(Circular) (Six-base) MAPSORT of: pFN21AB9407.seq Check: 1152 from: 1 to: 8479 pFN21AB9407 8479 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4333 4450 4333 Size: 117 8362 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6289 278 Size: 53 83 186 936 4753 2468 Fragments arranged by size: 4753 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2654 3141 5822 1937 Size: 717 487 2681 4594 Fragments arranged by size: 4594 2681 717 487 Acc65I G'GTAC_C Cuts at: 5800 5800 Size: 8479 AclI AA'CG_TT Cuts at: 1563 6606 6979 1563 Size: 5043 373 3063 Fragments arranged by size: 5043 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2618 3353 5720 6542 7926 8194 1632 Size: 986 735 2367 822 1384 268 1917 Fragments arranged by size: 2367 1917 1384 986 822 735 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8282 AflIII A'CryG_T Cuts at: 1203 7378 8355 1203 Size: 6175 977 1327 Fragments arranged by size: 6175 1327 977 AgeI A'CCGG_T Cuts at: 7310 7310 Size: 8479 AhdI GACnn_n'nnGTC Cuts at: 7208 7208 Size: 8479 AleI CACnn'nnGTG Cuts at: 1166 4304 4988 1166 Size: 3138 684 4657 Fragments arranged by size: 4657 3138 684 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4322 4758 5006 1184 Size: 3138 436 248 4657 Fragments arranged by size: 4657 3138 436 248 AlwNI CAG_nnn'CTG Cuts at: 6222 7794 6222 Size: 1572 6907 ApaI G_GGCC'C Cuts at: 2189 4399 2189 Size: 2210 6269 ApaLI G'TGCA_C Cuts at: 6536 7692 6536 Size: 1156 7323 ApoI r'AATT_y Cuts at: 1493 4492 5604 5788 6014 6166 8275 1493 Size: 2999 1112 184 226 152 2109 1697 Fragments arranged by size: 2999 2109 1697 1112 226 184 152 AseI AT'TA_AT Cuts at: 160 1032 4673 7033 160 Size: 872 3641 2360 1606 Fragments arranged by size: 3641 2360 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8479 AvaI C'yCGr_G Cuts at: 1870 1943 2188 2513 4313 4921 5649 5804 1870 Size: 73 245 325 1800 608 728 155 4545 Fragments arranged by size: 4545 1800 728 608 325 245 155 73 BamHI G'GATC_C Cuts at: 5809 6176 5809 Size: 367 8112 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2543 3003 3644 4038 4795 Size: 325 217 627 756 460 641 394 757 Cuts at: 4795 4816 5800 7255 618 Size: 21 984 1455 1842 Fragments arranged by size: 1842 1455 984 757 756 641 627 460 394 325 217 21 BanII G_rGCy'C Cuts at: 1391 2189 2391 4202 4399 4922 5147 5798 1391 Size: 798 202 1811 197 523 225 651 4072 Fragments arranged by size: 4072 1811 798 651 523 225 202 197 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3120 3164 928 Size: 971 1221 44 6243 Fragments arranged by size: 6243 1221 971 44 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6670 6704 1903 Size: 34 4733 34 3678 Fragments arranged by size: 4733 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6371 7581 6371 Size: 1210 7269 BclI T'GATC_A Cuts at: 1548 2380 2607 1548 Size: 832 227 7420 Fragments arranged by size: 7420 832 227 BfrBI ATG'CAT Cuts at: 5099 5099 Size: 8479 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7090 136 Size: 107 122 71 631 605 5418 1525 Fragments arranged by size: 5418 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3621 8474 3621 Size: 4853 3626 BlpI GC'TnA_GC Cuts at: 5551 5896 5551 Size: 345 8134 Bme1580I G_kGCm'C Cuts at: 1790 2189 4399 4800 4821 6540 7696 1790 Size: 399 2210 401 21 1719 1156 2573 Fragments arranged by size: 2573 2210 1719 1156 401 399 21 BmgBI CAC'GTC Cuts at: 1318 2260 3856 4759 5005 1318 Size: 942 1596 903 246 4792 Fragments arranged by size: 4792 1596 942 903 246 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2932 4393 4637 Size: 416 53 83 399 24 1515 1461 244 Cuts at: 4637 6210 7168 442 Size: 1573 958 1753 Fragments arranged by size: 1753 1573 1515 1461 958 416 399 244 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3792 1056 Size: 2736 5743 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2544 4837 2544 Size: 2293 6186 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2237 2375 3482 4326 4564 4683 5760 7139 Size: 998 138 1107 844 238 119 1077 1379 Cuts at: 7139 1239 Size: 2579 Fragments arranged by size: 2579 1379 1107 1077 998 844 238 138 119 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3227 3594 6604 7469 7767 3227 Size: 367 3010 865 298 3939 Fragments arranged by size: 3939 3010 865 367 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2379 3764 5172 5618 7142 882 Size: 617 880 1385 1408 446 1524 2219 Fragments arranged by size: 2219 1524 1408 1385 880 617 446 BsaAI yAC'GTr Cuts at: 493 1204 6270 493 Size: 711 5066 2702 Fragments arranged by size: 5066 2702 711 BsaBI GATnn'nnATC Cuts at: 8473 8473 Size: 8479 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4691 6286 6668 7303 Size: 53 83 186 936 3158 1595 382 635 Cuts at: 7303 275 Size: 1451 Fragments arranged by size: 3158 1595 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5181 6912 7310 7584 7731 8142 5181 Size: 1731 398 274 147 411 5518 Fragments arranged by size: 5518 1731 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2114 2144 2431 2461 4293 Size: 30 165 30 711 30 287 30 1832 Cuts at: 4293 4323 1178 Size: 30 5334 Fragments arranged by size: 5334 1832 711 287 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2735 2738 2798 3080 4188 4822 5496 5526 Size: 1549 3 60 282 1108 634 674 30 Cuts at: 5526 1186 Size: 4139 Fragments arranged by size: 4139 1549 1108 674 634 282 60 30 3 BseYI C'CCAG_C Cuts at: 2086 2101 2194 2263 3847 7682 8178 2086 Size: 15 93 69 1584 3835 496 2387 Fragments arranged by size: 3835 2387 1584 496 93 69 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2243 4112 4259 5012 1163 Size: 573 507 1869 147 753 4630 Fragments arranged by size: 4630 1869 753 573 507 147 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2154 4651 5929 6690 7718 1540 Size: 275 186 153 2497 1278 761 1028 2301 Fragments arranged by size: 2497 2301 1278 1028 761 275 186 153 BsiHKAI G_wGCw'C Cuts at: 2118 2391 2790 4311 4922 6540 6625 7696 2118 Size: 273 399 1521 611 1618 85 1071 2901 Fragments arranged by size: 2901 1618 1521 1071 611 399 273 85 BsiWI C'GTAC_G Cuts at: 2130 2130 Size: 8479 BsmI GAATG_Cn' Cuts at: 2060 5308 5644 5990 6083 2060 Size: 3248 336 346 93 4456 Fragments arranged by size: 4456 3248 346 336 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5703 5703 Size: 8479 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2189 2391 2790 4202 4311 4399 Size: 399 328 71 202 399 1412 109 88 Cuts at: 4399 4800 4821 4922 5147 5798 6540 6625 7696 Size: 401 21 101 225 651 742 85 1071 Cuts at: 7696 1391 Size: 2174 Fragments arranged by size: 2174 1412 1071 742 651 401 399 399 328 225 202 109 101 88 85 71 21 BspEI T'CCGG_A Cuts at: 8142 8142 Size: 8479 BspHI T'CATG_A Cuts at: 2383 6368 8098 2383 Size: 3985 1730 2764 Fragments arranged by size: 3985 2764 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2346 4333 4450 5834 844 Size: 1502 1987 117 1384 3489 Fragments arranged by size: 3489 1987 1502 1384 117 BsrBI CCG'CTC Cuts at: 1394 1823 2141 6366 1394 Size: 429 318 4225 3507 Fragments arranged by size: 4225 3507 429 318 BsrDI GCAATG_nn' Cuts at: 65 1248 3680 5101 6974 7148 65 Size: 1183 2432 1421 1873 174 1396 Fragments arranged by size: 2432 1873 1421 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4855 7123 7310 1700 Size: 110 3045 2268 187 2869 Fragments arranged by size: 3045 2869 2268 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8479 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8479 BssSI C'ACGA_G Cuts at: 6539 7551 6539 Size: 1012 7467 BstAPI GCAn_nnn'nTGC Cuts at: 794 5096 794 Size: 4302 4177 BstBI TT'CG_AA Cuts at: 7283 7283 Size: 8479 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8479 BstYI r'GATC_y Cuts at: 1972 3621 4060 4378 5010 5184 5809 6176 6561 Size: 1649 439 318 632 174 625 367 385 Cuts at: 6561 6578 8030 8116 8128 8474 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1649 1452 632 625 439 385 367 346 318 174 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4140 4655 4140 Size: 515 7964 BtgI C'CryG_G Cuts at: 513 1065 4986 513 Size: 552 3921 4006 Fragments arranged by size: 4006 3921 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2818 487 Size: 683 56 1592 6148 Fragments arranged by size: 6148 1592 683 56 BtsI GCAGTG_nn' Cuts at: 4957 5995 6789 6809 4957 Size: 1038 794 20 6627 Fragments arranged by size: 6627 1038 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8479 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4477 4512 529 Size: 35 3913 35 4496 Fragments arranged by size: 4496 3913 35 35 DraI TTT'AAA Cuts at: 4387 5783 4387 Size: 1396 7083 DraIII CAC_nnn'GTG Cuts at: 4631 4631 Size: 8479 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7486 809 Size: 507 6170 1802 Fragments arranged by size: 6170 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4003 4363 4648 Size: 54 1425 50 275 18 2173 360 285 Cuts at: 4648 5677 5926 6815 8444 8 Size: 1029 249 889 1629 43 Fragments arranged by size: 2173 1629 1425 1029 889 360 285 275 249 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4648 5926 1537 Size: 275 2836 1278 4090 Fragments arranged by size: 4090 2836 1278 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2535 3027 4190 4866 6409 1353 Size: 1182 492 1163 676 1543 3423 Fragments arranged by size: 3423 1543 1182 1163 676 492 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7066 7440 7586 387 Size: 1064 138 5477 374 146 1280 Fragments arranged by size: 5477 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2389 4920 2389 Size: 2531 5948 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2237 2375 2618 3353 3482 4326 4564 Size: 393 605 138 243 735 129 844 238 Cuts at: 4564 4683 5720 5760 6542 7139 7926 8194 1239 Size: 119 1037 40 782 597 787 268 1524 Fragments arranged by size: 1524 1037 844 787 782 735 605 597 393 268 243 238 138 129 119 40 EcoNI CCTnn'n_nnAGG Cuts at: 5246 5246 Size: 8479 EcoO109I rG'GnC_Cy Cuts at: 2072 2185 2335 2906 4096 5373 2072 Size: 113 150 571 1190 1277 5178 Fragments arranged by size: 5178 1277 1190 571 150 113 EcoRI G'AATT_C Cuts at: 5788 6166 5788 Size: 378 8101 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2521 3383 2521 Size: 862 7617 FspI TGC'GCA Cuts at: 6985 6985 Size: 8479 HaeII r_GCGC'y Cuts at: 1678 7626 1678 Size: 5948 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2334 2366 3277 3309 5169 Size: 32 135 32 762 32 911 32 1860 Cuts at: 5169 5193 5201 5225 5282 5314 7120 7152 7194 Size: 24 8 24 57 32 1806 32 42 Cuts at: 7194 7226 1373 Size: 32 2626 Fragments arranged by size: 2626 1860 1806 911 762 135 57 42 32 32 32 32 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 669 1657 1938 2655 4027 5823 6069 669 Size: 988 281 717 1372 1796 246 3079 Fragments arranged by size: 3079 1796 1372 988 717 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8479 HpaI GTT'AAC Cuts at: 6069 6069 Size: 8479 KpnI G_GTAC'C Cuts at: 5804 5804 Size: 8479 MfeI C'AATT_G Cuts at: 6078 6078 Size: 8479 MluI A'CGCG_T Cuts at: 8355 8355 Size: 8479 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2116 3451 5108 7593 7777 1305 Size: 811 1335 1657 2485 184 2007 Fragments arranged by size: 2485 2007 1657 1335 811 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4005 4365 10 Size: 54 1425 343 2173 360 4124 Fragments arranged by size: 4124 2173 1425 360 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3603 4304 4988 6437 6796 6955 518 Size: 648 2437 701 684 1449 359 159 2042 Fragments arranged by size: 2437 2042 1449 701 684 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2162 2280 3851 4929 4941 4971 5157 5895 Size: 556 118 1571 1078 12 30 186 738 Cuts at: 5895 6572 7720 7965 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1571 1148 1078 738 677 556 245 186 118 30 12 NaeI GCC'GGC Cuts at: 4857 4857 Size: 8479 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7927 NdeI CA'TA_TG Cuts at: 387 387 Size: 8479 NgoMIV G'CCGG_C Cuts at: 4855 4855 Size: 8479 NheI G'CTAG_C Cuts at: 1052 3788 1052 Size: 2736 5743 NotI GC'GGCC_GC Cuts at: 5926 5926 Size: 8479 NsiI A_TGCA'T Cuts at: 