(Circular) (Six-base) MAPSORT of: pFN21AE0200.seq Check: 6700 from: 1 to: 6067 pFN21AE0200 6067 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3877 278 Size: 53 83 186 936 2341 2468 Fragments arranged by size: 2468 2341 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3410 1937 Size: 1473 4594 Acc65I G'GTAC_C Cuts at: 3388 3388 Size: 6067 AclI AA'CG_TT Cuts at: 1563 2061 4194 4567 1563 Size: 498 2133 373 3063 Fragments arranged by size: 3063 2133 498 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3315 4130 5514 5782 1632 Size: 1683 815 1384 268 1917 Fragments arranged by size: 1917 1683 1384 815 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5870 AflIII A'CryG_T Cuts at: 1203 4966 5943 1203 Size: 3763 977 1327 Fragments arranged by size: 3763 1327 977 AgeI A'CCGG_T Cuts at: 4898 4898 Size: 6067 AhdI GACnn_n'nnGTC Cuts at: 4796 4796 Size: 6067 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6067 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6067 AlwNI CAG_nnn'CTG Cuts at: 2559 2590 2885 3810 5382 2559 Size: 31 295 925 1572 3244 Fragments arranged by size: 3244 1572 925 295 31 ApaLI G'TGCA_C Cuts at: 4124 5280 4124 Size: 1156 4911 ApoI r'AATT_y Cuts at: 1493 2204 3376 3602 3754 5863 1493 Size: 711 1172 226 152 2109 1697 Fragments arranged by size: 2109 1697 1172 711 226 152 AseI AT'TA_AT Cuts at: 160 1032 4621 160 Size: 872 3589 1606 Fragments arranged by size: 3589 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 AvaI C'yCGr_G Cuts at: 1870 1943 2753 3392 1870 Size: 73 810 639 4545 Fragments arranged by size: 4545 810 639 73 BamHI G'GATC_C Cuts at: 3397 3764 3397 Size: 367 5700 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2179 2946 3388 4843 618 Size: 325 217 627 392 767 442 1455 1842 Fragments arranged by size: 1842 1455 767 627 442 392 325 217 BanII G_rGCy'C Cuts at: 1391 2256 2760 3386 1391 Size: 865 504 626 4072 Fragments arranged by size: 4072 865 626 504 BbeI G_GCGC'C Cuts at: 2183 2183 Size: 6067 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2623 928 Size: 971 724 4372 Fragments arranged by size: 4372 971 724 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4258 4292 1903 Size: 34 2321 34 3678 Fragments arranged by size: 3678 2321 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3959 5169 3959 Size: 1210 4857 BclI T'GATC_A Cuts at: 1548 1548 Size: 6067 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4678 136 Size: 107 122 71 631 605 3006 1525 Fragments arranged by size: 3006 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6062 6062 Size: 6067 BlpI GC'TnA_GC Cuts at: 3484 3484 Size: 6067 Bme1580I G_kGCm'C Cuts at: 1790 4128 5284 1790 Size: 2338 1156 2573 Fragments arranged by size: 2573 2338 1156 BmgBI CAC'GTC Cuts at: 1318 2953 1318 Size: 1635 4432 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3798 4756 442 Size: 416 53 83 399 24 2381 958 1753 Fragments arranged by size: 2381 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6067 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2880 2880 Size: 6067 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2366 2554 2671 2922 4727 1239 Size: 1127 188 117 251 1805 2579 Fragments arranged by size: 2579 1805 1127 251 188 117 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2924 4192 5057 5355 2924 Size: 1268 865 298 3636 Fragments arranged by size: 3636 1268 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2408 4730 882 Size: 617 909 2322 2219 Fragments arranged by size: 2322 2219 909 617 BsaAI yAC'GTr Cuts at: 493 1204 3858 493 Size: 711 2654 2702 Fragments arranged by size: 2702 2654 711 BsaBI GATnn'nnATC Cuts at: 6061 6061 Size: 6067 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2180 3874 4256 4891 Size: 53 83 186 936 647 1694 382 635 Cuts at: 4891 275 Size: 1451 Fragments arranged by size: 1694 1451 936 647 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4500 4898 5172 5319 5730 4500 Size: 398 274 147 411 4837 Fragments arranged by size: 4837 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2364 2394 1178 Size: 30 165 30 961 30 4851 Fragments arranged by size: 4851 961 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2144 2147 2385 2713 2789 2822 2910 3116 Size: 958 3 238 328 76 33 88 206 Cuts at: 3116 3160 3290 1186 Size: 44 130 3963 Fragments arranged by size: 3963 958 328 238 206 130 88 76 44 33 3 BseYI C'CCAG_C Cuts at: 2183 2365 5270 5766 2183 Size: 182 2905 496 2484 Fragments arranged by size: 2905 2484 496 182 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2126 1163 Size: 573 390 5104 Fragments arranged by size: 5104 573 390 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2337 2469 3517 4278 5306 1540 Size: 275 186 336 132 1048 761 1028 2301 Fragments arranged by size: 2301 1048 1028 761 336 275 186 132 BsiHKAI G_wGCw'C Cuts at: 2769 4128 4213 5284 2769 Size: 1359 85 1071 3552 Fragments arranged by size: 3552 1359 1071 85 BsmI GAATG_Cn' Cuts at: 3578 3671 3578 Size: 93 5974 BsmBI CGTCTCn'nnnn_ Cuts at: 2488 2959 2488 Size: 471 5596 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2256 2760 2769 3386 4128 4213 5284 Size: 399 466 504 9 617 742 85 1071 Cuts at: 5284 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 617 504 466 399 85 9 BspEI T'CCGG_A Cuts at: 5730 5730 Size: 6067 BspHI T'CATG_A Cuts at: 3956 5686 3956 Size: 1730 4337 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2589 3422 844 Size: 1745 833 3489 Fragments arranged by size: 3489 1745 833 BsrBI CCG'CTC Cuts at: 1394 1823 2247 2457 3954 1394 Size: 429 424 210 1497 3507 Fragments arranged by size: 3507 1497 429 424 210 BsrDI GCAATG_nn' Cuts at: 65 1248 4562 4736 65 Size: 1183 3314 174 1396 Fragments arranged by size: 3314 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4711 4898 1700 Size: 110 2901 187 2869 Fragments arranged by size: 2901 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6067 BssHII G'CGCG_C Cuts at: 1925 2513 1925 Size: 588 5479 BssSI C'ACGA_G Cuts at: 4127 5139 4127 Size: 1012 5055 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6067 BstBI TT'CG_AA Cuts at: 4871 4871 Size: 6067 BstEII G'GTnAC_C Cuts at: 1184 2353 1184 Size: 1169 4898 BstYI r'GATC_y Cuts at: 1972 2587 3189 3397 3764 4149 4166 5618 5704 Size: 615 602 208 367 385 17 1452 86 Cuts at: 5704 5716 6062 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 615 602 385 367 346 208 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2331 2545 513 Size: 552 939 327 214 4035 Fragments arranged by size: 4035 939 552 327 214 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5328 Fragments arranged by size: 5328 683 56 BtsI GCAGTG_nn' Cuts at: 3583 4377 4397 3583 Size: 794 20 5253 Fragments arranged by size: 5253 794 20 ClaI AT'CG_AT Cuts at: 1554 2835 1554 Size: 1281 4786 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6032 DraI TTT'AAA Cuts at: 3371 3371 Size: 6067 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5074 809 Size: 507 3758 1802 Fragments arranged by size: 3758 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2334 2466 2548 Size: 54 1425 50 275 18 504 132 82 Cuts at: 2548 3514 4403 6032 8 Size: 966 889 1629 43 Fragments arranged by size: 1629 1425 966 889 504 275 132 82 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2334 2466 3514 1537 Size: 275 522 132 1048 4090 Fragments arranged by size: 4090 1048 522 275 132 EarI CTCTTCn'nnn_ Cuts at: 1353 3291 3997 1353 Size: 1938 706 3423 Fragments arranged by size: 3423 1938 706 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4654 5028 5174 387 Size: 1064 138 3065 374 146 1280 Fragments arranged by size: 3065 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2366 2554 2671 2922 3315 4130 4727 Size: 393 734 188 117 251 393 815 597 Cuts at: 4727 5514 5782 1239 Size: 787 268 1524 Fragments arranged by size: 1524 815 787 734 597 393 393 268 251 188 117 EcoO109I rG'GnC_Cy Cuts at: 3087 3217 3087 Size: 130 5937 EcoRI G'AATT_C Cuts at: 3376 3754 3376 Size: 378 5689 EcoRV GAT'ATC Cuts at: 2194 2194 Size: 6067 FspI TGC'GCA Cuts at: 4573 4573 Size: 6067 HaeII r_GCGC'y Cuts at: 1678 2183 2598 2694 2984 3142 5214 1678 Size: 505 415 96 290 158 2072 2531 Fragments arranged by size: 2531 2072 505 415 290 158 96 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2084 2116 2642 2674 2873 Size: 32 135 32 512 32 526 32 199 Cuts at: 2873 2905 4708 4740 4782 4814 1373 Size: 32 1803 32 42 32 2626 Fragments arranged by size: 2626 1803 526 512 199 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3411 3657 669 Size: 988 281 1473 246 3079 Fragments arranged by size: 3079 1473 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6067 HpaI GTT'AAC Cuts at: 3657 3657 Size: 6067 KasI G'GCGC_C Cuts at: 2179 2179 Size: 6067 KpnI G_GTAC'C Cuts at: 3392 3392 Size: 6067 MfeI C'AATT_G Cuts at: 3666 3666 Size: 6067 MluI A'CGCG_T Cuts at: 5943 5943 Size: 6067 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3009 3180 5181 5365 1305 Size: 1704 171 2001 184 2007 Fragments arranged by size: 2007 2001 1704 184 171 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4245 Fragments arranged by size: 4245 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4025 4384 4543 518 Size: 648 2859 359 159 2042 Fragments arranged by size: 2859 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2344 2526 2547 3483 4160 5308 5553 1606 Size: 738 182 21 936 677 1148 245 2120 Fragments arranged by size: 2120 1148 936 738 677 245 182 21 NarI GG'CG_CC Cuts at: 2180 2180 Size: 6067 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4576 Fragments arranged by size: 4576 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6067 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6067 NotI GC'GGCC_GC Cuts at: 3514 3514 Size: 6067 NspI r_CATG'y Cuts at: 1132 1229 3425 4970 1132 Size: 97 2196 1545 2229 Fragments arranged by size: 2229 2196 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6067 PciI A'CATG_T Cuts at: 4966 4966 Size: 6067 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6067 PfoI T'CCnGG_A Cuts at: 2439 3192 2439 Size: 753 5314 PmeI GTTT'AAAC Cuts at: 3371 3371 Size: 6067 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4136 4168 5649 5681 4136 Size: 32 1481 32 4522 Fragments arranged by size: 4522 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3087 3087 Size: 6067 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6067 PsiI TTA'TAA Cuts at: 3637 3637 Size: 6067 PstI C_TGCA'G Cuts at: 830 2172 2586 3419 830 Size: 1342 414 833 3478 Fragments arranged by size: 3478 1342 833 414 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6067 PvuII CAG'CTG Cuts at: 2344 2344 Size: 6067 SacII CC_GC'GG Cuts at: 2548 2548 Size: 6067 SalI G'TCGA_C Cuts at: 1936 3409 1936 Size: 1473 4594 SbfI CC_TGCA'GG Cuts at: 2586 3419 2586 Size: 833 5234 ScaI AGT'ACT Cuts at: 4315 6027 4315 Size: 1712 4355 SfcI C'TryA_G Cuts at: 826 1045 2168 2582 3415 4550 5231 5422 826 Size: 219 1123 414 833 1135 681 191 1471 Fragments arranged by size: 1471 1135 1123 833 681 414 219 191 SfoI GGC'GCC Cuts at: 2181 2181 Size: 6067 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6067 SmaI CCC'GGG Cuts at: 1872 3394 1872 Size: 1522 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2939 4171 5072 5334 820 Size: 197 926 996 1232 901 262 1553 Fragments arranged by size: 1553 1232 996 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3858 493 Size: 3365 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6067 SphI G_CATG'C Cuts at: 1132 3425 1132 Size: 2293 3774 SspI AAT'ATT Cuts at: 5 52 3991 5 Size: 47 3939 2081 Fragments arranged by size: 3939 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6067 StyI C'CwwG_G Cuts at: 513 1065 2004 3222 3506 513 Size: 552 939 1218 284 3074 Fragments arranged by size: 3074 1218 939 552 284 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2372 4094 4111 4264 4449 4894 325 Size: 1255 792 1722 17 153 185 445 1498 Fragments arranged by size: 1722 1498 1255 792 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4313 6025 96 Size: 274 80 33 51 1443 2336 1712 138 Fragments arranged by size: 2336 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2364 2396 2435 2467 3346 3378 6027 6059 2364 Size: 32 39 32 879 32 2649 32 2372 Fragments arranged by size: 2649 2372 879 39 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2731 3035 1316 Size: 1415 304 4348 Fragments arranged by size: 4348 1415 304 XbaI T'CTAG_A Cuts at: 3403 3403 Size: 6067 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6067 XmaI C'CCGG_G Cuts at: 1870 3392 1870 Size: 1522 4545 XmnI GAAnn'nnTTC Cuts at: 2204 4196 2204 Size: 1992 4075 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3875 276 Size: 53 83 186 936 2341 2468 Fragments arranged by size: 2468 2341 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI