Restriction map for
pFN21AB3264
(Circular) (Six-base) MAPSORT of: pFN21AB3264.seq Check: 7946 from: 1 to: 7246
pFN21AB3264 7246 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5056 278
Size: 53 83 186 936 3520 2468
Fragments arranged by size:
3520 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3681 4589 1937
Size: 1744 908 4594
Fragments arranged by size:
4594 1744 908
Acc65I G'GTAC_C
Cuts at: 4567 4567
Size: 7246
AclI AA'CG_TT
Cuts at: 1563 5373 5746 1563
Size: 3810 373 3063
Fragments arranged by size:
3810 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2195 3329 5309 6693 6961 1632
Size: 563 1134 1980 1384 268 1917
Fragments arranged by size:
1980 1917 1384 1134 563 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7049
AflIII A'CryG_T
Cuts at: 1203 2992 6145 7122 1203
Size: 1789 3153 977 1327
Fragments arranged by size:
3153 1789 1327 977
AgeI A'CCGG_T
Cuts at: 6077 6077
Size: 7246
AhdI GACnn_n'nnGTC
Cuts at: 5975 5975
Size: 7246
AleI CACnn'nnGTG
Cuts at: 1166 4529 1166
Size: 3363 3883
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2069 1184
Size: 885 6361
AlwNI CAG_nnn'CTG
Cuts at: 3025 4989 6561 3025
Size: 1964 1572 3710
Fragments arranged by size:
3710 1964 1572
ApaLI G'TGCA_C
Cuts at: 5303 6459 5303
Size: 1156 6090
ApoI r'AATT_y
Cuts at: 1493 2693 2954 3172 3222 3446 3925 4208 4555
Size: 1200 261 218 50 224 479 283 347
Cuts at: 4555 4781 4933 7042 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1200 479 347 283 261 226
224 218 152 50
AseI AT'TA_AT
Cuts at: 160 1032 2409 4065 5800 160
Size: 872 1377 1656 1735 1606
Fragments arranged by size:
1735 1656 1606 1377 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7246
AvaI C'yCGr_G
Cuts at: 1870 1943 3570 4571 1870
Size: 73 1627 1001 4545
Fragments arranged by size:
4545 1627 1001 73
BamHI G'GATC_C
Cuts at: 3775 4576 4943 3775
Size: 801 367 6078
Fragments arranged by size:
6078 801 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2662 4567 6022 618
Size: 325 217 627 875 1905 1455 1842
Fragments arranged by size:
1905 1842 1455 875 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4565 1391
Size: 3174 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2424 928
Size: 971 525 5750
Fragments arranged by size:
5750 971 525
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4366 4400 5437 5471 1903
Size: 34 2429 34 1037 34 3678
Fragments arranged by size:
3678 2429 1037 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3865 5138 6348 3865
Size: 1273 1210 4763
Fragments arranged by size:
4763 1273 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7246
BfrBI ATG'CAT
Cuts at: 3559 3559
Size: 7246
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5857 136
Size: 107 122 71 631 605 4185 1525
Fragments arranged by size:
4185 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2476 3340 7241 2476
Size: 864 3901 2481
Fragments arranged by size:
3901 2481 864
BlpI GC'TnA_GC
Cuts at: 4663 4663
Size: 7246
Bme1580I G_kGCm'C
Cuts at: 1790 3990 5307 6463 1790
Size: 2200 1317 1156 2573
Fragments arranged by size:
2573 2200 1317 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7246
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2200 4977 5935
Size: 416 53 83 399 24 783 2777 958
Cuts at: 5935 442
Size: 1753
Fragments arranged by size:
2777 1753 958 783 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7246
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2147 2147
Size: 7246
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3002 3845 3957 4047 5906 1239
Size: 1763 843 112 90 1859 2579
Fragments arranged by size:
2579 1859 1763 843 112 90
Bpu10I CC'TnA_GC
Cuts at: 2088 2088
Size: 7246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4241 5371 6236 6534 4241
Size: 1130 865 298 4953
Fragments arranged by size:
4953 1130 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3125 4046 5909 882
Size: 617 1626 921 1863 2219
Fragments arranged by size:
2219 1863 1626 921 617
BsaAI yAC'GTr
Cuts at: 493 1204 5037 493
Size: 711 3833 2702
Fragments arranged by size:
3833 2702 711
BsaBI GATnn'nnATC
Cuts at: 7240 7240
Size: 7246
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5053 5435 6070 275
Size: 53 83 186 936 3520 382 635 1451
Fragments arranged by size:
3520 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5679 6077 6351 6498 6909 5679
Size: 398 274 147 411 6016
Fragments arranged by size:
6016 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4431 4461 1178
Size: 30 165 30 3028 30 3963
Fragments arranged by size:
