Restriction map for pFN21AB4033
 (Linear) (Six-base) MAPSORT of: KIBB4033.seq  Check: 4972  from: 1  to: 3786



KIBB4033.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AatII G_ACGT'C

Cuts at:      0    1943    2743    3786 
   Size:       1943     800    1043

  Fragments arranged by size: 

                1943    1043     800
 
AccI GT'mk_AC

Cuts at:      0     397    2644    3786 
   Size:        397    2247    1142

  Fragments arranged by size: 

                2247    1142     397
 
AclI AA'CG_TT

Cuts at:      0    1997    3786 
   Size:       1997    1789
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      59     596     910    1046    1583    2734    3071    3361 
   Size:         59     537     314     136     537    1151     337     290

Cuts at:   3361    3786 
   Size:        425

  Fragments arranged by size: 

                1151     537     537     425     337     314     290     136
                  59
 
AflII C'TTAA_G

Cuts at:      0    1945    3786 
   Size:       1945    1841
 
AgeI A'CCGG_T

Cuts at:      0    2078    3786 
   Size:       2078    1708
 
AleI CACnn'nnGTG

Cuts at:      0     221    3552    3786 
   Size:        221    3331     234

  Fragments arranged by size: 

                3331     234     221
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3598    3786 
   Size:       3598     188
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1385    3534    3786 
   Size:       1385    2149     252

  Fragments arranged by size: 

                2149    1385     252
 
ApoI r'AATT_y

Cuts at:      0      27      70     118    2029    3786 
   Size:         27      43      48    1911    1757

  Fragments arranged by size: 

                1911    1757      48      43      27
 
AvaI C'yCGr_G

Cuts at:      0    2960    3786 
   Size:       2960     826
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1110    1143    3786 
   Size:       1110      33    2643

  Fragments arranged by size: 

                2643    1110      33
 
BamHI G'GATC_C

Cuts at:      0    1543    2084    3786 
   Size:       1543     541    1702

  Fragments arranged by size: 

                1702    1543     541
 
BanI G'GyrC_C

Cuts at:      0     841    1390    2157    3786 
   Size:        841     549     767    1629

  Fragments arranged by size: 

                1629     841     767     549
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     477    2791    2834    3384    3619    3722    3736    3751 
   Size:        477    2314      43     550     235     103      14      15

Cuts at:   3751    3786 
   Size:         35

  Fragments arranged by size: 

                2314     550     477     235     103      43      35      15
                  14
 
BbvCI CC'TCA_GC

Cuts at:      0     989    3786 
   Size:        989    2797
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     464    2543    3786 
   Size:        464    2079    1243

  Fragments arranged by size: 

                2079    1243     464
 
BclI T'GATC_A

Cuts at:      0    2501    2853    3485    3786 
   Size:       2501     352     632     301

  Fragments arranged by size: 

                2501     632     352     301
 
BfrBI ATG'CAT

Cuts at:      0    1439    3786 
   Size:       1439    2347
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2728    3786 
   Size:       2728    1058
 
BglII A'GATC_T

Cuts at:      0    1481    1488    3786 
   Size:       1481       7    2298

  Fragments arranged by size: 

                2298    1481       7
 
Bme1580I G_kGCm'C

Cuts at:      0    1238    1393    3577    3786 
   Size:       1238     155    2184     209

  Fragments arranged by size: 

                2184    1238     209     155
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     307    1311    1395    2798    3786 
   Size:        307    1004      84    1403     988

  Fragments arranged by size: 

                1403    1004     988     307      84
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3310    3786 
   Size:       3310     476
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1221    1993    2791    3786 
   Size:       1221     772     798     995

  Fragments arranged by size: 

                1221     995     798     772
 
Bpu10I CC'TnA_GC

Cuts at:      0     989    1607    2674    2900    3786 
   Size:        989     618    1067     226     886

  Fragments arranged by size: 

                1067     989     886     618     226
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2501    3685    3786 
   Size:       2501    1184     101

  Fragments arranged by size: 

                2501    1184     101
 
BsaAI yAC'GTr

Cuts at:      0    1357    3786 
   Size:       1357    2429
 
BsaHI Gr'CG_yC

Cuts at:      0    1940    2740    3786 
   Size:       1940     800    1046

  Fragments arranged by size: 

                1940    1046     800
 
BsaWI w'CCGG_w

Cuts at:      0    2078    3786 
   Size:       2078    1708
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     660     690    3786 
   Size:        660      30    3096

  Fragments arranged by size: 

                3096     660      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1205    1441    2779    3786 
   Size:       1205     236    1338    1007

  Fragments arranged by size: 

                1338    1205    1007     236
 
BseYI C'CCAG_C

Cuts at:      0    1237    3786 
   Size:       1237    2549
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2480    3786 
   Size:       2480    1306
 
