Restriction map for
pFN21AB5019
(Linear) (Six-base) MAPSORT of: KIBB5019.seq Check: 974 from: 1 to: 1515
KIBB5019.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 325 1515
Size: 325 1190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 389 1102 1114 1515
Size: 328 61 713 12 401
Fragments arranged by size:
713 401 328 61 12
AfeI AGC'GCT
Cuts at: 0 20 1515
Size: 20 1495
ApoI r'AATT_y
Cuts at: 0 430 439 865 1152 1198 1515
Size: 430 9 426 287 46 317
Fragments arranged by size:
430 426 317 287 46 9
AvaI C'yCGr_G
Cuts at: 0 27 1515
Size: 27 1488
AvrII C'CTAG_G
Cuts at: 0 352 1515
Size: 352 1163
BanII G_rGCy'C
Cuts at: 0 130 1241 1515
Size: 130 1111 274
Fragments arranged by size:
1111 274 130
BbsI GAAGACnn'nnnn_
Cuts at: 0 752 1515
Size: 752 763
BlpI GC'TnA_GC
Cuts at: 0 1267 1515
Size: 1267 248
Bme1580I G_kGCm'C
Cuts at: 0 884 1515
Size: 884 631
BmgBI CAC'GTC
Cuts at: 0 78 1515
Size: 78 1437
BmtI G_CTAG'C
Cuts at: 0 908 1515
Size: 908 607
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 519 1041 1515
Size: 519 522 474
Fragments arranged by size:
522 519 474
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 855 980 1066 1255 1352 1515
Size: 855 125 86 189 97 163
Fragments arranged by size:
855 189 163 125 97 86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 552 1515
Size: 552 963
BsaBI GATnn'nnATC
Cuts at: 0 1076 1515
Size: 1076 439
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1228 1258 1515
Size: 1228 30 257
Fragments arranged by size:
1228 257 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1033 1515
Size: 1033 482
BseYI C'CCAG_C
Cuts at: 0 877 1515
Size: 877 638
BsiHKAI G_wGCw'C
Cuts at: 0 955 1515
Size: 955 560
Bsp1286I G_dGCh'C
Cuts at: 0 130 884 955 1241 1515
Size: 130 754 71 286 274
Fragments arranged by size:
754 286 274 130 71
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 325 1515
Size: 325 1190
BsrDI GCAATG_nn'
Cuts at: 0 1125 1515
Size: 1125 390
BstYI r'GATC_y
Cuts at: 0 1509 1515
Size: 1509 6
BtgI C'CryG_G
Cuts at: 0 50 1515
Size: 50 1465
BtsI GCAGTG_nn'
Cuts at: 0 174 254 953 1515
Size: 174 80 699 562
Fragments arranged by size:
699 562 174 80
EaeI y'GGCC_r
Cuts at: 0 1407 1515
Size: 1407 108
EarI CTCTTCn'nnn_
Cuts at: 0 1078 1515
Size: 1078 437
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 389 855 980 1066 1102 1114 1255
Size: 328 61 466 125 86 36 12 141
Cuts at: 1255 1352 1515
Size: 97 163
Fragments arranged by size:
466 328 163 141 125 97 86 61
36 12
EcoRV GAT'ATC
Cuts at: 0 298 1515
Size: 298 1217
HaeII r_GCGC'y
Cuts at: 0 22 40 1515
Size: 22 18 1475
Fragments arranged by size:
1475 22 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 112 144 590 622 1515
Size: 112 32 446 32 893
Fragments arranged by size:
893 446 112 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 143 325 851 1515
Size: 98 45 182 526 664
Fragments arranged by size:
664 526 182 98 45
MslI CAynn'nnrTG
Cuts at: 0 560 1515
Size: 560 955
MspA1I CmG'CkG
Cuts at: 0 52 67 1515
Size: 52 15 1448
Fragments arranged by size:
1448 52 15
NdeI CA'TA_TG
Cuts at: 0 1332 1515
Size: 1332 183
NheI G'CTAG_C
Cuts at: 0 904 1515
Size: 904 611
PflMI CCAn_nnn'nTGG
Cuts at: 0 833 1515
Size: 833 682
PfoI T'CCnGG_A
Cuts at: 0 707 869 1515
Size: 707 162 646
Fragments arranged by size:
707 646 162
PstI C_TGCA'G
Cuts at: 0 210 1061 1515
Size: 210 851 454
Fragments arranged by size:
851 454 210
SacII CC_GC'GG
Cuts at: 0 53 1515
Size: 53 1462
SbfI CC_TGCA'GG
Cuts at: 0 1061 1515
Size: 1061 454
SfcI C'TryA_G
Cuts at: 0 206 464 899 1057 1515
Size: 206 258 435 158 458
Fragments arranged by size:
458 435 258 206 158
SmaI CCC'GGG
Cuts at: 0 29 1515
Size: 29 1486
SmlI C'TyrA_G
Cuts at: 0 531 1515
Size: 531 984
StyI C'CwwG_G
Cuts at: 0 352 1210 1515
Size: 352 858 305
Fragments arranged by size:
858 352 305
XmaI C'CCGG_G
Cuts at: 0 27 1515
Size: 27 1488
Enzymes that do cut:
AarI AcuI AfeI ApoI AvaI AvrII BanII BbsI
BlpI Bme1580I BmgBI BmtI BplI BpmI BpuEI BsaBI
BsaXI BseRI BseYI BsiHKAI Bsp1286I BspMI BsrDI BstYI
BtgI BtsI EaeI EarI Eco57MI EcoRV HaeII Hin4I
MmeI MslI MspA1I NdeI NheI PflMI PfoI PstI
SacII SbfI SfcI SmaI SmlI StyI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BmrI Bpu10I BsaI BsaAI
BsaHI BsaWI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NcoI NgoMIV
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SalI SanDI SapI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmnI ZraI