Restriction map for
pFN21AB3461
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# NO CUT ENZYMES ( 193 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BclI BcnI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DdeI DpnII DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI Hin1I HincII HindII
HindIII HpaI HpaII HphI KasI
KpnI KspI MaeII MamI MboI
MfeI MflI MluI MluNI MroI
MscI MslI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NdeII NgoMIV NheI NlaIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI Sau3AI SbfI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SphI Sse8387I
SspBI StuI StyI SwaI Tsp45I
Tth111I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 595 GTAGAC GT^[AC][GT]AC
AspHI 275 GAGCAC G[AT]GC[AT]^C
BciVI 222 GGATAC GTATCC(6/5)
BmyI 275 GAGCAC G[AGT]GC[ACT]^C
BsiHKAI 275 GAGCAC G[AT]GC[AT]^C
BsiYI 34 CCAAATACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 34 CCAAATACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 481 GAGAC GTCTC(1/5)
Bsp1286I 275 GAGCAC G[AGT]GC[ACT]^C
BspHI 135 TCATGA T^CATGA
BstAPI 571 GCAAATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 485 CATCC GGATG(2/0)
Cac8I 360 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 14 GCGC GCG^C
Cfr13I 31 GGGCC G^G[ACGT]CC
EarI 587 GAAGAG CTCTTC(1/4)
EcoRV 301 GATATC GAT^ATC
FokI 485 CATCC GGATG(9/13)
HhaI 14 GCGC GCG^C
HinP1I 14 GCGC G^CGC
Ksp632I 587 GAAGAG CTCTTC(1/4)
MaeIII 641 GTAAC ^GT[ACGT]AC
MboII 587 GAAGA GAAGA(8/7)
MseI 344 TTAA T^TAA
PflMI 34 CCAAATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 227 CAGCTG CAG^CTG
RcaI 135 TCATGA T^CATGA
Sau96I 31 GGGCC G^G[ACGT]CC
ScaI 69 AGTACT AGT^ACT
SpeI 66 ACTAGT A^CTAGT
SspI 516 AATATT AAT^ATT
TaqI 9 TCGA T^CGA
TfiI 627 GAATC G^A[AT]TC
Tru9I 344 TTAA T^TAA
TspRI 631 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 9 TCGA T^CGA
Van91I 34 CCAAATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 11 enzymes) #
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AciI 86 CCGC CCGC(-3/-1)
488 CCGC
AcsI 139 GAATTT [GA]^AATT[CT]
573 AAATTC
ApoI 139 GAATTT [GA]^AATT[CT]
573 AAATTC
BbvI 283 GCAGC GCAGC(8/12)
163 GCTGC
BsrI 40 ACTGG ACTGG(1/-1)
57 CCAGT
Fnu4HI 163 GCTGC GC^[ACGT]GC
283 GCAGC
ItaI 163 GCTGC GC^[ACGT]GC
283 GCAGC
MspA1I 86 CCGCTG C[AC]G^C[GT]G
227 CAGCTG
PleI 82 GAGTC GAGTC(4/5)
316 GAGTC
SfcI 200 CTACAG C^T[GA][CT]AG
267 CTACAG
TseI 163 GCTGC G^C[AT]GC
283 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
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AfaI AluI BfaI BseRI BssKI
BstNI EcoRII HaeIII HinfI MaeI
MnlI MvaI MwoI NlaIII RsaI
ScrFI Tsp509I