Restriction map for
pFN21AB3264
##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspI AvrII BanII BbeI
BbrPI BbvCI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BssSI
Bst1107I BstZ17I CelII Cfr10I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV FauI
FbaI FseI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MluI MroI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NspI PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tth111I
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 1675 GTAGAC GT^[AC][GT]AC
AflIII 987 ACGTGT A^C[GA][CT]GT
AlwNI 1015 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 1649 GAGCAC G[AT]GC[AT]^C
AvaI 1565 CTCGAG C^[CT]CG[GA]G
AviII 99 TGCGCA TGC^GCA
BalI 365 TGGCCA TGG^CCA
BamHI 1770 GGATCC G^GATCC
BanI 657 GGCACC G^G[CT][GA]CC
BbsI 412 GAAGAC GAAGAC(2/6)
BcgI 2372 GCATTGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1866 GGATAC GTATCC(6/5)
BpuAI 412 GAAGAC GAAGAC(2/6)
BsiHKAI 1649 GAGCAC G[AT]GC[AT]^C
BsmBI 250 GAGACG CGTCTC(1/5)
BsoBI 1565 CTCGAG C^[CT]CG[GA]G
BspHI 756 TCATGA T^CATGA
BspMI 1791 GCAGGT ACCTGC(4/8)
BssHII 20 GCGCGC G^CGCGC
BstAPI 1580 GCAAAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1220 TTCGAA TT^CGAA
BstEII 66 GGTGACC G^GT[ACGT]ACC
BstPI 66 GGTGACC G^GT[ACGT]ACC
BstXI 2519 CCACATATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 61 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2097 GACCAAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 7 CCGTGG C^C[GA][CT]GG
EaeI 365 TGGCCA [CT]^GGCC[GA]
EcoO65I 66 GGTGACC G^GT[ACGT]ACC
EcoT14I 1422 CCAAGG C^C[AT][AT]GG
EcoT22I 1552 ATGCAT ATGCA^T
FspI 99 TGCGCA TGC^GCA
MfeI 1680 CAATTG C^AATTG
MluNI 365 TGGCCA TGG^CCA
MscI 365 TGGCCA TGG^CCA
MunI 1680 CAATTG C^AATTG
NdeI 2522 CATATG CA^TATG
NsiI 1552 ATGCAT ATGCA^T
NspV 1220 TTCGAA TT^CGAA
PaeR7I 1565 CTCGAG C^TCGAG
PflMI 1014 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1839 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 756 TCATGA T^CATGA
SfuI 1220 TTCGAA TT^CGAA
SspI 647 AATATT AAT^ATT
StuI 426 AGGCCT AGG^CCT
StyI 1422 CCAAGG C^C[AT][AT]GG
Van91I 1014 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1233 TCTAGA T^CTAGA
XhoI 1565 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 21 CGCG CG^CG
112 CGCG
AciI 111 CCGC CCGC(-3/-1)
190 CCGC
AseI 403 ATTAAT AT^TAAT
2059 ATTAAT
AsnI 403 ATTAAT AT^TAAT
2059 ATTAAT
BglII 471 AGATCT A^GATCT
1335 AGATCT
BmyI 1649 GAGCAC G[AGT]GC[ACT]^C
1981 GTGCCC
BsaI 1126 GAGACC GGTCTC(1/5)
2047 GAGACC
BseRI 1009 CTCCTC GAGGAG(10/8)
2483 GAGGAG
Bsp1286I 1649 GAGCAC G[AGT]GC[ACT]^C
1981 GTGCCC
BstUI 21 CGCG CG^CG
112 CGCG
DraII 379 AGGCCCT [GA]G^G[ACGT]CC[CT]
1839 AGGACCC
EcoO109I 379 AGGCCCT [GA]G^G[ACGT]CC[CT]
1839 AGGACCC
MaeII 253 ACGT A^CGT
987 ACGT
MslI 2454 CACAAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2520 CACATATGTG
MspA1I 133 CAGCTG C[AC]G^C[GT]G
2315 CAGCTG
MvnI 21 CGCG CG^CG
112 CGCG
PshBI 403 ATTAAT AT^TAAT
2059 ATTAAT
PstI 843 CTGCAG CTGCA^G
2284 CTGCAG
PvuII 133 CAGCTG CAG^CTG
2315 CAGCTG
SmlI 1565 CTCGAG C^T[CT][GA]AG
2251 CTCAAG
XcmI 1079 CCATGAAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1422 CCAAGGACAAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AcsI AfaI AluI AlwI AocI
ApoI AvaII BbvI BcnI BfaI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI BsrDI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI EarI
Eco81I EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII