(Circular) (Six-base) MAPSORT of: pFN21AB3125.seq Check: 9456 from: 1 to: 6487 pFN21AB3125 6487 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4297 278 Size: 53 83 186 936 2761 2468 Fragments arranged by size: 2761 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3271 3830 1937 Size: 1334 559 4594 Fragments arranged by size: 4594 1334 559 Acc65I G'GTAC_C Cuts at: 3808 3808 Size: 6487 AclI AA'CG_TT Cuts at: 1563 4614 4987 1563 Size: 3051 373 3063 Fragments arranged by size: 3063 3051 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4550 5934 6202 1632 Size: 2918 1384 268 1917 Fragments arranged by size: 2918 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2693 820 Size: 197 1676 4614 Fragments arranged by size: 4614 1676 197 AflIII A'CryG_T Cuts at: 1203 2640 2772 5386 6363 1203 Size: 1437 132 2614 977 1327 Fragments arranged by size: 2614 1437 1327 977 132 AgeI A'CCGG_T Cuts at: 5318 5318 Size: 6487 AhdI GACnn_n'nnGTC Cuts at: 5216 5216 Size: 6487 AleI CACnn'nnGTG Cuts at: 1166 2414 1166 Size: 1248 5239 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3138 3261 1184 Size: 1954 123 4410 Fragments arranged by size: 4410 1954 123 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2219 2251 2219 Size: 32 6455 AlwNI CAG_nnn'CTG Cuts at: 2282 2446 2509 3784 4230 5802 2282 Size: 164 63 1275 446 1572 2967 Fragments arranged by size: 2967 1572 1275 446 164 63 ApaLI G'TGCA_C Cuts at: 4544 5700 4544 Size: 1156 5331 ApoI r'AATT_y Cuts at: 1493 2985 3500 3796 4022 4174 6283 1493 Size: 1492 515 296 226 152 2109 1697 Fragments arranged by size: 2109 1697 1492 515 296 226 152 AseI AT'TA_AT Cuts at: 160 1032 5041 160 Size: 872 4009 1606 Fragments arranged by size: 4009 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6487 AvaI C'yCGr_G Cuts at: 1870 1943 2489 3812 1870 Size: 73 546 1323 4545 Fragments arranged by size: 4545 1323 546 73 BamHI G'GATC_C Cuts at: 3817 4184 3817 Size: 367 6120 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2759 3808 5263 618 Size: 325 217 627 972 1049 1455 1842 Fragments arranged by size: 1842 1455 1049 972 627 325 217 BanII G_rGCy'C Cuts at: 1391 2139 2478 3806 1391 Size: 748 339 1328 4072 Fragments arranged by size: 4072 1328 748 339 BbeI G_GCGC'C Cuts at: 2763 2763 Size: 6487 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2004 928 Size: 971 105 5411 Fragments arranged by size: 5411 971 105 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2022 2056 2240 2274 4678 4712 1903 Size: 34 85 34 184 34 2404 34 3678 Fragments arranged by size: 3678 2404 184 85 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3296 4379 5589 3296 Size: 1083 1210 4194 Fragments arranged by size: 4194 1210 1083 BclI T'GATC_A Cuts at: 1548 1548 Size: 6487 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5098 136 Size: 107 122 71 631 605 3426 1525 Fragments arranged by size: 3426 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2142 2308 6482 2142 Size: 166 4174 2147 Fragments arranged by size: 4174 2147 166 BlpI GC'TnA_GC Cuts at: 3904 3904 Size: 6487 Bme1580I G_kGCm'C Cuts at: 1790 3422 4548 5704 1790 Size: 1632 1126 1156 2573 Fragments arranged by size: 2573 1632 1156 1126 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6487 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2141 3727 3782 Size: 416 53 83 399 24 724 1586 55 Cuts at: 3782 4218 5176 442 Size: 436 958 1753 Fragments arranged by size: 1753 1586 958 724 436 416 399 83 55 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6487 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2519 2628 5147 1239 Size: 1280 109 2519 2579 Fragments arranged by size: 2579 2519 1280 109 Bpu10I CC'TnA_GC Cuts at: 3528 3528 Size: 6487 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2123 4612 5477 5775 2123 Size: 2489 865 298 2835 Fragments arranged by size: 2835 2489 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5150 882 Size: 617 3651 2219 Fragments arranged by size: 3651 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3379 4278 493 Size: 711 2175 899 2702 Fragments arranged by size: 2702 2175 899 711 BsaBI GATnn'nnATC Cuts at: 3710 6481 3710 Size: 2771 3716 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2760 4294 4676 Size: 53 83 186 936 476 751 1534 382 Cuts at: 4676 5311 275 Size: 635 1451 Fragments arranged by size: 1534 1451 936 751 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3702 4920 5318 5592 5739 6150 3702 Size: 1218 398 274 147 411 4039 Fragments arranged by size: 4039 1218 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2561 2591 1178 Size: 30 165 30 1158 30 5074 Fragments arranged by size: 5074 1158 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2029 2348 2366 3773 1186 Size: 843 319 18 1407 3900 Fragments arranged by size: 3900 1407 843 319 18 BseYI C'CCAG_C Cuts at: 2894 3721 5690 6186 2894 Size: 827 1969 496 3195 Fragments arranged by size: 3195 1969 827 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2886 3318 1163 Size: 573 1150 432 4332 Fragments arranged by size: 4332 1150 573 432 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2254 3937 4698 5726 1540 Size: 275 186 253 1683 761 1028 2301 Fragments arranged by size: 2301 1683 1028 761 275 253 186 BsiHKAI G_wGCw'C Cuts at: 2478 3245 3534 4548 4633 5704 2478 Size: 767 289 1014 85 1071 3261 Fragments arranged by size: 3261 1071 1014 767 289 85 BsiWI C'GTAC_G Cuts at: 2088 2088 Size: 6487 BsmI GAATG_Cn' Cuts at: 3998 4091 3998 Size: 93 6394 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2139 2478 3245 3422 3534 3806 4548 Size: 399 349 339 767 177 112 272 742 Cuts at: 4548 4633 5704 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 767 742 399 349 339 272 177 112 85 BspEI T'CCGG_A Cuts at: 3702 6150 3702 Size: 2448 4039 BspHI T'CATG_A Cuts at: 4376 6106 4376 Size: 1730 4757 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2082 2238 3842 844 Size: 1238 156 1604 3489 Fragments arranged by size: 3489 1604 1238 156 BsrBI CCG'CTC Cuts at: 1394 1823 2054 4374 1394 Size: 429 231 2320 3507 Fragments arranged by size: 3507 2320 429 231 BsrDI GCAATG_nn' Cuts at: 65 1248 2950 4982 5156 65 Size: 1183 1702 2032 174 1396 Fragments arranged by size: 2032 1702 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5131 5318 1700 Size: 110 3321 187 2869 Fragments arranged by size: 3321 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2775 96 Size: 2679 3808 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6487 BssSI C'ACGA_G Cuts at: 4547 5559 4547 Size: 1012 5475 BstAPI GCAn_nnn'nTGC Cuts at: 794 2344 794 Size: 1550 4937 BstBI TT'CG_AA Cuts at: 5291 5291 Size: 6487 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6487 BstXI CCAn_nnnn'nTGG Cuts at: 2131 2173 2419 2587 2131 Size: 42 246 168 6031 Fragments arranged by size: 6031 246 168 42 BstYI r'GATC_y Cuts at: 1972 2142 2308 2505 3781 3817 4184 4569 4586 Size: 170 166 197 1276 36 367 385 17 Cuts at: 4586 6038 6124 6136 6482 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1276 385 367 346 197 170 166 86 36 17 12 BstZ17I GTA'TAC Cuts at: 3272 3272 Size: 6487 Bsu36I CC'TnA_GG Cuts at: 3569 3569 Size: 6487 BtgI C'CryG_G Cuts at: 513 1065 2004 2113 3660 513 Size: 552 939 109 1547 3340 Fragments arranged by size: 3340 1547 939 552 109 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5748 Fragments arranged by size: 5748 683 56 BtsI GCAGTG_nn' Cuts at: 3245 4003 4797 4817 3245 Size: 758 794 20 4915 Fragments arranged by size: 4915 794 758 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6487 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2424 2459 529 Size: 35 1860 35 4557 Fragments arranged by size: 4557 1860 35 35 DraI TTT'AAA Cuts at: 3791 3791 Size: 6487 DraIII CAC_nnn'GTG Cuts at: 3616 3616 Size: 6487 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5494 809 Size: 507 4178 1802 Fragments arranged by size: 4178 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2628 3370 3934 Size: 54 1425 50 275 18 798 742 564 Cuts at: 3934 4823 6452 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 798 742 564 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3934 1537 Size: 275 2122 4090 Fragments arranged by size: 4090 2122 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2172 2177 2861 3314 4417 1353 Size: 819 5 684 453 1103 3423 Fragments arranged by size: 3423 1103 819 684 453 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5074 5448 5594 387 Size: 1064 138 3485 374 146 1280 Fragments arranged by size: 3485 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2476 2476 Size: 6487 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2519 2628 4550 5147 5934 6202 1239 Size: 393 887 109 1922 597 787 268 1524 Fragments arranged by size: 1922 1524 887 787 597 393 268 109 EcoNI CCTnn'n_nnAGG Cuts at: 3755 3755 Size: 6487 EcoRI G'AATT_C Cuts at: 3796 4174 3796 Size: 378 6109 EcoRV GAT'ATC Cuts at: 3385 3385 Size: 6487 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3712 3712 Size: 6487 FspI TGC'GCA Cuts at: 4993 4993 Size: 6487 HaeII r_GCGC'y Cuts at: 1678 2763 5634 1678 Size: 1085 2871 2531 Fragments arranged by size: 2871 2531 1085 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2201 2233 2386 2418 2734 Size: 32 135 32 629 32 153 32 316 Cuts at: 2734 2766 5128 5160 5202 5234 1373 Size: 32 2362 32 42 32 2626 Fragments arranged by size: 2626 2362 629 316 153 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2515 3831 4077 669 Size: 988 281 577 1316 246 3079 Fragments arranged by size: 3079 1316 988 577 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6487 HpaI GTT'AAC Cuts at: 4077 4077 Size: 6487 KasI G'GCGC_C Cuts at: 2759 2759 Size: 6487 KpnI G_GTAC'C Cuts at: 3812 3812 Size: 6487 MfeI C'AATT_G Cuts at: 4086 4086 Size: 6487 MluI A'CGCG_T Cuts at: 6363 6363 Size: 6487 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2695 2793 3741 5601 5785 1305 Size: 1390 98 948 1860 184 2007 Fragments arranged by size: 2007 1860 1390 948 184 98 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4665 Fragments arranged by size: 4665 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2414 3120 4445 4804 4963 518 Size: 648 1248 706 1325 359 159 2042 Fragments arranged by size: 2042 1325 1248 706 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2969 3903 4580 5728 5973 1606 Size: 1363 934 677 1148 245 2120 Fragments arranged by size: 2120 1363 1148 934 677 245 NarI GG'CG_CC Cuts at: 2760 2760 Size: 6487 NcoI C'CATG_G Cuts at: 513 1065 2004 3660 513 Size: 552 939 1656 3340 Fragments arranged by size: 3340 1656 939 552 NdeI CA'TA_TG Cuts at: 387 2650 387 Size: 2263 4224 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6487 NotI GC'GGCC_GC