(Circular) (Six-base) MAPSORT of: pFN21AB3103.seq Check: 9474 from: 1 to: 6355 pFN21AB3103 6355 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2335 2335 Size: 6355 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4165 278 Size: 53 83 186 936 2629 2468 Fragments arranged by size: 2629 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2511 3698 1937 Size: 574 1187 4594 Fragments arranged by size: 4594 1187 574 Acc65I G'GTAC_C Cuts at: 3676 3676 Size: 6355 AclI AA'CG_TT Cuts at: 1563 4482 4855 1563 Size: 2919 373 3063 Fragments arranged by size: 3063 2919 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2001 4418 5802 6070 1632 Size: 369 2417 1384 268 1917 Fragments arranged by size: 2417 1917 1384 369 268 AflII C'TTAA_G Cuts at: 820 1017 2210 820 Size: 197 1193 4965 Fragments arranged by size: 4965 1193 197 AflIII A'CryG_T Cuts at: 1203 5254 6231 1203 Size: 4051 977 1327 Fragments arranged by size: 4051 1327 977 AgeI A'CCGG_T Cuts at: 5186 5186 Size: 6355 AhdI GACnn_n'nnGTC Cuts at: 5084 5084 Size: 6355 AleI CACnn'nnGTG Cuts at: 1166 2990 1166 Size: 1824 4531 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6355 AlwNI CAG_nnn'CTG Cuts at: 3020 3388 3407 4098 5670 3020 Size: 368 19 691 1572 3705 Fragments arranged by size: 3705 1572 691 368 19 ApaLI G'TGCA_C Cuts at: 4412 5568 4412 Size: 1156 5199 ApoI r'AATT_y Cuts at: 1493 2264 2708 2819 3664 3890 4042 6151 1493 Size: 771 444 111 845 226 152 2109 1697 Fragments arranged by size: 2109 1697 845 771 444 226 152 111 AseI AT'TA_AT Cuts at: 160 1032 4909 160 Size: 872 3877 1606 Fragments arranged by size: 3877 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6355 AvaI C'yCGr_G Cuts at: 1870 1943 3253 3430 3680 1870 Size: 73 1310 177 250 4545 Fragments arranged by size: 4545 1310 250 177 73 BamHI G'GATC_C Cuts at: 3685 4052 3685 Size: 367 5988 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2123 2148 3676 5131 618 Size: 325 217 627 336 25 1528 1455 1842 Fragments arranged by size: 1842 1528 1455 627 336 325 217 25 BanII G_rGCy'C Cuts at: 1391 2144 3127 3260 3336 3624 3674 1391 Size: 753 983 133 76 288 50 4072 Fragments arranged by size: 4072 983 753 288 133 76 50 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2441 3017 928 Size: 971 542 576 4266 Fragments arranged by size: 4266 971 576 542 BbvCI CC'TCA_GC Cuts at: 2177 3228 2177 Size: 1051 5304 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2930 2964 4546 4580 1903 Size: 34 993 34 1582 34 3678 Fragments arranged by size: 3678 1582 993 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4247 5457 4247 Size: 1210 5145 BclI T'GATC_A Cuts at: 1548 2401 1548 Size: 853 5502 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3384 3404 4966 Size: 107 122 71 631 605 1712 20 1562 Cuts at: 4966 136 Size: 1525 Fragments arranged by size: 1712 1562 1525 631 605 122 107 71 20 BglII A'GATC_T Cuts at: 3534 6350 3534 Size: 2816 3539 BlpI GC'TnA_GC Cuts at: 3296 3772 3296 Size: 476 5879 Bme1580I G_kGCm'C Cuts at: 1790 2151 2746 4416 5572 1790 Size: 361 595 1670 1156 2573 Fragments arranged by size: 2573 1670 1156 595 361 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6355 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2077 4086 5044 Size: 416 53 83 399 24 660 2009 958 Cuts at: 5044 442 Size: 1753 Fragments arranged by size: 2009 1753 958 660 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6355 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2452 