Restriction map for
pFN21AE0200
(Linear) (Six-base) MAPSORT of: KIEE0200.seq Check: 2893 from: 1 to: 1362
KIEE0200.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AclI AA'CG_TT
Cuts at: 0 56 1362
Size: 56 1306
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1310 1362
Size: 1310 52
AlwNI CAG_nnn'CTG
Cuts at: 0 554 585 880 1362
Size: 554 31 295 482
Fragments arranged by size:
554 482 295 31
ApoI r'AATT_y
Cuts at: 0 199 1362
Size: 199 1163
AvaI C'yCGr_G
Cuts at: 0 748 1362
Size: 748 614
BanI G'GyrC_C
Cuts at: 0 174 941 1362
Size: 174 767 421
Fragments arranged by size:
767 421 174
BanII G_rGCy'C
Cuts at: 0 251 755 1362
Size: 251 504 607
Fragments arranged by size:
607 504 251
BbeI G_GCGC'C
Cuts at: 0 178 1362
Size: 178 1184
BbsI GAAGACnn'nnnn_
Cuts at: 0 618 1362
Size: 618 744
BmgBI CAC'GTC
Cuts at: 0 948 1362
Size: 948 414
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 875 1362
Size: 875 487
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 549 666 917 1362
Size: 361 188 117 251 445
Fragments arranged by size:
445 361 251 188 117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 919 1362
Size: 919 443
BsaI GGTCTCn'nnnn_
Cuts at: 0 403 1362
Size: 403 959
BsaHI Gr'CG_yC
Cuts at: 0 175 1362
Size: 175 1187
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 359 389 1362
Size: 359 30 973
Fragments arranged by size:
973 359 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 139 142 380 708 784 817 905 1111
Size: 139 3 238 328 76 33 88 206
Cuts at: 1111 1155 1285 1362
Size: 44 130 77
Fragments arranged by size:
328 238 206 139 130 88 77 76
44 33 3
BseYI C'CCAG_C
Cuts at: 0 178 360 1362
Size: 178 182 1002
Fragments arranged by size:
1002 182 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 1362
Size: 121 1241
BsiEI CG_ry'CG
Cuts at: 0 332 464 1362
Size: 332 132 898
Fragments arranged by size:
898 332 132
BsiHKAI G_wGCw'C
Cuts at: 0 764 1362
Size: 764 598
BsmBI CGTCTCn'nnnn_
Cuts at: 0 483 954 1362
Size: 483 471 408
Fragments arranged by size:
483 471 408
Bsp1286I G_dGCh'C
Cuts at: 0 251 755 764 1362
Size: 251 504 9 598
Fragments arranged by size:
598 504 251 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 584 1362
Size: 584 778
BsrBI CCG'CTC
Cuts at: 0 242 452 1362
Size: 242 210 910
Fragments arranged by size:
910 242 210
BssHII G'CGCG_C
Cuts at: 0 508 1362
Size: 508 854
BstEII G'GTnAC_C
Cuts at: 0 348 1362
Size: 348 1014
BstYI r'GATC_y
Cuts at: 0 582 1184 1362
Size: 582 602 178
Fragments arranged by size:
602 582 178
BtgI C'CryG_G
Cuts at: 0 326 540 1362
Size: 326 214 822
Fragments arranged by size:
822 326 214
ClaI AT'CG_AT
Cuts at: 0 830 1362
Size: 830 532
EaeI y'GGCC_r
Cuts at: 0 329 461 543 1362
Size: 329 132 82 819
Fragments arranged by size:
819 329 132 82
EagI C'GGCC_G
Cuts at: 0 329 461 1362
Size: 329 132 901
Fragments arranged by size:
901 329 132
EarI CTCTTCn'nnn_
Cuts at: 0 1286 1362
Size: 1286 76
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 549 666 917 1310 1362
Size: 361 188 117 251 393 52
Fragments arranged by size:
393 361 251 188 117 52
EcoO109I rG'GnC_Cy
Cuts at: 0 1082 1212 1362
Size: 1082 130 150
Fragments arranged by size:
1082 150 130
EcoRV GAT'ATC
Cuts at: 0 189 1362
Size: 189 1173
HaeII r_GCGC'y
Cuts at: 0 178 593 689 979 1137 1362
Size: 178 415 96 290 158 225
Fragments arranged by size:
415 290 225 178 158 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 79 111 637 669 868 900 1362
Size: 79 32 526 32 199 32 462
Fragments arranged by size:
526 462 199 79 32 32 32
KasI G'GCGC_C
Cuts at: 0 174 1362
Size: 174 1188
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1004 1175 1362
Size: 1004 171 187
Fragments arranged by size:
1004 187 171
MspA1I CmG'CkG
Cuts at: 0 339 521 542 1362
Size: 339 182 21 820
Fragments arranged by size:
820 339 182 21
NarI GG'CG_CC
Cuts at: 0 175 1362
Size: 175 1187
PfoI T'CCnGG_A
Cuts at: 0 434 1187 1362
Size: 434 753 175
Fragments arranged by size:
753 434 175
PpuMI rG'GwC_Cy
Cuts at: 0 1082 1362
Size: 1082 280
PstI C_TGCA'G
Cuts at: 0 167 581 1362
Size: 167 414 781
Fragments arranged by size:
781 414 167
PvuII CAG'CTG
Cuts at: 0 339 1362
Size: 339 1023
SacII CC_GC'GG
Cuts at: 0 543 1362
Size: 543 819
SbfI CC_TGCA'GG
Cuts at: 0 581 1362
Size: 581 781
SfcI C'TryA_G
Cuts at: 0 163 577 1362
Size: 163 414 785
Fragments arranged by size:
785 414 163
SfoI GGC'GCC
Cuts at: 0 176 1362
Size: 176 1186
SmlI C'TyrA_G
Cuts at: 0 934 1362
Size: 934 428
StyI C'CwwG_G
Cuts at: 0 1217 1362
Size: 1217 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 367 1362
Size: 367 995
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 359 391 430 462 1341 1362
Size: 359 32 39 32 879 21
Fragments arranged by size:
879 359 39 32 32 21
Tth111I GACn'n_nGTC
Cuts at: 0 726 1030 1362
Size: 726 304 332
Fragments arranged by size:
726 332 304
XmnI GAAnn'nnTTC
Cuts at: 0 199 1362
Size: 199 1163
Enzymes that do cut:
AclI AcuI AlwNI ApoI AvaI BanI BanII BbeI
BbsI BmgBI BplI BpmI BpuEI BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BssHII BstEII BstYI BtgI ClaI EaeI EagI
EarI Eco57MI EcoO109I EcoRV HaeII Hin4I KasI MmeI
MspA1I NarI PfoI PpuMI PstI PvuII SacII SbfI
SfcI SfoI SmlI StyI TaqII TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BmtI Bpu10I
BsaAI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstZ17I Bsu36I
BtgZI BtsI CspCI DraI DraIII DrdI EciI EcoICRI
EcoNI EcoRI FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MscI MslI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SalI SanDI
SapI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI XbaI
XcmI XhoI XmaI ZraI