Restriction map for pFN21AE0200
 (Circular) (Six-base) MAPSORT of: pFN21AE0200.seq  Check: 6700  from: 1  to: 6067



pFN21AE0200  6067 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:56  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3877     278 
   Size:         53      83     186     936    2341    2468

  Fragments arranged by size: 

                2468    2341     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3410    1937 
   Size:       1473    4594
 
Acc65I G'GTAC_C

Cuts at:   3388    3388 
   Size:       6067
 
AclI AA'CG_TT

Cuts at:   1563    2061    4194    4567    1563 
   Size:        498    2133     373    3063

  Fragments arranged by size: 

                3063    2133     498     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3315    4130    5514    5782    1632 
   Size:       1683     815    1384     268    1917

  Fragments arranged by size: 

                1917    1683    1384     815     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5870
 
AflIII A'CryG_T

Cuts at:   1203    4966    5943    1203 
   Size:       3763     977    1327

  Fragments arranged by size: 

                3763    1327     977
 
AgeI A'CCGG_T

Cuts at:   4898    4898 
   Size:       6067
 
AhdI GACnn_n'nnGTC

Cuts at:   4796    4796 
   Size:       6067
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       6067
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6067
 
AlwNI CAG_nnn'CTG

Cuts at:   2559    2590    2885    3810    5382    2559 
   Size:         31     295     925    1572    3244

  Fragments arranged by size: 

                3244    1572     925     295      31
 
ApaLI G'TGCA_C

Cuts at:   4124    5280    4124 
   Size:       1156    4911
 
ApoI r'AATT_y

Cuts at:   1493    2204    3376    3602    3754    5863    1493 
   Size:        711    1172     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1172     711     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4621     160 
   Size:        872    3589    1606

  Fragments arranged by size: 

                3589    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6067
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2753    3392    1870 
   Size:         73     810     639    4545

  Fragments arranged by size: 

                4545     810     639      73
 
BamHI G'GATC_C

Cuts at:   3397    3764    3397 
   Size:        367    5700
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2179    2946    3388    4843     618 
   Size:        325     217     627     392     767     442    1455    1842

  Fragments arranged by size: 

                1842    1455     767     627     442     392     325     217
            
 
BanII G_rGCy'C

Cuts at:   1391    2256    2760    3386    1391 
   Size:        865     504     626    4072

  Fragments arranged by size: 

                4072     865     626     504
 
BbeI G_GCGC'C

Cuts at:   2183    2183 
   Size:       6067
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2623     928 
   Size:        971     724    4372

  Fragments arranged by size: 

                4372     971     724
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4258    4292    1903 
   Size:         34    2321      34    3678

  Fragments arranged by size: 

                3678    2321      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3959    5169    3959 
   Size:       1210    4857
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6067
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4678     136 
   Size:        107     122      71     631     605    3006    1525

  Fragments arranged by size: 

                3006    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   6062    6062 
   Size:       6067
 
BlpI GC'TnA_GC

Cuts at:   3484    3484 
   Size:       6067
 
Bme1580I G_kGCm'C

Cuts at:   1790    4128    5284    1790 
   Size:       2338    1156    2573

  Fragments arranged by size: 

                2573    2338    1156
 
BmgBI CAC'GTC

Cuts at:   1318    2953    1318 
   Size:       1635    4432
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3798    4756     442 
   Size:        416      53      83     399      24    2381     958    1753

  Fragments arranged by size: 

                2381    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6067
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2880    2880 
   Size:       6067
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2366    2554    2671    2922    4727    1239 
   Size:       1127     188     117     251    1805    2579

  Fragments arranged by size: 

                2579    1805    1127     251     188     117
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2924    4192    5057    5355    2924 
   Size:       1268     865     298    3636

  Fragments arranged by size: 

                3636    1268     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2408    4730     882 
   Size:        617     909    2322    2219

  Fragments arranged by size: 

                2322    2219     909     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3858     493 
   Size:        711    2654    2702

  Fragments arranged by size: 

                2702    2654     711
 
BsaBI GATnn'nnATC

Cuts at:   6061    6061 
   Size:       6067
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2180    3874    4256    4891 
   Size:         53      83     186     936     647    1694     382     635

Cuts at:   4891     275 
   Size:       1451

  Fragments arranged by size: 

                1694    1451     936     647     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   4500    4898    5172    5319    5730    4500 
   Size:        398     274     147     411    4837

  Fragments arranged by size: 

                4837     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2364    2394    1178 
   Size:         30     165      30     961      30    4851

  Fragments arranged by size: 

                4851     961     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2144    2147    2385    2713    2789    2822    2910    3116 
   Size:        958       3     238     328      76      33      88     206

Cuts at:   3116    3160    3290    1186 
   Size:         44     130    3963

  Fragments arranged by size: 

                3963     958     328     238     206     130      88      76
                  44      33       3
 
BseYI C'CCAG_C

Cuts at:   2183    2365    5270    5766    2183 
   Size:        182    2905     496    2484

  Fragments arranged by size: 

                2905    2484     496     182
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2126    1163 
   Size:        573     390    5104

  Fragments arranged by size: 

                5104     573     390
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2337    2469    3517    4278    5306    1540 
   Size:        275     186     336     132    1048     761    1028    2301

  Fragments arranged by size: 

                2301    1048    1028     761     336     275     186     132
            
 
BsiHKAI G_wGCw'C

Cuts at:   2769    4128    4213    5284    2769 
   Size:       1359      85    1071    3552

  Fragments arranged by size: 

                3552    1359    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3578    3671    3578 
   Size:         93    5974
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2488    2959    2488 
   Size:        471    5596
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2256    2760    2769    3386    4128    4213    5284 
   Size:        399     466     504       9     617     742      85    1071

Cuts at:   5284    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     617     504     466     399      85
                   9
 
BspEI T'CCGG_A

Cuts at:   5730    5730 
   Size:       6067
 
BspHI T'CATG_A

Cuts at:   3956    5686    3956 
   Size:       1730    4337
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2589    3422     844 
   Size:       1745     833    3489

  Fragments arranged by size: 

                3489    1745     833
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2247    2457    3954    1394 
   Size:        429     424     210    1497    3507

  Fragments arranged by size: 

                3507    1497     429     424     210
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4562    4736      65 
   Size:       1183    3314     174    1396

  Fragments arranged by size: 

                3314    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4711    4898    1700 
   Size:        110    2901     187    2869

  Fragments arranged by size: 

                2901    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6067
 
BssHII G'CGCG_C

Cuts at:   1925    2513    1925 
   Size:        588    5479
 
BssSI C'ACGA_G

Cuts at:   4127    5139    4127 
   Size:       1012    5055
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       6067
 
BstBI TT'CG_AA

Cuts at:   4871    4871 
   Size:       6067
 
BstEII G'GTnAC_C

Cuts at:   1184    2353    1184 
   Size:       1169    4898
 
BstYI r'GATC_y

Cuts at:   1972    2587    3189    3397    3764    4149    4166    5618    5704 
   Size:        615     602     208     367     385      17    1452      86

Cuts at:   5704    5716    6062    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     615     602     385     367     346     208
                  86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2331    2545     513 
   Size:        552     939     327     214    4035

  Fragments arranged by size: 

                4035     939     552     327     214
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5328

  Fragments arranged by size: 

                5328     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3583    4377    4397    3583 
   Size:        794      20    5253

  Fragments arranged by size: 

                5253     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    2835    1554 
   Size:       1281    4786
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6032
 
DraI TTT'AAA

Cuts at:   3371    3371 
   Size:       6067
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5074     809 
   Size:        507    3758    1802

  Fragments arranged by size: 

                3758    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2334    2466    2548 
   Size:         54    1425      50     275      18     504     132      82

Cuts at:   2548    3514    4403    6032       8 
   Size:        966     889    1629      43

  Fragments arranged by size: 

                1629    1425     966     889     504     275     132      82
                  54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2334    2466    3514    1537 
   Size:        275     522     132    1048    4090

  Fragments arranged by size: 

                4090    1048     522     275     132
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3291    3997    1353 
   Size:       1938     706    3423

  Fragments arranged by size: 

                3423    1938     706
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4654    5028    5174     387 
   Size:       1064     138    3065     374     146    1280

  Fragments arranged by size: 

                3065    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2366    2554    2671    2922    3315    4130    4727 
   Size:        393     734     188     117     251     393     815     597

Cuts at:   4727    5514    5782    1239 
   Size:        787     268    1524

  Fragments arranged by size: 

                1524     815     787     734     597     393     393     268
                 251     188     117
 
EcoO109I rG'GnC_Cy

Cuts at:   3087    3217    3087 
   Size:        130    5937
 
EcoRI G'AATT_C

Cuts at:   3376    3754    3376 
   Size:        378    5689
 
EcoRV GAT'ATC

Cuts at:   2194    2194 
   Size:       6067
 
FspI TGC'GCA

Cuts at:   4573    4573 
   Size:       6067
 
HaeII r_GCGC'y

Cuts at:   1678    2183    2598    2694    2984    3142    5214    1678 
   Size:        505     415      96     290     158    2072    2531

  Fragments arranged by size: 

                2531    2072     505     415     290     158      96
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2084    2116    2642    2674    2873 
   Size:         32     135      32     512      32     526      32     199

Cuts at:   2873    2905    4708    4740    4782    4814    1373 
   Size:         32    1803      32      42      32    2626

  Fragments arranged by size: 

                2626    1803     526     512     199     135      42      32
                  32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3411    3657     669 
   Size:        988     281    1473     246    3079

  Fragments arranged by size: 

                3079    1473     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       6067
 
HpaI GTT'AAC

Cuts at:   3657    3657 
   Size:       6067
 
KasI G'GCGC_C

Cuts at:   2179    2179 
   Size:       6067
 
KpnI G_GTAC'C

Cuts at:   3392    3392 
   Size:       6067
 
MfeI C'AATT_G

Cuts at:   3666    3666 
   Size:       6067
 
MluI A'CGCG_T

Cuts at:   5943    5943 
   Size:       6067
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    3009    3180    5181    5365    1305 
   Size:       1704     171    2001     184    2007

  Fragments arranged by size: 

                2007    2001    1704     184     171
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4245

  Fragments arranged by size: 

                4245    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    4025    4384    4543     518 
   Size:        648    2859     359     159    2042

  Fragments arranged by size: 

                2859    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2344    2526    2547    3483    4160    5308    5553    1606 
   Size:        738     182      21     936     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     936     738     677     245     182      21
            
 
NarI GG'CG_CC

Cuts at:   2180    2180 
   Size:       6067
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4576

  Fragments arranged by size: 

                4576     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       6067
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6067
 
NotI GC'GGCC_GC

Cuts at:   3514    3514 
   Size:       6067
 
NspI r_CATG'y

Cuts at:   1132    1229    3425    4970    1132 
   Size:         97    2196    1545    2229

  Fragments arranged by size: 

                2229    2196    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6067
 
PciI A'CATG_T

Cuts at:   4966    4966 
   Size:       6067
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       6067
 
PfoI T'CCnGG_A

Cuts at:   2439    3192    2439 
   Size:        753    5314
 
PmeI GTTT'AAAC

Cuts at:   3371    3371 
   Size:       6067
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4136    4168    5649    5681    4136 
   Size:         32    1481      32    4522

  Fragments arranged by size: 

                4522    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   3087    3087 
   Size:       6067
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6067
 
PsiI TTA'TAA

Cuts at:   3637    3637 
   Size:       6067
 
PstI C_TGCA'G

Cuts at:    830    2172    2586    3419     830 
   Size:       1342     414     833    3478

  Fragments arranged by size: 

                3478    1342     833     414
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6067
 
PvuII CAG'CTG

Cuts at:   2344    2344 
   Size:       6067
 
SacII CC_GC'GG

Cuts at:   2548    2548 
   Size:       6067
 
SalI G'TCGA_C

Cuts at:   1936    3409    1936 
   Size:       1473    4594
 
SbfI CC_TGCA'GG

Cuts at:   2586    3419    2586 
   Size:        833    5234
 
ScaI AGT'ACT

Cuts at:   4315    6027    4315 
   Size:       1712    4355
 
SfcI C'TryA_G

Cuts at:    826    1045    2168    2582    3415    4550    5231    5422     826 
   Size:        219    1123     414     833    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1123     833     681     414     219     191
            
 
SfoI GGC'GCC

Cuts at:   2181    2181 
   Size:       6067
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6067
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6067
 
SmaI CCC'GGG

Cuts at:   1872    3394    1872 
   Size:       1522    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2939    4171    5072    5334     820 
   Size:        197     926     996    1232     901     262    1553

  Fragments arranged by size: 

                1553    1232     996     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3858     493 
   Size:       3365    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6067
 
SphI G_CATG'C

Cuts at:   1132    3425    1132 
   Size:       2293    3774
 
SspI AAT'ATT

Cuts at:      5      52    3991       5 
   Size:         47    3939    2081

  Fragments arranged by size: 

                3939    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6067
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    3222    3506     513 
   Size:        552     939    1218     284    3074

  Fragments arranged by size: 

                3074    1218     939     552     284
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2372    4094    4111    4264    4449    4894     325 
   Size:       1255     792    1722      17     153     185     445    1498

  Fragments arranged by size: 

                1722    1498    1255     792     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4313    6025      96 
   Size:        274      80      33      51    1443    2336    1712     138

  Fragments arranged by size: 

                2336    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2364    2396    2435    2467    3346    3378    6027    6059    2364 
   Size:         32      39      32     879      32    2649      32    2372

  Fragments arranged by size: 

                2649    2372     879      39      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2731    3035    1316 
   Size:       1415     304    4348

  Fragments arranged by size: 

                4348    1415     304
 
XbaI T'CTAG_A

Cuts at:   3403    3403 
   Size:       6067
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6067
 
XmaI C'CCGG_G

Cuts at:   1870    3392    1870 
   Size:       1522    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2204    4196    2204 
   Size:       1992    4075
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3875     276 
   Size:         53      83     186     936    2341    2468

  Fragments arranged by size: 

                2468    2341     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI EcoO109I    EcoRI    EcoRV     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NarI     NcoI     NdeI     NheI     NotI
     NspI     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI
    PshAI     PsiI     PstI     PvuI    PvuII    SacII     SalI     SbfI
     ScaI     SfcI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI    BbvCI
    BfrBI   Bpu10I    BsiWI    BstXI  BstZ17I   Bsu36I   DraIII  EcoICRI
    EcoNI     FalI     FseI    FspAI     NaeI   NgoMIV     NruI     NsiI
     PacI     PmlI   PspOMI    PspXI     PsrI    RsrII     SacI    SanDI
     SapI    SexAI     SfiI     SrfI     SwaI     XcmI
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