Cuts at: 3934 3934 Size: 6487 NspI r_CATG'y Cuts at: 1132 1229 2644 2776 3119 3845 5390 1132 Size: 97 1415 132 343 726 1545 2229 Fragments arranged by size: 2229 1545 1415 726 343 132 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6487 PciI A'CATG_T Cuts at: 2640 2772 5386 2640 Size: 132 2614 3741 Fragments arranged by size: 3741 2614 132 PflMI CCAn_nnn'nTGG Cuts at: 1108 2446 3784 1108 Size: 1338 1338 3811 Fragments arranged by size: 3811 1338 1338 PfoI T'CCnGG_A Cuts at: 2355 2808 3152 2355 Size: 453 344 5690 Fragments arranged by size: 5690 453 344 PmeI GTTT'AAAC Cuts at: 3791 3791 Size: 6487 PmlI CAC'GTG Cuts at: 3379 3379 Size: 6487 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4556 4588 6069 6101 4556 Size: 32 1481 32 4942 Fragments arranged by size: 4942 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6487 PsiI TTA'TAA Cuts at: 4057 4057 Size: 6487 PstI C_TGCA'G Cuts at: 830 2522 3839 830 Size: 1692 1317 3478 Fragments arranged by size: 3478 1692 1317 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6487 PvuII CAG'CTG Cuts at: 2969 2969 Size: 6487 SacI G_AGCT'C Cuts at: 2478 2478 Size: 6487 SalI G'TCGA_C Cuts at: 1936 3829 1936 Size: 1893 4594 SbfI CC_TGCA'GG Cuts at: 3839 3839 Size: 6487 ScaI AGT'ACT Cuts at: 2804 4735 6447 2804 Size: 1931 1712 2844 Fragments arranged by size: 2844 1931 1712 SexAI A'CCwGG_T Cuts at: 2096 2096 Size: 6487 SfcI C'TryA_G Cuts at: 826 1045 2518 3835 4970 5651 5842 826 Size: 219 1473 1317 1135 681 191 1471 Fragments arranged by size: 1473 1471 1317 1135 681 219 191 SfoI GGC'GCC Cuts at: 2761 2761 Size: 6487 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6487 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6487 SmaI CCC'GGG Cuts at: 1872 3814 1872 Size: 1942 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2138 2693 4591 5492 5754 820 Size: 197 926 195 555 1898 901 262 1553 Fragments arranged by size: 1898 1553 926 901 555 262 197 195 SnaBI TAC'GTA Cuts at: 493 4278 493 Size: 3785 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6487 SphI G_CATG'C Cuts at: 1132 3845 1132 Size: 2713 3774 SspI AAT'ATT Cuts at: 5 52 4411 5 Size: 47 4359 2081 Fragments arranged by size: 4359 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6487 StyI C'CwwG_G Cuts at: 513 1065 2004 2412 3277 3660 3679 3926 513 Size: 552 939 408 865 383 19 247 3074 Fragments arranged by size: 3074 939 865 552 408 383 247 19 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2448 4514 4531 4684 4869 5314 325 Size: 1255 868 2066 17 153 185 445 1498 Fragments arranged by size: 2066 1498 1255 868 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2775 2802 4733 Size: 274 80 33 51 1443 798 27 1931 Cuts at: 4733 6445 96 Size: 1712 138 Fragments arranged by size: 1931 1712 1443 798 274 138 80 51 33 27 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2477 2509 2539 2571 6447 6479 2477 Size: 32 30 32 3876 32 2485 Fragments arranged by size: 3876 2485 32 32 32 30 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6487 XbaI T'CTAG_A Cuts at: 3823 3823 Size: 6487 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2416 2579 2416 Size: 163 6324 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6487 XmaI C'CCGG_G Cuts at: 1870 3812 1870 Size: 1942 4545 XmnI GAAnn'nnTTC Cuts at: 4616 4616 Size: 6487 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4295 276 Size: 53 83 186 936 2761 2468 Fragments arranged by size: 2761 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsmBI EcoO109I FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI