Restriction map for
pFN21AB5550
(Linear) (Six-base) MAPSORT of: KIBB5550.seq Check: 6385 from: 1 to: 1962
KIBB5550.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 0 1009 1962
Size: 1009 953
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 446 1559 1625 1784 1962
Size: 230 216 1113 66 159 178
Fragments arranged by size:
1113 230 216 178 159 66
AleI CACnn'nnGTG
Cuts at: 0 1324 1962
Size: 1324 638
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 322 354 1962
Size: 322 32 1608
Fragments arranged by size:
1608 322 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1119 1363 1962
Size: 1119 244 599
Fragments arranged by size:
1119 599 244
ApaI G_GGCC'C
Cuts at: 0 894 1962
Size: 894 1068
ApoI r'AATT_y
Cuts at: 0 431 976 1100 1457 1962
Size: 431 545 124 357 505
Fragments arranged by size:
545 505 431 357 124
AvaI C'yCGr_G
Cuts at: 0 262 968 1962
Size: 262 706 994
Fragments arranged by size:
994 706 262
BanI G'GyrC_C
Cuts at: 0 1326 1962
Size: 1326 636
BanII G_rGCy'C
Cuts at: 0 894 989 1962
Size: 894 95 973
Fragments arranged by size:
973 894 95
BbsI GAAGACnn'nnnn_
Cuts at: 0 714 773 823 890 953 1547 1658 1847
Size: 714 59 50 67 63 594 111 189
Cuts at: 1847 1962
Size: 115
Fragments arranged by size:
714 594 189 115 111 67 63 59
50
BbvCI CC'TCA_GC
Cuts at: 0 470 1646 1962
Size: 470 1176 316
Fragments arranged by size:
1176 470 316
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 12 46 72 106 1962
Size: 12 34 26 34 1856
Fragments arranged by size:
1856 34 34 26 12
BciVI GTATCCnnnnn_n'
Cuts at: 0 1932 1962
Size: 1932 30
BclI T'GATC_A
Cuts at: 0 1221 1962
Size: 1221 741
BglI GCCn_nnn'nGGC
Cuts at: 0 889 1962
Size: 889 1073
BglII A'GATC_T
Cuts at: 0 1346 1962
Size: 1346 616
BlpI GC'TnA_GC
Cuts at: 0 37 1962
Size: 37 1925
Bme1580I G_kGCm'C
Cuts at: 0 85 894 1962
Size: 85 809 1068
Fragments arranged by size:
1068 809 85
BmgBI CAC'GTC
Cuts at: 0 78 1962
Size: 78 1884
Bpu10I CC'TnA_GC
Cuts at: 0 470 1646 1962
Size: 470 1176 316
Fragments arranged by size:
1176 470 316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1648 1962
Size: 1648 314
BsaI GGTCTCn'nnnn_
Cuts at: 0 952 1768 1962
Size: 952 816 194
Fragments arranged by size:
952 816 194
BsaAI yAC'GTr
Cuts at: 0 305 1962
Size: 305 1657
BsaBI GATnn'nnATC
Cuts at: 0 752 1573 1962
Size: 752 821 389
Fragments arranged by size:
821 752 389
BsaHI Gr'CG_yC
Cuts at: 0 281 1962
Size: 281 1681
BsaWI w'CCGG_w
Cuts at: 0 1004 1962
Size: 1004 958
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 405 435 1962
Size: 405 30 1527
Fragments arranged by size:
1527 405 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 73 1460 1962
Size: 70 3 1387 502
Fragments arranged by size:
1387 502 70 3
BseYI C'CCAG_C
Cuts at: 0 1743 1962
Size: 1743 219
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 874 1931 1962
Size: 343 531 1057 31
Fragments arranged by size:
1057 531 343 31
BsiEI CG_ry'CG
Cuts at: 0 144 300 1962
Size: 144 156 1662
Fragments arranged by size:
1662 156 144
BsiHKAI G_wGCw'C
Cuts at: 0 989 1214 1962
Size: 989 225 748
Fragments arranged by size:
989 748 225
Bsp1286I G_dGCh'C
Cuts at: 0 85 894 989 1214 1962
Size: 85 809 95 225 748
Fragments arranged by size:
809 748 225 95 85
BspHI T'CATG_A
Cuts at: 0 1295 1962
Size: 1295 667
BsrGI T'GTAC_A
Cuts at: 0 806 1962
Size: 806 1156
BssSI C'ACGA_G
Cuts at: 0 1472 1962
Size: 1472 490
BstBI TT'CG_AA
Cuts at: 0 725 1962
Size: 725 1237
BstEII G'GTnAC_C
Cuts at: 0 492 1234 1962
Size: 492 742 728
Fragments arranged by size:
742 728 492
BstYI r'GATC_y
Cuts at: 0 996 1346 1962
Size: 996 350 616
Fragments arranged by size:
996 616 350
BtgI C'CryG_G
Cuts at: 0 440 1155 1778 1962
Size: 440 715 623 184
Fragments arranged by size:
715 623 440 184
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 37 141 245 1962
Size: 37 104 104 1717
Fragments arranged by size:
1717 104 104 37
DrdI GACnn_nn'nnGTC
Cuts at: 0 718 781 1962
Size: 718 63 1181
Fragments arranged by size:
1181 718 63
EaeI y'GGCC_r
Cuts at: 0 17 141 297 734 1962
Size: 17 124 156 437 1228
Fragments arranged by size:
1228 437 156 124 17
EagI C'GGCC_G
Cuts at: 0 141 297 1962
Size: 141 156 1665
Fragments arranged by size:
1665 156 141
EarI CTCTTCn'nnn_
Cuts at: 0 704 977 1928 1962
Size: 704 273 951 34
Fragments arranged by size:
951 704 273 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 695 1962
Size: 695 1267
EcoICRI GAG'CTC
Cuts at: 0 987 1962
Size: 987 975
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 446 1559 1625 1784 1962
Size: 230 216 1113 66 159 178
Fragments arranged by size:
1113 230 216 178 159 66
EcoO109I rG'GnC_Cy
Cuts at: 0 891 1479 1915 1962
Size: 891 588 436 47
Fragments arranged by size:
891 588 436 47
EcoRI G'AATT_C
Cuts at: 0 1100 1457 1962
Size: 1100 357 505
Fragments arranged by size:
1100 505 357
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 405 437 951 983 1962
Size: 405 32 514 32 979
Fragments arranged by size:
979 514 405 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 1016 1673 1962
Size: 281 735 657 289
Fragments arranged by size:
735 657 289 281
MscI TGG'CCA
Cuts at: 0 736 1962
Size: 736 1226
MslI CAynn'nnrTG
Cuts at: 0 454 661 758 1324 1826 1962
Size: 454 207 97 566 502 136
Fragments arranged by size:
566 502 454 207 136 97
MspA1I CmG'CkG
Cuts at: 0 469 613 1537 1962
Size: 469 144 924 425
Fragments arranged by size:
924 469 425 144
NcoI C'CATG_G
Cuts at: 0 440 1778 1962
Size: 440 1338 184
Fragments arranged by size:
1338 440 184
PflMI CCAn_nnn'nTGG
Cuts at: 0 1156 1962
Size: 1156 806
PmlI CAC'GTG
Cuts at: 0 305 1962
Size: 305 1657
PpuMI rG'GwC_Cy
Cuts at: 0 1479 1962
Size: 1479 483
PspOMI G'GGCC_C
Cuts at: 0 890 1962
Size: 890 1072
PvuII CAG'CTG
Cuts at: 0 613 1537 1962
Size: 613 924 425
Fragments arranged by size:
924 613 425
SacI G_AGCT'C
Cuts at: 0 989 1962
Size: 989 973
SanDI GG'GwC_CC
Cuts at: 0 1479 1962
Size: 1479 483
SapI GCTCTTCn'nnn_
Cuts at: 0 977 1962
Size: 977 985
SexAI A'CCwGG_T
Cuts at: 0 1238 1962
Size: 1238 724
SfcI C'TryA_G
Cuts at: 0 1178 1355 1790 1962
Size: 1178 177 435 172
Fragments arranged by size:
1178 435 177 172
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 889 1962
Size: 889 1073
SmlI C'TyrA_G
Cuts at: 0 1663 1962
Size: 1663 299
StyI C'CwwG_G
Cuts at: 0 440 1312 1778 1962
Size: 440 872 466 184
Fragments arranged by size:
872 466 440 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 664 748 1962
Size: 664 84 1214
Fragments arranged by size:
1214 664 84
TatI w'GTAC_w
Cuts at: 0 806 1962
Size: 806 1156
XmnI GAAnn'nnTTC
Cuts at: 0 1076 1877 1962
Size: 1076 801 85
Fragments arranged by size:
1076 801 85
Enzymes that do cut:
AccI AcuI AleI AloI AlwNI ApaI ApoI AvaI
BanI BanII BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BsrGI BssSI BstBI BstEII BstYI
BtgI BtgZI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI Hin4I MmeI MscI MslI MspA1I
NcoI PflMI PmlI PpuMI PspOMI PvuII SacI SanDI
SapI SexAI SfcI SfiI SmlI StyI TaqII TatI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BfrBI BmrI BmtI BplI BpmI BsiWI
BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrFI BssHII
BstAPI BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraI
DraIII EcoNI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PfoI PmeI PpiI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacII SalI SbfI
ScaI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI