Restriction map for pFN21AB4203
 (Circular) (Six-base) MAPSORT of: pFN21AB4203.seq  Check: 4807  from: 1  to: 5659



pFN21AB4203  5659 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:46  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2746    2746 
   Size:       5659
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2670    3469     278 
   Size:         53      83     186     936    1134     799    2468

  Fragments arranged by size: 

                2468    1134     936     799     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2271    3002    1937 
   Size:        334     731    4594

  Fragments arranged by size: 

                4594     731     334
 
Acc65I G'GTAC_C

Cuts at:   2980    2980 
   Size:       5659
 
AclI AA'CG_TT

Cuts at:   1563    3786    4159    1563 
   Size:       2223     373    3063

  Fragments arranged by size: 

                3063    2223     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2771    3722    5106    5374    1632 
   Size:       1139     951    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1139     951     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5462
 
AflIII A'CryG_T

Cuts at:   1203    2322    4558    5535    1203 
   Size:       1119    2236     977    1327

  Fragments arranged by size: 

                2236    1327    1119     977
 
AgeI A'CCGG_T

Cuts at:   4490    4490 
   Size:       5659
 
AhdI GACnn_n'nnGTC

Cuts at:   4388    4388 
   Size:       5659
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5659
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5659
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2036    2068    2036 
   Size:         32    5627
 
AlwNI CAG_nnn'CTG

Cuts at:   2749    2833    3402    4974    2749 
   Size:         84     569    1572    3434

  Fragments arranged by size: 

                3434    1572     569      84
 
ApaI G_GGCC'C

Cuts at:   2446    2446 
   Size:       5659
 
ApaLI G'TGCA_C

Cuts at:   3716    4872    3716 
   Size:       1156    4503
 
ApoI r'AATT_y

Cuts at:   1493    2968    3194    3346    5455    1493 
   Size:       1475     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1475     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4213     160 
   Size:        872    3181    1606

  Fragments arranged by size: 

                3181    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5659
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2804    2984    1870 
   Size:         73     861     180    4545

  Fragments arranged by size: 

                4545     861     180      73
 
BamHI G'GATC_C

Cuts at:   2199    2989    3356    2199 
   Size:        790     367    4502

  Fragments arranged by size: 

                4502     790     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2177    2980    4435     618 
   Size:        325     217     627     390     803    1455    1842

  Fragments arranged by size: 

                1842    1455     803     627     390     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2343    2446    2978    1391 
   Size:        952     103     532    4072

  Fragments arranged by size: 

                4072     952     532     103
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2918    2958     928 
   Size:        971    1019      40    3629

  Fragments arranged by size: 

                3629    1019     971      40
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3850    3884    1903 
   Size:         34    1913      34    3678

  Fragments arranged by size: 

                3678    1913      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3551    4761    3551 
   Size:       1210    4449
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5659
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4270     136 
   Size:        107     122      71     631     605    2598    1525

  Fragments arranged by size: 

                2598    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5654    5654 
   Size:       5659
 
BlpI GC'TnA_GC

Cuts at:   3076    3076 
   Size:       5659
 
Bme1580I G_kGCm'C

Cuts at:   1790    2305    2446    3720    4876    1790 
   Size:        515     141    1274    1156    2573

  Fragments arranged by size: 

                2573    1274    1156     515     141
 
BmgBI CAC'GTC

Cuts at:   1318    2323    1318 
   Size:       1005    4654
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2135    3390    4348 
   Size:        416      53      83     399      24     718    1255     958

Cuts at:   4348     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1255     958     718     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5659
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2468    2692    2888    4319    1239 
   Size:       1229     224     196    1431    2579

  Fragments arranged by size: 

                2579    1431    1229     224     196
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3784    4649    4947    3784 
   Size:        865     298    4496

  Fragments arranged by size: 

                4496     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4322     882 
   Size:        617    2823    2219

  Fragments arranged by size: 

                2823    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3450     493 
   Size:        711    2246    2702

  Fragments arranged by size: 

                2702    2246     711
 
BsaBI GATnn'nnATC

Cuts at:   2702    5653    2702 
   Size:       2951    2708
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2667    3466    3848    4483 
   Size:         53      83     186     936    1134     799     382     635

Cuts at:   4483     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1134     936     799     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   2370    4092    4490    4764    4911    5322    2370 
   Size:       1722     398     274     147     411    2707

  Fragments arranged by size: 

                2707    1722     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2036    2066    2756    2783    2786 
   Size:         30     165      30     633      30     690      27       3

Cuts at:   2786    2813    1178 
   Size:         27    4024

  Fragments arranged by size: 

                4024     690     633     165      30      30      30      27
                  27       3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2780    1186 
   Size:       1594    4065
 
BseYI C'CCAG_C

Cuts at:   2778    2785    4862    5358    2778 
   Size:          7    2077     496    3079

  Fragments arranged by size: 

                3079    2077     496       7
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2441    1163 
   Size:        573     705    4381

  Fragments arranged by size: 

                4381     705     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2271    2794    3109    3870    4898    1540 
   Size:        275     186     270     523     315     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     523     315     275     270     186
            
 
BsiHKAI G_wGCw'C

Cuts at:   2343    2883    3720    3805    4876    2343 
   Size:        540     837      85    1071    3126

  Fragments arranged by size: 

                3126    1071     837     540      85
 
BsmI GAATG_Cn'

Cuts at:   3170    3263    3170 
   Size:         93    5566
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2313    2313 
   Size:       5659
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2305    2343    2446    2883    2978    3720    3805 
   Size:        399     515      38     103     437      95     742      85

Cuts at:   3805    4876    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     742     515     437     399     103      95
                  85      38
 
BspEI T'CCGG_A

Cuts at:   5322    5322 
   Size:       5659
 
BspHI T'CATG_A

Cuts at:   3548    5278    3548 
   Size:       1730    3929
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2746    3014     844 
   Size:       1902     268    3489

  Fragments arranged by size: 

                3489    1902     268
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2175    3546    1394 
   Size:        429     352    1371    3507

  Fragments arranged by size: 

                3507    1371     429     352
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2919    4154    4328      65 
   Size:       1183    1671    1235     174    1396

  Fragments arranged by size: 

                1671    1396    1235    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2591    4303    4490    1700 
   Size:        110     781    1712     187    2869

  Fragments arranged by size: 

                2869    1712     781     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5659
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5659
 
BssSI C'ACGA_G

Cuts at:   3719    4731    3719 
   Size:       1012    4647
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5659
 
BstBI TT'CG_AA

Cuts at:   4463    4463 
   Size:       5659
 
BstEII G'GTnAC_C

Cuts at:   1184    2110    1184 
   Size:        926    4733
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2584    2584 
   Size:       5659
 
BstYI r'GATC_y

Cuts at:   1972    2199    2328    2940    2989    3356    3741    3758    5210 
   Size:        227     129     612      49     367     385      17    1452

Cuts at:   5210    5296    5308    5654    1972 
   Size:         86      12     346    1977

  Fragments arranged by size: 

                1977    1452     612     385     367     346     227     129
                  86      49      17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2834    2834 
   Size:       5659
 
BtgI C'CryG_G

Cuts at:    513    1065    2131     513 
   Size:        552    1066    4041

  Fragments arranged by size: 

                4041    1066     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4920

  Fragments arranged by size: 

                4920     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2820    3175    3969    3989    2820 
   Size:        355     794      20    4490

  Fragments arranged by size: 

                4490     794     355      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5659
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5624
 
DraI TTT'AAA

Cuts at:   2963    2963 
   Size:       5659
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4666     809 
   Size:        507    3350    1802

  Fragments arranged by size: 

                3350    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2187    2574    2700 
   Size:         54    1425      50     275      18     357     387     126

Cuts at:   2700    3106    3995    5624       8 
   Size:        406     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     406     387     357     275     126
                  54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3106    1537 
   Size:        275    1294    4090

  Fragments arranged by size: 

                4090    1294     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3589    1353 
   Size:       2236    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4246    4620    4766     387 
   Size:       1064     138    2657     374     146    1280

  Fragments arranged by size: 

                2657    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2341    2341 
   Size:       5659
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2468    2692    2771    2888    3722    4319    5106 
   Size:        393     836     224      79     117     834     597     787

Cuts at:   5106    5374    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524     836     834     787     597     393     268     224
                 117      79
 
EcoO109I rG'GnC_Cy

Cuts at:   2443    2733    2443 
   Size:        290    5369
 
EcoRI G'AATT_C

Cuts at:   2968    3346    2968 
   Size:        378    5281
 
FspI TGC'GCA

Cuts at:   2264    4165    2264 
   Size:       1901    3758
 
FspAI rTGC'GCAy

Cuts at:   2264    2264 
   Size:       5659
 
HaeII r_GCGC'y

Cuts at:   1678    4806    1678 
   Size:       3128    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2756    2783    2788    2815    4300 
   Size:         32     135      32    1184      27       5      27    1485

Cuts at:   4300    4332    4374    4406    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1485    1184     135      42      32      32      32
                  32      27      27       5
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2272    3003    3249     669 
   Size:        988     281     334     731     246    3079

  Fragments arranged by size: 

                3079     988     731     334     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5659
 
HpaI GTT'AAC

Cuts at:   3249    3249 
   Size:       5659
 
KpnI G_GTAC'C

Cuts at:   2984    2984 
   Size:       5659
 
MfeI C'AATT_G

Cuts at:   3258    3258 
   Size:       5659
 
MluI A'CGCG_T

Cuts at:   5535    5535 
   Size:       5659
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2688    4773    4957    1305 
   Size:       1383    2085     184    2007

  Fragments arranged by size: 

                2085    2007    1383     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2576    2702      10 
   Size:         54    1425     343     744     126    2967

  Fragments arranged by size: 

                2967    1425     744     343     126      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2183    2189    2258    2484    2687    3617    3976 
   Size:        648    1017       6      69     226     203     930     359

Cuts at:   3976    4135     518 
   Size:        159    2042

  Fragments arranged by size: 

                2042    1017     930     648     359     226     203     159
                  69       6
 
MspA1I CmG'CkG

Cuts at:   1606    2749    3075    3752    4900    5145    1606 
   Size:       1143     326     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1143     677     326     245
 
NaeI GCC'GGC

Cuts at:   2593    2593 
   Size:       5659
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5107
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5659
 
NgoMIV G'CCGG_C

Cuts at:   2591    2591 
   Size:       5659
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5659
 
NotI GC'GGCC_GC

Cuts at:   3106    3106 
   Size:       5659
 
NspI r_CATG'y

Cuts at:   1132    1229    2263    3017    4562    1132 
   Size:         97    1034     754    1545    2229

  Fragments arranged by size: 

                2229    1545    1034     754      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5659
 
PciI A'CATG_T

Cuts at:   4558    4558 
   Size:       5659
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5659
 
PfoI T'CCnGG_A

Cuts at:   2054    2054 
   Size:       5659
 
PmeI GTTT'AAAC

Cuts at:   2963    2963 
   Size:       5659
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2036    2068    3728    3760    5241    5273    2036 
   Size:         32    1660      32    1481      32    2422

  Fragments arranged by size: 

                2422    1660    1481      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2733    2733 
   Size:       5659
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5659
 
PsiI TTA'TAA

Cuts at:   3229    3229 
   Size:       5659
 
PspOMI G'GGCC_C

Cuts at:   2442    2442 
   Size:       5659
 
PstI C_TGCA'G

Cuts at:    830    3011     830 
   Size:       2181    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5659
 
PvuII CAG'CTG

Cuts at:   2749    2749 
   Size:       5659
 
SacI G_AGCT'C

Cuts at:   2343    2343 
   Size:       5659
 
SalI G'TCGA_C

Cuts at:   1936    2270    3001    1936 
   Size:        334     731    4594

  Fragments arranged by size: 

                4594     731     334
 
SanDI GG'GwC_CC

Cuts at:   2733    2733 
   Size:       5659
 
SbfI CC_TGCA'GG

Cuts at:   3011    3011 
   Size:       5659
 
ScaI AGT'ACT

Cuts at:   3907    5619    3907 
   Size:       1712    3947
 
SfcI C'TryA_G

Cuts at:    826    1045    3007    4142    4823    5014     826 
   Size:        219    1962    1135     681     191    1471

  Fragments arranged by size: 

                1962    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5659
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5659
 
SmaI CCC'GGG

Cuts at:   1872    2986    1872 
   Size:       1114    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3763    4664    4926     820 
   Size:        197     926    1820     901     262    1553

  Fragments arranged by size: 

                1820    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3450     493 
   Size:       2957    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5659
 
SphI G_CATG'C

Cuts at:   1132    3017    1132 
   Size:       1885    3774
 
SspI AAT'ATT

Cuts at:      5      52    3583       5 
   Size:         47    3531    2081

  Fragments arranged by size: 

                3531    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5659
 
StyI C'CwwG_G

Cuts at:    513    1065    2355    2413    3098     513 
   Size:        552    1290      58     685    3074

  Fragments arranged by size: 

                3074    1290     685     552      58
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2038    3686    3703    3856    4041    4486     325 
   Size:       1255     458    1648      17     153     185     445    1498

  Fragments arranged by size: 

                1648    1498    1255     458     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3905    5617      96 
   Size:        274      80      33      51    1443    1928    1712     138

  Fragments arranged by size: 

                1928    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5619    5651    5619 
   Size:         32    5627
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5659
 
XbaI T'CTAG_A

Cuts at:   2995    2995 
   Size:       5659
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2122    2695    2122 
   Size:        573    5086
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5659
 
XmaI C'CCGG_G

Cuts at:   1870    2984    1870 
   Size:       1114    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3788    3788 
   Size:       5659
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2668    3467     276 
   Size:         53      83     186     936    1134     799    2468

  Fragments arranged by size: 

                2468    1134     936     799     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbsI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NcoI
     NdeI   NgoMIV     NheI     NotI     NspI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI     PstI
     PvuI    PvuII     SacI     SalI    SanDI     SbfI     ScaI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AscI    AvrII     BaeI     BbeI    BbvCI    BfrBI     BplI
   Bpu10I    BsiWI  BstZ17I   DraIII    EcoNI    EcoRV     FalI     FseI
     KasI     NarI     NruI     NsiI     PacI     PmlI    PspXI     PsrI
    RsrII    SacII     SapI    SexAI     SfiI     SfoI     SrfI     SwaI
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