(Circular) (Six-base) MAPSORT of: pFN21AB5306.seq Check: 9292 from: 1 to: 6643 pFN21AB5306 6643 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4453 278 Size: 53 83 186 936 2917 2468 Fragments arranged by size: 2917 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2666 3457 3986 1937 Size: 729 791 529 4594 Fragments arranged by size: 4594 791 729 529 Acc65I G'GTAC_C Cuts at: 3964 3964 Size: 6643 AclI AA'CG_TT Cuts at: 1563 4770 5143 1563 Size: 3207 373 3063 Fragments arranged by size: 3207 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3606 4706 6090 6358 1632 Size: 1974 1100 1384 268 1917 Fragments arranged by size: 1974 1917 1384 1100 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6446 AflIII A'CryG_T Cuts at: 1203 5542 6519 1203 Size: 4339 977 1327 Fragments arranged by size: 4339 1327 977 AgeI A'CCGG_T Cuts at: 5474 5474 Size: 6643 AhdI GACnn_n'nnGTC Cuts at: 3610 5372 3610 Size: 1762 4881 AleI CACnn'nnGTG Cuts at: 1166 2688 1166 Size: 1522 5121 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6643 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3543 3575 3543 Size: 32 6611 AlwNI CAG_nnn'CTG Cuts at: 4386 5958 4386 Size: 1572 5071 ApaI G_GGCC'C Cuts at: 3924 3924 Size: 6643 ApaLI G'TGCA_C Cuts at: 4700 5856 4700 Size: 1156 5487 ApoI r'AATT_y Cuts at: 1493 3622 3776 3952 4178 4330 6439 1493 Size: 2129 154 176 226 152 2109 1697 Fragments arranged by size: 2129 2109 1697 226 176 154 152 AseI AT'TA_AT Cuts at: 160 1032 2523 5197 160 Size: 872 1491 2674 1606 Fragments arranged by size: 2674 1606 1491 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6643 AvaI C'yCGr_G Cuts at: 1870 1943 3408 3968 1870 Size: 73 1465 560 4545 Fragments arranged by size: 4545 1465 560 73 BamHI G'GATC_C Cuts at: 3973 4340 3973 Size: 367 6276 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2039 2939 3964 5419 618 Size: 325 217 627 252 900 1025 1455 1842 Fragments arranged by size: 1842 1455 1025 900 627 325 252 217 BanII G_rGCy'C Cuts at: 1391 3895 3924 3962 1391 Size: 2504 29 38 4072 Fragments arranged by size: 4072 2504 38 29 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2355 2420 3549 928 Size: 971 456 65 1129 4022 Fragments arranged by size: 4022 1129 971 456 65 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2072 2106 4834 4868 1903 Size: 34 135 34 2728 34 3678 Fragments arranged by size: 3678 2728 135 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3041 4535 5745 3041 Size: 1494 1210 3939 Fragments arranged by size: 3939 1494 1210 BclI T'GATC_A Cuts at: 1548 2098 1548 Size: 550 6093 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5254 136 Size: 107 122 71 631 605 3582 1525 Fragments arranged by size: 3582 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6638 6638 Size: 6643 BlpI GC'TnA_GC Cuts at: 2814 4060 2814 Size: 1246 5397 Bme1580I G_kGCm'C Cuts at: 1790 2905 3924 4704 5860 1790 Size: 1115 1019 780 1156 2573 Fragments arranged by size: 2573 1156 1115 1019 780 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6643 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2945 4374 5332 Size: 416 53 83 399 24 1528 1429 958 Cuts at: 5332 442 Size: 1753 Fragments arranged by size: 1753 1528 1429 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6643 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2694 3168 3821 5303 1239 Size: 1455 474 653 1482 2579 Fragments arranged by size: 2579 1482 1455 653 474 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3650 4768 5633 5931 3650 Size: 1118 865 298 4362 Fragments arranged by size: 4362 1118 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2327 2507 5306 882 Size: 617 828 180 2799 2219 Fragments arranged by size: 2799 2219 828 617 180 BsaAI yAC'GTr Cuts at: 493 1204 4434 493 Size: 711 3230 2702 Fragments arranged by size: 3230 2702 711 BsaBI GATnn'nnATC Cuts at: 3509 3753 6637 3509 Size: 244 2884 3515 Fragments arranged by size: 3515 2884 244 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2788 4450 4832 5467 Size: 53 83 186 936 1255 1662 382 635 Cuts at: 5467 275 Size: 1451 Fragments arranged by size: 1662 1451 1255 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5076 5474 5748 5895 6306 5076 Size: 398 274 147 411 5413 Fragments arranged by size: 5413 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6418 Fragments arranged by size: 6418 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2369 3186 3885 1186 Size: 1183 817 699 3944 Fragments arranged by size: 3944 1183 817 699 BseYI C'CCAG_C Cuts at: 5846 6342 5846 Size: 496 6147 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3141 3387 3864 1163 Size: 573 1405 246 477 3942 Fragments arranged by size: 3942 1405 573 477 246 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4093 4854 5882 1540 Size: 275 186 2092 761 1028 2301 Fragments arranged by size: 2301 2092 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2592 3895 4704 4789 5860 2592 Size: 1303 809 85 1071 3375 Fragments arranged by size: 3375 1303 1071 809 85 BsmI GAATG_Cn' Cuts at: 2926 3870 4154 4247 2926 Size: 944 284 93 5322 Fragments arranged by size: 5322 944 284 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2592 2905 3895 3924 3962 4704 4789 Size: 399 802 313 990 29 38 742 85 Cuts at: 4789 5860 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 990 802 742 399 313 85 38 29 BspEI T'CCGG_A Cuts at: 6306 6306 Size: 6643 BspHI T'CATG_A Cuts at: 2998 3231 4532 6262 2998 Size: 233 1301 1730 3379 Fragments arranged by size: 3379 1730 1301 233 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2026 3375 3998 844 Size: 1182 1349 623 3489 Fragments arranged by size: 3489 1349 1182 623 BsrBI CCG'CTC Cuts at: 1394 1823 4530 1394 Size: 429 2707 3507 Fragments arranged by size: 3507 2707 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2190 5138 5312 65 Size: 1183 942 2948 174 1396 Fragments arranged by size: 2948 1396 1183 942 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5287 5474 1700 Size: 110 3477 187 2869 Fragments arranged by size: 3477 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6643 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6643 BssSI C'ACGA_G Cuts at: 4703 5715 4703 Size: 1012 5631 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6643 BstBI TT'CG_AA Cuts at: 5447 5447 Size: 6643 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6643 BstXI CCAn_nnnn'nTGG Cuts at: 2431 2690 2431 Size: 259 6384 BstYI r'GATC_y Cuts at: 1972 3973 4340 4725 4742 6194 6280 6292 6638 Size: 2001 367 385 17 1452 86 12 346 Cuts at: 6638 1972 Size: 1977 Fragments arranged by size: 2001 1977 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3458 3458 Size: 6643 Bsu36I CC'TnA_GG Cuts at: 3916 3916 Size: 6643 BtgI C'CryG_G Cuts at: 513 1065 2935 513 Size: 552 1870 4221 Fragments arranged by size: 4221 1870 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5904 Fragments arranged by size: 5904 683 56 BtsI GCAGTG_nn' Cuts at: 4159 4953 4973 4159 Size: 794 20 5829 Fragments arranged by size: 5829 794 20 ClaI AT'CG_AT Cuts at: 1554 2856 1554 Size: 1302 5341 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6608 DraI TTT'AAA Cuts at: 3947 3947 Size: 6643 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5650 809 Size: 507 4334 1802 Fragments arranged by size: 4334 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4090 4979 6608 Size: 54 1425 50 275 18 2260 889 1629 Cuts at: 6608 8 Size: 43 Fragments arranged by size: 2260 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4090 1537 Size: 275 2278 4090 Fragments arranged by size: 4090 2278 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2833 3927 4573 1353 Size: 1480 1094 646 3423 Fragments arranged by size: 3423 1480 1094 646 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5230 5604 5750 387 Size: 1064 138 3641 374 146 1280 Fragments arranged by size: 3641 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3893 3893 Size: 6643 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2694 3168 3606 3821 4706 5303 6090 Size: 393 1062 474 438 215 885 597 787 Cuts at: 6090 6358 1239 Size: 268 1524 Fragments arranged by size: 1524 1062 885 787 597 474 438 393 268 215 EcoO109I rG'GnC_Cy Cuts at: 2015 3920 2015 Size: 1905 4738 EcoRI G'AATT_C Cuts at: 3622 3952 4330 3622 Size: 330 378 5935 Fragments arranged by size: 5935 378 330 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2423 2423 Size: 6643 FspI TGC'GCA Cuts at: 5149 5149 Size: 6643 HaeII r_GCGC'y Cuts at: 1678 5790 1678 Size: 4112 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2345 2377 2648 2680 2845 Size: 32 135 32 773 32 271 32 165 Cuts at: 2845 2877 3596 3628 5284 5316 5358 5390 1373 Size: 32 719 32 1656 32 42 32 2626 Fragments arranged by size: 2626 1656 773 719 271 165 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2719 3987 4233 669 Size: 988 281 781 1268 246 3079 Fragments arranged by size: 3079 1268 988 781 281 246 HindIII A'AGCT_T Cuts at: 748 2341 3047 748 Size: 1593 706 4344 Fragments arranged by size: 4344 1593 706 HpaI GTT'AAC Cuts at: 4233 4233 Size: 6643 KpnI G_GTAC'C Cuts at: 3968 3968 Size: 6643 MfeI C'AATT_G Cuts at: 4242 4242 Size: 6643 MluI A'CGCG_T Cuts at: 6519 6519 Size: 6643 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2551 5757 5941 1305 Size: 1246 3206 184 2007 Fragments arranged by size: 3206 2007 1246 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4821 Fragments arranged by size: 4821 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2688 4601 4960 5119 518 Size: 648 1522 1913 359 159 2042 Fragments arranged by size: 2042 1913 1522 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2672 3125 4059 4736 5884 6129 1606 Size: 1066 453 934 677 1148 245 2120 Fragments arranged by size: 2120 1148 1066 934 677 453 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6091 NdeI CA'TA_TG Cuts at: 387 387 Size: 6643 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6643 NotI GC'GGCC_GC Cuts at: 4090 4090 Size: 6643 NspI r_CATG'y Cuts at: 1132 1229 3856 4001 5546 1132 Size: 97 2627 145 1545 2229 Fragments arranged by size: 2627 2229 1545 145 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6643 PciI A'CATG_T Cuts at: 5542 5542 Size: 6643 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6643 PfoI T'CCnGG_A Cuts at: 3561 3561 Size: 6643 PmeI GTTT'AAAC Cuts at: 3947 3947 Size: 6643 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4712 4744 6225 6257 4712 Size: 32 1481 32 5098 Fragments arranged by size: 5098 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2015 2015 Size: 6643 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6643 PsiI TTA'TAA Cuts at: 4213 4213 Size: 6643 PspOMI G'GGCC_C Cuts at: 3920 3920 Size: 6643 PstI C_TGCA'G Cuts at: 830 2023 3995 830 Size: 1193 1972 3478 Fragments arranged by size: 3478 1972 1193 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6643 PvuII CAG'CTG Cuts at: 2672 3125 2672 Size: 453 6190 SacI G_AGCT'C Cuts at: 3895 3895 Size: 6643 SalI G'TCGA_C Cuts at: 1936 3985 1936 Size: 2049 4594 SbfI CC_TGCA'GG Cuts at: 3995 3995 Size: 6643 ScaI AGT'ACT Cuts at: 4891 6603 4891 Size: 1712 4931 SfcI C'TryA_G Cuts at: 826 1045 2019 2667 3991 5126 5807 5998 826 Size: 219 974 648 1324 1135 681 191 1471 Fragments arranged by size: 1471 1324 1135 974 681 648 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6643 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6643 SmaI CCC'GGG Cuts at: 1872 3970 1872 Size: 2098 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3629 4747 5648 5910 820 Size: 197 926 1686 1118 901 262 1553 Fragments arranged by size: 1686 1553 1118 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4434 493 Size: 3941 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6643 SphI G_CATG'C Cuts at: 1132 3856 4001 1132 Size: 2724 145 3774 Fragments arranged by size: 3774 2724 145 SspI AAT'ATT Cuts at: 5 52 4567 5 Size: 47 4515 2081 Fragments arranged by size: 4515 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6643 StyI C'CwwG_G Cuts at: 513 1065 2010 2307 2407 3205 4082 513 Size: 552 945 297 100 798 877 3074 Fragments arranged by size: 3074 945 877 798 552 297 100 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2048 2678 2696 2852 4670 4687 4840 Size: 1255 468 630 18 156 1818 17 153 Cuts at: 4840 5025 5470 325 Size: 185 445 1498 Fragments arranged by size: 1818 1498 1255 630 468 445 185 156 153 18 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2509 3576 4889 Size: 274 80 33 51 1443 532 1067 1313 Cuts at: 4889 6601 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1313 1067 532 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6603 6635 6603 Size: 32 6611 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6643 XbaI T'CTAG_A Cuts at: 3979 3979 Size: 6643 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6643 XmaI C'CCGG_G Cuts at: 1870 3968 1870 Size: 2098 4545 XmnI GAAnn'nnTTC Cuts at: 4772 4772 Size: 6643 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4451 276 Size: 53 83 186 936 2917 2468 Fragments arranged by size: 2917 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI