Restriction map for
pFN21AB0499
##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
AlwNI Aor51HI AscI AseI AsnI
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BfrI BglI BlnI BlpI
Bpu1102I BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
EcoO65I EcoRV EcoT22I FseI FspI
HindIII KasI MluI MroI MslI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SpeI
Sse8387I SspBI StuI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 65 enzymes) #
###################################
AatII 503 GACGTC GACGT^C
Acc65I 223 GGTACC G^GTACC
AccI 1383 GTCTAC GT^[AC][GT]AC
AccII 21 CGCG CG^CG
AcyI 503 GACGTC G[GA]^CG[CT]C
AflIII 802 ACGTGT A^C[GA][CT]GT
Alw44I 488 GTGCAC G^TGCAC
AocI 973 CCTCAGG CC^T[ACGT]AGG
ApaI 24 GGGCCC GGGCC^C
ApaLI 488 GTGCAC G^TGCAC
Asp700I 1181 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 223 GGTACC G^GTACC
AspHI 488 GTGCAC G[AT]GC[AT]^C
AvaII 1015 GGACC G^G[AT]CC
BalI 1667 TGGCCA TGG^CCA
BanI 223 GGTACC G^G[CT][GA]CC
BanII 24 GGGCCC G[GA]GC[CT]^C
BclI 111 TGATCA T^GATCA
BglII 2118 AGATCT A^GATCT
BsaAI 1087 TACGTA [CT]AC^GT[GA]
BsaBI 1269 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 503 GACGTC G[GA]^CG[CT]C
BseRI 35 GAGGAG GAGGAG(10/8)
BsiHKAI 488 GTGCAC G[AT]GC[AT]^C
BspHI 947 TCATGA T^CATGA
BspMI 1482 ACCTGC ACCTGC(4/8)
BstUI 21 CGCG CG^CG
BstYI 2118 AGATCT [GA]^GATC[CT]
Bsu36I 973 CCTCAGG CC^T[ACGT]AGG
CfoI 1064 GCGC GCG^C
DsaI 20 CCGCGG C^C[GA][CT]GG
EaeI 1667 TGGCCA [CT]^GGCC[GA]
Eco81I 973 CCTCAGG CC^T[ACGT]AGG
EcoNI 2080 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 535 GAATTC G^AATTC
EcoT14I 1777 CCAAGG C^C[AT][AT]GG
FbaI 111 TGATCA T^GATCA
HaeII 1063 AGCGCC [GA]GCGC^[CT]
HgaI 1195 GACGC GACGC(5/10)
HhaI 1064 GCGC GCG^C
Hin1I 503 GACGTC G[GA]^CG[CT]C
HinP1I 1064 GCGC G^CGC
HpaI 141 GTTAAC GTT^AAC
KpnI 223 GGTACC GGTAC^C
KspI 20 CCGCGG CCGC^GG
MamI 1269 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 2118 AGATCT [GA]^GATC[CT]
MluNI 1667 TGGCCA TGG^CCA
MscI 1667 TGGCCA TGG^CCA
MspA1I 20 CCGCGG C[AC]G^C[GT]G
MvnI 21 CGCG CG^CG
PflMI 607 CCATCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1622 CTGCAG CTGCA^G
RcaI 947 TCATGA T^CATGA
SacII 20 CCGCGG CCGC^GG
SapI 347 GCTCTTC GCTCTTC(1/4)
SfcI 1622 CTGCAG C^T[GA][CT]AG
SnaBI 1087 TACGTA TAC^GTA
SphI 343 GCATGC GCATG^C
SspI 524 AATATT AAT^ATT
StyI 1777 CCAAGG C^C[AT][AT]GG
Tsp45I 2071 GTCAC ^GT[GC]AC
Van91I 607 CCATCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 2118 AGATCT [GA]^GATC[CT]
XmnI 1181 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwI 970 GATCC GGATC(4/5)
2113 GGATC
BbsI 1882 GAAGAC GAAGAC(2/6)
1425 GTCTTC
BcnI 27 CCCGG CC^[GC]GG
796 CCGGG
BfaI 746 CTAG C^TAG
2155 CTAG
BmyI 24 GGGCCC G[AGT]GC[ACT]^C
488 GTGCAC
BpmI 1010 CTCCAG CTGGAG(16/14)
2081 CTGGAG
BpuAI 1882 GAAGAC GAAGAC(2/6)
1425 GTCTTC
BsaJI 20 CCGCGG C^C[ACGT][ACGT]GG
1777 CCAAGG
Bsp1286I 24 GGGCCC G[AGT]GC[ACT]^C
488 GTGCAC
BstNI 1012 CCAGG CC^[AT]GG
2080 CCTGG
EcoRII 1012 CCAGG ^CC[AT]GG
2080 CCTGG
FauI 16 CCCGC CCCGC(4/6)
22 GCGGG
HapII 28 CCGG C^CGG
796 CCGG
HincII 141 GTTAAC GT[CT]^[GA]AC
2039 GTCAAC
HindII 141 GTTAAC GT[CT]^[GA]AC
2039 GTCAAC
HpaII 28 CCGG C^CGG
796 CCGG
MaeI 746 CTAG C^TAG
2155 CTAG
MfeI 423 CAATTG C^AATTG
509 CAATTG
MspI 28 CCGG C^CGG
796 CCGG
MunI 423 CAATTG C^AATTG
509 CAATTG
MvaI 1012 CCAGG CC^[AT]GG
2080 CCTGG
NciI 27 CCCGG CC^[GC]GG
796 CCGGG
NspI 343 GCATGC [GA]CATG^[CT]
2196 ACATGC
SfaNI 1944 GATGC GCATC(5/9)
1518 GCATC
SmlI 1844 CTCAAG C^T[CT][GA]AG
1876 CTCAAG
TaqI 118 TCGA T^CGA
2139 TCGA
TthHB8I 118 TCGA T^CGA
2139 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BsiYI BslI BsmAI BsmI
BsrI BssKI BstF5I Cac8I Cfr13I
DdeI DpnII DraI EarI Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MseI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI TfiI Tru9I TseI Tsp509I
TspRI