Restriction map for
pFN21AB3105
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# NO CUT ENZYMES ( 188 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaJI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstBI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I ClaI CpoI DdeI DraI
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRII EcoRV EcoT14I FauI
FbaI FokI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI KasI KpnI KspI
MaeII MaeIII MfeI MluI MluNI
MroI MscI MseI MslI MunI
MvaI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NlaIII NotI
NruI NspI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tru9I Tsp45I TspRI Tth111I Van91I
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 154 GAATTT [GA]^AATT[CT]
ApoI 154 GAATTT [GA]^AATT[CT]
BcnI 136 CCCGG CC^[GC]GG
BglII 340 AGATCT A^GATCT
BsaBI 304 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 458 GGTCTC GGTCTC(1/5)
BseRI 318 GAGGAG GAGGAG(10/8)
BspMI 377 GCAGGT ACCTGC(4/8)
BssKI 136 CCCGG ^CC[ACGT]GG
BstAPI 175 GCAGCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraII 379 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 209 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 401 GAAGAG CTCTTC(1/4)
EcoNI 449 CCTTCTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 379 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 1 ATGCAT ATGCA^T
HaeIII 271 GGCC GG^CC
HindIII 357 AAGCTT A^AGCTT
HphI 247 GGTGA GGTGA(8/7)
Ksp632I 401 GAAGAG CTCTTC(1/4)
MamI 304 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 176 CAGCTG C[AC]G^C[GT]G
NciI 136 CCCGG CC^[GC]GG
NlaIV 380 GGTCCC GG[ACGT]^[ACGT]CC
NsiI 1 ATGCAT ATGCA^T
PaeR7I 299 CTCGAG C^TCGAG
PleI 321 GAGTC GAGTC(4/5)
PpuMI 379 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 176 CAGCTG CAG^CTG
ScrFI 136 CCCGG CC^[ACGT]GG
SmlI 299 CTCGAG C^T[CT][GA]AG
TfiI 10 GATTC G^A[AT]TC
XbaI 388 TCTAGA T^CTAGA
XcmI 274 CCACCTCCCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 299 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AlwI 163 GATCC GGATC(4/5)
446 GATCC
AvaII 100 GGACC G^G[AT]CC
380 GGTCC
BsiYI 280 CCCTCTTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
449 CCTTCTCTAGG
BslI 280 CCCTCTTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
449 CCTTCTCTAGG
BsmAI 417 GTCTC GTCTC(1/5)
459 GTCTC
BsmFI 381 GTCCC GGGAC(10/14)
441 GTCCC
BstYI 162 AGATCC [GA]^GATC[CT]
340 AGATCT
HapII 137 CCGG C^CGG
372 CCGG
HinfI 10 GATTC G^A[ACGT]TC
321 GAGTC
HpaII 137 CCGG C^CGG
372 CCGG
MflI 162 AGATCC [GA]^GATC[CT]
340 AGATCT
MspI 137 CCGG C^CGG
372 CCGG
Tsp509I 74 AATT ^AATT
155 AATT
XhoII 162 AGATCC [GA]^GATC[CT]
340 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AluI AvaI BbvI BfaI BsoBI
Cfr13I DpnII Fnu4HI ItaI MaeI
MboI MboII MnlI MwoI NdeII
Sau3AI Sau96I SfaNI TaqI TseI
TthHB8I