Restriction map for
pFN21AE0200
##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccIII AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BalI BamHI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BssSI Bst1107I BstAPI BstBI
BstXI BstZ17I Bsu36I CelII Cfr10I
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoRI EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI MamI MfeI
MluI MluNI MroI MscI MslI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI RcaI RsrII SacI SalI
SapI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI StuI SwaI Van91I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AclI 55 AACGTT AA^CGTT
AcsI 199 AAATTC [GA]^AATT[CT]
AcyI 174 GGCGCC G[GA]^CG[CT]C
AfaI 573 GTAC GT^AC
ApoI 199 AAATTC [GA]^AATT[CT]
Asp700I 195 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 760 GTGCTC G[AT]GC[AT]^C
AvaI 748 CCCGAG C^[CT]CG[GA]G
BbeI 174 GGCGCC GGCGC^C
BbsI 625 GTCTTC GAAGAC(2/6)
BfaI 233 CTAG C^TAG
BpuAI 625 GTCTTC GAAGAC(2/6)
BsaHI 174 GGCGCC G[GA]^CG[CT]C
BsaI 409 GAGACC GGTCTC(1/5)
BsgI 100 GTGCAG GTGCAG(16/14)
BsiHKAI 760 GTGCTC G[AT]GC[AT]^C
BsmFI 228 GTCCC GGGAC(10/14)
BsoBI 748 CCCGAG C^[CT]CG[GA]G
BspDI 829 ATCGAT AT^CGAT
BspMI 575 ACCTGC ACCTGC(4/8)
BsrI 927 ACTGG ACTGG(1/-1)
BssHII 508 GCGCGC G^CGCGC
BstEII 348 GGTGACC G^GT[ACGT]ACC
BstPI 348 GGTGACC G^GT[ACGT]ACC
ClaI 829 ATCGAT AT^CGAT
EarI 1291 GAAGAG CTCTTC(1/4)
EcoO65I 348 GGTGACC G^GT[ACGT]ACC
EcoRV 187 GATATC GAT^ATC
EcoT14I 1217 CCAAGG C^C[AT][AT]GG
HgaI 551 GCGTC GACGC(5/10)
Hin1I 174 GGCGCC G[GA]^CG[CT]C
KasI 174 GGCGCC G^GCGCC
Ksp632I 1291 GAAGAG CTCTTC(1/4)
KspI 540 CCGCGG CCGC^GG
MaeI 233 CTAG C^TAG
MseI 1232 TTAA T^TAA
NarI 174 GGCGCC GG^CGCC
PpuMI 1081 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 55 AACGTT AA^CGTT
PvuII 337 CAGCTG CAG^CTG
RsaI 573 GTAC GT^AC
SacII 540 CCGCGG CCGC^GG
SbfI 576 CCTGCAGG CCTGCA^GG
SfoI 174 GGCGCC GGC^GCC
SmlI 934 CTCAAG C^T[CT][GA]AG
Sse8387I 576 CCTGCAGG CCTGCA^GG
StyI 1217 CCAAGG C^C[AT][AT]GG
TfiI 706 GATTC G^A[AT]TC
Tru9I 1232 TTAA T^TAA
Tsp509I 200 AATT ^AATT
XmnI 195 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AspI 723 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1027 GACAGTGTC
AvaII 930 GGTCC G^G[AT]CC
1082 GGTCC
BanI 174 GGCGCC G^G[CT][GA]CC
941 GGTGCC
BanII 247 GGGCTC G[GA]GC[CT]^C
751 GAGCCC
BsiEI 329 CGGCCG CG[GA][CT]^CG
461 CGGCCG
BsmBI 477 CGTCTC CGTCTC(1/5)
948 CGTCTC
BsrBI 240 CCGCTC CCGCTC(-3/-3)
450 GAGCGG
BstF5I 432 CATCC GGATG(2/0)
1251 CATCC
BstYI 582 GGATCT [GA]^GATC[CT]
1184 AGATCC
DraII 1081 AGGTCCT [GA]G^G[ACGT]CC[CT]
1211 AGGCCCC
DsaI 326 CCACGG C^C[GA][CT]GG
540 CCGCGG
EagI 329 CGGCCG C^GGCCG
461 CGGCCG
EclXI 329 CGGCCG C^GGCCG
461 CGGCCG
Eco52I 329 CGGCCG C^GGCCG
461 CGGCCG
EcoO109I 1081 AGGTCCT [GA]G^G[ACGT]CC[CT]
1211 AGGCCCC
FokI 432 CATCC GGATG(9/13)
1251 CATCC
MflI 582 GGATCT [GA]^GATC[CT]
1184 AGATCC
NlaIII 372 CATG CATG^
766 CATG
PflFI 723 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1027 GACAGTGTC
PstI 163 CTGCAG CTGCA^G
577 CTGCAG
SfaNI 45 GATGC GCATC(5/9)
608 GCATC
SfcI 163 CTGCAG C^T[GA][CT]AG
577 CTGCAG
TspRI 926 CACTG CA[GC]TG[ACGT][ACGT]^
1029 CAGTG
Tth111I 723 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1027 GACAGTGTC
XhoII 582 GGATCT [GA]^GATC[CT]
1184 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI Bsp1286I
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIV PleI
Sau3AI Sau96I ScrFI TaqI TseI
Tsp45I TthHB8I