Restriction map for
pFN21AB5823
##################################
# NO CUT ENZYMES ( 186 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BanI BanII BbeI
BbrPI BbsI BbvCI BbvI BcgI
BciVI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiWI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssKI Bst1107I BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI DsaI Eam1105I Eco47III
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRII EcoRV EcoT14I EcoT22I FauI
Fnu4HI FokI FseI FspI HaeII
HgaI Hin1I HincII HindII HinfI
HpaI HphI ItaI KasI KpnI
KspI MaeIII MfeI MluI MluNI
MroI MscI MspA1I MunI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NotI NruI
NsiI NspI PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI ScrFI SexAI SfcI SfiI
SfoI SgrAI SmaI SmlI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI TfiI TseI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AcsI 79 GAATTT [GA]^AATT[CT]
AfaI 382 GTAC GT^AC
Alw44I 160 GTGCAC G^TGCAC
ApaLI 160 GTGCAC G^TGCAC
ApoI 79 GAATTT [GA]^AATT[CT]
Asp700I 355 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 348 GGATCC G^GATCC
BclI 140 TGATCA T^GATCA
BfaI 11 CTAG C^TAG
BpmI 70 CTGGAG CTGGAG(16/14)
BsaAI 34 TACGTA [CT]AC^GT[GA]
BsaBI 331 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 85 CGGCCG CG[GA][CT]^CG
BspHI 137 TCATGA T^CATGA
BspMI 23 ACCTGC ACCTGC(4/8)
BssSI 61 CACGAG CACGAG(-5/-1)
BstBI 76 TTCGAA TT^CGAA
BstYI 348 GGATCC [GA]^GATC[CT]
Cac8I 10 GCTAGC GC[ACGT]^[ACGT]GC
CfoI 27 GCGC GCG^C
DdeI 100 CTTAG C^T[ACGT]AG
EagI 85 CGGCCG C^GGCCG
EarI 186 GAAGAG CTCTTC(1/4)
EclXI 85 CGGCCG C^GGCCG
Eco52I 85 CGGCCG C^GGCCG
FbaI 140 TGATCA T^GATCA
HapII 88 CCGG C^CGG
HhaI 27 GCGC GCG^C
HinP1I 27 GCGC G^CGC
HindIII 97 AAGCTT A^AGCTT
HpaII 88 CCGG C^CGG
Ksp632I 186 GAAGAG CTCTTC(1/4)
MaeI 11 CTAG C^TAG
MaeII 35 ACGT A^CGT
MamI 331 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 348 GGATCC [GA]^GATC[CT]
MseI 434 TTAA T^TAA
MslI 163 CACAAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 88 CCGG C^CGG
NheI 10 GCTAGC G^CTAGC
NlaIII 138 CATG CATG^
NlaIV 348 GGATCC GG[ACGT]^[ACGT]CC
NspV 76 TTCGAA TT^CGAA
RcaI 137 TCATGA T^CATGA
RsaI 382 GTAC GT^AC
SfaNI 226 GATGC GCATC(5/9)
SfuI 76 TTCGAA TT^CGAA
SnaBI 34 TACGTA TAC^GTA
StuI 357 AGGCCT AGG^CCT
Tru9I 434 TTAA T^TAA
TspRI 204 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 348 GGATCC [GA]^GATC[CT]
XmnI 355 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AluI 98 AGCT AG^CT
266 AGCT
AlwI 349 GATCC GGATC(4/5)
348 GGATC
AspHI 160 GTGCAC G[AT]GC[AT]^C
289 GAGCAC
BsiHKAI 160 GTGCAC G[AT]GC[AT]^C
289 GAGCAC
BsiYI 242 CCCCTGATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
243 CCCTGATAGGG
BslI 242 CCCCTGATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
243 CCCTGATAGGG
DpnII 141 GATC ^GATC
349 GATC
EaeI 85 CGGCCG [CT]^GGCC[GA]
255 TGGCCG
MboI 141 GATC ^GATC
349 GATC
MnlI 238 CCTC CCTC(7/6)
151 GAGG
NdeII 141 GATC ^GATC
349 GATC
Sau3AI 141 GATC ^GATC
349 GATC
TaqI 77 TCGA T^CGA
363 TCGA
TthHB8I 77 TCGA T^CGA
363 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 5 enzymes) #
#########################################
BmyI Bsp1286I HaeIII MboII Tsp509I