Restriction map for
pFN21AB3103
(Linear) (Six-base) MAPSORT of: KIBB3103.seq Check: 8446 from: 1 to: 1650
KIBB3103.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 330 1650
Size: 330 1320
AccI GT'mk_AC
Cuts at: 0 506 1650
Size: 506 1144
AflII C'TTAA_G
Cuts at: 0 205 1650
Size: 205 1445
AleI CACnn'nnGTG
Cuts at: 0 985 1650
Size: 985 665
AlwNI CAG_nnn'CTG
Cuts at: 0 1015 1383 1402 1650
Size: 1015 368 19 248
Fragments arranged by size:
1015 368 248 19
ApoI r'AATT_y
Cuts at: 0 259 703 814 1650
Size: 259 444 111 836
Fragments arranged by size:
836 444 259 111
AvaI C'yCGr_G
Cuts at: 0 1248 1425 1650
Size: 1248 177 225
Fragments arranged by size:
1248 225 177
BanI G'GyrC_C
Cuts at: 0 118 143 1650
Size: 118 25 1507
Fragments arranged by size:
1507 118 25
BanII G_rGCy'C
Cuts at: 0 139 1122 1255 1331 1619 1650
Size: 139 983 133 76 288 31
Fragments arranged by size:
983 288 139 133 76 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 436 1012 1650
Size: 436 576 638
Fragments arranged by size:
638 576 436
BbvCI CC'TCA_GC
Cuts at: 0 172 1223 1650
Size: 172 1051 427
Fragments arranged by size:
1051 427 172
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 925 959 1650
Size: 925 34 691
Fragments arranged by size:
925 691 34
BclI T'GATC_A
Cuts at: 0 396 1650
Size: 396 1254
BglI GCCn_nnn'nGGC
Cuts at: 0 1379 1399 1650
Size: 1379 20 251
Fragments arranged by size:
1379 251 20
BglII A'GATC_T
Cuts at: 0 1529 1650
Size: 1529 121
BlpI GC'TnA_GC
Cuts at: 0 1291 1650
Size: 1291 359
Bme1580I G_kGCm'C
Cuts at: 0 146 741 1650
Size: 146 595 909
Fragments arranged by size:
909 595 146
BmrI ACTGGGnnnn_n'
Cuts at: 0 72 1650
Size: 72 1578
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 447 1192 1650
Size: 447 745 458
Fragments arranged by size:
745 458 447
Bpu10I CC'TnA_GC
Cuts at: 0 172 1073 1223 1650
Size: 172 901 150 427
Fragments arranged by size:
901 427 172 150
BsaI GGTCTCn'nnnn_
Cuts at: 0 61 1650
Size: 61 1589
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1318 1348 1650
Size: 1318 30 302
Fragments arranged by size:
1318 302 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1189 1249 1650
Size: 1189 60 401
Fragments arranged by size:
1189 401 60
BseYI C'CCAG_C
Cuts at: 0 746 893 1121 1623 1650
Size: 746 147 228 502 27
Fragments arranged by size:
746 502 228 147 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 511 733 955 1650
Size: 151 360 222 222 695
Fragments arranged by size:
695 360 222 222 151
BsiEI CG_ry'CG
Cuts at: 0 501 1650
Size: 501 1149
BsmI GAATG_Cn'
Cuts at: 0 963 1650
Size: 963 687
Bsp1286I G_dGCh'C
Cuts at: 0 139 146 741 1122 1255 1331 1619 1650
Size: 139 7 595 381 133 76 288 31
Fragments arranged by size:
595 381 288 139 133 76 31 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 330 1650
Size: 330 1320
BsrBI CCG'CTC
Cuts at: 0 1140 1650
Size: 1140 510
BsrFI r'CCGG_y
Cuts at: 0 1607 1650
Size: 1607 43
BsrGI T'GTAC_A
Cuts at: 0 1334 1650
Size: 1334 316
BssSI C'ACGA_G
Cuts at: 0 553 664 886 1650
Size: 553 111 222 764
Fragments arranged by size:
764 553 222 111
BstXI CCAn_nnnn'nTGG
Cuts at: 0 250 1635 1650
Size: 250 1385 15
Fragments arranged by size:
1385 250 15
BstYI r'GATC_y
Cuts at: 0 1529 1650
Size: 1529 121
BtgI C'CryG_G
Cuts at: 0 243 354 1650
Size: 243 111 1296
Fragments arranged by size:
1296 243 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 18 1535 1650
Size: 18 1517 115
Fragments arranged by size:
1517 115 18
BtsI GCAGTG_nn'
Cuts at: 0 330 1650
Size: 330 1320
DraIII CAC_nnn'GTG
Cuts at: 0 27 1650
Size: 27 1623
EaeI y'GGCC_r
Cuts at: 0 300 878 1130 1650
Size: 300 578 252 520
Fragments arranged by size:
578 520 300 252
EarI CTCTTCn'nnn_
Cuts at: 0 1148 1624 1650
Size: 1148 476 26
Fragments arranged by size:
1148 476 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1113 1120 1126 1650
Size: 1113 7 6 524
Fragments arranged by size:
1113 524 7 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 447 1192 1650
Size: 447 745 458
Fragments arranged by size:
745 458 447
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1072 1650
Size: 1072 578
EcoO109I rG'GnC_Cy
Cuts at: 0 1303 1380 1422 1650
Size: 1303 77 42 228
Fragments arranged by size:
1303 228 77 42
HaeII r_GCGC'y
Cuts at: 0 629 1650
Size: 629 1021
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1516 1548 1650
Size: 1516 32 102
Fragments arranged by size:
1516 102 32
HincII GTy'rAC
Cuts at: 0 965 1650
Size: 965 685
HindIII A'AGCT_T
Cuts at: 0 8 236 1650
Size: 8 228 1414
Fragments arranged by size:
1414 228 8
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 809 1650
Size: 809 841
MscI TGG'CCA
Cuts at: 0 880 1132 1650
Size: 880 252 518
Fragments arranged by size:
880 518 252
MslI CAynn'nnrTG
Cuts at: 0 248 985 1567 1650
Size: 248 737 582 83
Fragments arranged by size:
737 582 248 83
MspA1I CmG'CkG
Cuts at: 0 295 771 847 1125 1351 1650
Size: 295 476 76 278 226 299
Fragments arranged by size:
476 299 295 278 226 76
NcoI C'CATG_G
Cuts at: 0 243 1650
Size: 243 1407
NspI r_CATG'y
Cuts at: 0 445 1650
Size: 445 1205
PflMI CCAn_nnn'nTGG
Cuts at: 0 1128 1650
Size: 1128 522
PsiI TTA'TAA
Cuts at: 0 794 1650
Size: 794 856
PspXI vC'TCGA_Gb
Cuts at: 0 1248 1650
Size: 1248 402
PvuII CAG'CTG
Cuts at: 0 295 771 1650
Size: 295 476 879
Fragments arranged by size:
879 476 295
RsrII CG'GwC_CG
Cuts at: 0 351 1650
Size: 351 1299
SapI GCTCTTCn'nnn_
Cuts at: 0 1624 1650
Size: 1624 26
SfcI C'TryA_G
Cuts at: 0 407 1296 1650
Size: 407 889 354
Fragments arranged by size:
889 407 354
SmaI CCC'GGG
Cuts at: 0 1427 1650
Size: 1427 223
SmlI C'TyrA_G
Cuts at: 0 205 1248 1650
Size: 205 1043 402
Fragments arranged by size:
1043 402 205
SphI G_CATG'C
Cuts at: 0 445 1650
Size: 445 1205
StyI C'CwwG_G
Cuts at: 0 243 741 1133 1650
Size: 243 498 392 517
Fragments arranged by size:
517 498 392 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 85 293 339 515 997 1650
Size: 85 208 46 176 482 653
Fragments arranged by size:
653 482 208 176 85 46
TatI w'GTAC_w
Cuts at: 0 1334 1650
Size: 1334 316
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 13 45 249 281 1650
Size: 13 32 204 32 1369
Fragments arranged by size:
1369 204 32 32 13
Tth111I GACn'n_nGTC
Cuts at: 0 349 1650
Size: 349 1301
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1167 1650
Size: 1167 483
XhoI C'TCGA_G
Cuts at: 0 1248 1650
Size: 1248 402
XmaI C'CCGG_G
Cuts at: 0 1425 1650
Size: 1425 225
Enzymes that do cut:
AarI AccI AflII AleI AlwNI ApoI AvaI BanI
BanII BbsI BbvCI BcgI BclI BglI BglII BlpI
Bme1580I BmrI BpmI Bpu10I BsaI BsaXI BseRI BseYI
BsgI BsiEI BsmI Bsp1286I BspMI BsrBI BsrFI BsrGI
BssSI BstXI BstYI BtgI BtgZI BtsI DraIII EaeI
EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII
HindIII MmeI MscI MslI MspA1I NcoI NspI PflMI
PsiI PspXI PvuII RsrII SapI SfcI SmaI SmlI
SphI StyI TaqII TatI TstI Tth111I XcmI XhoI
XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BciVI BfrBI BmgBI BmtI BplI
BpuEI BsaAI BsaBI BsaHI BsaWI BsiHKAI BsiWI BsmBI
BspEI BspHI BsrDI BssHII BstAPI BstBI BstEII BstZ17I
Bsu36I ClaI CspCI DraI DrdI EagI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HpaI KasI KpnI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PsrI PstI PvuI SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI
XbaI XmnI ZraI