Restriction map for pFN21AB7015
 (Linear) (Six-base) MAPSORT of: KIBB7015.seq  Check: 9483  from: 1  to: 2418



KIBB7015.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     273     688    1021    1750    2418 
   Size:        273     415     333     729     668

  Fragments arranged by size: 

                 729     668     415     333     273
 
AatII G_ACGT'C

Cuts at:      0    2030    2266    2418 
   Size:       2030     236     152

  Fragments arranged by size: 

                2030     236     152
 
AccI GT'mk_AC

Cuts at:      0    2009    2418 
   Size:       2009     409
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     345     586    2140    2418 
   Size:        345     241    1554     278

  Fragments arranged by size: 

                1554     345     278     241
 
AfeI AGC'GCT

Cuts at:      0     742    2418 
   Size:        742    1676
 
AflIII A'CryG_T

Cuts at:      0    1283    2418 
   Size:       1283    1135
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1566    1782    2031    2418 
   Size:       1566     216     249     387

  Fragments arranged by size: 

                1566     387     249     216
 
AleI CACnn'nnGTG

Cuts at:      0     362     433    2021    2418 
   Size:        362      71    1588     397

  Fragments arranged by size: 

                1588     397     362      71
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     631    2418 
   Size:        631    1787
 
AlwNI CAG_nnn'CTG

Cuts at:      0     279     288     535    2418 
   Size:        279       9     247    1883

  Fragments arranged by size: 

                1883     279     247       9
 
ApaI G_GGCC'C

Cuts at:      0     121     212     272     450     898    1345    1410    1649 
   Size:        121      91      60     178     448     447      65     239

Cuts at:   1649    2418 
   Size:        769

  Fragments arranged by size: 

                 769     448     447     239     178     121      91      65
                  60
 
ApaLI G'TGCA_C

Cuts at:      0    1231    1924    2418 
   Size:       1231     693     494

  Fragments arranged by size: 

                1231     693     494
 
AvaI C'yCGr_G

Cuts at:      0     114     190     390     466    1369    1403    1716    1753 
   Size:        114      76     200      76     903      34     313      37

Cuts at:   1753    1805    1916    2313    2418 
   Size:         52     111     397     105

  Fragments arranged by size: 

                 903     397     313     200     114     111     105      76
                  76      52      37      34
 
BanI G'GyrC_C

Cuts at:      0       4      55     243     654    1627    1983    2292    2418 
   Size:          4      51     188     411     973     356     309     126

  Fragments arranged by size: 

                 973     411     356     309     188     126      51       4
            
 
BanII G_rGCy'C

Cuts at:      0     121     128     212     272     450     898    1222    1297 
   Size:        121       7      84      60     178     448     324      75

Cuts at:   1297    1345    1370    1410    1649    2077    2108    2410    2418 
   Size:         48      25      40     239     428      31     302       8

  Fragments arranged by size: 

                 448     428     324     302     239     178     121      84
                  75      60      48      40      31      25       8       7
            
 
BbeI G_GCGC'C

Cuts at:      0       8      59    1631    1987    2296    2418 
   Size:          8      51    1572     356     309     122

  Fragments arranged by size: 

                1572     356     309     122      51       8
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     141     410    1954    2418 
   Size:        141     269    1544     464

  Fragments arranged by size: 

                1544     464     269     141
 
BbvCI CC'TCA_GC

Cuts at:      0     422    1129    2418 
   Size:        422     707    1289

  Fragments arranged by size: 

                1289     707     422
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1360    1394    2418 
   Size:       1360      34    1024

  Fragments arranged by size: 

                1360    1024      34
 
BclI T'GATC_A

Cuts at:      0    2174    2418 
   Size:       2174     244
 
BglI GCCn_nnn'nGGC

Cuts at:      0      49     276     499     902    1022    2418 
   Size:         49     227     223     403     120    1396

  Fragments arranged by size: 

                1396     403     227     223     120      49
 
BglII A'GATC_T

Cuts at:      0    1238    2057    2418 
   Size:       1238     819     361

  Fragments arranged by size: 

                1238     819     361
 
BlpI GC'TnA_GC

Cuts at:      0     175    2418 
   Size:        175    2243
 
Bme1580I G_kGCm'C

Cuts at:      0     121     212     248     272     450     659     898    1235 
   Size:        121      91      36      24     178     209     239     337

Cuts at:   1235    1345    1410    1649    1928    2418 
   Size:        110      65     239     279     490

  Fragments arranged by size: 

                 490     337     279     239     239     209     178     121
                 110      91      65      36      24
 
BmgBI CAC'GTC

Cuts at:      0     732     810    1923    2418 
   Size:        732      78    1113     495

  Fragments arranged by size: 

                1113     732     495      78
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     987    2325    2418 
   Size:        987    1338      93

  Fragments arranged by size: 

                1338     987      93
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     757    1294    2322    2418 
   Size:        757     537    1028      96

  Fragments arranged by size: 

                1028     757     537      96
 
Bpu10I CC'TnA_GC

Cuts at:      0     422    1129    2418 
   Size:        422     707    1289

  Fragments arranged by size: 

                1289     707     422
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     847     910    1240    2418 
   Size:        847      63     330    1178

  Fragments arranged by size: 

                1178     847     330      63
 
BsaAI yAC'GTr

Cuts at:      0     870    1656    2418 
   Size:        870     786     762

  Fragments arranged by size: 

                 870     786     762
 
BsaBI GATnn'nnATC

Cuts at:      0    1894    2418 
   Size:       1894     524
 
BsaHI Gr'CG_yC

Cuts at:      0       5      56     998    1628    1984    2027    2263    2293 
   Size:          5      51     942     630     356      43     236      30

Cuts at:   2293    2418 
   Size:        125

  Fragments arranged by size: 

                 942     630     356     236     125      51      43      30
                   5
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     292     322     366     396     682     712    1225    1255 
   Size:        292      30      44      30     286      30     513      30

Cuts at:   1255    1810    1840    2418 
   Size:        555      30     578

  Fragments arranged by size: 

                 578     555     513     292     286      44      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      51    1093    1096    1771    2116    2334    2418 
   Size:         51    1042       3     675     345     218      84

  Fragments arranged by size: 

                1042     675     345     218      84      51       3
 
BseYI C'CCAG_C

Cuts at:      0     272     528     798    1623    2067    2332    2395    2418 
   Size:        272     256     270     825     444     265      63      23

  Fragments arranged by size: 

                 825     444     272     270     265     256      63      23
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1617    2139    2418 
   Size:       1617     522     279

  Fragments arranged by size: 

                1617     522     279
 
BsiEI CG_ry'CG

Cuts at:      0     503    1962    2418 
   Size:        503    1459     456

  Fragments arranged by size: 

                1459     503     456
 
BsiHKAI G_wGCw'C

Cuts at:      0     596     926    1235    1297    1370    1928    2108    2418 
   Size:        596     330     309      62      73     558     180     310

  Fragments arranged by size: 

                 596     558     330     310     309     180      73      62
            
 
BsmI GAATG_Cn'

Cuts at:      0     297    2418 
   Size:        297    2121
 
Bsp1286I G_dGCh'C

Cuts at:      0     121     128     212     248     272     450     596     659 
   Size:        121       7      84      36      24     178     146      63

Cuts at:    659     898     926    1222    1235    1297    1345    1370    1410 
   Size:        239      28     296      13      62      48      25      40

Cuts at:   1410    1649    1928    2077    2108    2410    2418 
   Size:        239     279     149      31     302       8

  Fragments arranged by size: 

                 302     296     279     239     239     178     149     146
                 121      84      63      62      48      40      36      31
                  28      25      24      13       8       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     273     688    1021    1628    1694    1750    2384    2418 
   Size:        273     415     333     607      66      56     634      34

  Fragments arranged by size: 

                 634     607     415     333     273      66      56      34
            
 
BsrBI CCG'CTC

Cuts at:      0     103     471    1254    2418 
   Size:        103     368     783    1164

  Fragments arranged by size: 

                1164     783     368     103
 
BsrDI GCAATG_nn'

Cuts at:      0    1869    2418 
   Size:       1869     549
 
BsrFI r'CCGG_y

Cuts at:      0      52     326     498     752    1961    2418 
   Size:         52     274     172     254    1209     457

  Fragments arranged by size: 

                1209     457     274     254     172      52
 
BsrGI T'GTAC_A

Cuts at:      0     830    2168    2418 
   Size:        830    1338     250

  Fragments arranged by size: 

                1338     830     250
 
BssSI C'ACGA_G

Cuts at:      0     301    2418 
   Size:        301    2117
 
BstEII G'GTnAC_C

Cuts at:      0    1681    1872    2418 
   Size:       1681     191     546

  Fragments arranged by size: 

                1681     546     191
 
BstYI r'GATC_y

Cuts at:      0    1238    2057    2418 
   Size:       1238     819     361

  Fragments arranged by size: 

                1238     819     361
 
Bsu36I CC'TnA_GG

Cuts at:      0    1332    1988    2346    2418 
   Size:       1332     656     358      72

  Fragments arranged by size: 

                1332     656     358      72
 
BtgI C'CryG_G

Cuts at:      0      38     203     585     666    2418 
   Size:         38     165     382      81    1752

  Fragments arranged by size: 

                1752     382     165      81      38
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1302    2418 
   Size:       1302    1116
 
BtsI GCAGTG_nn'

Cuts at:      0     634     819    1116    2406    2418 
   Size:        634     185     297    1290      12

  Fragments arranged by size: 

                1290     634     297     185      12
 
EaeI y'GGCC_r

Cuts at:      0      35      41      50     129     491     500     779    1352 
   Size:         35       6       9      79     362       9     279     573

Cuts at:   1352    1449    1673    1959    2319    2418 
   Size:         97     224     286     360      99

  Fragments arranged by size: 

                 573     362     360     286     279     224      99      97
                  79      35       9       9       6
 
EagI C'GGCC_G

Cuts at:      0     500    1959    2418 
   Size:        500    1459     459

  Fragments arranged by size: 

                1459     500     459
 
EarI CTCTTCn'nnn_

Cuts at:      0    2113    2418 
   Size:       2113     305
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1480    2418 
   Size:       1480     938
 
EcoICRI GAG'CTC

Cuts at:      0    1295    1368    2106    2418 
   Size:       1295      73     738     312

  Fragments arranged by size: 

                1295     738     312      73
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     345     586     757    1294    2140    2322    2418 
   Size:        345     241     171     537     846     182      96

  Fragments arranged by size: 

                 846     537     345     241     182     171      96
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1435    2208    2418 
   Size:       1435     773     210

  Fragments arranged by size: 

                1435     773     210
 
EcoO109I rG'GnC_Cy

Cuts at:      0     208     268     269     314     446     447    1341    1342 
   Size:        208      60       1      45     132       1     894       1

Cuts at:   1342    1551    1646    1776    1848    2043    2238    2418 
   Size:        209      95     130      72     195     195     180

  Fragments arranged by size: 

                 894     209     208     195     195     180     132     130
                  95      72      60      45       1       1       1
 
FspI TGC'GCA

Cuts at:      0    1195    1315    2418 
   Size:       1195     120    1103

  Fragments arranged by size: 

                1195    1103     120
 
FspAI rTGC'GCAy

Cuts at:      0    1315    2418 
   Size:       1315    1103
 
HaeII r_GCGC'y

Cuts at:      0       8      59     744     941    1631    1987    2296    2418 
   Size:          8      51     685     197     690     356     309     122

  Fragments arranged by size: 

                 690     685     356     309     197     122      51       8
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     366     370     398     402    1552    1584    1768    1800 
   Size:        366       4      28       4    1150      32     184      32

Cuts at:   1800    2017    2049    2418 
   Size:        217      32     369

  Fragments arranged by size: 

                1150     369     366     217     184      32      32      32
                  28       4       4
 
KasI G'GCGC_C

Cuts at:      0       4      55    1627    1983    2292    2418 
   Size:          4      51    1572     356     309     126

  Fragments arranged by size: 

                1572     356     309     126      51       4
 
MluI A'CGCG_T

Cuts at:      0    1283    2418 
   Size:       1283    1135
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1304    1322    1538    2418 
   Size:       1304      18     216     880

  Fragments arranged by size: 

                1304     880     216      18
 
MscI TGG'CCA

Cuts at:      0      43    1354    2321    2418 
   Size:         43    1311     967      97

  Fragments arranged by size: 

                1311     967      97      43
 
MslI CAynn'nnrTG

Cuts at:      0     362     433    1162    2021    2095    2176    2418 
   Size:        362      71     729     859      74      81     242

  Fragments arranged by size: 

                 859     729     362     242      81      74      71
 
MspA1I CmG'CkG

Cuts at:      0     158     276     323     333     524     535     587     668 
   Size:        158     118      47      10     191      11      52      81

Cuts at:    668    2229    2418 
   Size:       1561     189

  Fragments arranged by size: 

                1561     191     189     158     118      81      52      47
                  11      10
 
NaeI GCC'GGC

Cuts at:      0      54     328     500    1963    2418 
   Size:         54     274     172    1463     455

  Fragments arranged by size: 

                1463     455     274     172      54
 
NarI GG'CG_CC

Cuts at:      0       5      56    1628    1984    2293    2418 
   Size:          5      51    1572     356     309     125

  Fragments arranged by size: 

                1572     356     309     125      51       5
 
NcoI C'CATG_G

Cuts at:      0     203    2418 
   Size:        203    2215
 
NgoMIV G'CCGG_C

Cuts at:      0      52     326     498    1961    2418 
   Size:         52     274     172    1463     457

  Fragments arranged by size: 

                1463     457     274     172      52
 
NspI r_CATG'y

Cuts at:      0    2197    2418 
   Size:       2197     221
 
PasI CC'CwG_GG

Cuts at:      0    1419    2418 
   Size:       1419     999
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     218     535     560    2136    2418 
   Size:        218     317      25    1576     282

  Fragments arranged by size: 

                1576     317     282     218      25
 
PmlI CAC'GTG

Cuts at:      0     870    2418 
   Size:        870    1548
 
PpuMI rG'GwC_Cy

Cuts at:      0    1551    1776    1848    2043    2238    2418 
   Size:       1551     225      72     195     195     180

  Fragments arranged by size: 

                1551     225     195     195     180      72
 
PshAI GACnn'nnGTC

Cuts at:      0    1266    2418 
   Size:       1266    1152
 
PspOMI G'GGCC_C

Cuts at:      0     117     208     268     446     894    1341    1406    1645 
   Size:        117      91      60     178     448     447      65     239

Cuts at:   1645    2418 
   Size:        773

  Fragments arranged by size: 

                 773     448     447     239     178     117      91      65
                  60
 
PspXI vC'TCGA_Gb

Cuts at:      0    1369    2418 
   Size:       1369    1049
 
PstI C_TGCA'G

Cuts at:      0     284     767    2418 
   Size:        284     483    1651

  Fragments arranged by size: 

                1651     483     284
 
PvuII CAG'CTG

Cuts at:      0     323    2229    2418 
   Size:        323    1906     189

  Fragments arranged by size: 

                1906     323     189
 
RsrII CG'GwC_CG

Cuts at:      0     663    2418 
   Size:        663    1755
 
SacI G_AGCT'C

Cuts at:      0    1297    1370    2108    2418 
   Size:       1297      73     738     310

  Fragments arranged by size: 

                1297     738     310      73
 
SacII CC_GC'GG

Cuts at:      0     588     669    2418 
   Size:        588      81    1749

  Fragments arranged by size: 

                1749     588      81
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2113    2418 
   Size:       2113     305
 
SbfI CC_TGCA'GG

Cuts at:      0     767    2418 
   Size:        767    1651
 
ScaI AGT'ACT

Cuts at:      0    1819    2308    2418 
   Size:       1819     489     110

  Fragments arranged by size: 

                1819     489     110
 
SexAI A'CCwGG_T

Cuts at:      0    1562    1778    1796    2418 
   Size:       1562     216      18     622

  Fragments arranged by size: 

                1562     622     216      18
 
SfcI C'TryA_G

Cuts at:      0     280     318     763    2418 
   Size:        280      38     445    1655

  Fragments arranged by size: 

                1655     445     280      38
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      49     499    2418 
   Size:         49     450    1919

  Fragments arranged by size: 

                1919     450      49
 
SfoI GGC'GCC

Cuts at:      0       6      57    1629    1985    2294    2418 
   Size:          6      51    1572     356     309     124

  Fragments arranged by size: 

                1572     356     309     124      51       6
 
SmaI CCC'GGG

Cuts at:      0     116     192    1718    1807    1918    2315    2418 
   Size:        116      76    1526      89     111     397     103

  Fragments arranged by size: 

                1526     397     116     111     103      89      76
 
SmlI C'TyrA_G

Cuts at:      0     862     925    1255    1369    2418 
   Size:        862      63     330     114    1049

  Fragments arranged by size: 

                1049     862     330     114      63
 
SphI G_CATG'C

Cuts at:      0    2197    2418 
   Size:       2197     221
 
StuI AGG'CCT

Cuts at:      0    1214    1993    2418 
   Size:       1214     779     425

  Fragments arranged by size: 

                1214     779     425
 
StyI C'CwwG_G

Cuts at:      0      71     203    1431    1676    2418 
   Size:         71     132    1228     245     742

  Fragments arranged by size: 

                1228     742     245     132      71
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1891    2018    2409    2418 
   Size:       1891     127     391       9

  Fragments arranged by size: 

                1891     391     127       9
 
TatI w'GTAC_w

Cuts at:      0     830    1007    1817    2168    2306    2418 
   Size:        830     177     810     351     138     112

  Fragments arranged by size: 

                 830     810     351     177     138     112
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     292     324    1225    1257    2418 
   Size:        292      32     901      32    1161

  Fragments arranged by size: 

                1161     901     292      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2005    2248    2418 
   Size:       2005     243     170

  Fragments arranged by size: 

                2005     243     170
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1212    1305    2418 
   Size:       1212      93    1113

  Fragments arranged by size: 

                1212    1113      93
 
XhoI C'TCGA_G

Cuts at:      0    1369    2418 
   Size:       1369    1049
 
XmaI C'CCGG_G

Cuts at:      0     114     190    1716    1805    1916    2313    2418 
   Size:        114      76    1526      89     111     397     105

  Fragments arranged by size: 

                1526     397     114     111     105      89      76
 
ZraI GAC'GTC

Cuts at:      0    2028    2264    2418 
   Size:       2028     236     154

  Fragments arranged by size: 

                2028     236     154
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI   AflIII     AhdI     AleI
     AlfI    AlwNI     ApaI    ApaLI     AvaI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstEII    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I     FspI    FspAI    HaeII    Hin4I
     KasI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI     PasI    PflMI     PmlI    PpuMI    PshAI
   PspOMI    PspXI     PstI    PvuII    RsrII     SacI    SacII     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SmaI     SmlI
     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI
     XhoI     XmaI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII     AgeI     AloI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BciVI    BfrBI     BmrI     BmtI
     BsaI    BsaWI    BsiWI    BsmBI    BspEI    BspHI   BssHII   BstAPI
    BstBI    BstXI  BstZ17I     ClaI    CspCI     DraI   DraIII     DrdI
    EcoRI    EcoRV     FalI     FseI   HincII  HindIII     HpaI     KpnI
     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI     PciI
     PfoI     PmeI     PpiI     PsiI     PsrI     PvuI     SalI    SanDI
     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     SwaI     XbaI
     XmnI
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