3963 3028 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3005 4503 1186
Size: 1819 1498 3929
Fragments arranged by size:
3929 1819 1498
BseYI C'CCAG_C
Cuts at: 3829 4372 6449 6945 3829
Size: 543 2077 496 4130
Fragments arranged by size:
4130 2077 543 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6673
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4696 5457 6485 1540
Size: 275 186 2695 761 1028 2301
Fragments arranged by size:
2695 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3658 5307 5392 6463 3658
Size: 1649 85 1071 4441
Fragments arranged by size:
4441 1649 1071 85
BsmI GAATG_Cn'
Cuts at: 4757 4850 4757
Size: 93 7153
BsmBI CGTCTCn'nnnn_
Cuts at: 2249 2249
Size: 7246
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3658 3990 4565 5307 5392 6463 1391
Size: 399 1868 332 575 742 85 1071 2174
Fragments arranged by size:
2174 1868 1071 742 575 399 332 85
BspEI T'CCGG_A
Cuts at: 6909 6909
Size: 7246
BspHI T'CATG_A
Cuts at: 2761 5135 6865 2761
Size: 2374 1730 3142
Fragments arranged by size:
3142 2374 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3787 4601 844
Size: 2943 814 3489
Fragments arranged by size:
3489 2943 814
BsrBI CCG'CTC
Cuts at: 1394 1823 5133 1394
Size: 429 3310 3507
Fragments arranged by size:
3507 3310 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2041 4038 4191 4377 5741 5915 65
Size: 1183 793 1997 153 186 1364 174 1396
Fragments arranged by size:
1997 1396 1364 1183 793 186 174 153
BsrFI r'CCGG_y
Cuts at: 1700 1810 5890 6077 1700
Size: 110 4080 187 2869
Fragments arranged by size:
4080 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7246
BssHII G'CGCG_C
Cuts at: 1925 2025 1925
Size: 100 7146
BssSI C'ACGA_G
Cuts at: 5306 6318 5306
Size: 1012 6234
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3591 794
Size: 2797 4449
BstBI TT'CG_AA
Cuts at: 3226 6050 3226
Size: 2824 4422
BstEII G'GTnAC_C
Cuts at: 1184 2071 1184
Size: 887 6359
BstXI CCAn_nnnn'nTGG
Cuts at: 4531 4531
Size: 7246
BstYI r'GATC_y
Cuts at: 1972 2476 3340 3775 4576 4943 5328 5345 6797
Size: 504 864 435 801 367 385 17 1452
Cuts at: 6797 6883 6895 7241 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 864 801 504 435 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2189 3207 3605 2189
Size: 1018 398 5830
Fragments arranged by size:
5830 1018 398
BtgI C'CryG_G
Cuts at: 513 1065 2004 2012 513
Size: 552 939 8 5747
Fragments arranged by size:
5747 939 552 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2133 4369 487
Size: 683 56 907 2236 3364
Fragments arranged by size:
3364 2236 907 683 56
BtsI GCAGTG_nn'
Cuts at: 2027 2036 2857 3294 4762 5556 5576 2027
Size: 9 821 437 1468 794 20 3697
Fragments arranged by size:
3697 1468 821 794 437 20 9
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7246
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7211
DraI TTT'AAA
Cuts at: 3000 3471 4213 4550 3000
Size: 471 742 337 5696
Fragments arranged by size:
5696 742 471 337
DraIII CAC_nnn'GTG
Cuts at: 2071 2071
Size: 7246
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4108 6253 809
Size: 507 2792 2145 1802
Fragments arranged by size:
2792 2145 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2370 4693 5582
Size: 54 1425 50 275 18 540 2323 889
Cuts at: 5582 7211 8
Size: 1629 43
Fragments arranged by size:
2323 1629 1425 889 540 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4693 1537
Size: 275 2881 4090
Fragments arranged by size:
4090 2881 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2967 3670 4530 5176 1353
Size: 1614 703 860 646 3423
Fragments arranged by size:
3423 1614 860 703 646
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5833 6207 6353 387
Size: 1064 138 4244 374 146 1280
Fragments arranged by size:
4244 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2195 3002 3329 3845 3957 4047 5309
Size: 393 563 807 327 516 112 90 1262
Cuts at: 5309 5906 6693 6961 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1262 807 787 597 563 516 393
327 268 112 90
EcoO109I rG'GnC_Cy
Cuts at: 2385 3845 2385
Size: 1460 5786
EcoRI G'AATT_C
Cuts at: 4555 4933 4555
Size: 378 6868
FspI TGC'GCA
Cuts at: 2106 5752 2106
Size: 3646 3600
HaeII r_GCGC'y
Cuts at: 1678 6393 1678
Size: 4715 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5887 5919 5961 5993 1373
Size: 32 135 32 4315 32 42 32 2626
Fragments arranged by size:
4315 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4590 4836 669
Size: 988 281 2652 246 3079
Fragments arranged by size:
3079 2652 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7246
HpaI GTT'AAC
Cuts at: 4836 4836
Size: 7246
KpnI G_GTAC'C
Cuts at: 4571 4571
Size: 7246
MfeI C'AATT_G
Cuts at: 3685 4845 3685
Size: 1160 6086
MluI A'CGCG_T
Cuts at: 7122 7122
Size: 7246
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3071 3925 6360 6544 1305
Size: 1766 854 2435 184 2007
Fragments arranged by size:
2435 2007 1766 854 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2372 10
Size: 54 1425 343 540 4884
Fragments arranged by size:
4884 1425 540 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4463 4529 5204 5563 5722 518
Size: 648 3297 66 675 359 159 2042
Fragments arranged by size:
3297 2042 675 648 359 159 66
MspA1I CmG'CkG
Cuts at: 1606 2140 4322 4662 5339 6487 6732 1606
Size: 534 2182 340 677 1148 245 2120
Fragments arranged by size:
2182 2120 1148 677 534 340 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5755
Fragments arranged by size:
5755 939 552
NdeI CA'TA_TG
Cuts at: 387 4528 387
Size: 4141 3105
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7246
NotI GC'GGCC_GC
Cuts at: 4693 4693
Size: 7246
NsiI A_TGCA'T
Cuts at: 3561 3561
Size: 7246
NspI r_CATG'y
Cuts at: 1132 1229 4604 6149 1132
Size: 97 3375 1545 2229
Fragments arranged by size:
3375 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7246
PciI A'CATG_T
Cuts at: 6145 6145
Size: 7246
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3025 1108
Size: 1917 5329
PfoI T'CCnGG_A
Cuts at: 3018 3018
Size: 7246
PmeI GTTT'AAAC
Cuts at: 4550 4550
Size: 7246
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5315 5347 6828 6860 5315
Size: 32 1481 32 5701
Fragments arranged by size:
5701 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3845 3845
Size: 7246
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7246
PsiI TTA'TAA
Cuts at: 4816 4816
Size: 7246
PspXI vC'TCGA_Gb
Cuts at: 3570 3570
Size: 7246
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4264 4296 4264
Size: 32 7214
PstI C_TGCA'G
Cuts at: 830 2852 4293 4598 830
Size: 2022 1441 305 3478
Fragments arranged by size:
3478 2022 1441 305
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7246
PvuII CAG'CTG
Cuts at: 2140 4322 2140
Size: 2182 5064
SalI G'TCGA_C
Cuts at: 1936 4588 1936
Size: 2652 4594
SbfI CC_TGCA'GG
Cuts at: 4598 4598
Size: 7246
ScaI AGT'ACT
Cuts at: 2846 4081 4238 5494 7206 2846
Size: 1235 157 1256 1712 2886
Fragments arranged by size:
2886 1712 1256 1235 157
SfcI C'TryA_G
Cuts at: 826 1045 2361 2848 3165 4289 4594 5729 6410
Size: 219 1316 487 317 1124 305 1135 681
Cuts at: 6410 6601 826
Size: 191 1471
Fragments arranged by size:
1471 1316 1135 1124 681 487 317 305
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7246
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7246
SmaI CCC'GGG
Cuts at: 1872 4573 1872
Size: 2701 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3570 4256 5350 6251 6513 820
Size: 197 926 1627 686 1094 901 262 1553
Fragments arranged by size:
1627 1553 1094 926 901 686 262 197
SnaBI TAC'GTA
Cuts at: 493 5037 493
Size: 4544 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7246
SphI G_CATG'C
Cuts at: 1132 4604 1132
Size: 3472 3774
SspI AAT'ATT
Cuts at: 5 52 2654 5170 5
Size: 47 2602 2516 2081
Fragments arranged by size:
2602 2516 2081 47
StuI AGG'CCT
Cuts at: 1517 2433 1517
Size: 916 6330
StyI C'CwwG_G
Cuts at: 513 1065 2004 3427 4685 513
Size: 552 939 1423 1258 3074
Fragments arranged by size:
3074 1423 1258 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4070 5273 5290 5443 5628 6073 325
Size: 1255 2490 1203 17 153 185 445 1498
Fragments arranged by size:
2490 1498 1255 1203 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2844 3351 4079
Size: 274 80 33 51 1443 867 507 728
Cuts at: 4079 4137 4236 5492 7204 96
Size: 58 99 1256 1712 138
Fragments arranged by size:
1712 1443 1256 867 728 507 274 138
99 80 58 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4431 4463 7206 7238 4431
Size: 32 2743 32 4439
Fragments arranged by size:
4439 2743 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7246
XbaI T'CTAG_A
Cuts at: 3238 4582 3238
Size: 1344 5902
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3091 3434 3091
Size: 343 6903
XhoI C'TCGA_G
Cuts at: 1943 3570 1943
Size: 1627 5619
XmaI C'CCGG_G
Cuts at: 1870 4571 1870
Size: 2701 4545
XmnI GAAnn'nnTTC
Cuts at: 5375 5375
Size: 7246
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5054 276
Size: 53 83 186 936 3520 2468
Fragments arranged by size:
3520 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BsiWI BstZ17I EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspOMI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI