(Circular) (Six-base) MAPSORT of: pFN21AB7015.seq Check: 1092 from: 1 to: 7123 pFN21AB7015 7123 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2278 2693 3026 3755 2278 Size: 415 333 729 5646 Fragments arranged by size: 5646 729 415 333 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4035 4271 4933 278 Size: 53 83 186 936 2499 236 662 2468 Fragments arranged by size: 2499 2468 936 662 236 186 83 53 AccI GT'mk_AC Cuts at: 1937 4014 4466 1937 Size: 2077 452 4594 Fragments arranged by size: 4594 2077 452 Acc65I G'GTAC_C Cuts at: 4444 4444 Size: 7123 AclI AA'CG_TT Cuts at: 1563 5250 5623 1563 Size: 3687 373 3063 Fragments arranged by size: 3687 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2350 2591 4145 5186 6570 6838 1632 Size: 718 241 1554 1041 1384 268 1917 Fragments arranged by size: 1917 1554 1384 1041 718 268 241 AfeI AGC'GCT Cuts at: 2747 2747 Size: 7123 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6926 AflIII A'CryG_T Cuts at: 1203 3288 6022 6999 1203 Size: 2085 2734 977 1327 Fragments arranged by size: 2734 2085 1327 977 AgeI A'CCGG_T Cuts at: 5954 5954 Size: 7123 AhdI GACnn_n'nnGTC Cuts at: 3571 3787 4036 5852 3571 Size: 216 249 1816 4842 Fragments arranged by size: 4842 1816 249 216 AleI CACnn'nnGTG Cuts at: 1166 2367 2438 4026 1166 Size: 1201 71 1588 4263 Fragments arranged by size: 4263 1588 1201 71 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2636 1184 Size: 1452 5671 AlwNI CAG_nnn'CTG Cuts at: 2284 2293 2540 4866 6438 2284 Size: 9 247 2326 1572 2969 Fragments arranged by size: 2969 2326 1572 247 9 ApaI G_GGCC'C Cuts at: 2126 2217 2277 2455 2903 3350 3415 3654 2126 Size: 91 60 178 448 447 65 239 5595 Fragments arranged by size: 5595 448 447 239 178 91 65 60 ApaLI G'TGCA_C Cuts at: 3236 3929 5180 6336 3236 Size: 693 1251 1156 4023 Fragments arranged by size: 4023 1251 1156 693 ApoI r'AATT_y Cuts at: 1493 4432 4658 4810 6919 1493 Size: 2939 226 152 2109 1697 Fragments arranged by size: 2939 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5677 160 Size: 872 4645 1606 Fragments arranged by size: 4645 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7123 AvaI C'yCGr_G Cuts at: 1870 1943 2119 2195 2395 2471 3374 3408 3721 Size: 73 176 76 200 76 903 34 313 Cuts at: 3721 3758 3810 3921 4318 4448 1870 Size: 37 52 111 397 130 4545 Fragments arranged by size: 4545 903 397 313 200 176 130 111 76 76 73 52 37 34 BamHI G'GATC_C Cuts at: 4453 4820 4453 Size: 367 6756 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2009 2060 2248 2659 3632 Size: 325 217 627 222 51 188 411 973 Cuts at: 3632 3988 4297 4444 5899 618 Size: 356 309 147 1455 1842 Fragments arranged by size: 1842 1455 973 627 411 356 325 309 222 217 188 147 51 BanII G_rGCy'C Cuts at: 1391 2126 2133 2217 2277 2455 2903 3227 3302 Size: 735 7 84 60 178 448 324 75 Cuts at: 3302 3350 3375 3415 3654 4082 4113 4415 4442 Size: 48 25 40 239 428 31 302 27 Cuts at: 4442 1391 Size: 4072 Fragments arranged by size: 4072 735 448 428 324 302 239 178 84 75 60 48 40 31 27 25 7 BbeI G_GCGC'C Cuts at: 2013 2064 3636 3992 4301 2013 Size: 51 1572 356 309 4835 Fragments arranged by size: 4835 1572 356 309 51 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2146 2415 3959 928 Size: 971 247 269 1544 4092 Fragments arranged by size: 4092 1544 971 269 247 BbvCI CC'TCA_GC Cuts at: 2427 3134 2427 Size: 707 6416 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3365 3399 5314 5348 1903 Size: 34 1428 34 1915 34 3678 Fragments arranged by size: 3678 1915 1428 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5015 6225 5015 Size: 1210 5913 BclI T'GATC_A Cuts at: 1548 4179 1548 Size: 2631 4492 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2054 2281 2504 Size: 107 122 71 631 605 382 227 223 Cuts at: 2504 2907 3027 5734 136 Size: 403 120 2707 1525 Fragments arranged by size: 2707 1525 631 605 403 382 227 223 122 120 107 71 BglII A'GATC_T Cuts at: 3243 4062 7118 3243 Size: 819 3056 3248 Fragments arranged by size: 3248 3056 819 BlpI GC'TnA_GC Cuts at: 2180 4540 2180 Size: 2360 4763 Bme1580I G_kGCm'C Cuts at: 1790 2126 2217 2253 2277 2455 2664 2903 3240 Size: 336 91 36 24 178 209 239 337 Cuts at: 3240 3350 3415 3654 3933 5184 6340 1790 Size: 110 65 239 279 1251 1156 2573 Fragments arranged by size: 2573 1251 1156 337 336 279 239 239 209 178 110 91 65 36 24 BmgBI CAC'GTC Cuts at: 1318 2737 2815 3928 1318 Size: 1419 78 1113 4513 Fragments arranged by size: 4513 1419 1113 78 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4854 5812 442 Size: 416 53 83 399 24 3437 958 1753 Fragments arranged by size: 3437 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7123 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2992 4330 2992 Size: 1338 5785 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2762 3299 4327 5783 1239 Size: 1523 537 1028 1456 2579 Fragments arranged by size: 2579 1523 1456 1028 537 Bpu10I CC'TnA_GC Cuts at: 2427 3134 2427 Size: 707 6416 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2852 2915 3245 5248 6113 6411 2852 Size: 63 330 2003 865 298 3564 Fragments arranged by size: 3564 2003 865 330 298 63 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5786 882 Size: 617 4287 2219 Fragments arranged by size: 4287 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2875 3661 4914 493 Size: 711 1671 786 1253 2702 Fragments arranged by size: 2702 1671 1253 786 711 BsaBI GATnn'nnATC Cuts at: 3899 7117 3899 Size: 3218 3905 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2010 2061 3003 3633 Size: 53 83 186 936 477 51 942 630 Cuts at: 3633 3989 4032 4268 4298 4930 5312 5947 275 Size: 356 43 236 30 632 382 635 1451 Fragments arranged by size: 1451 942 936 635 632 630 477 382 356 236 186 83 53 51 43 30 BsaWI w'CCGG_w Cuts at: 5556 5954 6228 6375 6786 5556 Size: 398 274 147 411 5893 Fragments arranged by size: 5893 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2297 2327 2371 2401 2687 Size: 30 165 30 894 30 44 30 286 Cuts at: 2687 2717 3230 3260 3815 3845 1178 Size: 30 513 30 555 30 4456 Fragments arranged by size: 4456 894 555 513 286 165 44 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2056 3098 3101 3776 4121 4339 1186 Size: 870 1042 3 675 345 218 3970 Fragments arranged by size: 3970 1042 870 675 345 218 3 BseYI C'CCAG_C Cuts at: 2277 2533 2803 3628 4072 4337 4400 6326 6822 Size: 256 270 825 444 265 63 1926 496 Cuts at: 6822 2277 Size: 2578 Fragments arranged by size: 2578 1926 825 496 444 270 265 256 63 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3622 4144 1163 Size: 573 1886 522 4142 Fragments arranged by size: 4142 1886 573 522 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2508 3967 4573 5334 6362 1540 Size: 275 186 507 1459 606 761 1028 2301 Fragments arranged by size: 2301 1459 1028 761 606 507 275 186 BsiHKAI G_wGCw'C Cuts at: 2601 2931 3240 3302 3375 3933 4113 5184 5269 Size: 330 309 62 73 558 180 1071 85 Cuts at: 5269 6340 2601 Size: 1071 3384 Fragments arranged by size: 3384 1071 1071 558 330 309 180 85 73 62 BsmI GAATG_Cn' Cuts at: 2302 4634 4727 2302 Size: 2332 93 4698 Fragments arranged by size: 4698 2332 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2126 2133 2217 2253 2277 2455 2601 Size: 399 336 7 84 36 24 178 146 Cuts at: 2601 2664 2903 2931 3227 3240 3302 3350 3375 Size: 63 239 28 296 13 62 48 25 Cuts at: 3375 3415 3654 3933 4082 4113 4415 4442 5184 Size: 40 239 279 149 31 302 27 742 Cuts at: 5184 5269 6340 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 336 302 296 279 239 239 178 149 146 85 84 63 62 48 40 36 31 28 27 25 24 13 7 BspEI T'CCGG_A Cuts at: 6786 6786 Size: 7123 BspHI T'CATG_A Cuts at: 5012 6742 5012 Size: 1730 5393 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2278 2693 3026 3633 3699 3755 4389 4478 Size: 1434 415 333 607 66 56 634 89 Cuts at: 4478 844 Size: 3489 Fragments arranged by size: 3489 1434 634 607 415 333 89 66 56 BsrBI CCG'CTC Cuts at: 1394 1823 2108 2476 3259 5010 1394 Size: 429 285 368 783 1751 3507 Fragments arranged by size: 3507 1751 783 429 368 285 BsrDI GCAATG_nn' Cuts at: 65 1248 3874 5618 5792 65 Size: 1183 2626 1744 174 1396 Fragments arranged by size: 2626 1744 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2057 2331 2503 2757 3966 5767 5954 Size: 110 247 274 172 254 1209 1801 187 Cuts at: 5954 1700 Size: 2869 Fragments arranged by size: 2869 1801 1209 274 254 247 187 172 110 BsrGI T'GTAC_A Cuts at: 96 2835 4173 96 Size: 2739 1338 3046 Fragments arranged by size: 3046 2739 1338 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7123 BssSI C'ACGA_G Cuts at: 2306 5183 6195 2306 Size: 2877 1012 3234 Fragments arranged by size: 3234 2877 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7123 BstBI TT'CG_AA Cuts at: 5927 5927 Size: 7123 BstEII G'GTnAC_C Cuts at: 1184 3686 3877 1184 Size: 2502 191 4430 Fragments arranged by size: 4430 2502 191 BstYI r'GATC_y Cuts at: 1972 3243 4062 4453 4820 5205 5222 6674 6760 Size: 1271 819 391 367 385 17 1452 86 Cuts at: 6760 6772 7118 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1271 819 391 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3337 3993 4351 3337 Size: 656 358 6109 Fragments arranged by size: 6109 656 358 BtgI C'CryG_G Cuts at: 513 1065 2004 2043 2208 2590 2671 513 Size: 552 939 39 165 382 81 4965 Fragments arranged by size: 4965 939 552 382 165 81 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3307 