Restriction map for pFN21AB3461
 (Circular) (Six-base) MAPSORT of: pFN21AB3461.seq  Check: 6518  from: 1  to: 5350



pFN21AB3461  5350 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:46  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3160     278 
   Size:         53      83     186     936    1624    2468

  Fragments arranged by size: 

                2468    1624     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2601    2693    1937 
   Size:        664      92    4594

  Fragments arranged by size: 

                4594     664      92
 
Acc65I G'GTAC_C

Cuts at:   2671    2671 
   Size:       5350
 
AclI AA'CG_TT

Cuts at:   1563    2649    3477    3850    1563 
   Size:       1086     828     373    3063

  Fragments arranged by size: 

                3063    1086     828     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2342    3413    4797    5065    1632 
   Size:        710    1071    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1071     710     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5153
 
AflIII A'CryG_T

Cuts at:   1203    4249    5226    1203 
   Size:       3046     977    1327

  Fragments arranged by size: 

                3046    1327     977
 
AgeI A'CCGG_T

Cuts at:   4181    4181 
   Size:       5350
 
AhdI GACnn_n'nnGTC

Cuts at:   4079    4079 
   Size:       5350
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5350
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5350
 
AlwNI CAG_nnn'CTG

Cuts at:   3093    4665    3093 
   Size:       1572    3778
 
ApaLI G'TGCA_C

Cuts at:   3407    4563    3407 
   Size:       1156    4194
 
ApoI r'AATT_y

Cuts at:   1493    2144    2578    2659    2885    3037    5146    1493 
   Size:        651     434      81     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     651     434     226     152      81
 
AseI AT'TA_AT

Cuts at:    160    1032    3904     160 
   Size:        872    2872    1606

  Fragments arranged by size: 

                2872    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5350
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2675    1870 
   Size:         73     732    4545

  Fragments arranged by size: 

                4545     732      73
 
BamHI G'GATC_C

Cuts at:   2680    3047    2680 
   Size:        367    4983
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2671    4126     618 
   Size:        325     217     627     884    1455    1842

  Fragments arranged by size: 

                1842    1455     884     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2669    1391 
   Size:       1278    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4379
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3541    3575    1903 
   Size:         34    1604      34    3678

  Fragments arranged by size: 

                3678    1604      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2221    3242    4452    2221 
   Size:       1021    1210    3119

  Fragments arranged by size: 

                3119    1210    1021
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5350
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3961     136 
   Size:        107     122      71     631     605    2289    1525

  Fragments arranged by size: 

                2289    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5345    5345 
   Size:       5350
 
BlpI GC'TnA_GC

Cuts at:   2767    2767 
   Size:       5350
 
Bme1580I G_kGCm'C

Cuts at:   1790    3411    4567    1790 
   Size:       1621    1156    2573

  Fragments arranged by size: 

                2573    1621    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5350
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3081    4039     442 
   Size:        416      53      83     399      24    1664     958    1753

  Fragments arranged by size: 

                1753    1664     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5350
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4010    1239 
   Size:       2771    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3475    4340    4638    3475 
   Size:        865     298    4187

  Fragments arranged by size: 

                4187     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4013     882 
   Size:        617    2514    2219

  Fragments arranged by size: 

                2514    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3141     493 
   Size:        711    1937    2702

  Fragments arranged by size: 

                2702    1937     711
 
BsaBI GATnn'nnATC

Cuts at:   5344    5344 
   Size:       5350
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3157    3539    4174     275 
   Size:         53      83     186     936    1624     382     635    1451

  Fragments arranged by size: 

                1624    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3783    4181    4455    4602    5013    3783 
   Size:        398     274     147     411    4120

  Fragments arranged by size: 

                4120     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5125

  Fragments arranged by size: 

                5125     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2292    2333    2610    1186 
   Size:       1106      41     277    3926

  Fragments arranged by size: 

                3926    1106     277      41
 
BseYI C'CCAG_C

Cuts at:   4553    5049    4553 
   Size:        496    4854
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4777
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2800    3561    4589    1540 
   Size:        275     186     799     761    1028    2301

  Fragments arranged by size: 

                2301    1028     799     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2284    3411    3496    4567    2284 
   Size:       1127      85    1071    3067

  Fragments arranged by size: 

                3067    1127    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2861    2954    2861 
   Size:         93    5257
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2284    2669    3411    3496    4567    1391 
   Size:        399     494     385     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     494     399     385      85
 
BspEI T'CCGG_A

Cuts at:   5013    5013 
   Size:       5350
 
BspHI T'CATG_A

Cuts at:   2140    3239    4969    2140 
   Size:       1099    1730    2521

  Fragments arranged by size: 

                2521    1730    1099
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2705     844 
   Size:       1861    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3237    1394 
   Size:        429    1414    3507

  Fragments arranged by size: 

                3507    1414     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3845    4019      65 
   Size:       1183    2597     174    1396

  Fragments arranged by size: 

                2597    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3994    4181    1700 
   Size:        110    2184     187    2869

  Fragments arranged by size: 

                2869    2184     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5350
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5350
 
BssSI C'ACGA_G

Cuts at:   3410    4422    3410 
   Size:       1012    4338
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2582     794 
   Size:       1788    3562
 
BstBI TT'CG_AA

Cuts at:   4154    4154 
   Size:       5350
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5350
 
BstYI r'GATC_y

Cuts at:   1972    2680    3047    3432    3449    4901    4987    4999    5345 
   Size:        708     367     385      17    1452      86      12     346

Cuts at:   5345    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     708     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3859

  Fragments arranged by size: 

                3859     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4611

  Fragments arranged by size: 

                4611     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2866    3660    3680    2866 
   Size:        794      20    4536

  Fragments arranged by size: 

                4536     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5350
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5315
 
DraI TTT'AAA

Cuts at:   2654    2654 
   Size:       5350
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4357     809 
   Size:        507    3041    1802

  Fragments arranged by size: 

                3041    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2797    3686    5315 
   Size:         54    1425      50     275      18     967     889    1629

Cuts at:   5315       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     967     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2797    1537 
   Size:        275     985    4090

  Fragments arranged by size: 

                4090     985     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2587    3280    1353 
   Size:       1234     693    3423

  Fragments arranged by size: 

                3423    1234     693
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3937    4311    4457     387 
   Size:       1064     138    2348     374     146    1280

  Fragments arranged by size: 

                2348    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2342    3413    4010    4797    5065    1239 
   Size:        393     710    1071     597     787     268    1524

  Fragments arranged by size: 

                1524    1071     787     710     597     393     268
 
EcoRI G'AATT_C

Cuts at:   2659    3037    2659 
   Size:        378    4972
 
EcoRV GAT'ATC

Cuts at:   2308    2308 
   Size:       5350
 
FspI TGC'GCA

Cuts at:   3856    3856 
   Size:       5350
 
HaeII r_GCGC'y

Cuts at:   1678    4497    1678 
   Size:       2819    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3991    4023    4065    4097    1373 
   Size:         32     135      32    2419      32      42      32    2626

  Fragments arranged by size: 

                2626    2419     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2694    2940     669 
   Size:        988     281     756     246    3079

  Fragments arranged by size: 

                3079     988     756     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5350
 
HpaI GTT'AAC

Cuts at:   2940    2940 
   Size:       5350
 
KpnI G_GTAC'C

Cuts at:   2675    2675 
   Size:       5350
 
MfeI C'AATT_G

Cuts at:   2949    2949 
   Size:       5350
 
MluI A'CGCG_T

Cuts at:   5226    5226 
   Size:       5350
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4464    4648    1305 
   Size:       3159     184    2007

  Fragments arranged by size: 

                3159    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3528

  Fragments arranged by size: 

                3528    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3308    3667    3826     518 
   Size:        648    2142     359     159    2042

  Fragments arranged by size: 

                2142    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2093    2234    2766    3443    4591    4836    1606 
   Size:        487     141     532     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     532     487     245     141
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3859

  Fragments arranged by size: 

                3859     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5350
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5350
 
NotI GC'GGCC_GC

Cuts at:   2797    2797 
   Size:       5350
 
NspI r_CATG'y

Cuts at:   1132    1229    2708    4253    1132 
   Size:         97    1479    1545    2229

  Fragments arranged by size: 

                2229    1545    1479      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5350
 
PciI A'CATG_T

Cuts at:   4249    4249 
   Size:       5350
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2045    1108 
   Size:        937    4413
 
PfoI T'CCnGG_A

Cuts at:   2439    2569    2439 
   Size:        130    5220
 
PmeI GTTT'AAAC

Cuts at:   2654    2654 
   Size:       5350
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3419    3451    4932    4964    3419 
   Size:         32    1481      32    3805

  Fragments arranged by size: 

                3805    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5350
 
PsiI TTA'TAA

Cuts at:   2920    2920 
   Size:       5350
 
PstI C_TGCA'G

Cuts at:    830    2702     830 
   Size:       1872    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5350
 
PvuII CAG'CTG

Cuts at:   2234    2234 
   Size:       5350
 
SalI G'TCGA_C

Cuts at:   1936    2692    1936 
   Size:        756    4594
 
SbfI CC_TGCA'GG

Cuts at:   2702    2702 
   Size:       5350
 
ScaI AGT'ACT

Cuts at:   2076    3598    5310    2076 
   Size:       1522    1712    2116

  Fragments arranged by size: 

                2116    1712    1522
 
SfcI C'TryA_G

Cuts at:    826    1045    2205    2272    2698    3833    4514    4705     826 
   Size:        219    1160      67     426    1135     681     191    1471

  Fragments arranged by size: 

                1471    1160    1135     681     426     219     191      67
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5350
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5350
 
SmaI CCC'GGG

Cuts at:   1872    2677    1872 
   Size:        805    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3454    4355    4617     820 
   Size:        197     926    1511     901     262    1553

  Fragments arranged by size: 

                1553    1511     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3141     493 
   Size:       2648    2702
 
SpeI A'CTAG_T

Cuts at:    152    2071     152 
   Size:       1919    3431
 
SphI G_CATG'C

Cuts at:   1132    2708    1132 
   Size:       1576    3774
 
SspI AAT'ATT

Cuts at:      5      52    2523    3274       5 
   Size:         47    2471     751    2081

  Fragments arranged by size: 

                2471    2081     751      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5350
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2789     513 
   Size:        552     939     785    3074

  Fragments arranged by size: 

                3074     939     785     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3377    3394    3547    3732    4177     325 
   Size:       1255    1797      17     153     185     445    1498

  Fragments arranged by size: 

                1797    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2074    2268    3596 
   Size:        274      80      33      51    1443      97     194    1328

Cuts at:   3596    5308      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1443    1328     274     194     138      97      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5310    5342    5310 
   Size:         32    5318
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5350
 
XbaI T'CTAG_A

Cuts at:   2686    2686 
   Size:       5350
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5350
 
XmaI C'CCGG_G

Cuts at:   1870    2675    1870 
   Size:        805    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3479    3479 
   Size:       5350
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3158     276 
   Size:         53      83     186     936    1624    2468

  Fragments arranged by size: 

                2468    1624     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI    EcoRI
    EcoRV     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NheI     NotI     NspI     PasI     PciI    PflMI     PfoI     PmeI
     PpiI    PshAI     PsiI     PstI     PvuI    PvuII     SalI     SbfI
     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I
   Bsu36I   DraIII  EcoICRI    EcoNI EcoO109I     FalI     FseI    FspAI
     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PmlI
    PpuMI   PspOMI    PspXI     PsrI    RsrII     SacI    SacII    SanDI
     SapI    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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