(Circular) (Six-base) MAPSORT of: pFN21AB5019.seq Check: 5571 from: 1 to: 6220 pFN21AB5019 6220 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2330 2330 Size: 6220 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4030 278 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3563 1937 Size: 1626 4594 Acc65I G'GTAC_C Cuts at: 3541 3541 Size: 6220 AclI AA'CG_TT Cuts at: 1563 4347 4720 1563 Size: 2784 373 3063 Fragments arranged by size: 3063 2784 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2333 2394 3107 3119 4283 5667 5935 1632 Size: 701 61 713 12 1164 1384 268 1917 Fragments arranged by size: 1917 1384 1164 713 701 268 61 12 AfeI AGC'GCT Cuts at: 2025 2025 Size: 6220 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6023 AflIII A'CryG_T Cuts at: 1203 5119 6096 1203 Size: 3916 977 1327 Fragments arranged by size: 3916 1327 977 AgeI A'CCGG_T Cuts at: 5051 5051 Size: 6220 AhdI GACnn_n'nnGTC Cuts at: 4949 4949 Size: 6220 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6220 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6220 AlwNI CAG_nnn'CTG Cuts at: 3963 5535 3963 Size: 1572 4648 ApaLI G'TGCA_C Cuts at: 4277 5433 4277 Size: 1156 5064 ApoI r'AATT_y Cuts at: 1493 2435 2444 2870 3157 3203 3529 3755 3907 Size: 942 9 426 287 46 326 226 152 Cuts at: 3907 6016 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 942 426 326 287 226 152 46 9 AseI AT'TA_AT Cuts at: 160 1032 4774 160 Size: 872 3742 1606 Fragments arranged by size: 3742 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 AvaI C'yCGr_G Cuts at: 1870 1943 2032 3545 1870 Size: 73 89 1513 4545 Fragments arranged by size: 4545 1513 89 73 AvrII C'CTAG_G Cuts at: 2357 2357 Size: 6220 BamHI G'GATC_C Cuts at: 3550 3917 3550 Size: 367 5853 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3541 4996 618 Size: 325 217 627 1754 1455 1842 Fragments arranged by size: 1842 1754 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2135 3246 3539 1391 Size: 744 1111 293 4072 Fragments arranged by size: 4072 1111 744 293 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2757 928 Size: 971 858 4391 Fragments arranged by size: 4391 971 858 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4411 4445 1903 Size: 34 50 34 2390 34 3678 Fragments arranged by size: 3678 2390 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4112 5322 4112 Size: 1210 5010 BclI T'GATC_A Cuts at: 1548 1548 Size: 6220 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4831 136 Size: 107 122 71 631 605 3159 1525 Fragments arranged by size: 3159 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6215 6215 Size: 6220 BlpI GC'TnA_GC Cuts at: 3272 3637 3272 Size: 365 5855 Bme1580I G_kGCm'C Cuts at: 1790 2889 4281 5437 1790 Size: 1099 1392 1156 2573 Fragments arranged by size: 2573 1392 1156 1099 BmgBI CAC'GTC Cuts at: 1318 2083 1318 Size: 765 5455 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3951 4909 442 Size: 416 53 83 399 24 2534 958 1753 Fragments arranged by size: 2534 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2913 1056 Size: 1857 4363 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2524 3046 2524 Size: 522 5698 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2860 2985 3071 3260 3357 4880 1239 Size: 1621 125 86 189 97 1523 2579 Fragments arranged by size: 2579 1621 1523 189 125 97 86 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2557 4345 5210 5508 2557 Size: 1788 865 298 3269 Fragments arranged by size: 3269 1788 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4883 882 Size: 617 3384 2219 Fragments arranged by size: 3384 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4011 493 Size: 711 2807 2702 Fragments arranged by size: 2807 2702 711 BsaBI GATnn'nnATC Cuts at: 3081 6214 3081 Size: 3133 3087 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4027 4409 5044 275 Size: 53 83 186 936 2494 382 635 1451 Fragments arranged by size: 2494 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4653 5051 5325 5472 5883 4653 Size: 398 274 147 411 4990 Fragments arranged by size: 4990 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3233 3263 1178 Size: 30 165 30 1830 30 4135 Fragments arranged by size: 4135 1830 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3038 1186 Size: 1852 4368 BseYI C'CCAG_C Cuts at: 2882 5423 5919 2882 Size: 2541 496 3183 Fragments arranged by size: 3183 2541 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5647 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3670 4431 5459 1540 Size: 275 186 1669 761 1028 2301 Fragments arranged by size: 2301 1669 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2960 4281 4366 5437 2960 Size: 1321 85 1071 3743 Fragments arranged by size: 3743 1321 1071 85 BsmI GAATG_Cn' Cuts at: 3731 3824 3731 Size: 93 6127 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2135 2889 2960 3246 3539 4281 4366 Size: 399 345 754 71 286 293 742 85 Cuts at: 4366 5437 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 754 742 399 345 293 286 85 71 BspEI T'CCGG_A Cuts at: 5883 5883 Size: 6220 BspHI T'CATG_A Cuts at: 4109 5839 4109 Size: 1730 4490 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2330 3575 844 Size: 1486 1245 3489 Fragments arranged by size: 3489 1486 1245 BsrBI CCG'CTC Cuts at: 1394 1823 4107 1394 Size: 429 2284 3507 Fragments arranged by size: 3507 2284 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3130 4715 4889 65 Size: 1183 1882 1585 174 1396 Fragments arranged by size: 1882 1585 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4864 5051 1700 Size: 110 3054 187 2869 Fragments arranged by size: 3054 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6220 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6220 BssSI C'ACGA_G Cuts at: 4280 5292 4280 Size: 1012 5208 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6220 BstBI TT'CG_AA Cuts at: 5024 5024 Size: 6220 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6220 BstYI r'GATC_y Cuts at: 1972 3514 3550 3917 4302 4319 5771 5857 5869 Size: 1542 36 367 385 17 1452 86 12 Cuts at: 5869 6215 1972 Size: 346 1977 Fragments arranged by size: 1977 1542 1452 385 367 346 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2055 513 Size: 552 990 4678 Fragments arranged by size: 4678 990 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5481 Fragments arranged by size: 5481 683 56 BtsI GCAGTG_nn' Cuts at: 2179 2259 2958 3736 4530 4550 2179 Size: 80 699 778 794 20 3849 Fragments arranged by size: 3849 794 778 699 80 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6220 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6185 DraI TTT'AAA Cuts at: 3524 3524 Size: 6220 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5227 809 Size: 507 3911 1802 Fragments arranged by size: 3911 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3412 3667 4556 Size: 54 1425 50 275 18 1582 255 889 Cuts at: 4556 6185 8 Size: 1629 43 Fragments arranged by size: 1629 1582 1425 889 275 255 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3667 1537 Size: 275 1855 4090 Fragments arranged by size: 4090 1855 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3083 4150 1353 Size: 1730 1067 3423 Fragments arranged by size: 3423 1730 1067 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2003 4807 5181 5327 387 Size: 1064 138 414 2804 374 146 1280 Fragments arranged by size: 2804 1280 1064 414 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2333 2394 2860 2985 3071 3107 3119 Size: 393 701 61 466 125 86 36 12 Cuts at: 3119 3260 3357 4283 4880 5667 5935 1239 Size: 141 97 926 597 787 268 1524 Fragments arranged by size: 1524 926 787 701 597 466 393 268 141 125 97 86 61 36 12 EcoRI G'AATT_C Cuts at: 3529 3907 3529 Size: 378 5842 EcoRV GAT'ATC Cuts at: 2303 2303 Size: 6220 FspI TGC'GCA Cuts at: 4726 4726 Size: 6220 HaeII r_GCGC'y Cuts at: 1678 2027 2045 5367 1678 Size: 349 18 3322 2531 Fragments arranged by size: 3322 2531 349 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2117 2149 2595 2627 4861 Size: 32 135 32 545 32 446 32 2234 Cuts at: 4861 4893 4935 4967 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2234 545 446 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3564 3810 669 Size: 988 281 1626 246 3079 Fragments arranged by size: 3079 1626 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6220 HpaI GTT'AAC Cuts at: 3810 3810 Size: 6220 KpnI G_GTAC'C Cuts at: 3545 3545 Size: 6220 MfeI C'AATT_G Cuts at: 3819 3819 Size: 6220 MluI A'CGCG_T Cuts at: 6096 6096 Size: 6220 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2103 2148 2330 2856 5334 5518 1305 Size: 798 45 182 526 2478 184 2007 Fragments arranged by size: 2478 2007 798 526 184 182 45 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4398 Fragments arranged by size: 4398 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2565 4178 4537 4696 518 Size: 648 1399 1613 359 159 2042 Fragments arranged by size: 2042 1613 1399 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2057 2072 3636 4313 5461 5706 1606 Size: 451 15 1564 677 1148 245 2120 Fragments arranged by size: 2120 1564 1148 677 451 245 15 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5668 NdeI CA'TA_TG Cuts at: 387 3337 387 Size: 2950 3270 NheI G'CTAG_C Cuts at: 1052 2909 1052 Size: 1857 4363 NotI GC'GGCC_GC Cuts at: 3667 3667 Size: 6220 NspI r_CATG'y Cuts at: 1132 1229 3578 5123 1132 Size: 97 2349 1545 2229 Fragments arranged by size: 2349 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6220 PciI A'CATG_T Cuts at: 5119 5119 Size: 6220 PflMI CCAn_nnn'nTGG Cuts at: 1108 2838 1108 Size: 1730 4490 PfoI T'CCnGG_A Cuts at: 2712 2874 2712 Size: 162 6058 PmeI GTTT'AAAC Cuts at: 3524 3524 Size: 6220 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4289 4321 5802 5834 4289 Size: 32 1481 32 4675 Fragments arranged by size: 4675 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6220 PsiI TTA'TAA Cuts at: 3790 3790 Size: 6220 PstI C_TGCA'G Cuts at: 830 2215 3066 3572 830 Size: 1385 851 506 3478 Fragments arranged by size: 3478 1385 851 506 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6220 SacII CC_GC'GG Cuts at: 2058 2058 Size: 6220 SalI G'TCGA_C Cuts at: 1936 3562 1936 Size: 1626 4594 SbfI CC_TGCA'GG Cuts at: 3066 3572 3066 Size: 506 5714 ScaI AGT'ACT Cuts at: 4468 6180 4468 Size: 1712 4508 SfcI C'TryA_G Cuts at: 826 1045 2211 2469 2904 3062 3568 4703 5384 Size: 219 1166 258 435 158 506 1135 681 Cuts at: 5384 5575 826 Size: 191 1471 Fragments arranged by size: 1471 1166 1135 681 506 435 258 219 191 158 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6220 SmaI CCC'GGG Cuts at: 1872 2034 3547 1872 Size: 162 1513 4545 Fragments arranged by size: 4545 1513 162 SmlI C'TyrA_G Cuts at: 820 1017 1943 2536 4324 5225 5487 820 Size: 197 926 593 1788 901 262 1553 Fragments arranged by size: 1788 1553 926 901 593 262 197 SnaBI TAC'GTA Cuts at: 493 4011 493 Size: 3518 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6220 SphI G_CATG'C Cuts at: 1132 3578 1132 Size: 2446 3774 SspI AAT'ATT Cuts at: 5 52 4144 5 Size: 47 4092 2081 Fragments arranged by size: 4092 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6220 StyI C'CwwG_G Cuts at: 513 1065 2357 3215 3659 513 Size: 552 1292 858 444 3074 Fragments arranged by size: 3074 1292 858 552 444 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4247 4264 4417 4602 5047 325 Size: 1255 2667 17 153 185 445 1498 Fragments arranged by size: 2667 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4466 6178 96 Size: 274 80 33 51 1443 2489 1712 138 Fragments arranged by size: 2489 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6180 6212 6180 Size: 32 6188 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6220 XbaI T'CTAG_A Cuts at: 3556 3556 Size: 6220 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6220 XmaI C'CCGG_G Cuts at: 1870 2032 3545 1870 Size: 162 1513 4545 Fragments arranged by size: 4545 1513 162 XmnI GAAnn'nnTTC Cuts at: 4349 4349 Size: 6220 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4028 276 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI