(Circular) (Six-base) MAPSORT of: pFN21AB5550.seq Check: 6861 from: 1 to: 6667 pFN21AB5550 6667 bp With 182 enzymes: SgfI * October 20, 2009 10:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4477 278 Size: 53 83 186 936 2941 2468 Fragments arranged by size: 2941 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3014 4010 1937 Size: 1077 996 4594 Fragments arranged by size: 4594 1077 996 Acc65I G'GTAC_C Cuts at: 3988 3988 Size: 6667 AclI AA'CG_TT Cuts at: 1563 4794 5167 1563 Size: 3231 373 3063 Fragments arranged by size: 3231 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2235 2451 3564 3630 3789 4730 6114 6382 Size: 603 216 1113 66 159 941 1384 268 Cuts at: 6382 1632 Size: 1917 Fragments arranged by size: 1917 1384 1113 941 603 268 216 159 66 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6470 AflIII A'CryG_T Cuts at: 1203 5566 6543 1203 Size: 4363 977 1327 Fragments arranged by size: 4363 1327 977 AgeI A'CCGG_T Cuts at: 5498 5498 Size: 6667 AhdI GACnn_n'nnGTC Cuts at: 5396 5396 Size: 6667 AleI CACnn'nnGTG Cuts at: 1166 3329 1166 Size: 2163 4504 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6667 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2327 2359 2327 Size: 32 6635 AlwNI CAG_nnn'CTG Cuts at: 3124 3368 4410 5982 3124 Size: 244 1042 1572 3809 Fragments arranged by size: 3809 1572 1042 244 ApaI G_GGCC'C Cuts at: 2899 2899 Size: 6667 ApaLI G'TGCA_C Cuts at: 4724 5880 4724 Size: 1156 5511 ApoI r'AATT_y Cuts at: 1493 2436 2981 3105 3462 3976 4202 4354 6463 Size: 943 545 124 357 514 226 152 2109 Cuts at: 6463 1493 Size: 1697 Fragments arranged by size: 2109 1697 943 545 514 357 226 152 124 AseI AT'TA_AT Cuts at: 160 1032 5221 160 Size: 872 4189 1606 Fragments arranged by size: 4189 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6667 AvaI C'yCGr_G Cuts at: 1870 1943 2267 2973 3992 1870 Size: 73 324 706 1019 4545 Fragments arranged by size: 4545 1019 706 324 73 BamHI G'GATC_C Cuts at: 3997 4364 3997 Size: 367 6300 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3331 3988 5443 618 Size: 325 217 627 1544 657 1455 1842 Fragments arranged by size: 1842 1544 1455 657 627 325 217 BanII G_rGCy'C Cuts at: 1391 2899 2994 3986 1391 Size: 1508 95 992 4072 Fragments arranged by size: 4072 1508 992 95 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2719 2778 2828 2895 2958 3552 3663 Size: 971 820 59 50 67 63 594 111 Cuts at: 3663 3852 928 Size: 189 3743 Fragments arranged by size: 3743 971 820 594 189 111 67 63 59 50 BbvCI CC'TCA_GC Cuts at: 2475 3651 2475 Size: 1176 5491 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2017 2051 2077 2111 4858 4892 1903 Size: 34 80 34 26 34 2747 34 3678 Fragments arranged by size: 3678 2747 80 34 34 34 34 26 BciVI GTATCCnnnnn_n' Cuts at: 3937 4559 5769 3937 Size: 622 1210 4835 Fragments arranged by size: 4835 1210 622 BclI T'GATC_A Cuts at: 1548 3226 1548 Size: 1678 4989 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2894 5278 136 Size: 107 122 71 631 605 1222 2384 1525 Fragments arranged by size: 2384 1525 1222 631 605 122 107 71 BglII A'GATC_T Cuts at: 3351 6662 3351 Size: 3311 3356 BlpI GC'TnA_GC Cuts at: 2042 4084 2042 Size: 2042 4625 Bme1580I G_kGCm'C Cuts at: 1790 2090 2899 4728 5884 1790 Size: 300 809 1829 1156 2573 Fragments arranged by size: 2573 1829 1156 809 300 BmgBI CAC'GTC Cuts at: 1318 2083 1318 Size: 765 5902 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4398 5356 442 Size: 416 53 83 399 24 2981 958 1753 Fragments arranged by size: 2981 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6667 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5327 1239 Size: 4088 2579 Bpu10I CC'TnA_GC Cuts at: 2475 3651 2475 Size: 1176 5491 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3653 4792 5657 5955 3653 Size: 1139 865 298 4365 Fragments arranged by size: 4365 1139 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2957 3773 5330 882 Size: 617 1458 816 1557 2219 Fragments arranged by size: 2219 1557 1458 816 617 BsaAI yAC'GTr Cuts at: 493 1204 2310 4458 493 Size: 711 1106 2148 2702 Fragments arranged by size: 2702 2148 1106 711 BsaBI GATnn'nnATC Cuts at: 2757 3578 6661 2757 Size: 821 3083 2763 Fragments arranged by size: 3083 2763 821 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2286 4474 4856 5491 Size: 53 83 186 936 753 2188 382 635 Cuts at: 5491 275 Size: 1451 Fragments arranged by size: 2188 1451 936 753 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3009 5100 5498 5772 5919 6330 3009 Size: 2091 398 274 147 411 3346 Fragments arranged by size: 3346 2091 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2410 2440 1178 Size: 30 165 30 1007 30 5405 Fragments arranged by size: 5405 1007 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2075 2078 3465 1186 Size: 889 3 1387 4388 Fragments arranged by size: 4388 1387 889 3 BseYI C'CCAG_C Cuts at: 3748 5870 6366 3748 Size: 2122 496 4049 Fragments arranged by size: 4049 2122 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2348 2879 3936 1163 Size: 573 612 531 1057 3894 Fragments arranged by size: 3894 1057 612 573 531 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2149 2305 4117 4878 5906 1540 Size: 275 186 148 156 1812 761 1028 2301 Fragments arranged by size: 2301 1812 1028 761 275 186 156 148 BsiHKAI G_wGCw'C Cuts at: 2994 3219 4728 4813 5884 2994 Size: 225 1509 85 1071 3777 Fragments arranged by size: 3777 1509 1071 225 85 BsmI GAATG_Cn' Cuts at: 4178 4271 4178 Size: 93 6574 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2090 2899 2994 3219 3986 4728 4813 Size: 399 300 809 95 225 767 742 85 Cuts at: 4813 5884 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 809 767 742 399 300 225 95 85 BspEI T'CCGG_A Cuts at: 6330 6330 Size: 6667 BspHI T'CATG_A Cuts at: 3300 4556 6286 3300 Size: 1256 1730 3681 Fragments arranged by size: 3681 1730 1256 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4022 844 Size: 3178 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4554 1394 Size: 429 2731 3507 Fragments arranged by size: 3507 2731 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5162 5336 65 Size: 1183 3914 174 1396 Fragments arranged by size: 3914 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5311 5498 1700 Size: 110 3501 187 2869 Fragments arranged by size: 3501 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2811 96 Size: 2715 3952 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6667 BssSI C'ACGA_G Cuts at: 3477 4727 5739 3477 Size: 1250 1012 4405 Fragments arranged by size: 4405 1250 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6667 BstBI TT'CG_AA Cuts at: 2730 5471 2730 Size: 2741 3926 BstEII G'GTnAC_C Cuts at: 1184 2497 3239 1184 Size: 1313 742 4612 Fragments arranged by size: 4612 1313 742 BstYI r'GATC_y Cuts at: 1972 3001 3351 3997 4364 4749 4766 6218 6304 Size: 1029 350 646 367 385 17 1452 86 Cuts at: 6304 6316 6662 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1029 646 385 367 350 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2445 3160 3783 513 Size: 552 1380 715 623 3397 Fragments arranged by size: 3397 1380 715 623 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2042 2146 2250 487 Size: 683 56 816 104 104 4904 Fragments arranged by size: 4904 816 683 104 104 56 BtsI GCAGTG_nn' Cuts at: 4183 4977 4997 4183 Size: 794 20 5853 Fragments arranged by size: 5853 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6667 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6632 DraI TTT'AAA Cuts at: 3971 3971 Size: 6667 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2723 2786 5674 809 Size: 507 1407 63 2888 1802 Fragments arranged by size: 2888 1802 1407 507 63 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 2146 2302 Size: 54 1425 50 275 18 192 124 156 Cuts at: 2302 2739 4114 5003 6632 8 Size: 437 1375 889 1629 43 Fragments arranged by size: 1629 1425 1375 889 437 275 192 156 124 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2146 2302 4114 1537 Size: 275 334 156 1812 4090 Fragments arranged by size: 4090 1812 334 275 156 EarI CTCTTCn'nnn_ Cuts at: 1353 2709 2982 3933 4597 1353 Size: 1356 273 951 664 3423 Fragments arranged by size: 3423 1356 951 664 273 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2700 5254 5628 5774 387 Size: 1064 138 1111 2554 374 146 1280 Fragments arranged by size: 2554 1280 1111 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2992 2992 Size: 6667 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2235 2451 3564 3630 3789 4730 5327 Size: 393 603 216 1113 66 159 941 597 Cuts at: 5327 6114 6382 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1113 941 787 603 597 393 268 216 159 66 EcoO109I rG'GnC_Cy Cuts at: 2896 3484 3920 2896 Size: 588 436 5643 Fragments arranged by size: 5643 588 436 EcoRI G'AATT_C Cuts at: 3105 3462 3976 4354 3105 Size: 357 514 378 5418 Fragments arranged by size: 5418 514 378 357 FspI TGC'GCA Cuts at: 5173 5173 Size: 6667 HaeII r_GCGC'y Cuts at: 1678 5814 1678 Size: 4136 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2410 2442 2956 2988 5308 Size: 32 135 32 838 32 514 32 2320 Cuts at: 5308 5340 5382 5414 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2320 838 514 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4011 4257 669 Size: 988 281 2073 246 3079 Fragments arranged by size: 3079 2073 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6667 HpaI GTT'AAC Cuts at: 4257 4257 Size: 6667 KpnI G_GTAC'C Cuts at: 3992 3992 Size: 6667 MfeI C'AATT_G Cuts at: 4266 4266 Size: 6667 MluI A'CGCG_T Cuts at: 6543 6543 Size: 6667 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2286 3021 3678 5781 5965 1305 Size: 981 735 657 2103 184 2007 Fragments arranged by size: 2103 2007 981 735 657 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2741 10 Size: 54 1425 343 909 3936 Fragments arranged by size: 3936 1425 909 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2459 2666 2763 3329 3831 4625 4984 Size: 648 1293 207 97 566 502 794 359 Cuts at: 4984 5143 518 Size: 159 2042 Fragments arranged by size: 2042 1293 794 648 566 502 359 207 159 97 MspA1I CmG'CkG Cuts at: 1606 2474 2618 3542 4083 4760 5908 6153 1606 Size: 868 144 924 541 677 1148 245 2120 Fragments arranged by size: 2120 1148 924 868 677 541 245 144 NcoI C'CATG_G Cuts at: 513 1065 2445 3783 513 Size: 552 1380 1338 3397 Fragments arranged by size: 3397 1380 1338 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6667 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6667 NotI GC'GGCC_GC Cuts at: 4114 4114 Size: 6667 NspI r_CATG'y Cuts at: 1132 1229 4025 5570 1132 Size: 97 2796 1545 2229 Fragments arranged by size: 2796 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6667 PciI A'CATG_T Cuts at: 5566 5566 Size: 6667 PflMI CCAn_nnn'nTGG Cuts at: 1108 3161 1108 Size: 2053 4614 PmeI GTTT'AAAC Cuts at: 3971 3971 Size: 6667 PmlI CAC'GTG Cuts at: 2310 2310 Size: 6667 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4736 4768 6249 6281 4736 Size: 32 1481 32 5122 Fragments arranged by size: 5122 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3484 3484 Size: 6667 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6667 PsiI TTA'TAA Cuts at: 4237 4237 Size: 6667 PspOMI G'GGCC_C Cuts at: 2895 2895 Size: 6667 PstI C_TGCA'G Cuts at: 830 4019 830 Size: 3189 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6667 PvuII CAG'CTG Cuts at: 2618 3542 2618 Size: 924 5743 SacI G_AGCT'C Cuts at: 2994 2994 Size: 6667 SalI G'TCGA_C Cuts at: 1936 4009 1936 Size: 2073 4594 SanDI GG'GwC_CC Cuts at: 3484 3484 Size: 6667 SapI GCTCTTCn'nnn_ Cuts at: 2982 2982 Size: 6667 SbfI CC_TGCA'GG Cuts at: 4019 4019 Size: 6667 ScaI AGT'ACT Cuts at: 4915 6627 4915 Size: 1712 4955 SexAI A'CCwGG_T Cuts at: 3243 3243 Size: 6667 SfcI C'TryA_G Cuts at: 826 1045 3183 3360 3795 4015 5150 5831 6022 Size: 219 2138 177 435 220 1135 681 191 Cuts at: 6022 826 Size: 1471 Fragments arranged by size: 2138 1471 1135 681 435 220 219 191 177 SfiI GGCCn_nnn'nGGCC Cuts at: 2894 2894 Size: 6667 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6667 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6667 SmaI CCC'GGG Cuts at: 1872 3994 1872 Size: 2122 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3668 4771 5672 5934 820 Size: 197 926 1725 1103 901 262 1553 Fragments arranged by size: 1725 1553 1103 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4458 493 Size: 3965 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6667 SphI G_CATG'C Cuts at: 1132 4025 1132 Size: 2893 3774 SspI AAT'ATT Cuts at: 5 52 4591 5 Size: 47 4539 2081 Fragments arranged by size: 4539 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6667 StyI C'CwwG_G Cuts at: 513 1065 2445 3317 3783 4106 513 Size: 552 1380 872 466 323 3074 Fragments arranged by size: 3074 1380 872 552 466 323 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2669 2753 4694 4711 4864 5049 5494 Size: 1255 1089 84 1941 17 153 185 445 Cuts at: 5494 325 Size: 1498 Fragments arranged by size: 1941 1498 1255 1089 445 185 153 84 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2811 4913 6625 Size: 274 80 33 51 1443 834 2102 1712 Cuts at: 6625 96 Size: 138 Fragments arranged by size: 2102 1712 1443 834 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6627 6659 6627 Size: 32 6635 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6667 XbaI T'CTAG_A Cuts at: 4003 4003 Size: 6667 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6667 XmaI C'CCGG_G Cuts at: 1870 3992 1870 Size: 2122 4545 XmnI GAAnn'nnTTC Cuts at: 3081 3882 4796 3081 Size: 801 914 4952 Fragments arranged by size: 4952 914 801 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4475 276 Size: 53 83 186 936 2941 2468 Fragments arranged by size: 2941 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SacII SfoI SrfI SwaI XcmI