3197 5015 1239 Size: 1213 745 1818 2579 Fragments arranged by size: 2579 1818 1213 745 Bpu10I CC'TnA_GC Cuts at: 2177 3078 3228 2177 Size: 901 150 5304 Fragments arranged by size: 5304 901 150 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4480 5345 5643 4480 Size: 865 298 5192 Fragments arranged by size: 5192 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2066 5018 882 Size: 617 567 2952 2219 Fragments arranged by size: 2952 2219 617 567 BsaAI yAC'GTr Cuts at: 493 1204 4146 493 Size: 711 2942 2702 Fragments arranged by size: 2942 2702 711 BsaBI GATnn'nnATC Cuts at: 6349 6349 Size: 6355 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4162 4544 5179 275 Size: 53 83 186 936 2629 382 635 1451 Fragments arranged by size: 2629 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4788 5186 5460 5607 6018 4788 Size: 398 274 147 411 5125 Fragments arranged by size: 5125 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3323 3353 1178 Size: 30 165 30 1920 30 4180 Fragments arranged by size: 4180 1920 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3194 3254 1186 Size: 2008 60 4287 Fragments arranged by size: 4287 2008 60 BseYI C'CCAG_C Cuts at: 2751 2898 3126 3628 5558 6054 2751 Size: 147 228 502 1930 496 3052 Fragments arranged by size: 3052 1930 502 496 228 147 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2156 2516 2738 2960 1163 Size: 573 420 360 222 222 4558 Fragments arranged by size: 4558 573 420 360 222 222 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2506 3805 4566 5594 1540 Size: 275 186 505 1299 761 1028 2301 Fragments arranged by size: 2301 1299 1028 761 505 275 186 BsiHKAI G_wGCw'C Cuts at: 4416 4501 5572 4416 Size: 85 1071 5199 Fragments arranged by size: 5199 1071 85 BsmI GAATG_Cn' Cuts at: 2968 3866 3959 2968 Size: 898 93 5364 Fragments arranged by size: 5364 898 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2144 2151 2746 3127 3260 3336 3624 Size: 399 354 7 595 381 133 76 288 Cuts at: 3624 3674 4416 4501 5572 1391 Size: 50 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 595 399 381 354 288 133 85 76 50 7 BspEI T'CCGG_A Cuts at: 6018 6018 Size: 6355 BspHI T'CATG_A Cuts at: 4244 5974 4244 Size: 1730 4625 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2335 3643 3710 844 Size: 1491 1308 67 3489 Fragments arranged by size: 3489 1491 1308 67 BsrBI CCG'CTC Cuts at: 1394 1823 3145 4242 1394 Size: 429 1322 1097 3507 Fragments arranged by size: 3507 1322 1097 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4850 5024 65 Size: 1183 3602 174 1396 Fragments arranged by size: 3602 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3612 4999 5186 1700 Size: 110 1802 1387 187 2869 Fragments arranged by size: 2869 1802 1387 187 110 BsrGI T'GTAC_A Cuts at: 96 3339 96 Size: 3243 3112 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6355 BssSI C'ACGA_G Cuts at: 2558 2669 2891 4415 5427 2558 Size: 111 222 1524 1012 3486 Fragments arranged by size: 3486 1524 1012 222 111 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6355 BstBI TT'CG_AA Cuts at: 5159 5159 Size: 6355 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6355 BstXI CCAn_nnnn'nTGG Cuts at: 2255 3640 2255 Size: 1385 4970 BstYI r'GATC_y Cuts at: 1972 3534 3685 4052 4437 4454 5906 5992 6004 Size: 1562 151 367 385 17 1452 86 12 Cuts at: 6004 6350 1972 Size: 346 1977 Fragments arranged by size: 1977 1562 1452 385 367 346 151 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2248 2359 513 Size: 552 1183 111 4509 Fragments arranged by size: 4509 1183 552 111 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2023 3540 487 Size: 683 56 797 1517 3302 Fragments arranged by size: 3302 1517 797 683 56 BtsI GCAGTG_nn' Cuts at: 2335 3871 4665 4685 2335 Size: 1536 794 20 4005 Fragments arranged by size: 4005 1536 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6355 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6320 DraI TTT'AAA Cuts at: 3659 3659 Size: 6355 DraIII CAC_nnn'GTG Cuts at: 2032 2032 Size: 6355 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5362 809 Size: 507 4046 1802 Fragments arranged by size: 4046 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2305 2883 3135 Size: 54 1425 50 275 18 475 578 252 Cuts at: 3135 3802 4691 6320 8 Size: 667 889 1629 43 Fragments arranged by size: 1629 1425 889 667 578 475 275 252 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3802 1537 Size: 275 1990 4090 Fragments arranged by size: 4090 1990 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3153 3629 4285 1353 Size: 1800 476 656 3423 Fragments arranged by size: 3423 1800 656 476 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3118 3125 3131 4942 5316 5462 Size: 1064 138 1529 7 6 1811 374 146 Cuts at: 5462 387 Size: 1280 Fragments arranged by size: 1811 1529 1280 1064 374 146 138 7 6 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2001 2452 3197 4418 5015 5802 6070 Size: 393 369 451 745 1221 597 787 268 Cuts at: 6070 1239 Size: 1524 Fragments arranged by size: 1524 1221 787 745 597 451 393 369 268 EcoNI CCTnn'n_nnAGG Cuts at: 3077 3077 Size: 6355 EcoO109I rG'GnC_Cy Cuts at: 3308 3385 3427 3308 Size: 77 42 6236 Fragments arranged by size: 6236 77 42 EcoRI G'AATT_C Cuts at: 3664 4042 3664 Size: 378 5977 FspI TGC'GCA Cuts at: 4861 4861 Size: 6355 HaeII r_GCGC'y Cuts at: 1678 2634 5502 1678 Size: 956 2868 2531 Fragments arranged by size: 2868 2531 956 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3521 3553 4996 5028 5070 Size: 32 135 32 1949 32 1443 32 42 Cuts at: 5070 5102 1373 Size: 32 2626 Fragments arranged by size: 2626 1949 1443 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2970 3699 3945 669 Size: 988 281 1032 729 246 3079 Fragments arranged by size: 3079 1032 988 729 281 246 HindIII A'AGCT_T Cuts at: 748 2013 2241 748 Size: 1265 228 4862 Fragments arranged by size: 4862 1265 228 HpaI GTT'AAC Cuts at: 3945 3945 Size: 6355 KpnI G_GTAC'C Cuts at: 3680 3680 Size: 6355 MfeI C'AATT_G Cuts at: 3954 3954 Size: 6355 MluI A'CGCG_T Cuts at: 6231 6231 Size: 6355 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2814 5469 5653 1305 Size: 1509 2655 184 2007 Fragments arranged by size: 2655 2007 1509 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2885 3137 10 Size: 54 1425 343 1053 252 3228 Fragments arranged by size: 3228 1425 1053 343 252 54 MslI CAynn'nnrTG Cuts at: 518 1166 2253 2990 3572 4313 4672 4831 518 Size: 648 1087 737 582 741 359 159 2042 Fragments arranged by size: 2042 1087 741 737 648 582 359 159 MspA1I CmG'CkG Cuts at: 1606 2300 2776 2852 3130 3356 3771 4448 5596 Size: 694 476 76 278 226 415 677 1148 Cuts at: 5596 5841 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 694 677 476 415 278 245 226 76 NcoI C'CATG_G Cuts at: 513 1065 2248 513 Size: 552 1183 4620 Fragments arranged by size: 4620 1183 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6355 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6355 NotI GC'GGCC_GC Cuts at: 3802 3802 Size: 6355 NspI r_CATG'y Cuts at: 1132 1229 2450 3713 5258 1132 Size: 97 1221 1263 1545 2229 Fragments arranged by size: 2229 1545 1263 1221 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6355 PciI A'CATG_T Cuts at: 5254 5254 Size: 6355 PflMI CCAn_nnn'nTGG Cuts at: 1108 3133 1108 Size: 2025 4330 PmeI GTTT'AAAC Cuts at: 3659 3659 Size: 6355 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4424 4456 5937 5969 4424 Size: 32 1481 32 4810 Fragments arranged by size: 4810 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6355 PsiI TTA'TAA Cuts at: 2799 3925 2799 Size: 1126 5229 PspXI vC'TCGA_Gb Cuts at: 3253 3253 Size: 6355 PstI C_TGCA'G Cuts at: 830 3707 830 Size: 2877 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6355 PvuII CAG'CTG Cuts at: 2300 2776 2300 Size: 476 5879 RsrII CG'GwC_CG Cuts at: 2356 2356 Size: 6355 SalI G'TCGA_C Cuts at: 1936 3697 1936 Size: 1761 4594 SapI GCTCTTCn'nnn_ Cuts at: 3629 3629 Size: 6355 SbfI CC_TGCA'GG Cuts at: 3707 3707 Size: 6355 ScaI AGT'ACT Cuts at: 4603 6315 4603 Size: 1712 4643 SfcI C'TryA_G Cuts at: 826 1045 2412 3301 3703 4838 5519 5710 826 Size: 219 1367 889 402 1135 681 191 1471 Fragments arranged by size: 1471 1367 1135 889 681 402 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6355 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6355 SmaI CCC'GGG Cuts at: 1872 3432 3682 1872 Size: 1560 250 4545 Fragments arranged by size: 4545 1560 250 SmlI C'TyrA_G Cuts at: 820 1017 1943 2210 3253 4459 5360 5622 820 Size: 197 926 267 1043 1206 901 262 1553 Fragments arranged by size: 1553 1206 1043 926 901 267 262 197 SnaBI TAC'GTA Cuts at: 493 4146 493 Size: 3653 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6355 SphI G_CATG'C Cuts at: 1132 2450 3713 1132 Size: 1318 1263 3774 Fragments arranged by size: 3774 1318 1263 SspI AAT'ATT Cuts at: 5 52 4279 5 Size: 47 4227 2081 Fragments arranged by size: 4227 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6355 StyI C'CwwG_G Cuts at: 513 1065 2248 2746 3138 3794 513 Size: 552 1183 498 392 656 3074 Fragments arranged by size: 3074 1183 656 552 498 392 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2090 2298 2344 2520 3002 4382 4399 Size: 1255 510 208 46 176 482 1380 17 Cuts at: 4399 4552 4737 5182 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1380 1255 510 482 445 208 185 176 153 46 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3339 4601 6313 Size: 274 80 33 51 1443 1362 1262 1712 Cuts at: 6313 96 Size: 138 Fragments arranged by size: 1712 1443 1362 1262 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2018 2050 2254 2286 6315 6347 2018 Size: 32 204 32 4029 32 2026 Fragments arranged by size: 4029 2026 204 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2354 1316 Size: 1038 5317 XbaI T'CTAG_A Cuts at: 3691 3691 Size: 6355 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3172 3172 Size: 6355 XhoI C'TCGA_G Cuts at: 1943 3253 1943 Size: 1310 5045 XmaI C'CCGG_G Cuts at: 1870 3430 3680 1870 Size: 1560 250 4545 Fragments arranged by size: 4545 1560 250 XmnI GAAnn'nnTTC Cuts at: 4484 4484 Size: 6355 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4163 276 Size: 53 83 186 936 2629 2468 Fragments arranged by size: 2629 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII RsrII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI