Restriction map for
pFN21AB9104
(Linear) (Six-base) MAPSORT of: KIBB9104.seq Check: 5152 from: 1 to: 1623
KIBB9104.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AatII G_ACGT'C
Cuts at: 0 1199 1623
Size: 1199 424
AclI AA'CG_TT
Cuts at: 0 427 1623
Size: 427 1196
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1084 1623
Size: 1084 539
AflIII A'CryG_T
Cuts at: 0 1252 1623
Size: 1252 371
AleI CACnn'nnGTG
Cuts at: 0 1108 1623
Size: 1108 515
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1126 1623
Size: 1126 497
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 331 363 1623
Size: 331 32 1260
Fragments arranged by size:
1260 331 32
AlwNI CAG_nnn'CTG
Cuts at: 0 828 1623
Size: 828 795
AvaI C'yCGr_G
Cuts at: 0 683 1623
Size: 683 940
BamHI G'GATC_C
Cuts at: 0 25 1086 1623
Size: 25 1061 537
Fragments arranged by size:
1061 537 25
BanI G'GyrC_C
Cuts at: 0 1102 1110 1602 1623
Size: 1102 8 492 21
Fragments arranged by size:
1102 492 21 8
BanII G_rGCy'C
Cuts at: 0 260 575 632 812 1041 1562 1595 1623
Size: 260 315 57 180 229 521 33 28
Fragments arranged by size:
521 315 260 229 180 57 33 28
BglI GCCn_nnn'nGGC
Cuts at: 0 46 238 1623
Size: 46 192 1385
Fragments arranged by size:
1385 192 46
BlpI GC'TnA_GC
Cuts at: 0 212 488 1623
Size: 212 276 1135
Fragments arranged by size:
1135 276 212
Bme1580I G_kGCm'C
Cuts at: 0 1115 1508 1623
Size: 1115 393 115
Fragments arranged by size:
1115 393 115
BmrI ACTGGGnnnn_n'
Cuts at: 0 29 1590 1623
Size: 29 1561 33
Fragments arranged by size:
1561 33 29
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 709 1623
Size: 709 914
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 166 190 235 298 727 850 886
Size: 151 15 24 45 63 429 123 36
Cuts at: 886 1237 1438 1546 1561 1623
Size: 351 201 108 15 62
Fragments arranged by size:
429 351 201 151 123 108 63 62
45 36 24 15 15
Bpu10I CC'TnA_GC
Cuts at: 0 1040 1623
Size: 1040 583
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 1623
Size: 589 1034
BsaI GGTCTCn'nnnn_
Cuts at: 0 1381 1623
Size: 1381 242
BsaAI yAC'GTr
Cuts at: 0 1255 1359 1623
Size: 1255 104 264
Fragments arranged by size:
1255 264 104
BsaBI GATnn'nnATC
Cuts at: 0 1091 1623
Size: 1091 532
BsaHI Gr'CG_yC
Cuts at: 0 1196 1623
Size: 1196 427
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 847 1000 1489 1623
Size: 148 699 153 489 134
Fragments arranged by size:
699 489 153 148 134
BseYI C'CCAG_C
Cuts at: 0 325 616 805 817 1204 1285 1613 1623
Size: 325 291 189 12 387 81 328 10
Fragments arranged by size:
387 328 325 291 189 81 12 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 1623
Size: 199 1424
BsiEI CG_ry'CG
Cuts at: 0 1132 1343 1623
Size: 1132 211 280
Fragments arranged by size:
1132 280 211
BsiHKAI G_wGCw'C
Cuts at: 0 575 1562 1623
Size: 575 987 61
Fragments arranged by size:
987 575 61
BsmI GAATG_Cn'
Cuts at: 0 464 954 1623
Size: 464 490 669
Fragments arranged by size:
669 490 464
BsmBI CGTCTCn'nnnn_
Cuts at: 0 130 274 1455 1623
Size: 130 144 1181 168
Fragments arranged by size:
1181 168 144 130
Bsp1286I G_dGCh'C
Cuts at: 0 260 575 632 812 1041 1115 1508 1562
Size: 260 315 57 180 229 74 393 54
Cuts at: 1562 1595 1623
Size: 33 28
Fragments arranged by size:
393 315 260 229 180 74 57 54
33 28
BspHI T'CATG_A
Cuts at: 0 966 1623
Size: 966 657
BsrBI CCG'CTC
Cuts at: 0 1146 1623
Size: 1146 477
BsrDI GCAATG_nn'
Cuts at: 0 588 1623
Size: 588 1035
BssHII G'CGCG_C
Cuts at: 0 460 1623
Size: 460 1163
BstAPI GCAn_nnn'nTGC
Cuts at: 0 498 1623
Size: 498 1125
BstYI r'GATC_y
Cuts at: 0 25 103 185 608 1086 1623
Size: 25 78 82 423 478 537
Fragments arranged by size:
537 478 423 82 78 25
BtgI C'CryG_G
Cuts at: 0 41 1106 1176 1571 1623
Size: 41 1065 70 395 52
Fragments arranged by size:
1065 395 70 52 41
BtsI GCAGTG_nn'
Cuts at: 0 555 562 600 807 1623
Size: 555 7 38 207 816
Fragments arranged by size:
816 555 207 38 7
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 570 605 1623
Size: 570 35 1018
Fragments arranged by size:
1018 570 35
EaeI y'GGCC_r
Cuts at: 0 110 1129 1340 1623
Size: 110 1019 211 283
Fragments arranged by size:
1019 283 211 110
EagI C'GGCC_G
Cuts at: 0 1129 1340 1623
Size: 1129 211 283
Fragments arranged by size:
1129 283 211
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1000 1468 1623
Size: 1000 468 155
Fragments arranged by size:
1000 468 155
EcoICRI GAG'CTC
Cuts at: 0 573 1560 1623
Size: 573 987 63
Fragments arranged by size:
987 573 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 166 190 235 298 727 850 886
Size: 151 15 24 45 63 429 123 36
Cuts at: 886 1084 1237 1438 1546 1561 1623
Size: 198 153 201 108 15 62
Fragments arranged by size:
429 201 198 153 151 123 108 63
62 45 36 24 15 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 112 1623
Size: 112 1511
EcoO109I rG'GnC_Cy
Cuts at: 0 195 861 887 1374 1547 1623
Size: 195 666 26 487 173 76
Fragments arranged by size:
666 487 195 173 76 26
FspI TGC'GCA
Cuts at: 0 382 580 1623
Size: 382 198 1043
Fragments arranged by size:
1043 382 198
FspAI rTGC'GCAy
Cuts at: 0 382 1623
Size: 382 1241
HaeII r_GCGC'y
Cuts at: 0 1212 1623
Size: 1212 411
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1023 1055 1623
Size: 1023 32 568
Fragments arranged by size:
1023 568 32
HincII GTy'rAC
Cuts at: 0 1311 1623
Size: 1311 312
MscI TGG'CCA
Cuts at: 0 112 1623
Size: 112 1511
MslI CAynn'nnrTG
Cuts at: 0 419 509 1108 1623
Size: 419 90 599 515
Fragments arranged by size:
599 515 419 90
MspA1I CmG'CkG
Cuts at: 0 207 825 1494 1613 1623
Size: 207 618 669 119 10
Fragments arranged by size:
669 618 207 119 10
NcoI C'CATG_G
Cuts at: 0 1571 1623
Size: 1571 52
NspI r_CATG'y
Cuts at: 0 508 785 1002 1623
Size: 508 277 217 621
Fragments arranged by size:
621 508 277 217
PflMI CCAn_nnn'nTGG
Cuts at: 0 773 828 1623
Size: 773 55 795
Fragments arranged by size:
795 773 55
PfoI T'CCnGG_A
Cuts at: 0 11 251 275 1623
Size: 11 240 24 1348
Fragments arranged by size:
1348 240 24 11
PpuMI rG'GwC_Cy
Cuts at: 0 195 861 887 1547 1623
Size: 195 666 26 660 76
Fragments arranged by size:
666 660 195 76 26
PstI C_TGCA'G
Cuts at: 0 54 569 1623
Size: 54 515 1054
Fragments arranged by size:
1054 515 54
PvuII CAG'CTG
Cuts at: 0 207 825 1613 1623
Size: 207 618 788 10
Fragments arranged by size:
788 618 207 10
SacI G_AGCT'C
Cuts at: 0 575 1562 1623
Size: 575 987 61
Fragments arranged by size:
987 575 61
SanDI GG'GwC_CC
Cuts at: 0 887 1623
Size: 887 736
SfcI C'TryA_G
Cuts at: 0 50 565 1623
Size: 50 515 1058
Fragments arranged by size:
1058 515 50
SmlI C'TyrA_G
Cuts at: 0 604 1623
Size: 604 1019
SphI G_CATG'C
Cuts at: 0 785 1002 1623
Size: 785 217 621
Fragments arranged by size:
785 621 217
StuI AGG'CCT
Cuts at: 0 497 716 1623
Size: 497 219 907
Fragments arranged by size:
907 497 219
StyI C'CwwG_G
Cuts at: 0 113 1571 1623
Size: 113 1458 52
Fragments arranged by size:
1458 113 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 409 499 1590 1605 1623
Size: 409 90 1091 15 18
Fragments arranged by size:
1091 409 90 18 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 253 285 1623
Size: 253 32 1338
Fragments arranged by size:
1338 253 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 333 825 1272 1623
Size: 333 492 447 351
Fragments arranged by size:
492 447 351 333
ZraI GAC'GTC
Cuts at: 0 1197 1623
Size: 1197 426
Enzymes that do cut:
AatII AclI AcuI AflIII AleI AlfI AloI AlwNI
AvaI BamHI BanI BanII BglI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BsrBI BsrDI BssHII BstAPI BstYI BtgI BtsI
CspCI EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I
FspI FspAI HaeII Hin4I HincII MscI MslI MspA1I
NcoI NspI PflMI PfoI PpuMI PstI PvuII SacI
SanDI SfcI SmlI SphI StuI StyI TaqII TstI
XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AfeI AflII AgeI AhdI ApaI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI
BmtI BsaWI BsaXI BsiWI BspEI BspMI BsrFI BsrGI
BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
DraI DraIII DrdI EarI EcoRI EcoRV FalI FseI
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI SwaI TatI Tth111I XbaI XhoI
XmaI XmnI