487 Size: 683 56 2081 4303 Fragments arranged by size: 4303 2081 683 56 BtsI GCAGTG_nn' Cuts at: 2639 2824 3121 4411 4639 5433 5453 2639 Size: 185 297 1290 228 794 20 4309 Fragments arranged by size: 4309 1290 794 297 228 185 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7123 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7088 DraI TTT'AAA Cuts at: 4427 4427 Size: 7123 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6130 809 Size: 507 4814 1802 Fragments arranged by size: 4814 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2046 2055 Size: 54 1425 50 275 18 210 6 9 Cuts at: 2055 2134 2496 2505 2784 3357 3454 3678 3964 Size: 79 362 9 279 573 97 224 286 Cuts at: 3964 4324 4570 5459 7088 8 Size: 360 246 889 1629 43 Fragments arranged by size: 1629 1425 889 573 362 360 286 279 275 246 224 210 97 79 54 50 43 18 9 9 6 EagI C'GGCC_G Cuts at: 1537 1812 2505 3964 4570 1537 Size: 275 693 1459 606 4090 Fragments arranged by size: 4090 1459 693 606 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4118 5053 1353 Size: 2765 935 3423 Fragments arranged by size: 3423 2765 935 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3485 5710 6084 6230 387 Size: 1064 138 1896 2225 374 146 1280 Fragments arranged by size: 2225 1896 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3300 3373 4111 3300 Size: 73 738 6312 Fragments arranged by size: 6312 738 73 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2350 2591 2762 3299 4145 4327 5186 Size: 393 718 241 171 537 846 182 859 Cuts at: 5186 5783 6570 6838 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 859 846 787 718 597 537 393 268 241 182 171 EcoNI CCTnn'n_nnAGG Cuts at: 3440 4213 3440 Size: 773 6350 EcoO109I rG'GnC_Cy Cuts at: 2213 2273 2274 2319 2451 2452 3346 3347 3556 Size: 60 1 45 132 1 894 1 209 Cuts at: 3556 3651 3781 3853 4048 4243 2213 Size: 95 130 72 195 195 5093 Fragments arranged by size: 5093 894 209 195 195 132 130 95 72 60 45 1 1 1 EcoRI G'AATT_C Cuts at: 4432 4810 4432 Size: 378 6745 FspI TGC'GCA Cuts at: 3200 3320 5629 3200 Size: 120 2309 4694 Fragments arranged by size: 4694 2309 120 FspAI rTGC'GCAy Cuts at: 3320 3320 Size: 7123 HaeII r_GCGC'y Cuts at: 1678 2013 2064 2749 2946 3636 3992 4301 6270 Size: 335 51 685 197 690 356 309 1969 Cuts at: 6270 1678 Size: 2531 Fragments arranged by size: 2531 1969 690 685 356 335 309 197 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2371 2375 2403 2407 3557 Size: 32 135 32 799 4 28 4 1150 Cuts at: 3557 3589 3773 3805 4022 4054 5764 5796 5838 Size: 32 184 32 217 32 1710 32 42 Cuts at: 5838 5870 1373 Size: 32 2626 Fragments arranged by size: 2626 1710 1150 799 217 184 135 42 32 32 32 32 32 32 32 28 4 4 HincII GTy'rAC Cuts at: 669 1657 1938 4467 4713 669 Size: 988 281 2529 246 3079 Fragments arranged by size: 3079 2529 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7123 HpaI GTT'AAC Cuts at: 4713 4713 Size: 7123 KasI G'GCGC_C Cuts at: 2009 2060 3632 3988 4297 2009 Size: 51 1572 356 309 4835 Fragments arranged by size: 4835 1572 356 309 51 KpnI G_GTAC'C Cuts at: 4448 4448 Size: 7123 MfeI C'AATT_G Cuts at: 4722 4722 Size: 7123 MluI A'CGCG_T Cuts at: 3288 6999 3288 Size: 3711 3412 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3309 3327 3543 6237 6421 1305 Size: 2004 18 216 2694 184 2007 Fragments arranged by size: 2694 2007 2004 216 184 18 MscI TGG'CCA Cuts at: 10 64 1489 1832 2048 3359 4326 10 Size: 54 1425 343 216 1311 967 2807 Fragments arranged by size: 2807 1425 1311 967 343 216 54 MslI CAynn'nnrTG Cuts at: 518 1166 2367 2438 3167 4026 4100 4181 5081 Size: 648 1201 71 729 859 74 81 900 Cuts at: 5081 5440 5599 518 Size: 359 159 2042 Fragments arranged by size: 2042 1201 900 859 729 648 359 159 81 74 71 MspA1I CmG'CkG Cuts at: 1606 2163 2281 2328 2338 2529 2540 2592 2673 Size: 557 118 47 10 191 11 52 81 Cuts at: 2673 4234 4539 5216 6364 6609 1606 Size: 1561 305 677 1148 245 2120 Fragments arranged by size: 2120 1561 1148 677 557 305 245 191 118 81 52 47 11 10 NaeI GCC'GGC Cuts at: 2059 2333 2505 3968 2059 Size: 274 172 1463 5214 Fragments arranged by size: 5214 1463 274 172 NarI GG'CG_CC Cuts at: 2010 2061 3633 3989 4298 2010 Size: 51 1572 356 309 4835 Fragments arranged by size: 4835 1572 356 309 51 NcoI C'CATG_G Cuts at: 513 1065 2004 2208 513 Size: 552 939 204 5428 Fragments arranged by size: 5428 939 552 204 NdeI CA'TA_TG Cuts at: 387 387 Size: 7123 NgoMIV G'CCGG_C Cuts at: 2057 2331 2503 3966 2057 Size: 274 172 1463 5214 Fragments arranged by size: 5214 1463 274 172 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7123 NotI GC'GGCC_GC Cuts at: 4570 4570 Size: 7123 NspI r_CATG'y Cuts at: 1132 1229 4202 4481 6026 1132 Size: 97 2973 279 1545 2229 Fragments arranged by size: 2973 2229 1545 279 97 PasI CC'CwG_GG Cuts at: 1348 3424 1348 Size: 2076 5047 PciI A'CATG_T Cuts at: 6022 6022 Size: 7123 PflMI CCAn_nnn'nTGG Cuts at: 1108 2223 2540 2565 4141 1108 Size: 1115 317 25 1576 4090 Fragments arranged by size: 4090 1576 1115 317 25 PmeI GTTT'AAAC Cuts at: 4427 4427 Size: 7123 PmlI CAC'GTG Cuts at: 2875 2875 Size: 7123 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5192 5224 6705 6737 5192 Size: 32 1481 32 5578 Fragments arranged by size: 5578 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3556 3781 3853 4048 4243 3556 Size: 225 72 195 195 6436 Fragments arranged by size: 6436 225 195 195 72 PshAI GACnn'nnGTC Cuts at: 1613 3271 1613 Size: 1658 5465 PsiI TTA'TAA Cuts at: 4693 4693 Size: 7123 PspOMI G'GGCC_C Cuts at: 2122 2213 2273 2451 2899 3346 3411 3650 2122 Size: 91 60 178 448 447 65 239 5595 Fragments arranged by size: 5595 448 447 239 178 91 65 60 PspXI vC'TCGA_Gb Cuts at: 3374 3374 Size: 7123 PstI C_TGCA'G Cuts at: 830 2289 2772 4475 830 Size: 1459 483 1703 3478 Fragments arranged by size: 3478 1703 1459 483 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7123 PvuII CAG'CTG Cuts at: 2328 4234 2328 Size: 1906 5217 RsrII CG'GwC_CG Cuts at: 2668 2668 Size: 7123 SacI G_AGCT'C Cuts at: 3302 3375 4113 3302 Size: 73 738 6312 Fragments arranged by size: 6312 738 73 SacII CC_GC'GG Cuts at: 2593 2674 2593 Size: 81 7042 SalI G'TCGA_C Cuts at: 1936 4465 1936 Size: 2529 4594 SapI GCTCTTCn'nnn_ Cuts at: 4118 4118 Size: 7123 SbfI CC_TGCA'GG Cuts at: 2772 4475 2772 Size: 1703 5420 ScaI AGT'ACT Cuts at: 3824 4313 5371 7083 3824 Size: 489 1058 1712 3864 Fragments arranged by size: 3864 1712 1058 489 SexAI A'CCwGG_T Cuts at: 3567 3783 3801 3567 Size: 216 18 6889 Fragments arranged by size: 6889 216 18 SfcI C'TryA_G Cuts at: 826 1045 2285 2323 2768 4471 5606 6287 6478 Size: 219 1240 38 445 1703 1135 681 191 Cuts at: 6478 826 Size: 1471 Fragments arranged by size: 1703 1471 1240 1135 681 445 219 191 38 SfiI GGCCn_nnn'nGGCC Cuts at: 2054 2504 2054 Size: 450 6673 SfoI GGC'GCC Cuts at: 2011 2062 3634 3990 4299 2011 Size: 51 1572 356 309 4835 Fragments arranged by size: 4835 1572 356 309 51 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7123 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7123 SmaI CCC'GGG Cuts at: 1872 2121 2197 3723 3812 3923 4320 4450 1872 Size: 249 76 1526 89 111 397 130 4545 Fragments arranged by size: 4545 1526 397 249 130 111 89 76 SmlI C'TyrA_G Cuts at: 820 1017 1943 2867 2930 3260 3374 5227 6128 Size: 197 926 924 63 330 114 1853 901 Cuts at: 6128 6390 820 Size: 262 1553 Fragments arranged by size: 1853 1553 926 924 901 330 262 197 114 63 SnaBI TAC'GTA Cuts at: 493 4914 493 Size: 4421 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7123 SphI G_CATG'C Cuts at: 1132 4202 4481 1132 Size: 3070 279 3774 Fragments arranged by size: 3774 3070 279 SspI AAT'ATT Cuts at: 5 52 5047 5 Size: 47 4995 2081 Fragments arranged by size: 4995 2081 47 StuI AGG'CCT Cuts at: 1517 3219 3998 1517 Size: 1702 779 4642 Fragments arranged by size: 4642 1702 779 StyI C'CwwG_G Cuts at: 513 1065 2004 2076 2208 3436 3681 4562 513 Size: 552 939 72 132 1228 245 881 3074 Fragments arranged by size: 3074 1228 939 881 552 245 132 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3896 4023 4414 5150 5167 5320 5505 Size: 1255 2316 127 391 736 17 153 185 Cuts at: 5505 5950 325 Size: 445 1498 Fragments arranged by size: 2316 1498 1255 736 445 391 185 153 127 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2835 3012 3822 Size: 274 80 33 51 1443 858 177 810 Cuts at: 3822 4173 4311 5369 7081 96 Size: 351 138 1058 1712 138 Fragments arranged by size: 1712 1443 1058 858 810 351 274 177 138 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2297 2329 3230 3262 7083 7115 2297 Size: 32 901 32 3821 32 2305 Fragments arranged by size: 3821 2305 901 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4010 4253 1316 Size: 2694 243 4186 Fragments arranged by size: 4186 2694 243 XbaI T'CTAG_A Cuts at: 4459 4459 Size: 7123 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3217 3310 3217 Size: 93 7030 XhoI C'TCGA_G Cuts at: 1943 3374 1943 Size: 1431 5692 XmaI C'CCGG_G Cuts at: 1870 2119 2195 3721 3810 3921 4318 4448 1870 Size: 249 76 1526 89 111 397 130 4545 Fragments arranged by size: 4545 1526 397 249 130 111 89 76 XmnI GAAnn'nnTTC Cuts at: 5252 5252 Size: 7123 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4033 4269 4931 276 Size: 53 83 186 936 2499 236 662 2468 Fragments arranged by size: 2499 2468 936 662 236 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI NruI NsiI PacI PfoI PsrI SanDI SrfI SwaI