arranged by size: 4381 705 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2271 2794 3109 3870 4898 1540 Size: 275 186 270 523 315 761 1028 2301 Fragments arranged by size: 2301 1028 761 523 315 275 270 186 BsiHKAI G_wGCw'C Cuts at: 2343 2883 3720 3805 4876 2343 Size: 540 837 85 1071 3126 Fragments arranged by size: 3126 1071 837 540 85 BsmI GAATG_Cn' Cuts at: 3170 3263 3170 Size: 93 5566 BsmBI CGTCTCn'nnnn_ Cuts at: 2313 2313 Size: 5659 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2305 2343 2446 2883 2978 3720 3805 Size: 399 515 38 103 437 95 742 85 Cuts at: 3805 4876 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 515 437 399 103 95 85 38 BspEI T'CCGG_A Cuts at: 5322 5322 Size: 5659 BspHI T'CATG_A Cuts at: 3548 5278 3548 Size: 1730 3929 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2746 3014 844 Size: 1902 268 3489 Fragments arranged by size: 3489 1902 268 BsrBI CCG'CTC Cuts at: 1394 1823 2175 3546 1394 Size: 429 352 1371 3507 Fragments arranged by size: 3507 1371 429 352 BsrDI GCAATG_nn' Cuts at: 65 1248 2919 4154 4328 65 Size: 1183 1671 1235 174 1396 Fragments arranged by size: 1671 1396 1235 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2591 4303 4490 1700 Size: 110 781 1712 187 2869 Fragments arranged by size: 2869 1712 781 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5659 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5659 BssSI C'ACGA_G Cuts at: 3719 4731 3719 Size: 1012 4647 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5659 BstBI TT'CG_AA Cuts at: 4463 4463 Size: 5659 BstEII G'GTnAC_C Cuts at: 1184 2110 1184 Size: 926 4733 BstXI CCAn_nnnn'nTGG Cuts at: 2584 2584 Size: 5659 BstYI r'GATC_y Cuts at: 1972 2199 2328 2940 2989 3356 3741 3758 5210 Size: 227 129 612 49 367 385 17 1452 Cuts at: 5210 5296 5308 5654 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 612 385 367 346 227 129 86 49 17 12 Bsu36I CC'TnA_GG Cuts at: 2834 2834 Size: 5659 BtgI C'CryG_G Cuts at: 513 1065 2131 513 Size: 552 1066 4041 Fragments arranged by size: 4041 1066 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4920 Fragments arranged by size: 4920 683 56 BtsI GCAGTG_nn' Cuts at: 2820 3175 3969 3989 2820 Size: 355 794 20 4490 Fragments arranged by size: 4490 794 355 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5659 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5624 DraI TTT'AAA Cuts at: 2963 2963 Size: 5659 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4666 809 Size: 507 3350 1802 Fragments arranged by size: 3350 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2187 2574 2700 Size: 54 1425 50 275 18 357 387 126 Cuts at: 2700 3106 3995 5624 8 Size: 406 889 1629 43 Fragments arranged by size: 1629 1425 889 406 387 357 275 126 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3106 1537 Size: 275 1294 4090 Fragments arranged by size: 4090 1294 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3589 1353 Size: 2236 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4246 4620 4766 387 Size: 1064 138 2657 374 146 1280 Fragments arranged by size: 2657 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2341 2341 Size: 5659 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2468 2692 2771 2888 3722 4319 5106 Size: 393 836 224 79 117 834 597 787 Cuts at: 5106 5374 1239 Size: 268 1524 Fragments arranged by size: 1524 836 834 787 597 393 268 224 117 79 EcoO109I rG'GnC_Cy Cuts at: 2443 2733 2443 Size: 290 5369 EcoRI G'AATT_C Cuts at: 2968 3346 2968 Size: 378 5281 FspI TGC'GCA Cuts at: 2264 4165 2264 Size: 1901 3758 FspAI rTGC'GCAy Cuts at: 2264 2264 Size: 5659 HaeII r_GCGC'y Cuts at: 1678 4806 1678 Size: 3128 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2756 2783 2788 2815 4300 Size: 32 135 32 1184 27 5 27 1485 Cuts at: 4300 4332 4374 4406 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1485 1184 135 42 32 32 32 32 27 27 5 HincII GTy'rAC Cuts at: 669 1657 1938 2272 3003 3249 669 Size: 988 281 334 731 246 3079 Fragments arranged by size: 3079 988 731 334 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5659 HpaI GTT'AAC Cuts at: 3249 3249 Size: 5659 KpnI G_GTAC'C Cuts at: 2984 2984 Size: 5659 MfeI C'AATT_G Cuts at: 3258 3258 Size: 5659 MluI A'CGCG_T Cuts at: 5535 5535 Size: 5659 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2688 4773 4957 1305 Size: 1383 2085 184 2007 Fragments arranged by size: 2085 2007 1383 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2576 2702 10 Size: 54 1425 343 744 126 2967 Fragments arranged by size: 2967 1425 744 343 126 54 MslI CAynn'nnrTG Cuts at: 518 1166 2183 2189 2258 2484 2687 3617 3976 Size: 648 1017 6 69 226 203 930 359 Cuts at: 3976 4135 518 Size: 159 2042 Fragments arranged by size: 2042 1017 930 648 359 226 203 159 69 6 MspA1I CmG'CkG Cuts at: 1606 2749 3075 3752 4900 5145 1606 Size: 1143 326 677 1148 245 2120 Fragments arranged by size: 2120 1148 1143 677 326 245 NaeI GCC'GGC Cuts at: 2593 2593 Size: 5659 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5107 NdeI CA'TA_TG Cuts at: 387 387 Size: 5659 NgoMIV G'CCGG_C Cuts at: 2591 2591 Size: 5659 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5659 NotI GC'GGCC_GC Cuts at: 3106 3106 Size: 5659 NspI r_CATG'y Cuts at: 1132 1229 2263 3017 4562 1132 Size: 97 1034 754 1545 2229 Fragments arranged by size: 2229 1545 1034 754 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5659 PciI A'CATG_T Cuts at: 4558 4558 Size: 5659 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5659 PfoI T'CCnGG_A Cuts at: 2054 2054 Size: 5659 PmeI GTTT'AAAC Cuts at: 2963 2963 Size: 5659 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2036 2068 3728 3760 5241 5273 2036 Size: 32 1660 32 1481 32 2422 Fragments arranged by size: 2422 1660 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2733 2733 Size: 5659 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5659 PsiI TTA'TAA Cuts at: 3229 3229 Size: 5659 PspOMI G'GGCC_C Cuts at: 2442 2442 Size: 5659 PstI C_TGCA'G Cuts at: 830 3011 830 Size: 2181 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5659 PvuII CAG'CTG Cuts at: 2749 2749 Size: 5659 SacI G_AGCT'C Cuts at: 2343 2343 Size: 5659 SalI G'TCGA_C Cuts at: 1936 2270 3001 1936 Size: 334 731 4594 Fragments arranged by size: 4594 731 334 SanDI GG'GwC_CC Cuts at: 2733 2733 Size: 5659 SbfI CC_TGCA'GG Cuts at: 3011 3011 Size: 5659 ScaI AGT'ACT Cuts at: 3907 5619 3907 Size: 1712 3947 SfcI C'TryA_G Cuts at: 826 1045 3007 4142 4823 5014 826 Size: 219 1962 1135 681 191 1471 Fragments arranged by size: 1962 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5659 SmaI CCC'GGG Cuts at: 1872 2986 1872 Size: 1114 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3763 4664 4926 820 Size: 197 926 1820 901 262 1553 Fragments arranged by size: 1820 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3450 493 Size: 2957 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5659 SphI G_CATG'C Cuts at: 1132 3017 1132 Size: 1885 3774 SspI AAT'ATT Cuts at: 5 52 3583 5 Size: 47 3531 2081 Fragments arranged by size: 3531 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5659 StyI C'CwwG_G Cuts at: 513 1065 2355 2413 3098 513 Size: 552 1290 58 685 3074 Fragments arranged by size: 3074 1290 685 552 58 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2038 3686 3703 3856 4041 4486 325 Size: 1255 458 1648 17 153 185 445 1498 Fragments arranged by size: 1648 1498 1255 458 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3905 5617 96 Size: 274 80 33 51 1443 1928 1712 138 Fragments arranged by size: 1928 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5619 5651 5619 Size: 32 5627 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5659 XbaI T'CTAG_A Cuts at: 2995 2995 Size: 5659 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2122 2695 2122 Size: 573 5086 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5659 XmaI C'CCGG_G Cuts at: 1870 2984 1870 Size: 1114 4545 XmnI GAAnn'nnTTC Cuts at: 3788 3788 Size: 5659 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2668 3467 276 Size: 53 83 186 936 1134 799 2468 Fragments arranged by size: 2468 1134 936 799 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV FalI FseI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI