Restriction map for
pFN21AB5306
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI Alw44I AlwI
AlwNI Aor51HI ApaLI AscI Asp700I
Asp718I AspI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcnI
BfrI BglI BglII BlnI BsaAI
BsaWI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspEI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI
BstEII BstPI BstUI BstYI Cfr10I
CpoI DraI DraIII DrdI EaeI
EagI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT22I FauI FseI
FspI HaeII HapII HpaI HpaII
KasI KpnI KspI MfeI MflI
MluI MluNI MroI MscI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AciI 161 CCGC CCGC(-3/-1)
AcyI 782 GACGCC G[GA]^CG[CT]C
AhdI 1600 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1910 CCTCAGG CC^T[ACGT]AGG
ApaI 1915 GGGCCC GGGCC^C
AseI 517 ATTAAT AT^TAAT
AsnI 517 ATTAAT AT^TAAT
AspEI 1600 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1403 CCCGAG C^[CT]CG[GA]G
BcgI 78 CGAGATAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1025 GTATCC GTATCC(6/5)
BclI 93 TGATCA T^GATCA
BfaI 976 CTAG C^TAG
BlpI 808 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 808 GCTAAGC GC^T[ACGT]AGC
BsaHI 782 GACGCC G[GA]^CG[CT]C
BsoBI 1403 CCCGAG C^[CT]CG[GA]G
BspDI 850 ATCGAT AT^CGAT
BsrDI 178 GCAATG GCAATG(2/0)
Bst1107I 1451 GTATAC GTA^TAC
BstZ17I 1451 GTATAC GTA^TAC
Bsu36I 1910 CCTCAGG CC^T[ACGT]AGG
CelII 808 GCTAAGC GC^T[ACGT]AGC
CfoI 738 GCGC GCG^C
ClaI 850 ATCGAT AT^CGAT
DsaI 930 CCGTGG C^C[GA][CT]GG
Eam1105I 1600 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1910 CCTCAGG CC^T[ACGT]AGG
EcoRI 1617 GAATTC G^AATTC
FbaI 93 TGATCA T^GATCA
HhaI 738 GCGC GCG^C
Hin1I 782 GACGCC G[GA]^CG[CT]C
HinP1I 738 GCGC G^CGC
HincII 712 GTCAAC GT[CT]^[GA]AC
HindII 712 GTCAAC GT[CT]^[GA]AC
MaeI 976 CTAG C^TAG
MslI 679 CACTTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 1847 GCATGC [GA]CATG^[CT]
PpuMI 9 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 517 ATTAAT AT^TAAT
PstI 14 CTGCAG CTGCA^G
SacI 1886 GAGCTC GAGCT^C
SmlI 1624 CTTGAG C^T[CT][GA]AG
SphI 1847 GCATGC GCATG^C
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccI 660 GTCTAC GT^[AC][GT]AC
1451 GTATAC
AcsI 1617 GAATTC [GA]^AATT[CT]
1771 GAATTT
ApoI 1617 GAATTC [GA]^AATT[CT]
1771 GAATTT
AspHI 583 GTGCTC G[AT]GC[AT]^C
1886 GAGCTC
BanI 34 GGCACC G^G[CT][GA]CC
934 GGCACC
BanII 1886 GAGCTC G[GA]GC[CT]^C
1915 GGGCCC
BsaBI 1500 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1744 GATAAGAATC
BsaI 328 GAGACC GGTCTC(1/5)
496 GGTCTC
BsiHKAI 583 GTGCTC G[AT]GC[AT]^C
1886 GAGCTC
BsmFI 9 GGGAC GGGAC(10/14)
1598 GGGAC
BsmI 915 GAATGC GAATGC(1/-1)
1859 GAATGC
BspHI 993 TCATGA T^CATGA
1226 TCATGA
BspMI 12 ACCTGC ACCTGC(4/8)
1361 ACCTGC
BstXI 419 CCAATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
678 CCACTTGGGTGG
DraII 9 GGGACCT [GA]G^G[ACGT]CC[CT]
1914 AGGGCCC
EarI 822 CTCTTC CTCTTC(1/4)
1927 GAAGAG
EcoO109I 9 GGGACCT [GA]G^G[ACGT]CC[CT]
1914 AGGGCCC
HindIII 336 AAGCTT A^AGCTT
1042 AAGCTT
Ksp632I 822 CTCTTC CTCTTC(1/4)
1927 GAAGAG
MamI 1500 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1744 GATAAGAATC
MspA1I 665 CAGCTG C[AC]G^C[GT]G
1118 CAGCTG
PvuII 665 CAGCTG CAG^CTG
1118 CAGCTG
RcaI 993 TCATGA T^CATGA
1226 TCATGA
SfcI 14 CTGCAG C^T[GA][CT]AG
662 CTACAG
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AfaI AluI AvaII BbsI BbvI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EcoRII EcoT14I
Fnu4HI FokI HaeIII HgaI HinfI
HphI ItaI MaeII MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I