Restriction map for
pFN21AB3641
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglII BlpI BmyI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DpnII DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI Hin1I HincII HindII
HindIII HpaI HpaII KasI KpnI
Ksp632I KspI MaeII MamI MboI
MfeI MflI MluI MroI MspI
MunI NaeI NarI NciI NcoI
NdeII NgoMIV NheI NlaIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SapI Sau3AI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 636 CGCG CG^CG
AciI 741 GCGG CCGC(-3/-1)
AfaI 540 GTAC GT^AC
AvrII 30 CCTAGG C^CTAGG
BalI 119 TGGCCA TGG^CCA
BbvI 90 GCAGC GCAGC(8/12)
BglI 26 GCCTCCTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 30 CCTAGG C^CTAGG
BpmI 440 CTCCAG CTGGAG(16/14)
BsaI 214 GAGACC GGTCTC(1/5)
BseRI 586 GAGGAG GAGGAG(10/8)
BsiYI 657 CCGTGGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 657 CCGTGGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspHI 909 TCATGA T^CATGA
BspMI 416 ACCTGC ACCTGC(4/8)
BsrDI 761 GCAATG GCAATG(2/0)
BstUI 636 CGCG CG^CG
Cac8I 420 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 637 GCGC GCG^C
DraI 205 TTTAAA TTT^AAA
DraII 626 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 626 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 814 GAATTC G^AATTC
EcoT14I 30 CCTAGG C^C[AT][AT]GG
Fnu4HI 90 GCAGC GC^[ACGT]GC
HhaI 637 GCGC GCG^C
HinP1I 637 GCGC G^CGC
ItaI 90 GCAGC GC^[ACGT]GC
MaeIII 397 GTCAC ^GT[ACGT]AC
MluNI 119 TGGCCA TGG^CCA
MscI 119 TGGCCA TGG^CCA
MspA1I 685 CAGCTG C[AC]G^C[GT]G
MvnI 636 CGCG CG^CG
NdeI 464 CATATG CA^TATG
PpuMI 626 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 497 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 685 CAGCTG CAG^CTG
RcaI 909 TCATGA T^CATGA
RsaI 540 GTAC GT^AC
SfcI 551 CTACAG C^T[GA][CT]AG
StyI 30 CCTAGG C^C[AT][AT]GG
TaqI 313 TCGA T^CGA
TseI 90 GCAGC G^C[AT]GC
Tsp45I 397 GTCAC ^GT[GC]AC
TthHB8I 313 TCGA T^CGA
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 457 GTAGAC GT^[AC][GT]AC
859 GTAGAC
AlwNI 110 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
625 CAGGACCTG
AvaII 414 GGACC G^G[AT]CC
627 GGACC
BfaI 31 CTAG C^TAG
163 CTAG
BsmAI 214 GAGAC GTCTC(1/5)
366 GTCTC
BsmFI 650 GGGAC GGGAC(10/14)
448 GTCCC
BsmI 174 GAATGC GAATGC(1/-1)
768 GCATTC
BssKI 297 CCAGG ^CC[ACGT]GG
624 CCAGG
BstNI 297 CCAGG CC^[AT]GG
624 CCAGG
Cfr13I 414 GGACC G^G[ACGT]CC
627 GGACC
DsaI 410 CCACGG C^C[GA][CT]GG
657 CCGTGG
EaeI 119 TGGCCA [CT]^GGCC[GA]
471 TGGCCG
EcoRII 297 CCAGG ^CC[AT]GG
624 CCAGG
MaeI 31 CTAG C^TAG
163 CTAG
MseI 56 TTAA T^TAA
206 TTAA
MvaI 297 CCAGG CC^[AT]GG
624 CCAGG
MwoI 26 GCCTCCTAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
601 GCATACAAGGC
Sau96I 414 GGACC G^G[ACGT]CC
627 GGACC
ScrFI 297 CCAGG CC^[ACGT]GG
624 CCAGG
Tru9I 56 TTAA T^TAA
206 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AcsI AluI ApoI BsaJI BsrI
BstF5I DdeI FokI HaeIII HinfI
HphI MboII MnlI MslI NlaIII
PleI SfaNI TfiI Tsp509I TspRI