Restriction map for
pFN21AB3125
(Linear) (Six-base) MAPSORT of: KIBB3125.seq Check: 2089 from: 1 to: 1782
KIBB3125.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 0 1266 1782
Size: 1266 516
AflII C'TTAA_G
Cuts at: 0 688 1782
Size: 688 1094
AflIII A'CryG_T
Cuts at: 0 635 767 1782
Size: 635 132 1015
Fragments arranged by size:
1015 635 132
AleI CACnn'nnGTG
Cuts at: 0 409 1782
Size: 409 1373
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1133 1256 1782
Size: 1133 123 526
Fragments arranged by size:
1133 526 123
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 214 246 1782
Size: 214 32 1536
Fragments arranged by size:
1536 214 32
AlwNI CAG_nnn'CTG
Cuts at: 0 277 441 504 1779 1782
Size: 277 164 63 1275 3
Fragments arranged by size:
1275 277 164 63 3
ApoI r'AATT_y
Cuts at: 0 980 1495 1782
Size: 980 515 287
Fragments arranged by size:
980 515 287
AvaI C'yCGr_G
Cuts at: 0 484 1782
Size: 484 1298
BanI G'GyrC_C
Cuts at: 0 754 1782
Size: 754 1028
BanII G_rGCy'C
Cuts at: 0 134 473 1782
Size: 134 339 1309
Fragments arranged by size:
1309 339 134
BbeI G_GCGC'C
Cuts at: 0 758 1782
Size: 758 1024
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 17 51 235 269 1782
Size: 17 34 184 34 1513
Fragments arranged by size:
1513 184 34 34 17
BciVI GTATCCnnnnn_n'
Cuts at: 0 1291 1782
Size: 1291 491
BglII A'GATC_T
Cuts at: 0 137 303 1782
Size: 137 166 1479
Fragments arranged by size:
1479 166 137
Bme1580I G_kGCm'C
Cuts at: 0 1417 1782
Size: 1417 365
BmrI ACTGGGnnnn_n'
Cuts at: 0 136 1722 1777 1782
Size: 136 1586 55 5
Fragments arranged by size:
1586 136 55 5
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 623 1782
Size: 514 109 1159
Fragments arranged by size:
1159 514 109
Bpu10I CC'TnA_GC
Cuts at: 0 1523 1782
Size: 1523 259
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 1782
Size: 118 1664
BsaAI yAC'GTr
Cuts at: 0 1374 1782
Size: 1374 408
BsaBI GATnn'nnATC
Cuts at: 0 1705 1782
Size: 1705 77
BsaHI Gr'CG_yC
Cuts at: 0 4 755 1782
Size: 4 751 1027
Fragments arranged by size:
1027 751 4
BsaWI w'CCGG_w
Cuts at: 0 1697 1782
Size: 1697 85
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 556 586 1782
Size: 556 30 1196
Fragments arranged by size:
1196 556 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 24 343 361 1768 1782
Size: 24 319 18 1407 14
Fragments arranged by size:
1407 319 24 18 14
BseYI C'CCAG_C
Cuts at: 0 889 1716 1782
Size: 889 827 66
Fragments arranged by size:
889 827 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 881 1313 1782
Size: 881 432 469
Fragments arranged by size:
881 469 432
BsiEI CG_ry'CG
Cuts at: 0 249 1782
Size: 249 1533
BsiHKAI G_wGCw'C
Cuts at: 0 473 1240 1529 1782
Size: 473 767 289 253
Fragments arranged by size:
767 473 289 253
BsiWI C'GTAC_G
Cuts at: 0 83 1782
Size: 83 1699
Bsp1286I G_dGCh'C
Cuts at: 0 134 473 1240 1417 1529 1782
Size: 134 339 767 177 112 253
Fragments arranged by size:
767 339 253 177 134 112
BspEI T'CCGG_A
Cuts at: 0 1697 1782
Size: 1697 85
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 77 233 1782
Size: 77 156 1549
Fragments arranged by size:
1549 156 77
BsrBI CCG'CTC
Cuts at: 0 49 1782
Size: 49 1733
BsrDI GCAATG_nn'
Cuts at: 0 945 1782
Size: 945 837
BsrGI T'GTAC_A
Cuts at: 0 770 1782
Size: 770 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 0 339 1782
Size: 339 1443
BstXI CCAn_nnnn'nTGG
Cuts at: 0 126 168 414 582 1782
Size: 126 42 246 168 1200
Fragments arranged by size:
1200 246 168 126 42
BstYI r'GATC_y
Cuts at: 0 137 303 500 1776 1782
Size: 137 166 197 1276 6
Fragments arranged by size:
1276 197 166 137 6
BstZ17I GTA'TAC
Cuts at: 0 1267 1782
Size: 1267 515
Bsu36I CC'TnA_GG
Cuts at: 0 1564 1782
Size: 1564 218
BtgI C'CryG_G
Cuts at: 0 108 1655 1782
Size: 108 1547 127
Fragments arranged by size:
1547 127 108
BtsI GCAGTG_nn'
Cuts at: 0 1240 1782
Size: 1240 542
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 419 454 1782
Size: 419 35 1328
Fragments arranged by size:
1328 419 35
DraIII CAC_nnn'GTG
Cuts at: 0 1611 1782
Size: 1611 171
EaeI y'GGCC_r
Cuts at: 0 623 1365 1782
Size: 623 742 417
Fragments arranged by size:
742 623 417
EarI CTCTTCn'nnn_
Cuts at: 0 167 172 856 1309 1782
Size: 167 5 684 453 473
Fragments arranged by size:
684 473 453 167 5
EcoICRI GAG'CTC
Cuts at: 0 471 1782
Size: 471 1311
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 623 1782
Size: 514 109 1159
Fragments arranged by size:
1159 514 109
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1750 1782
Size: 1750 32
EcoRV GAT'ATC
Cuts at: 0 1380 1782
Size: 1380 402
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1707 1782
Size: 1707 75
HaeII r_GCGC'y
Cuts at: 0 758 1782
Size: 758 1024
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 196 228 381 413 729 761 1782
Size: 196 32 153 32 316 32 1021
Fragments arranged by size:
1021 316 196 153 32 32 32
HincII GTy'rAC
Cuts at: 0 510 1782
Size: 510 1272
KasI G'GCGC_C
Cuts at: 0 754 1782
Size: 754 1028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 690 788 1736 1782
Size: 690 98 948 46
Fragments arranged by size:
948 690 98 46
MslI CAynn'nnrTG
Cuts at: 0 409 1115 1782
Size: 409 706 667
Fragments arranged by size:
706 667 409
MspA1I CmG'CkG
Cuts at: 0 964 1782
Size: 964 818
NarI GG'CG_CC
Cuts at: 0 755 1782
Size: 755 1027
NcoI C'CATG_G
Cuts at: 0 1655 1782
Size: 1655 127
NdeI CA'TA_TG
Cuts at: 0 645 1782
Size: 645 1137
NspI r_CATG'y
Cuts at: 0 639 771 1114 1782
Size: 639 132 343 668
Fragments arranged by size:
668 639 343 132
PciI A'CATG_T
Cuts at: 0 635 767 1782
Size: 635 132 1015
Fragments arranged by size:
1015 635 132
PflMI CCAn_nnn'nTGG
Cuts at: 0 441 1782
Size: 441 1341
PfoI T'CCnGG_A
Cuts at: 0 350 803 1147 1782
Size: 350 453 344 635
Fragments arranged by size:
635 453 350 344
PmlI CAC'GTG
Cuts at: 0 1374 1782
Size: 1374 408
PstI C_TGCA'G
Cuts at: 0 517 1782
Size: 517 1265
PvuII CAG'CTG
Cuts at: 0 964 1782
Size: 964 818
SacI G_AGCT'C
Cuts at: 0 473 1782
Size: 473 1309
ScaI AGT'ACT
Cuts at: 0 799 1782
Size: 799 983
SexAI A'CCwGG_T
Cuts at: 0 91 1782
Size: 91 1691
SfcI C'TryA_G
Cuts at: 0 513 1782
Size: 513 1269
SfoI GGC'GCC
Cuts at: 0 756 1782
Size: 756 1026
SmlI C'TyrA_G
Cuts at: 0 133 688 1782
Size: 133 555 1094
Fragments arranged by size:
1094 555 133
StyI C'CwwG_G
Cuts at: 0 407 1272 1655 1674 1782
Size: 407 865 383 19 108
Fragments arranged by size:
865 407 383 108 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 443 1782
Size: 443 1339
TatI w'GTAC_w
Cuts at: 0 770 797 1782
Size: 770 27 985
Fragments arranged by size:
985 770 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 472 504 534 566 1782
Size: 472 32 30 32 1216
Fragments arranged by size:
1216 472 32 32 30
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 411 574 1782
Size: 411 163 1208
Fragments arranged by size:
1208 411 163
Enzymes that do cut:
AccI AflII AflIII AleI AlfI AloI AlwNI ApoI
AvaI BanI BanII BbeI BcgI BciVI BglII Bme1580I
BmrI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrGI BstAPI BstXI BstYI
BstZ17I Bsu36I BtgI BtsI CspCI DraIII EaeI EarI
EcoICRI Eco57MI EcoNI EcoRV FalI HaeII Hin4I HincII
KasI MmeI MslI MspA1I NarI NcoI NdeI NspI
PciI PflMI PfoI PmlI PstI PvuII SacI ScaI
SexAI SfcI SfoI SmlI StyI TaqII TatI TstI
XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AgeI AhdI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BbvCI BclI BfrBI BglI BlpI BmgBI BmtI
BplI BsaI BsmI BsmBI BspHI BsrFI BssHII BssSI
BstBI BstEII BtgZI ClaI DraI DrdI EagI EciI
EcoO109I EcoRI FseI FspI FspAI HindIII HpaI KpnI
MfeI MluI MscI NaeI NgoMIV NheI NotI NruI
NsiI PacI PasI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI
XmaI XmnI ZraI