5101 5101 Size: 8479 NspI r_CATG'y Cuts at: 1132 1229 3608 4414 5103 5837 7382 1132 Size: 97 2379 806 689 734 1545 2229 Fragments arranged by size: 2379 2229 1545 806 734 689 97 PasI CC'CwG_GG Cuts at: 1348 2077 1348 Size: 729 7750 PciI A'CATG_T Cuts at: 7378 7378 Size: 8479 PflMI CCAn_nnn'nTGG Cuts at: 1108 2597 4117 4700 4720 5729 1108 Size: 1489 1520 583 20 1009 3858 Fragments arranged by size: 3858 1520 1489 1009 583 20 PfoI T'CCnGG_A Cuts at: 4403 4403 Size: 8479 PmeI GTTT'AAAC Cuts at: 5783 5783 Size: 8479 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6548 6580 8061 8093 6548 Size: 32 1481 32 6934 Fragments arranged by size: 6934 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2072 2335 2906 5373 2072 Size: 263 571 2467 5178 Fragments arranged by size: 5178 2467 571 263 PshAI GACnn'nnGTC Cuts at: 1613 4429 1613 Size: 2816 5663 PsiI TTA'TAA Cuts at: 6049 6049 Size: 8479 PspOMI G'GGCC_C Cuts at: 2185 4395 2185 Size: 2210 6269 PspXI vC'TCGA_Gb Cuts at: 2513 2513 Size: 8479 PstI C_TGCA'G Cuts at: 830 2893 4330 5831 830 Size: 2063 1437 1501 3478 Fragments arranged by size: 3478 2063 1501 1437 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8479 PvuII CAG'CTG Cuts at: 2280 2280 Size: 8479 SacI G_AGCT'C Cuts at: 2391 4922 2391 Size: 2531 5948 SalI G'TCGA_C Cuts at: 1936 2653 5821 1936 Size: 717 3168 4594 Fragments arranged by size: 4594 3168 717 SanDI GG'GwC_CC Cuts at: 2072 5373 2072 Size: 3301 5178 SapI GCTCTTCn'nnn_ Cuts at: 4190 4190 Size: 8479 SbfI CC_TGCA'GG Cuts at: 5831 5831 Size: 8479 ScaI AGT'ACT Cuts at: 6727 8439 6727 Size: 1712 6767 SfcI C'TryA_G Cuts at: 826 1045 2889 4326 5655 5827 6962 7643 7834 Size: 219 1844 1437 1329 172 1135 681 191 Cuts at: 7834 826 Size: 1471 Fragments arranged by size: 1844 1471 1437 1329 1135 681 219 191 172 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8479 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8479 SmaI CCC'GGG Cuts at: 1872 5806 1872 Size: 3934 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2513 3206 3609 5649 6583 7484 Size: 197 926 570 693 403 2040 934 901 Cuts at: 7484 7746 820 Size: 262 1553 Fragments arranged by size: 2040 1553 934 926 901 693 570 403 262 197 SnaBI TAC'GTA Cuts at: 493 6270 493 Size: 5777 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8479 SphI G_CATG'C Cuts at: 1132 5837 1132 Size: 4705 3774 SspI AAT'ATT Cuts at: 5 52 6403 5 Size: 47 6351 2081 Fragments arranged by size: 6351 2081 47 StuI AGG'CCT Cuts at: 1517 4047 1517 Size: 2530 5949 StyI C'CwwG_G Cuts at: 513 1065 4034 5487 5918 513 Size: 552 2969 1453 431 3074 Fragments arranged by size: 3074 2969 1453 552 431 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2587 4568 4619 6506 6523 6676 6861 Size: 1255 1007 1981 51 1887 17 153 185 Cuts at: 6861 7306 325 Size: 445 1498 Fragments arranged by size: 1981 1887 1498 1255 1007 445 185 153 51 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6725 8437 96 Size: 274 80 33 51 1443 4748 1712 138 Fragments arranged by size: 4748 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4241 4268 4273 4293 4300 4325 4564 4596 8439 Size: 27 5 20 7 25 239 32 3843 Cuts at: 8439 8471 4241 Size: 32 4249 Fragments arranged by size: 4249 3843 239 32 32 27 25 20 7 5 Tth111I GACn'n_nGTC Cuts at: 1316 3119 1316 Size: 1803 6676 XbaI T'CTAG_A Cuts at: 3181 5815 3181 Size: 2634 5845 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2594 2594 Size: 8479 XhoI C'TCGA_G Cuts at: 1943 2513 5649 1943 Size: 570 3136 4773 Fragments arranged by size: 4773 3136 570 XmaI C'CCGG_G Cuts at: 1870 5804 1870 Size: 3934 4545 XmnI GAAnn'nnTTC Cuts at: 2501 3397 3623 5751 6608 2501 Size: 896 226 2128 857 4372 Fragments arranged by size: 4372 2128 896 857 226 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6287 276 Size: 53 83 186 936 4753 2468 Fragments arranged by size: 4753 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I BstXI BstZ17I EcoRV FseI FspAI KasI NarI NruI PacI PmlI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI