Restriction map for pFN21AB5019
 (Circular) (Six-base) MAPSORT of: pFN21AB5019.seq  Check: 5571  from: 1  to: 6220



pFN21AB5019  6220 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:34  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2330    2330 
   Size:       6220
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4030     278 
   Size:         53      83     186     936    2494    2468

  Fragments arranged by size: 

                2494    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3563    1937 
   Size:       1626    4594
 
Acc65I G'GTAC_C

Cuts at:   3541    3541 
   Size:       6220
 
AclI AA'CG_TT

Cuts at:   1563    4347    4720    1563 
   Size:       2784     373    3063

  Fragments arranged by size: 

                3063    2784     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2333    2394    3107    3119    4283    5667    5935    1632 
   Size:        701      61     713      12    1164    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1164     713     701     268      61      12
            
 
AfeI AGC'GCT

Cuts at:   2025    2025 
   Size:       6220
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6023
 
AflIII A'CryG_T

Cuts at:   1203    5119    6096    1203 
   Size:       3916     977    1327

  Fragments arranged by size: 

                3916    1327     977
 
AgeI A'CCGG_T

Cuts at:   5051    5051 
   Size:       6220
 
AhdI GACnn_n'nnGTC

Cuts at:   4949    4949 
   Size:       6220
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       6220
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6220
 
AlwNI CAG_nnn'CTG

Cuts at:   3963    5535    3963 
   Size:       1572    4648
 
ApaLI G'TGCA_C

Cuts at:   4277    5433    4277 
   Size:       1156    5064
 
ApoI r'AATT_y

Cuts at:   1493    2435    2444    2870    3157    3203    3529    3755    3907 
   Size:        942       9     426     287      46     326     226     152

Cuts at:   3907    6016    1493 
   Size:       2109    1697

  Fragments arranged by size: 

                2109    1697     942     426     326     287     226     152
                  46       9
 
AseI AT'TA_AT

Cuts at:    160    1032    4774     160 
   Size:        872    3742    1606

  Fragments arranged by size: 

                3742    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6220
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2032    3545    1870 
   Size:         73      89    1513    4545

  Fragments arranged by size: 

                4545    1513      89      73
 
AvrII C'CTAG_G

Cuts at:   2357    2357 
   Size:       6220
 
BamHI G'GATC_C

Cuts at:   3550    3917    3550 
   Size:        367    5853
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3541    4996     618 
   Size:        325     217     627    1754    1455    1842

  Fragments arranged by size: 

                1842    1754    1455     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2135    3246    3539    1391 
   Size:        744    1111     293    4072

  Fragments arranged by size: 

                4072    1111     744     293
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2757     928 
   Size:        971     858    4391

  Fragments arranged by size: 

                4391     971     858
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    4411    4445    1903 
   Size:         34      50      34    2390      34    3678

  Fragments arranged by size: 

                3678    2390      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4112    5322    4112 
   Size:       1210    5010
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6220
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4831     136 
   Size:        107     122      71     631     605    3159    1525

  Fragments arranged by size: 

                3159    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   6215    6215 
   Size:       6220
 
BlpI GC'TnA_GC

Cuts at:   3272    3637    3272 
   Size:        365    5855
 
Bme1580I G_kGCm'C

Cuts at:   1790    2889    4281    5437    1790 
   Size:       1099    1392    1156    2573

  Fragments arranged by size: 

                2573    1392    1156    1099
 
BmgBI CAC'GTC

Cuts at:   1318    2083    1318 
   Size:        765    5455
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3951    4909     442 
   Size:        416      53      83     399      24    2534     958    1753

  Fragments arranged by size: 

                2534    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    2913    1056 
   Size:       1857    4363
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2524    3046    2524 
   Size:        522    5698
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2860    2985    3071    3260    3357    4880    1239 
   Size:       1621     125      86     189      97    1523    2579

  Fragments arranged by size: 

                2579    1621    1523     189     125      97      86
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2557    4345    5210    5508    2557 
   Size:       1788     865     298    3269

  Fragments arranged by size: 

                3269    1788     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4883     882 
   Size:        617    3384    2219

  Fragments arranged by size: 

                3384    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    4011     493 
   Size:        711    2807    2702

  Fragments arranged by size: 

                2807    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   3081    6214    3081 
   Size:       3133    3087
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4027    4409    5044     275 
   Size:         53      83     186     936    2494     382     635    1451

  Fragments arranged by size: 

                2494    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4653    5051    5325    5472    5883    4653 
   Size:        398     274     147     411    4990

  Fragments arranged by size: 

                4990     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    3233    3263    1178 
   Size:         30     165      30    1830      30    4135

  Fragments arranged by size: 

                4135    1830     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    3038    1186 
   Size:       1852    4368
 
BseYI C'CCAG_C

Cuts at:   2882    5423    5919    2882 
   Size:       2541     496    3183

  Fragments arranged by size: 

                3183    2541     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5647
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3670    4431    5459    1540 
   Size:        275     186    1669     761    1028    2301

  Fragments arranged by size: 

                2301    1669    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2960    4281    4366    5437    2960 
   Size:       1321      85    1071    3743

  Fragments arranged by size: 

                3743    1321    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3731    3824    3731 
   Size:         93    6127
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2135    2889    2960    3246    3539    4281    4366 
   Size:        399     345     754      71     286     293     742      85

Cuts at:   4366    5437    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     754     742     399     345     293     286
                  85      71
 
BspEI T'CCGG_A

Cuts at:   5883    5883 
   Size:       6220
 
BspHI T'CATG_A

Cuts at:   4109    5839    4109 
   Size:       1730    4490
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2330    3575     844 
   Size:       1486    1245    3489

  Fragments arranged by size: 

                3489    1486    1245
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4107    1394 
   Size:        429    2284    3507

  Fragments arranged by size: 

                3507    2284     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3130    4715    4889      65 
   Size:       1183    1882    1585     174    1396

  Fragments arranged by size: 

                1882    1585    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4864    5051    1700 
   Size:        110    3054     187    2869

  Fragments arranged by size: 

                3054    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6220
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6220
 
BssSI C'ACGA_G

Cuts at:   4280    5292    4280 
   Size:       1012    5208
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       6220
 
BstBI TT'CG_AA

Cuts at:   5024    5024 
   Size:       6220
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6220
 
BstYI r'GATC_y

Cuts at:   1972    3514    3550    3917    4302    4319    5771    5857    5869 
   Size:       1542      36     367     385      17    1452      86      12

Cuts at:   5869    6215    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1542    1452     385     367     346      86      36
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2055     513 
   Size:        552     990    4678

  Fragments arranged by size: 

                4678     990     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5481

  Fragments arranged by size: 

                5481     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2179    2259    2958    3736    4530    4550    2179 
   Size:         80     699     778     794      20    3849

  Fragments arranged by size: 

                3849     794     778     699      80      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6220
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6185
 
DraI TTT'AAA

Cuts at:   3524    3524 
   Size:       6220
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5227     809 
   Size:        507    3911    1802

  Fragments arranged by size: 

                3911    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3412    3667    4556 
   Size:         54    1425      50     275      18    1582     255     889

Cuts at:   4556    6185       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1582    1425     889     275     255      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3667    1537 
   Size:        275    1855    4090

  Fragments arranged by size: 

                4090    1855     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3083    4150    1353 
   Size:       1730    1067    3423

  Fragments arranged by size: 

                3423    1730    1067
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2003    4807    5181    5327     387 
   Size:       1064     138     414    2804     374     146    1280

  Fragments arranged by size: 

                2804    1280    1064     414     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2333    2394    2860    2985    3071    3107    3119 
   Size:        393     701      61     466     125      86      36      12

Cuts at:   3119    3260    3357    4283    4880    5667    5935    1239 
   Size:        141      97     926     597     787     268    1524

  Fragments arranged by size: 

                1524     926     787     701     597     466     393     268
                 141     125      97      86      61      36      12
 
EcoRI G'AATT_C

Cuts at:   3529    3907    3529 
   Size:        378    5842
 
EcoRV GAT'ATC

Cuts at:   2303    2303 
   Size:       6220
 
FspI TGC'GCA

Cuts at:   4726    4726 
   Size:       6220
 
HaeII r_GCGC'y

Cuts at:   1678    2027    2045    5367    1678 
   Size:        349      18    3322    2531

  Fragments arranged by size: 

                3322    2531     349      18
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2117    2149    2595    2627    4861 
   Size:         32     135      32     545      32     446      32    2234

Cuts at:   4861    4893    4935    4967    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    2234     545     446     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3564    3810     669 
   Size:        988     281    1626     246    3079

  Fragments arranged by size: 

                3079    1626     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       6220
 
HpaI GTT'AAC

Cuts at:   3810    3810 
   Size:       6220
 
KpnI G_GTAC'C

Cuts at:   3545    3545 
   Size:       6220
 
MfeI C'AATT_G

Cuts at:   3819    3819 
   Size:       6220
 
MluI A'CGCG_T

Cuts at:   6096    6096 
   Size:       6220
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2103    2148    2330    2856    5334    5518    1305 
   Size:        798      45     182     526    2478     184    2007

  Fragments arranged by size: 

                2478    2007     798     526     184     182      45
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4398

  Fragments arranged by size: 

                4398    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2565    4178    4537    4696     518 
   Size:        648    1399    1613     359     159    2042

  Fragments arranged by size: 

                2042    1613    1399     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2057    2072    3636    4313    5461    5706    1606 
   Size:        451      15    1564     677    1148     245    2120

  Fragments arranged by size: 

                2120    1564    1148     677     451     245      15
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5668
 
NdeI CA'TA_TG

Cuts at:    387    3337     387 
   Size:       2950    3270
 
NheI G'CTAG_C

Cuts at:   1052    2909    1052 
   Size:       1857    4363
 
NotI GC'GGCC_GC

Cuts at:   3667    3667 
   Size:       6220
 
NspI r_CATG'y

Cuts at:   1132    1229    3578    5123    1132 
   Size:         97    2349    1545    2229

  Fragments arranged by size: 

                2349    2229    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6220
 
PciI A'CATG_T

Cuts at:   5119    5119 
   Size:       6220
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2838    1108 
   Size:       1730    4490
 
PfoI T'CCnGG_A

Cuts at:   2712    2874    2712 
   Size:        162    6058
 
PmeI GTTT'AAAC

Cuts at:   3524    3524 
   Size:       6220
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4289    4321    5802    5834    4289 
   Size:         32    1481      32    4675

  Fragments arranged by size: 

                4675    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6220
 
PsiI TTA'TAA

Cuts at:   3790    3790 
   Size:       6220
 
PstI C_TGCA'G

Cuts at:    830    2215    3066    3572     830 
   Size:       1385     851     506    3478

  Fragments arranged by size: 

                3478    1385     851     506
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6220
 
SacII CC_GC'GG

Cuts at:   2058    2058 
   Size:       6220
 
SalI G'TCGA_C

Cuts at:   1936    3562    1936 
   Size:       1626    4594
 
SbfI CC_TGCA'GG

Cuts at:   3066    3572    3066 
   Size:        506    5714
 
ScaI AGT'ACT

Cuts at:   4468    6180    4468 
   Size:       1712    4508
 
SfcI C'TryA_G

Cuts at:    826    1045    2211    2469    2904    3062    3568    4703    5384 
   Size:        219    1166     258     435     158     506    1135     681

Cuts at:   5384    5575     826 
   Size:        191    1471

  Fragments arranged by size: 

                1471    1166    1135     681     506     435     258     219
                 191     158
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6220
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6220
 
SmaI CCC'GGG

Cuts at:   1872    2034    3547    1872 
   Size:        162    1513    4545

  Fragments arranged by size: 

                4545    1513     162
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2536    4324    5225    5487     820 
   Size:        197     926     593    1788     901     262    1553

  Fragments arranged by size: 

                1788    1553     926     901     593     262     197
 
SnaBI TAC'GTA

Cuts at:    493    4011     493 
   Size:       3518    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6220
 
SphI G_CATG'C

Cuts at:   1132    3578    1132 
   Size:       2446    3774
 
SspI AAT'ATT

Cuts at:      5      52    4144       5 
   Size:         47    4092    2081

  Fragments arranged by size: 

                4092    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6220
 
StyI C'CwwG_G

Cuts at:    513    1065    2357    3215    3659     513 
   Size:        552    1292     858     444    3074

  Fragments arranged by size: 

                3074    1292     858     552     444
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    4247    4264    4417    4602    5047     325 
   Size:       1255    2667      17     153     185     445    1498

  Fragments arranged by size: 

                2667    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4466    6178      96 
   Size:        274      80      33      51    1443    2489    1712     138

  Fragments arranged by size: 

                2489    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6180    6212    6180 
   Size:         32    6188
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6220
 
XbaI T'CTAG_A

Cuts at:   3556    3556 
   Size:       6220
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6220
 
XmaI C'CCGG_G

Cuts at:   1870    2032    3545    1870 
   Size:        162    1513    4545

  Fragments arranged by size: 

                4545    1513     162
 
XmnI GAAnn'nnTTC

Cuts at:   4349    4349 
   Size:       6220
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4028     276 
   Size:         53      83     186     936    2494    2468

  Fragments arranged by size: 

                2494    2468     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI
     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbsI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI    EcoRI    EcoRV     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI     PstI
     PvuI    SacII     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AloI     ApaI     AscI     BaeI     BbeI    BbvCI    BfrBI   Bpu10I
    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII  EcoICRI    EcoNI
 EcoO109I     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     NsiI     PacI     PmlI    PpuMI   PspOMI    PspXI     PsrI
    PvuII    RsrII     SacI    SanDI     SapI    SexAI     SfiI     SfoI
     SrfI     SwaI     XcmI
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