BsmI GAATG_Cn'

Cuts at:      0      94    2399    2666    3543    3786 
   Size:         94    2305     267     877     243

  Fragments arranged by size: 

                2305     877     267     243      94
 
Bsp1286I G_dGCh'C

Cuts at:      0    1238    1393    3577    3786 
   Size:       1238     155    2184     209

  Fragments arranged by size: 

                2184    1238     209     155
 
BsrDI GCAATG_nn'

Cuts at:      0    1667    3786 
   Size:       1667    2119
 
BsrFI r'CCGG_y

Cuts at:      0    2078    3786 
   Size:       2078    1708
 
BsrGI T'GTAC_A

Cuts at:      0    2843    3786 
   Size:       2843     943
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2999    3786 
   Size:       2999     787
 
BstBI TT'CG_AA

Cuts at:      0    2628    3786 
   Size:       2628    1158
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1852    2788    2802    3786 
   Size:       1852     936      14     984

  Fragments arranged by size: 

                1852     984     936      14
 
BstYI r'GATC_y

Cuts at:      0     606     821    1481    1488    1543    1827    2084    2565 
   Size:        606     215     660       7      55     284     257     481

Cuts at:   2565    3184    3214    3786 
   Size:        619      30     572

  Fragments arranged by size: 

                 660     619     606     572     481     284     257     215
                  55      30       7
 
Bsu36I CC'TnA_GG

Cuts at:      0    1279    3786 
   Size:       1279    2507
 
BtgI C'CryG_G

Cuts at:      0     261    1724    1963    3786 
   Size:        261    1463     239    1823

  Fragments arranged by size: 

                1823    1463     261     239
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1430    3786 
   Size:       1430    2356
 
BtsI GCAGTG_nn'

Cuts at:      0    1238    2738    2766    3279    3786 
   Size:       1238    1500      28     513     507

  Fragments arranged by size: 

                1500    1238     513     507      28
 
DraI TTT'AAA

Cuts at:      0    1920    3335    3786 
   Size:       1920    1415     451

  Fragments arranged by size: 

                1920    1415     451
 
DraIII CAC_nnn'GTG

Cuts at:      0    1534    3457    3786 
   Size:       1534    1923     329

  Fragments arranged by size: 

                1923    1534     329
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1000    3786 
   Size:       1000    2786
 
EaeI y'GGCC_r

Cuts at:      0     323     414    1721    1791    3786 
   Size:        323      91    1307      70    1995

  Fragments arranged by size: 

                1995    1307     323      91      70
 
EarI CTCTTCn'nnn_

Cuts at:      0      38    1919    2490    3337    3509    3786 
   Size:         38    1881     571     847     172     277

  Fragments arranged by size: 

                1881     847     571     277     172      38
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1468    2824    3786 
   Size:       1468    1356     962

  Fragments arranged by size: 

                1468    1356     962
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      59     596     910    1046    1221    1583    1993    2734 
   Size:         59     537     314     136     175     362     410     741

Cuts at:   2734    2791    3071    3361    3786 
   Size:         57     280     290     425

  Fragments arranged by size: 

                 741     537     425     410     362     314     290     280
                 175     136      59      57
 
EcoO109I rG'GnC_Cy

Cuts at:      0     869    1305    3786 
   Size:        869     436    2481

  Fragments arranged by size: 

                2481     869     436
 
EcoRV GAT'ATC

Cuts at:      0    2586    3786 
   Size:       2586    1200
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1174    1563    2739    3135    3786 
   Size:       1174     389    1176     396     651

  Fragments arranged by size: 

                1176    1174     651     396     389
 
FspI TGC'GCA

Cuts at:      0    2876    3786 
   Size:       2876     910
 
HaeII r_GCGC'y

Cuts at:      0     911    3786 
   Size:        911    2875
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     379     411     660     692     749     781    1466    1473 
   Size:        379      32     249      32      57      32     685       7

Cuts at:   1473    1498    1505    2575    2607    3786 
   Size:         25       7    1070      32    1179

  Fragments arranged by size: 

                1179    1070     685     379     249      57      32      32
                  32      32      25       7       7
 
HincII GTy'rAC

Cuts at:      0     237     927    1509    3786 
   Size:        237     690     582    2277

  Fragments arranged by size: 

                2277     690     582     237
 
MfeI C'AATT_G

Cuts at:      0     365    2193    3786 
   Size:        365    1828    1593

  Fragments arranged by size: 

                1828    1593     365
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     593    3037    3305    3786 
   Size:        593    2444     268     481

  Fragments arranged by size: 

                2444     593     481     268
 
MscI TGG'CCA

Cuts at:      0     325    1723    1793    3786 
   Size:        325    1398      70    1993

  Fragments arranged by size: 

                1993    1398     325      70
 
MslI CAynn'nnrTG

Cuts at:      0     221     772    2800    2894    3552    3724    3786 
   Size:        221     551    2028      94     658     172      62

  Fragments arranged by size: 

                2028     658     551     221     172      94      62
 
MspA1I CmG'CkG

Cuts at:      0    1241    3786 
   Size:       1241    2545
 
NcoI C'CATG_G

Cuts at:      0     261    1963    3786 
   Size:        261    1702    1823

  Fragments arranged by size: 

                1823    1702     261
 
NdeI CA'TA_TG

Cuts at:      0    3243    3786 
   Size:       3243     543
 
NsiI A_TGCA'T

Cuts at:      0    1441    3786 
   Size:       1441    2345
 
NspI r_CATG'y

Cuts at:      0     554    1599    3786 
   Size:        554    1045    2187

  Fragments arranged by size: 

                2187    1045     554
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     680    1697    2940    3786 
   Size:        680    1017    1243     846

  Fragments arranged by size: 

                1243    1017     846     680
 
PfoI T'CCnGG_A

Cuts at:      0    3296    3786 
   Size:       3296     490
 
PmlI CAC'GTG

Cuts at:      0    1357    3786 
   Size:       1357    2429
 
PpuMI rG'GwC_Cy

Cuts at:      0    1305    3786 
   Size:       1305    2481
 
PsiI TTA'TAA

Cuts at:      0     593    3786 
   Size:        593    3193
 
PstI C_TGCA'G

Cuts at:      0    1662    3468    3786 
   Size:       1662    1806     318

  Fragments arranged by size: 

                1806    1662     318
 
PvuII CAG'CTG

Cuts at:      0    1241    3786 
   Size:       1241    2545
 
SanDI GG'GwC_CC

Cuts at:      0    1305    3786 
   Size:       1305    2481
 
ScaI AGT'ACT

Cuts at:      0    3501    3786 
   Size:       3501     285
 
SfcI C'TryA_G

Cuts at:      0    1658    2645    3464    3526    3786 
   Size:       1658     987     819      62     260

  Fragments arranged by size: 

                1658     987     819     260      62
 
SmlI C'TyrA_G

Cuts at:      0    1945    3786 
   Size:       1945    1841
 
SphI G_CATG'C

Cuts at:      0    1599    3786 
   Size:       1599    2187
 
StuI AGG'CCT

Cuts at:      0     509    3786 
   Size:        509    3277
 
StyI C'CwwG_G

Cuts at:      0     261    1963    3621    3644    3786 
   Size:        261    1702    1658      23     142

  Fragments arranged by size: 

                1702    1658     261     142      23
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2525    3631    3786 
   Size:       2525    1106     155

  Fragments arranged by size: 

                2525    1106     155
 
TatI w'GTAC_w

Cuts at:      0     863    1953    2843    3032    3499    3786 
   Size:        863    1090     890     189     467     287

  Fragments arranged by size: 

                1090     890     863     467     287     189
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1091    1123    3786 
   Size:       1091      32    2663

  Fragments arranged by size: 

                2663    1091      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     833    3786 
   Size:        833    2953
 
ZraI GAC'GTC

Cuts at:      0    1941    2741    3786 
   Size:       1941     800    1045

  Fragments arranged by size: 

                1941    1045     800
 

 Enzymes that do cut:

    AatII     AccI     AclI     AcuI    AflII     AgeI     AleI     AlfI
    AlwNI     ApoI     AvaI     BaeI    BamHI     BanI     BbsI    BbvCI
    BciVI     BclI    BfrBI     BglI    BglII Bme1580I     BmrI     BplI
     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI     BsmI Bsp1286I    BsrDI    BsrFI    BsrGI   BstAPI
    BstBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI
   DraIII     DrdI     EaeI     EarI     EciI  Eco57MI EcoO109I    EcoRV
     FalI     FspI    HaeII    Hin4I   HincII     MfeI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NsiI     NspI    PflMI     PfoI
     PmlI    PpuMI     PsiI     PstI    PvuII    SanDI     ScaI     SfcI
     SmlI     SphI     StuI     StyI    TaqII     TatI     TstI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AfeI   AflIII     AhdI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII    BanII     BbeI     BcgI     BlpI
    BmgBI     BmtI    BpuEI    BsaBI    BsiEI  BsiHKAI    BsiWI    BsmBI
    BspEI    BspHI    BspMI    BsrBI   BssHII    BssSI   BstEII  BstZ17I
     ClaI    CspCI     EagI  EcoICRI    EcoNI    EcoRI     FseI    FspAI
  HindIII     HpaI     KasI     KpnI     MluI     NaeI     NarI   NgoMIV
     NheI     NotI     NruI     PacI     PasI     PciI     PmeI     PpiI
    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI  Tth111I     XbaI
     XcmI     XhoI     XmaI
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