Restriction map for
pFN21AB5306
(Linear) (Six-base) MAPSORT of: KIBB5306.seq Check: 7268 from: 1 to: 1938
KIBB5306.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 0 661 1452 1938
Size: 661 791 486
Fragments arranged by size:
791 661 486
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1601 1938
Size: 1601 337
AhdI GACnn_n'nnGTC
Cuts at: 0 1605 1938
Size: 1605 333
AleI CACnn'nnGTG
Cuts at: 0 683 1938
Size: 683 1255
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1538 1570 1938
Size: 1538 32 368
Fragments arranged by size:
1538 368 32
ApaI G_GGCC'C
Cuts at: 0 1919 1938
Size: 1919 19
ApoI r'AATT_y
Cuts at: 0 1617 1771 1938
Size: 1617 154 167
Fragments arranged by size:
1617 167 154
AseI AT'TA_AT
Cuts at: 0 518 1938
Size: 518 1420
AvaI C'yCGr_G
Cuts at: 0 1403 1938
Size: 1403 535
BanI G'GyrC_C
Cuts at: 0 34 934 1938
Size: 34 900 1004
Fragments arranged by size:
1004 900 34
BanII G_rGCy'C
Cuts at: 0 1890 1919 1938
Size: 1890 29 19
Fragments arranged by size:
1890 29 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 350 415 1544 1938
Size: 350 65 1129 394
Fragments arranged by size:
1129 394 350 65
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 67 101 1938
Size: 67 34 1837
Fragments arranged by size:
1837 67 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1036 1938
Size: 1036 902
BclI T'GATC_A
Cuts at: 0 93 1938
Size: 93 1845
BlpI GC'TnA_GC
Cuts at: 0 809 1938
Size: 809 1129
Bme1580I G_kGCm'C
Cuts at: 0 900 1919 1938
Size: 900 1019 19
Fragments arranged by size:
1019 900 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 940 1938
Size: 940 998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 689 1163 1816 1938
Size: 689 474 653 122
Fragments arranged by size:
689 653 474 122
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1645 1938
Size: 1645 293
BsaI GGTCTCn'nnnn_
Cuts at: 0 322 502 1938
Size: 322 180 1436
Fragments arranged by size:
1436 322 180
BsaBI GATnn'nnATC
Cuts at: 0 1504 1748 1938
Size: 1504 244 190
Fragments arranged by size:
1504 244 190
BsaHI Gr'CG_yC
Cuts at: 0 783 1938
Size: 783 1155
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 364 1181 1880 1938
Size: 364 817 699 58
Fragments arranged by size:
817 699 364 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1136 1382 1859 1938
Size: 1136 246 477 79
Fragments arranged by size:
1136 477 246 79
BsiHKAI G_wGCw'C
Cuts at: 0 587 1890 1938
Size: 587 1303 48
Fragments arranged by size:
1303 587 48
BsmI GAATG_Cn'
Cuts at: 0 921 1865 1938
Size: 921 944 73
Fragments arranged by size:
944 921 73
Bsp1286I G_dGCh'C
Cuts at: 0 587 900 1890 1919 1938
Size: 587 313 990 29 19
Fragments arranged by size:
990 587 313 29 19
BspHI T'CATG_A
Cuts at: 0 993 1226 1938
Size: 993 233 712
Fragments arranged by size:
993 712 233
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 21 1370 1938
Size: 21 1349 568
Fragments arranged by size:
1349 568 21
BsrDI GCAATG_nn'
Cuts at: 0 185 1938
Size: 185 1753
BstXI CCAn_nnnn'nTGG
Cuts at: 0 426 685 1938
Size: 426 259 1253
Fragments arranged by size:
1253 426 259
BstZ17I GTA'TAC
Cuts at: 0 1453 1938
Size: 1453 485
Bsu36I CC'TnA_GG
Cuts at: 0 1911 1938
Size: 1911 27
BtgI C'CryG_G
Cuts at: 0 930 1938
Size: 930 1008
ClaI AT'CG_AT
Cuts at: 0 851 1938
Size: 851 1087
EarI CTCTTCn'nnn_
Cuts at: 0 828 1922 1938
Size: 828 1094 16
Fragments arranged by size:
1094 828 16
EcoICRI GAG'CTC
Cuts at: 0 1888 1938
Size: 1888 50
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 689 1163 1601 1816 1938
Size: 689 474 438 215 122
Fragments arranged by size:
689 474 438 215 122
EcoO109I rG'GnC_Cy
Cuts at: 0 10 1915 1938
Size: 10 1905 23
Fragments arranged by size:
1905 23 10
EcoRI G'AATT_C
Cuts at: 0 1617 1938
Size: 1617 321
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 418 1938
Size: 418 1520
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 340 372 643 675 840 872 1591 1623
Size: 340 32 271 32 165 32 719 32
Cuts at: 1623 1938
Size: 315
Fragments arranged by size:
719 340 315 271 165 32 32 32
32
HincII GTy'rAC
Cuts at: 0 714 1938
Size: 714 1224
HindIII A'AGCT_T
Cuts at: 0 336 1042 1938
Size: 336 706 896
Fragments arranged by size:
896 706 336
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 546 1938
Size: 546 1392
MslI CAynn'nnrTG
Cuts at: 0 683 1938
Size: 683 1255
MspA1I CmG'CkG
Cuts at: 0 667 1120 1938
Size: 667 453 818
Fragments arranged by size:
818 667 453
NspI r_CATG'y
Cuts at: 0 1851 1938
Size: 1851 87
PfoI T'CCnGG_A
Cuts at: 0 1556 1938
Size: 1556 382
PpuMI rG'GwC_Cy
Cuts at: 0 10 1938
Size: 10 1928
PspOMI G'GGCC_C
Cuts at: 0 1915 1938
Size: 1915 23
PstI C_TGCA'G
Cuts at: 0 18 1938
Size: 18 1920
PvuII CAG'CTG
Cuts at: 0 667 1120 1938
Size: 667 453 818
Fragments arranged by size:
818 667 453
SacI G_AGCT'C
Cuts at: 0 1890 1938
Size: 1890 48
SfcI C'TryA_G
Cuts at: 0 14 662 1938
Size: 14 648 1276
Fragments arranged by size:
1276 648 14
SmlI C'TyrA_G
Cuts at: 0 1624 1938
Size: 1624 314
SphI G_CATG'C
Cuts at: 0 1851 1938
Size: 1851 87
StyI C'CwwG_G
Cuts at: 0 5 302 402 1200 1938
Size: 5 297 100 798 738
Fragments arranged by size:
798 738 297 100 5
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 43 673 691 847 1938
Size: 43 630 18 156 1091
Fragments arranged by size:
1091 630 156 43 18
TatI w'GTAC_w
Cuts at: 0 504 1571 1938
Size: 504 1067 367
Fragments arranged by size:
1067 504 367
Enzymes that do cut:
AccI AcuI AhdI AleI AloI ApaI ApoI AseI
AvaI BanI BanII BbsI BcgI BciVI BclI BlpI
Bme1580I BmrI BpmI BpuEI BsaI BsaBI BsaHI BseRI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BstXI
BstZ17I Bsu36I BtgI ClaI EarI EcoICRI Eco57MI EcoO109I
EcoRI FalI Hin4I HincII HindIII MmeI MslI MspA1I
NspI PfoI PpuMI PspOMI PstI PvuII SacI SfcI
SmlI SphI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AlfI AlwNI ApaLI AscI AsiSI AvrII BaeI BamHI
BbeI BbvCI BfrBI BglI BglII BmgBI BmtI BplI
Bpu10I BsaAI BsaWI BsaXI BseYI BsiEI BsiWI BsmBI
BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EciI EcoNI EcoRV FseI FspI FspAI
HaeII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StuI SwaI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI